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The map label for this gene is opuE [H]

Identifier: 74316230

GI number: 74316230

Start: 232336

End: 233763

Strand: Reverse

Name: opuE [H]

Synonym: Tbd_0212

Alternate gene names: 74316230

Gene position: 233763-232336 (Counterclockwise)

Preceding gene: 74316231

Following gene: 74316229

Centisome position: 8.03

GC content: 65.62

Gene sequence:

>1428_bases
GTGCTGATCGGTTTTGTCGCTCTCTACCTCGTCGTGTCGATCGGTATCGGACTGTACGGCGCGACCAAGGTCCATGGCGC
CGGCGATTACATCACCGCGGGACGGGCCCTGCCGATGGTCGTCGTCGTCGCGATGGTCTTCGCGACCTGGTTCGGCGCCG
AAACCGTGCTCGGCATTCCGGCGACCTTCCTCGACGAGAACCTCGGCGCGACGATCGCCGACCCCTTCGGCGCCTCGCTC
GCACTGATCCTGTTCGGTCTGTTCTTCGCGCGTCCGCTGTACCGCATGCAACTGCTGACGCTCGGCGACTTCTTTCGCCA
GCGCTACAACCGCCCGGTCGAGGTGGTCATTTCGTCCGCGATCGCGCTGTCCTACCTCGGCTGGGTGTCGGCACAGGTGG
TCGCGCTGGGGCTGGTGTTCAACGTGCTCTCCGAAGGCCTCGTCACCCAGCCGCAGGGCATCGTGATCGGTGCGTCGGTC
GTGCTGCTGTACACGCTGTTTGGCGGCATGTGGTCGGTGGCGCTGACCACCCTGGTCCAGATGACCGTCATCGTGCTCGG
TCTGCTGTGGATCGCGAAGCTCGTCGGCGACATGCCCGAGGTCGACGGCGTCGCCCCGGTCGTCGCGCACGCCGCAGCGG
CGGGCAAGTTCGAATTCTGGCCACCGCTGGAATGGGCGGCGATCCTGACCTTCGTGGCCGGTCTGCTTACCATGGGGCTC
GGATCGATTCCGCAGCAGGACGTCTTCCAGCGTGCCAACGCCGGGAAAACCGAGCGCATCGCGGTATGGGGCACGCTCGT
CGGTGGCCTGCTGTATTTCCTGTTCGCCGCGGTACCGATCTATCTCACCTATGCCGCGACGCTGGTCGATCCGGCGATGA
CGCAGGCGGCGCTCGCCGAGGACGCGCAGCGCGTGCTGCCCGCCTTCGTCAAGAGCCATCTGCCGCTCTATGCGCAAATC
ATCTTTTATGGCGCGCTGCTGTCGGTGATCATGTCGACCGCGTCGGGCACGCTGCTCGCGCCTTCGGTCACGATTTCGGA
AAACGTCATCCGGGAATTCATGCCCCGGCACGGCATGAACGAAACGCAGCTGCTGTGGATCACGCGCGCCGTCGTTGTCG
CCTTCAGCGTACTCGTCGTCGCCTACTCGCTGTGGTCGCTGCAATCGAGCACCAGCATTCACGAAATGGTCGCGAACGCC
TACAAGGTCACGCTTGCCTGTGCCTTCGTTCCGCTCGTGGCCGGGCTTTACTGGCGACGCGCGAGCAACGTCGGCGCCGG
GCTTGCGATCGCGCTCGGTTTCGTCGTCTGGGTCGCGGTGGAGTTCGTTGCGCCGGACGCCGCGTTGCCACCGCAGTTCG
CCGGCCTGATCGCGAGCGCGCTGGGCATGGTGCTGGGCAGCCTGGCCGTCCCCAAACGCCGTCACTGA

Upstream 100 bases:

>100_bases
CCAGCAGCAGACGCGCAACACATGGCTCGGGGCGATCGCCGTGCTGCTCACCGTGCTGTCGGGGCTCGTGCTCTATTCCA
TCCTGCAGTAGGGGCAAGGC

Downstream 100 bases:

>100_bases
CTGTCGGCGGTTTTTACAAGGCCGGCCCATCCGCGCCGCCACACCTGCCGCTGACTCCACATAGGTCATTGATTAGACTG
GACATTGCCGCGCGCTACAG

Product: sodium/solute symporter

Products: Na (I) [Cytoplasm]; pantothenate [Cytoplasm] [C]

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MLIGFVALYLVVSIGIGLYGATKVHGAGDYITAGRALPMVVVVAMVFATWFGAETVLGIPATFLDENLGATIADPFGASL
ALILFGLFFARPLYRMQLLTLGDFFRQRYNRPVEVVISSAIALSYLGWVSAQVVALGLVFNVLSEGLVTQPQGIVIGASV
VLLYTLFGGMWSVALTTLVQMTVIVLGLLWIAKLVGDMPEVDGVAPVVAHAAAAGKFEFWPPLEWAAILTFVAGLLTMGL
GSIPQQDVFQRANAGKTERIAVWGTLVGGLLYFLFAAVPIYLTYAATLVDPAMTQAALAEDAQRVLPAFVKSHLPLYAQI
IFYGALLSVIMSTASGTLLAPSVTISENVIREFMPRHGMNETQLLWITRAVVVAFSVLVVAYSLWSLQSSTSIHEMVANA
YKVTLACAFVPLVAGLYWRRASNVGAGLAIALGFVVWVAVEFVAPDAALPPQFAGLIASALGMVLGSLAVPKRRH

Sequences:

>Translated_475_residues
MLIGFVALYLVVSIGIGLYGATKVHGAGDYITAGRALPMVVVVAMVFATWFGAETVLGIPATFLDENLGATIADPFGASL
ALILFGLFFARPLYRMQLLTLGDFFRQRYNRPVEVVISSAIALSYLGWVSAQVVALGLVFNVLSEGLVTQPQGIVIGASV
VLLYTLFGGMWSVALTTLVQMTVIVLGLLWIAKLVGDMPEVDGVAPVVAHAAAAGKFEFWPPLEWAAILTFVAGLLTMGL
GSIPQQDVFQRANAGKTERIAVWGTLVGGLLYFLFAAVPIYLTYAATLVDPAMTQAALAEDAQRVLPAFVKSHLPLYAQI
IFYGALLSVIMSTASGTLLAPSVTISENVIREFMPRHGMNETQLLWITRAVVVAFSVLVVAYSLWSLQSSTSIHEMVANA
YKVTLACAFVPLVAGLYWRRASNVGAGLAIALGFVVWVAVEFVAPDAALPPQFAGLIASALGMVLGSLAVPKRRH
>Mature_475_residues
MLIGFVALYLVVSIGIGLYGATKVHGAGDYITAGRALPMVVVVAMVFATWFGAETVLGIPATFLDENLGATIADPFGASL
ALILFGLFFARPLYRMQLLTLGDFFRQRYNRPVEVVISSAIALSYLGWVSAQVVALGLVFNVLSEGLVTQPQGIVIGASV
VLLYTLFGGMWSVALTTLVQMTVIVLGLLWIAKLVGDMPEVDGVAPVVAHAAAAGKFEFWPPLEWAAILTFVAGLLTMGL
GSIPQQDVFQRANAGKTERIAVWGTLVGGLLYFLFAAVPIYLTYAATLVDPAMTQAALAEDAQRVLPAFVKSHLPLYAQI
IFYGALLSVIMSTASGTLLAPSVTISENVIREFMPRHGMNETQLLWITRAVVVAFSVLVVAYSLWSLQSSTSIHEMVANA
YKVTLACAFVPLVAGLYWRRASNVGAGLAIALGFVVWVAVEFVAPDAALPPQFAGLIASALGMVLGSLAVPKRRH

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI11141885, Length=420, Percent_Identity=26.9047619047619, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI87082237, Length=448, Percent_Identity=25.6696428571429, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787251, Length=385, Percent_Identity=23.3766233766234, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17539284, Length=422, Percent_Identity=23.2227488151659, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648033, Length=407, Percent_Identity=23.5872235872236, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356865, Length=407, Percent_Identity=23.5872235872236, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 50583; Mature: 50583

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGFVALYLVVSIGIGLYGATKVHGAGDYITAGRALPMVVVVAMVFATWFGAETVLGIP
CHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHCCHHHHHHHHHHHHHHHHHCCHHHHCCC
ATFLDENLGATIADPFGASLALILFGLFFARPLYRMQLLTLGDFFRQRYNRPVEVVISSA
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IALSYLGWVSAQVVALGLVFNVLSEGLVTQPQGIVIGASVVLLYTLFGGMWSVALTTLVQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
MTVIVLGLLWIAKLVGDMPEVDGVAPVVAHAAAAGKFEFWPPLEWAAILTFVAGLLTMGL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
GSIPQQDVFQRANAGKTERIAVWGTLVGGLLYFLFAAVPIYLTYAATLVDPAMTQAALAE
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAQRVLPAFVKSHLPLYAQIIFYGALLSVIMSTASGTLLAPSVTISENVIREFMPRHGMN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCC
ETQLLWITRAVVVAFSVLVVAYSLWSLQSSTSIHEMVANAYKVTLACAFVPLVAGLYWRR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASNVGAGLAIALGFVVWVAVEFVAPDAALPPQFAGLIASALGMVLGSLAVPKRRH
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLIGFVALYLVVSIGIGLYGATKVHGAGDYITAGRALPMVVVVAMVFATWFGAETVLGIP
CHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHCCHHHHHHHHHHHHHHHHHCCHHHHCCC
ATFLDENLGATIADPFGASLALILFGLFFARPLYRMQLLTLGDFFRQRYNRPVEVVISSA
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IALSYLGWVSAQVVALGLVFNVLSEGLVTQPQGIVIGASVVLLYTLFGGMWSVALTTLVQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
MTVIVLGLLWIAKLVGDMPEVDGVAPVVAHAAAAGKFEFWPPLEWAAILTFVAGLLTMGL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
GSIPQQDVFQRANAGKTERIAVWGTLVGGLLYFLFAAVPIYLTYAATLVDPAMTQAALAE
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAQRVLPAFVKSHLPLYAQIIFYGALLSVIMSTASGTLLAPSVTISENVIREFMPRHGMN
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCC
ETQLLWITRAVVVAFSVLVVAYSLWSLQSSTSIHEMVANAYKVTLACAFVPLVAGLYWRR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASNVGAGLAIALGFVVWVAVEFVAPDAALPPQFAGLIASALGMVLGSLAVPKRRH
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; pantothenate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + pantothenate [Periplasm] = Na (I) [Cytoplasm] + pantothenate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]