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The map label for this gene is topA [H]

Identifier: 74316028

GI number: 74316028

Start: 8962

End: 11535

Strand: Reverse

Name: topA [H]

Synonym: Tbd_0010

Alternate gene names: 74316028

Gene position: 11535-8962 (Counterclockwise)

Preceding gene: 74316029

Following gene: 74316027

Centisome position: 0.4

GC content: 66.12

Gene sequence:

>2574_bases
ATGTCCAAGCTCGTCATCGTCGAATCGCCGTCGAAGTCCAAGTCGCTGAAGAAATACCTCGGCGCGGACTACGACATCCT
CGCCAGCTACGGCCACGTGCGCGACCTCGAGCCGAGGACCGGCGCGGTCGACACGGAAAACTTCTCGATGAAGTACCAGA
TCATCGAGCGCAACGCCAAGCACGTCGACGCCATCGTCAAGGCCGCGAAGAAAGCCGACGAAGTCCTGCTCGCGACCGAC
CCGGACCGCGAAGGCGAGGCGATTTCCTGGCACATCAGCGAAATCCTCAAGGAGAAGAAGGTCAAGACGCCGATGAAGCG
CGTCGCCTTCTACGAGATCACCGAATCGGCGATCCAGGACGCGGTGCGCAATCCGCGCGAGATCGCGTCCGATCTCGTCG
ACGCCCAGCAGGCGCGGCGCGCACTCGATTACCTGGTCGGCTTCAACCTCTCGCCGCTCTTGTGGAAAAAGATCAGCCCC
GGGCTGTCGGCCGGCCGCGTGCAGTCGCCCGCGCTCCGCATGATCGTCGAGCGCGAGGAGGAGATCGAGGCCTTCGTCGC
GCGCGAATACTGGACGCTGCACCTCGACTCACATAAAGGCAGTCAAGCGTTCACGGCCAAGCTCACGCACTTCAAGGGCG
AGAAGCTCGACCAGTTCGCGGTCGAGCACGAGGCGCGCAGCAAGGAATGGCTCGCCACGCTTGAATCCAAGGATGCGCGC
GTGATGCGCATCGAGAAGAAGCGCCGCGCCCGCAACCCCGCCGCGCCGTTCACGACCTCGACGCTGCAGCAGGCCGGCGT
GCGTCAGCTCGGTATGACGACCGACCGCGTGATGCGCACCGCGCAGCAACTCTACGAGGGCATCGACCTCGGCGAAGGCC
CGGTCGGCCTCATCACCTACATGCGGACCGACTCGGTCAACCTCGCGCAGGAAGCGATCACCGACATCCGCAAGTATGTG
GGCGCCAACTTCGACAAGGATTACCTGCCGCCGCACGCGGTCCAGTACAAGGCCAAGACCAAGAACGCGCAGGAAGCGCA
CGAGGCCGTGCGCCCGACCTCGGTGTTGCGCACGCCCGACGCGGTCAGGCAATTCCTGACCCAGGACCAGGCCCGGCTTT
ACGAACTCGTCTGGAAGCGCACGGTCGCCTGCCAGATGACGGCCGCGCAGTTCGACACGGTCGCTGCCGACATCACGGTC
GGCGAGGGCACCTTCCGCGCGACAGGGCAGACACTCGCGTTCGCCGGCTTTCTCGCGGTCTACCAGGACGACGAGGACGA
CGAAGGCGAGTCGAAGCTGCCCGCGCTCGTCGAGGGGGAAACCCTGCCCGTCGACCGGCTCTACGGCGAACAGCACTTCA
CCCAGCCGCCGCCGCGCTATACGGAAGCGTCCCTGGTCAAGGCGCTCGAGGAATACGGCATCGGCCGGCCGTCGACCTAC
GCCAGCATCATCTCGACGCTGCAGGACCGCGAATACGTCGTGCTCGAAAAGAAGCGCTTCCAGCCGACCGACGTCGGCCG
ACTGGTCAACTGCTTCCTGACCCGGCATTTCAGCCACTACGTCGACTACGACTTCACCGCCAAGCTCGAGGACAAGCTCG
ACGACGTCTCCAACGGCAAGCGCGTGTGGTCGCGTCTGCTGGGCGAATTCTGGAGCGGCTTCGAAGGCGACCTCAAGGCC
AAGCAGGACGTCGAGCGCGGCTGCCCGATCCTCGAAGCCTGCCCCAAGTGCGCCAAGCCGCTCTTCATGCAAGCGAGCAA
GCGCGGGCTCTTCATCGGCTGCTCGGGCTATCCCGAATGCGACTACACGCGGCCGCTGCACCCCGCCACCGCCGAAGGCG
ACAACATCCTCGGCAAGGACCCGGCGAGCGGGCTCGACGTCAAGCTGCTCTCCGGCCGCTTCGGCCCTTACGTGCAGATC
GGCGATATGCCCGAGGACAAGAAGGCGCCGAAGCCGCGGCGCGCGTCGTGGCCCAAGGACATTCCGCTCGAAAACGCGAC
GCTCGACCTCGCGCTCAAATTCCTCTCGCTGCCGCGCGAGGTCGGGCATCACCCGACCACCGGCCTGCCGATCGTCGCCA
ACAACGGCCGCTTCGGCCCGTATCTGCTGCATGACGGCAAGTTCAAGTCGATCCCGAAGACCGACAGCGTCTACGAGATC
GACCTGCCGCGCGCGCTCGAGGTGCTGGCGATGGAGCGCGCGCCGCGCGGCGCCGACTCGGCCGCGTCGGTGCTGAAGAA
TCTCGGCCCGCACCCGGAAGACGGCAAGGACATCACGGTGCGCTCGGGCCGCTACGGGCCCTACGTCAAGCACGGCAGCA
CCAACGCGACGCTGCCGAAGGACATCACGCCCGAAGCGGTCACGCTCGACGAGGCGATCGGCCTGATCGCCGCGCGCGTC
GCCAAGGGGCCGGCGAAGAAGCCGCCGCGCAAGGCGCCGACCAAGGCGAGCGCGACGAAGACTAAGGCCGCCGACACCGC
GACCAAGCCCGCAGCGACTAAAAAGGCGGCGACGAAGAAAGCGCCCGCCAAGCCCGCAGCGACGAAAAAGCCCGCCAGCA
AAAAAGCCGCCTAG

Upstream 100 bases:

>100_bases
GACGCTGATCGTGCTGTGGAGCCGCGGGCTGATCGAACATTTCACCCACCTCGAAGAGATGCTGCTGAACCATGAGCCGG
GACGCCTGCACTAAGCCGAC

Downstream 100 bases:

>100_bases
CCGTGCCGTCCAGCCTGCTCATGGTCAACGCCTTCATCGGCACGGCCGGCTACAAGAAGCTCTATCAGGAACTCGGTTTC
TCGGTCACGACCGAATGGTC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 857; Mature: 856

Protein sequence:

>857_residues
MSKLVIVESPSKSKSLKKYLGADYDILASYGHVRDLEPRTGAVDTENFSMKYQIIERNAKHVDAIVKAAKKADEVLLATD
PDREGEAISWHISEILKEKKVKTPMKRVAFYEITESAIQDAVRNPREIASDLVDAQQARRALDYLVGFNLSPLLWKKISP
GLSAGRVQSPALRMIVEREEEIEAFVAREYWTLHLDSHKGSQAFTAKLTHFKGEKLDQFAVEHEARSKEWLATLESKDAR
VMRIEKKRRARNPAAPFTTSTLQQAGVRQLGMTTDRVMRTAQQLYEGIDLGEGPVGLITYMRTDSVNLAQEAITDIRKYV
GANFDKDYLPPHAVQYKAKTKNAQEAHEAVRPTSVLRTPDAVRQFLTQDQARLYELVWKRTVACQMTAAQFDTVAADITV
GEGTFRATGQTLAFAGFLAVYQDDEDDEGESKLPALVEGETLPVDRLYGEQHFTQPPPRYTEASLVKALEEYGIGRPSTY
ASIISTLQDREYVVLEKKRFQPTDVGRLVNCFLTRHFSHYVDYDFTAKLEDKLDDVSNGKRVWSRLLGEFWSGFEGDLKA
KQDVERGCPILEACPKCAKPLFMQASKRGLFIGCSGYPECDYTRPLHPATAEGDNILGKDPASGLDVKLLSGRFGPYVQI
GDMPEDKKAPKPRRASWPKDIPLENATLDLALKFLSLPREVGHHPTTGLPIVANNGRFGPYLLHDGKFKSIPKTDSVYEI
DLPRALEVLAMERAPRGADSAASVLKNLGPHPEDGKDITVRSGRYGPYVKHGSTNATLPKDITPEAVTLDEAIGLIAARV
AKGPAKKPPRKAPTKASATKTKAADTATKPAATKKAATKKAPAKPAATKKPASKKAA

Sequences:

>Translated_857_residues
MSKLVIVESPSKSKSLKKYLGADYDILASYGHVRDLEPRTGAVDTENFSMKYQIIERNAKHVDAIVKAAKKADEVLLATD
PDREGEAISWHISEILKEKKVKTPMKRVAFYEITESAIQDAVRNPREIASDLVDAQQARRALDYLVGFNLSPLLWKKISP
GLSAGRVQSPALRMIVEREEEIEAFVAREYWTLHLDSHKGSQAFTAKLTHFKGEKLDQFAVEHEARSKEWLATLESKDAR
VMRIEKKRRARNPAAPFTTSTLQQAGVRQLGMTTDRVMRTAQQLYEGIDLGEGPVGLITYMRTDSVNLAQEAITDIRKYV
GANFDKDYLPPHAVQYKAKTKNAQEAHEAVRPTSVLRTPDAVRQFLTQDQARLYELVWKRTVACQMTAAQFDTVAADITV
GEGTFRATGQTLAFAGFLAVYQDDEDDEGESKLPALVEGETLPVDRLYGEQHFTQPPPRYTEASLVKALEEYGIGRPSTY
ASIISTLQDREYVVLEKKRFQPTDVGRLVNCFLTRHFSHYVDYDFTAKLEDKLDDVSNGKRVWSRLLGEFWSGFEGDLKA
KQDVERGCPILEACPKCAKPLFMQASKRGLFIGCSGYPECDYTRPLHPATAEGDNILGKDPASGLDVKLLSGRFGPYVQI
GDMPEDKKAPKPRRASWPKDIPLENATLDLALKFLSLPREVGHHPTTGLPIVANNGRFGPYLLHDGKFKSIPKTDSVYEI
DLPRALEVLAMERAPRGADSAASVLKNLGPHPEDGKDITVRSGRYGPYVKHGSTNATLPKDITPEAVTLDEAIGLIAARV
AKGPAKKPPRKAPTKASATKTKAADTATKPAATKKAATKKAPAKPAATKKPASKKAA
>Mature_856_residues
SKLVIVESPSKSKSLKKYLGADYDILASYGHVRDLEPRTGAVDTENFSMKYQIIERNAKHVDAIVKAAKKADEVLLATDP
DREGEAISWHISEILKEKKVKTPMKRVAFYEITESAIQDAVRNPREIASDLVDAQQARRALDYLVGFNLSPLLWKKISPG
LSAGRVQSPALRMIVEREEEIEAFVAREYWTLHLDSHKGSQAFTAKLTHFKGEKLDQFAVEHEARSKEWLATLESKDARV
MRIEKKRRARNPAAPFTTSTLQQAGVRQLGMTTDRVMRTAQQLYEGIDLGEGPVGLITYMRTDSVNLAQEAITDIRKYVG
ANFDKDYLPPHAVQYKAKTKNAQEAHEAVRPTSVLRTPDAVRQFLTQDQARLYELVWKRTVACQMTAAQFDTVAADITVG
EGTFRATGQTLAFAGFLAVYQDDEDDEGESKLPALVEGETLPVDRLYGEQHFTQPPPRYTEASLVKALEEYGIGRPSTYA
SIISTLQDREYVVLEKKRFQPTDVGRLVNCFLTRHFSHYVDYDFTAKLEDKLDDVSNGKRVWSRLLGEFWSGFEGDLKAK
QDVERGCPILEACPKCAKPLFMQASKRGLFIGCSGYPECDYTRPLHPATAEGDNILGKDPASGLDVKLLSGRFGPYVQIG
DMPEDKKAPKPRRASWPKDIPLENATLDLALKFLSLPREVGHHPTTGLPIVANNGRFGPYLLHDGKFKSIPKTDSVYEID
LPRALEVLAMERAPRGADSAASVLKNLGPHPEDGKDITVRSGRYGPYVKHGSTNATLPKDITPEAVTLDEAIGLIAARVA
KGPAKKPPRKAPTKASATKTKAADTATKPAATKKAATKKAPAKPAATKKPASKKAA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=606, Percent_Identity=26.4026402640264, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI4507635, Length=506, Percent_Identity=23.5177865612648, Blast_Score=109, Evalue=1e-23,
Organism=Escherichia coli, GI1787529, Length=663, Percent_Identity=40.7239819004525, Blast_Score=460, Evalue=1e-130,
Organism=Escherichia coli, GI1788061, Length=503, Percent_Identity=25.844930417495, Blast_Score=124, Evalue=4e-29,
Organism=Escherichia coli, GI48994931, Length=78, Percent_Identity=41.025641025641, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555378, Length=502, Percent_Identity=26.6932270916335, Blast_Score=144, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32563869, Length=553, Percent_Identity=25.3164556962025, Blast_Score=112, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=518, Percent_Identity=24.7104247104247, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585251, Length=674, Percent_Identity=24.0356083086053, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24640096, Length=459, Percent_Identity=25.4901960784314, Blast_Score=98, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 94823; Mature: 94692

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVIVESPSKSKSLKKYLGADYDILASYGHVRDLEPRTGAVDTENFSMKYQIIERNAK
CCCEEEEECCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCHH
HVDAIVKAAKKADEVLLATDPDREGEAISWHISEILKEKKVKTPMKRVAFYEITESAIQD
HHHHHHHHHHHHCCEEEECCCCCCCCEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
AVRNPREIASDLVDAQQARRALDYLVGFNLSPLLWKKISPGLSAGRVQSPALRMIVEREE
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EIEAFVAREYWTLHLDSHKGSQAFTAKLTHFKGEKLDQFAVEHEARSKEWLATLESKDAR
HHHHHHHHCEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
VMRIEKKRRARNPAAPFTTSTLQQAGVRQLGMTTDRVMRTAQQLYEGIDLGEGPVGLITY
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEE
MRTDSVNLAQEAITDIRKYVGANFDKDYLPPHAVQYKAKTKNAQEAHEAVRPTSVLRTPD
EECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHCCCHHHHHHHHCCHHHHCCHH
AVRQFLTQDQARLYELVWKRTVACQMTAAQFDTVAADITVGEGTFRATGQTLAFAGFLAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEECCCCHHHHHHHHHE
YQDDEDDEGESKLPALVEGETLPVDRLYGEQHFTQPPPRYTEASLVKALEEYGIGRPSTY
ECCCCCCCCCCCCCEEECCCCCCHHHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCCCHHH
ASIISTLQDREYVVLEKKRFQPTDVGRLVNCFLTRHFSHYVDYDFTAKLEDKLDDVSNGK
HHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCHH
RVWSRLLGEFWSGFEGDLKAKQDVERGCPILEACPKCAKPLFMQASKRGLFIGCSGYPEC
HHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHCHHHHHHHHHHHCCCCEEEECCCCCCC
DYTRPLHPATAEGDNILGKDPASGLDVKLLSGRFGPYVQIGDMPEDKKAPKPRRASWPKD
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCC
IPLENATLDLALKFLSLPREVGHHPTTGLPIVANNGRFGPYLLHDGKFKSIPKTDSVYEI
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEECCCCCCCCCCCCEEEE
DLPRALEVLAMERAPRGADSAASVLKNLGPHPEDGKDITVRSGRYGPYVKHGSTNATLPK
CCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCEECCCCCCCCCC
DITPEAVTLDEAIGLIAARVAKGPAKKPPRKAPTKASATKTKAADTATKPAATKKAATKK
CCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHCC
APAKPAATKKPASKKAA
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKLVIVESPSKSKSLKKYLGADYDILASYGHVRDLEPRTGAVDTENFSMKYQIIERNAK
CCEEEEECCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCHH
HVDAIVKAAKKADEVLLATDPDREGEAISWHISEILKEKKVKTPMKRVAFYEITESAIQD
HHHHHHHHHHHHCCEEEECCCCCCCCEEEHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
AVRNPREIASDLVDAQQARRALDYLVGFNLSPLLWKKISPGLSAGRVQSPALRMIVEREE
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EIEAFVAREYWTLHLDSHKGSQAFTAKLTHFKGEKLDQFAVEHEARSKEWLATLESKDAR
HHHHHHHHCEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
VMRIEKKRRARNPAAPFTTSTLQQAGVRQLGMTTDRVMRTAQQLYEGIDLGEGPVGLITY
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEE
MRTDSVNLAQEAITDIRKYVGANFDKDYLPPHAVQYKAKTKNAQEAHEAVRPTSVLRTPD
EECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHCCCHHHHHHHHCCHHHHCCHH
AVRQFLTQDQARLYELVWKRTVACQMTAAQFDTVAADITVGEGTFRATGQTLAFAGFLAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEECCCCHHHHHHHHHE
YQDDEDDEGESKLPALVEGETLPVDRLYGEQHFTQPPPRYTEASLVKALEEYGIGRPSTY
ECCCCCCCCCCCCCEEECCCCCCHHHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCCCHHH
ASIISTLQDREYVVLEKKRFQPTDVGRLVNCFLTRHFSHYVDYDFTAKLEDKLDDVSNGK
HHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCHH
RVWSRLLGEFWSGFEGDLKAKQDVERGCPILEACPKCAKPLFMQASKRGLFIGCSGYPEC
HHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHCHHHHHHHHHHHCCCCEEEECCCCCCC
DYTRPLHPATAEGDNILGKDPASGLDVKLLSGRFGPYVQIGDMPEDKKAPKPRRASWPKD
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCC
IPLENATLDLALKFLSLPREVGHHPTTGLPIVANNGRFGPYLLHDGKFKSIPKTDSVYEI
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEECCCCCCCCCCCCEEEE
DLPRALEVLAMERAPRGADSAASVLKNLGPHPEDGKDITVRSGRYGPYVKHGSTNATLPK
CCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCEECCCCCCCCCC
DITPEAVTLDEAIGLIAARVAKGPAKKPPRKAPTKASATKTKAADTATKPAATKKAATKK
CCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHCC
APAKPAATKKPASKKAA
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]