Click here to switch to the map view.

The map label for this gene is gcvH

Identifier: 74316195

GI number: 74316195

Start: 189772

End: 190161

Strand: Reverse

Name: gcvH

Synonym: Tbd_0177

Alternate gene names: 74316195

Gene position: 190161-189772 (Counterclockwise)

Preceding gene: 74316196

Following gene: 74316194

Centisome position: 6.54

GC content: 65.64

Gene sequence:

>390_bases
ATGTCCATCCCCGCAGACCTCAAATACACGCAGAGCCACGAATGGGTACGCGCCGAAGCCGACGGCAGCGTCACGGTAGG
CATCACCCACCACGCCCAGGACCTCCTCGGCGACATGGTGTTCATCGAGAACCCGGCCGTCGGCCGCACGCTCGCCAAGG
GCGAAGAGTGCGCCGTCGTCGAGTCGGTGAAGGCCGCATCCGACGTCTATGCGCCGGTCGCCGGCGAAGTAATCGCCGCC
AACGGCGAAGTCGAATCCAGCCCCGAGTCGGTGAACCAGGACGCCTACACCGCGTGGCTGTTCAAGATCAAGCCGGCCAA
CCCCGCCGACGTCGACGCACTGCTCGACGCCGCGGCGTACCAGAAGGTGGTCGAGTCGGAAGCACATTGA

Upstream 100 bases:

>100_bases
CGGGCAAAGCCCGCTCGTCCCCTGAGTCCTGACCCCTGAATCCTGACCCCTGAATCCTGACGCCTGATAGCAACCATAAC
GACCCAAGGAAGCCCGACAC

Downstream 100 bases:

>100_bases
GGAGGGGCCAGGATTCAGGGGTCAGGATTCAGGGAATGTGCGGCTCCGCCGCGCCCTGGTTCTGATTCCGAATCCGCGCC
CTGACCCCTGAATCCTGAAC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSIPADLKYTQSHEWVRAEADGSVTVGITHHAQDLLGDMVFIENPAVGRTLAKGEECAVVESVKAASDVYAPVAGEVIAA
NGEVESSPESVNQDAYTAWLFKIKPANPADVDALLDAAAYQKVVESEAH

Sequences:

>Translated_129_residues
MSIPADLKYTQSHEWVRAEADGSVTVGITHHAQDLLGDMVFIENPAVGRTLAKGEECAVVESVKAASDVYAPVAGEVIAA
NGEVESSPESVNQDAYTAWLFKIKPANPADVDALLDAAAYQKVVESEAH
>Mature_128_residues
SIPADLKYTQSHEWVRAEADGSVTVGITHHAQDLLGDMVFIENPAVGRTLAKGEECAVVESVKAASDVYAPVAGEVIAAN
GEVESSPESVNQDAYTAWLFKIKPANPADVDALLDAAAYQKVVESEAH

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=43.3333333333333, Blast_Score=102, Evalue=6e-23,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=42.5, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=55.9055118110236, Blast_Score=157, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=42.5, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17551294, Length=121, Percent_Identity=40.495867768595, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=37.6, Blast_Score=91, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=44.1666666666667, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_THIDA (Q3SMB7)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_313935.1
- ProteinModelPortal:   Q3SMB7
- SMR:   Q3SMB7
- STRING:   Q3SMB7
- GeneID:   3673623
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0177
- NMPDR:   fig|292415.3.peg.126
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- PhylomeDB:   Q3SMB7
- ProtClustDB:   PRK01202
- BioCyc:   TDEN292415:TBD_0177-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13666; Mature: 13535

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPADLKYTQSHEWVRAEADGSVTVGITHHAQDLLGDMVFIENPAVGRTLAKGEECAVV
CCCCCCCEECCCCCEEEECCCCCEEEEEEHHHHHHHHCEEEECCCCCCCHHCCCCCCHHH
ESVKAASDVYAPVAGEVIAANGEVESSPESVNQDAYTAWLFKIKPANPADVDALLDAAAY
HHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
QKVVESEAH
HHHHHHCCC
>Mature Secondary Structure 
SIPADLKYTQSHEWVRAEADGSVTVGITHHAQDLLGDMVFIENPAVGRTLAKGEECAVV
CCCCCCEECCCCCEEEECCCCCEEEEEEHHHHHHHHCEEEECCCCCCCHHCCCCCCHHH
ESVKAASDVYAPVAGEVIAANGEVESSPESVNQDAYTAWLFKIKPANPADVDALLDAAAY
HHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
QKVVESEAH
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA