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The map label for this gene is groL

Identifier: 74316109

GI number: 74316109

Start: 96510

End: 98153

Strand: Reverse

Name: groL

Synonym: Tbd_0091

Alternate gene names: 74316109

Gene position: 98153-96510 (Counterclockwise)

Preceding gene: 74316110

Following gene: 74316108

Centisome position: 3.37

GC content: 64.36

Gene sequence:

>1644_bases
ATGGCTGCAAAAGACGTACGTTTTGGTGATTCCGCGCGTCACCGCATGGTGGCGGGTGTCAACGTTCTGGCTGACGCCGT
CAAGGTGACGCTGGGCCCGAAAGGCCGCAACGTGGTACTCGACCGTTCTTTCGGCGCCCCGACGGTGACCAAGGACGGCG
TGTCGGTCGCCAAGGAAATCGAGCTCAAGGACAAGCTCGAGAACATGGGCGCGCAGATGGTCAAGGAAGTCGCGTCGAAG
ACCTCCGACGTCGCCGGTGACGGGACCACGACCGCGACCGTGCTCGCCCAGTCGATCGTCAACGAAGGCATGAAGTTCGT
CGCCGCGGGCATGAACCCGATGGACCTCAAGCGTGGCATCGACAAGGCGGTCAACGCGATCACCGAAGAACTGAAGAAGA
TCTCGAAGCCCTGCACGACGAGCAAGGAAATCGCCCAGGTCGGCTCGATCTCGGCCAACTCCGACGCCCCGATCGGCCAG
ATCATCGCCGACGCGATGGACAAGGTCGGCAAGGAAGGCGTGATCACGGTCGAAGACGGCTCCGGGCTGGAAAACGAGCT
CGACGTCGTCGAGGGCATGCAGTTCGACCGCGGCTACCTGTCGCCCTACTTCATCAACAACGCCGACAAGCAGATGGCGA
TCATGGACGATCCCTTCATCCTCTTGTTCGACAAGAAGATCTCCAACATCCGTGACCTGCTGCCCGTGCTCGAGCAGGTC
GCCAAGGCCGGCAAGCCGCTGATGATCGTCGCCGAAGACGTCGACGGCGAAGCGCTCGCCACGCTCGTCGTCAACAACAT
CCGCGGCATCCTCAAGACCTGCGCGGTCAAGGCGCCGGGCTTCGGCGACCGTCGCAAGGCGATGCTCGAAGACATGGCCA
TCCTGACCGGCGGCACCGTGATCGCCGAGGAAGTCGGCTTGAGCCTCGAGAAGGCCACCCTGCAGGATCTGGGCCGCGCC
AAGCGCATCGAGGTCGGCAAGGAGAACACCACGATCATCGACGGCGCCGGTGACGGCAACGCGATCAAGGGCCGCATCGC
CCAGATCAACAAGCAGATCGAGGAAGTCACCTCCGACTACGACAAGGAAAAGCTGCAGGAGCGCAAGGCCAAGCTGGCCG
GTGGCGTCGCCGTGATCAAGGTCGGCGCTGCGACCGAAGTCGAGATGAAGGAGAAGAAGGCCCGCGTCGAAGACGCGCTG
CACGCGACCCGTGCCGCGGTCGAAGAAGGCATCGTTCCCGGCGGCGGCGTGGCACTGCTGCGCGCCAAGGCCGCGGCTGC
CGCGATCAAGGGCGACAACCACGACCAGGATGCGGGCGTGAAGATCGTGCTGCGCGCGATCGAGGAGCCGCTGCGCCAGA
TCGTCAAGAACTGCGGCGACGAGCCGTCGGTCGTCGTCAACAAGGTGCTCGAAGGCGAGGGCAACTTCGGCTACAACGCC
GGCACCAGCGAGTACGGCGACATGGTCGCGATGGGCGTGCTCGACCCGACCAAGGTCACGCGCACCGCGCTGCAGAACGC
CGCATCGATCGCCGGCCTGATGCTGACTACCGACTGCATGGTCGCCGACCTGCCGGAAGACAAGGCGCCCGCGATGCCGA
TGGGTGGCGGCATGGGCGACATGGGCGGCATGGGCATGATGTAA

Upstream 100 bases:

>100_bases
GCTCTTGGTGATGCGCGAAGAAGACATCATGGGCGTGGTCGAGCAGTAAGCCGCCACCGCACGCAAACCCAACCGAATTC
AAATATCTAGGAGTTAAGCA

Downstream 100 bases:

>100_bases
GCCCGTCGTTCGACCGCGGCCGGCTCGAGATGTCCGTCAGGGCACCTGCGGGTAACGAACAGCACCCTGTTCGGCCGTTC
AAAAGCCCCGCCTCGGCGGG

Product: chaperonin GroEL (Hsp60, Cpn10)

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MAAKDVRFGDSARHRMVAGVNVLADAVKVTLGPKGRNVVLDRSFGAPTVTKDGVSVAKEIELKDKLENMGAQMVKEVASK
TSDVAGDGTTTATVLAQSIVNEGMKFVAAGMNPMDLKRGIDKAVNAITEELKKISKPCTTSKEIAQVGSISANSDAPIGQ
IIADAMDKVGKEGVITVEDGSGLENELDVVEGMQFDRGYLSPYFINNADKQMAIMDDPFILLFDKKISNIRDLLPVLEQV
AKAGKPLMIVAEDVDGEALATLVVNNIRGILKTCAVKAPGFGDRRKAMLEDMAILTGGTVIAEEVGLSLEKATLQDLGRA
KRIEVGKENTTIIDGAGDGNAIKGRIAQINKQIEEVTSDYDKEKLQERKAKLAGGVAVIKVGAATEVEMKEKKARVEDAL
HATRAAVEEGIVPGGGVALLRAKAAAAAIKGDNHDQDAGVKIVLRAIEEPLRQIVKNCGDEPSVVVNKVLEGEGNFGYNA
GTSEYGDMVAMGVLDPTKVTRTALQNAASIAGLMLTTDCMVADLPEDKAPAMPMGGGMGDMGGMGMM

Sequences:

>Translated_547_residues
MAAKDVRFGDSARHRMVAGVNVLADAVKVTLGPKGRNVVLDRSFGAPTVTKDGVSVAKEIELKDKLENMGAQMVKEVASK
TSDVAGDGTTTATVLAQSIVNEGMKFVAAGMNPMDLKRGIDKAVNAITEELKKISKPCTTSKEIAQVGSISANSDAPIGQ
IIADAMDKVGKEGVITVEDGSGLENELDVVEGMQFDRGYLSPYFINNADKQMAIMDDPFILLFDKKISNIRDLLPVLEQV
AKAGKPLMIVAEDVDGEALATLVVNNIRGILKTCAVKAPGFGDRRKAMLEDMAILTGGTVIAEEVGLSLEKATLQDLGRA
KRIEVGKENTTIIDGAGDGNAIKGRIAQINKQIEEVTSDYDKEKLQERKAKLAGGVAVIKVGAATEVEMKEKKARVEDAL
HATRAAVEEGIVPGGGVALLRAKAAAAAIKGDNHDQDAGVKIVLRAIEEPLRQIVKNCGDEPSVVVNKVLEGEGNFGYNA
GTSEYGDMVAMGVLDPTKVTRTALQNAASIAGLMLTTDCMVADLPEDKAPAMPMGGGMGDMGGMGMM
>Mature_546_residues
AAKDVRFGDSARHRMVAGVNVLADAVKVTLGPKGRNVVLDRSFGAPTVTKDGVSVAKEIELKDKLENMGAQMVKEVASKT
SDVAGDGTTTATVLAQSIVNEGMKFVAAGMNPMDLKRGIDKAVNAITEELKKISKPCTTSKEIAQVGSISANSDAPIGQI
IADAMDKVGKEGVITVEDGSGLENELDVVEGMQFDRGYLSPYFINNADKQMAIMDDPFILLFDKKISNIRDLLPVLEQVA
KAGKPLMIVAEDVDGEALATLVVNNIRGILKTCAVKAPGFGDRRKAMLEDMAILTGGTVIAEEVGLSLEKATLQDLGRAK
RIEVGKENTTIIDGAGDGNAIKGRIAQINKQIEEVTSDYDKEKLQERKAKLAGGVAVIKVGAATEVEMKEKKARVEDALH
ATRAAVEEGIVPGGGVALLRAKAAAAAIKGDNHDQDAGVKIVLRAIEEPLRQIVKNCGDEPSVVVNKVLEGEGNFGYNAG
TSEYGDMVAMGVLDPTKVTRTALQNAASIAGLMLTTDCMVADLPEDKAPAMPMGGGMGDMGGMGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=530, Percent_Identity=53.0188679245283, Blast_Score=538, Evalue=1e-153,
Organism=Homo sapiens, GI31542947, Length=530, Percent_Identity=53.0188679245283, Blast_Score=538, Evalue=1e-153,
Organism=Escherichia coli, GI1790586, Length=529, Percent_Identity=75.2362948960302, Blast_Score=811, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=530, Percent_Identity=52.2641509433962, Blast_Score=543, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI193210679, Length=212, Percent_Identity=51.4150943396226, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17564182, Length=558, Percent_Identity=24.3727598566308, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=53.4220532319392, Blast_Score=550, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6322524, Length=398, Percent_Identity=23.1155778894472, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24641193, Length=526, Percent_Identity=51.5209125475285, Blast_Score=538, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24641191, Length=526, Percent_Identity=51.5209125475285, Blast_Score=538, Evalue=1e-153,
Organism=Drosophila melanogaster, GI45550936, Length=524, Percent_Identity=49.4274809160305, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45550132, Length=524, Percent_Identity=49.4274809160305, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45550935, Length=524, Percent_Identity=49.4274809160305, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI17864606, Length=553, Percent_Identity=42.6763110307414, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24584129, Length=534, Percent_Identity=35.5805243445693, Blast_Score=333, Evalue=3e-91,
Organism=Drosophila melanogaster, GI19921262, Length=534, Percent_Identity=35.5805243445693, Blast_Score=333, Evalue=3e-91,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_THIDA (Q3SMK1)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_313849.1
- HSSP:   P0A6F5
- ProteinModelPortal:   Q3SMK1
- SMR:   Q3SMK1
- STRING:   Q3SMK1
- GeneID:   3673869
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0091
- NMPDR:   fig|292415.3.peg.991
- eggNOG:   COG0459
- HOGENOM:   HBG625289
- OMA:   NSDTSIG
- PhylomeDB:   Q3SMK1
- BioCyc:   TDEN292415:TBD_0091-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57563; Mature: 57432

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKDVRFGDSARHRMVAGVNVLADAVKVTLGPKGRNVVLDRSFGAPTVTKDGVSVAKEI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCCCHHHHHHH
ELKDKLENMGAQMVKEVASKTSDVAGDGTTTATVLAQSIVNEGMKFVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHH
DKAVNAITEELKKISKPCTTSKEIAQVGSISANSDAPIGQIIADAMDKVGKEGVITVEDG
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECC
SGLENELDVVEGMQFDRGYLSPYFINNADKQMAIMDDPFILLFDKKISNIRDLLPVLEQV
CCCCHHHHHHHCCCCCCCCCCCCEECCCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHH
AKAGKPLMIVAEDVDGEALATLVVNNIRGILKTCAVKAPGFGDRRKAMLEDMAILTGGTV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHH
IAEEVGLSLEKATLQDLGRAKRIEVGKENTTIIDGAGDGNAIKGRIAQINKQIEEVTSDY
HHHHHCCCHHHHHHHHCCCHHEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DKEKLQERKAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RAKAAAAAIKGDNHDQDAGVKIVLRAIEEPLRQIVKNCGDEPSVVVNKVLEGEGNFGYNA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
GTSEYGDMVAMGVLDPTKVTRTALQNAASIAGLMLTTDCMVADLPEDKAPAMPMGGGMGD
CCCHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCC
MGGMGMM
CCCCCCC
>Mature Secondary Structure 
AAKDVRFGDSARHRMVAGVNVLADAVKVTLGPKGRNVVLDRSFGAPTVTKDGVSVAKEI
CCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCCCHHHHHHH
ELKDKLENMGAQMVKEVASKTSDVAGDGTTTATVLAQSIVNEGMKFVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHH
DKAVNAITEELKKISKPCTTSKEIAQVGSISANSDAPIGQIIADAMDKVGKEGVITVEDG
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECC
SGLENELDVVEGMQFDRGYLSPYFINNADKQMAIMDDPFILLFDKKISNIRDLLPVLEQV
CCCCHHHHHHHCCCCCCCCCCCCEECCCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHH
AKAGKPLMIVAEDVDGEALATLVVNNIRGILKTCAVKAPGFGDRRKAMLEDMAILTGGTV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHH
IAEEVGLSLEKATLQDLGRAKRIEVGKENTTIIDGAGDGNAIKGRIAQINKQIEEVTSDY
HHHHHCCCHHHHHHHHCCCHHEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DKEKLQERKAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RAKAAAAAIKGDNHDQDAGVKIVLRAIEEPLRQIVKNCGDEPSVVVNKVLEGEGNFGYNA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
GTSEYGDMVAMGVLDPTKVTRTALQNAASIAGLMLTTDCMVADLPEDKAPAMPMGGGMGD
CCCHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCC
MGGMGMM
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA