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The map label for this gene is argG

Identifier: 161899032

GI number: 161899032

Start: 1942816

End: 1944045

Strand: Reverse

Name: argG

Synonym: Tbd_1849

Alternate gene names: 161899032

Gene position: 1944045-1942816 (Counterclockwise)

Preceding gene: 74317868

Following gene: 74317866

Centisome position: 66.81

GC content: 63.01

Gene sequence:

>1230_bases
ATGAGCGACATCAAGAAAGTTGTACTGGCCTATTCCGGCGGCCTCGACACCTCGGTCATCCTGAAATGGCTGCAGGACAC
CTACCAATGCGAGGTGGTGACCTTCACCGCCGACCTCGGCCAGGGCGAGGAACTCGAACCGGCGCGGCAGAAGGCACTGC
AGTTCGGCATCAAACCTGAACAGATCTACATCGACGACCTGCGCGAGGAATTCGTGCGCGACTTCGTCTTCCCGATGTTC
CGCGCGAATACCGTGTACGAAGGCGAATATCTGCTCGGCACGTCGATCGCGCGGCCGCTGATCGCCAAGCGGCTGATCGA
GATCGTCAACGCAACCGGCGCCGACGCGATCTGTCACGGCGCCACCGGTAAGGGCAACGACCAGGTGCGCTTCGAACTCG
GCGCCTACGCGCTGAAACCCGACGTCAAGGTCATCGCGCCGTGGCGCGAGTGGGACCTGCTCTCGCGCGAGAAGCTGCTC
GCCTACGCAGAATCCCATGGCATTCCGATCGACATGAAGCATCGCCAGGGCGGCTCGCCTTATTCGATGGACGCGAACCT
GCTGCATATTTCCTACGAAGGCCGCCACCTCGAGGACCCCAGCGCCGAAGCCGAAGAAGACATGTGGCGCTGGACCGTGT
CGCCCGAGAAGGCGCCCGACGCGGCCGAATACATCGAACTGACCTACGCGAAAGGTGATGTCGTCGCGATCGACGGCCAG
CAAATGCCTGCCCACGAAGTGCTCGCCAAGCTCAACGAACTGGGCGGCAAGCACGGCATCGGCCGGCTCGACCTCGTCGA
GAACCGCTACGTCGGCATGAAGTCGCGGGGCTGCTACGAAACGCCCGGCGGCACCATCCTGCTGAAGGCGCACCGTGCGA
TCGAGTCGATCACGCTCGATCGCGAGGTCGCGCATCTGAAGGACGACCTGATGCCGCGCTACGCCAGCCTGATCTACAAC
GGCTACTGGTGGGCGCCCGAGCGGCGCGCGCTGCAGGTGCTGATCGACCACACGCAGGCGCACGTCAACGGCACGGTGCG
GCTCAAGCTCTACAAGGGCAACGTCATCGTCGTCGGCCGCGATTCGAAGAACGATTCGCTGTTCGACTCGACCATCGCAA
CCTTCGAGGACGACGCGGGCGCCTACGATCAGAAGGACGCCGGCGGTTTCATCAAGCTCAACGCGCTGCGCATGCGCATC
GCCGCAAAACTCGACGCACGGAGAAAATAA

Upstream 100 bases:

>100_bases
GCCGCCAATCTCGACATTCTCAAGATCGGCGGCTTCCCGGGCCAGATCGAGATCATCGACACGAACACTTCCGGCTCCAC
CCATTAATCCAGCGACTCCC

Downstream 100 bases:

>100_bases
GCATGTCCCGGTTCGACAACGTCGCAGTCACCAAGCAGGCCAACGTCTATTTCGACGGCAAGTGCGTTTCGCATACCGTC
GAATTCGCCGACGGCACCAA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSDIKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEELEPARQKALQFGIKPEQIYIDDLREEFVRDFVFPMF
RANTVYEGEYLLGTSIARPLIAKRLIEIVNATGADAICHGATGKGNDQVRFELGAYALKPDVKVIAPWREWDLLSREKLL
AYAESHGIPIDMKHRQGGSPYSMDANLLHISYEGRHLEDPSAEAEEDMWRWTVSPEKAPDAAEYIELTYAKGDVVAIDGQ
QMPAHEVLAKLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTILLKAHRAIESITLDREVAHLKDDLMPRYASLIYN
GYWWAPERRALQVLIDHTQAHVNGTVRLKLYKGNVIVVGRDSKNDSLFDSTIATFEDDAGAYDQKDAGGFIKLNALRMRI
AAKLDARRK

Sequences:

>Translated_409_residues
MSDIKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEELEPARQKALQFGIKPEQIYIDDLREEFVRDFVFPMF
RANTVYEGEYLLGTSIARPLIAKRLIEIVNATGADAICHGATGKGNDQVRFELGAYALKPDVKVIAPWREWDLLSREKLL
AYAESHGIPIDMKHRQGGSPYSMDANLLHISYEGRHLEDPSAEAEEDMWRWTVSPEKAPDAAEYIELTYAKGDVVAIDGQ
QMPAHEVLAKLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTILLKAHRAIESITLDREVAHLKDDLMPRYASLIYN
GYWWAPERRALQVLIDHTQAHVNGTVRLKLYKGNVIVVGRDSKNDSLFDSTIATFEDDAGAYDQKDAGGFIKLNALRMRI
AAKLDARRK
>Mature_408_residues
SDIKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEELEPARQKALQFGIKPEQIYIDDLREEFVRDFVFPMFR
ANTVYEGEYLLGTSIARPLIAKRLIEIVNATGADAICHGATGKGNDQVRFELGAYALKPDVKVIAPWREWDLLSREKLLA
YAESHGIPIDMKHRQGGSPYSMDANLLHISYEGRHLEDPSAEAEEDMWRWTVSPEKAPDAAEYIELTYAKGDVVAIDGQQ
MPAHEVLAKLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTILLKAHRAIESITLDREVAHLKDDLMPRYASLIYNG
YWWAPERRALQVLIDHTQAHVNGTVRLKLYKGNVIVVGRDSKNDSLFDSTIATFEDDAGAYDQKDAGGFIKLNALRMRIA
AKLDARRK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=416, Percent_Identity=44.9519230769231, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI16950633, Length=416, Percent_Identity=44.9519230769231, Blast_Score=346, Evalue=3e-95,
Organism=Escherichia coli, GI1789563, Length=426, Percent_Identity=25.1173708920188, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324514, Length=408, Percent_Identity=44.8529411764706, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI21358151, Length=407, Percent_Identity=45.2088452088452, Blast_Score=341, Evalue=5e-94,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_THIDA (Q3SHT1)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315607.2
- HSSP:   Q9X2A1
- ProteinModelPortal:   Q3SHT1
- SMR:   Q3SHT1
- STRING:   Q3SHT1
- GeneID:   3671514
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1849
- NMPDR:   fig|292415.3.peg.1945
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- PhylomeDB:   Q3SHT1
- ProtClustDB:   PRK00509
- BioCyc:   TDEN292415:TBD_1849-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45870; Mature: 45739

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 37-37 BINDING 90-90 BINDING 95-95 BINDING 120-120 BINDING 122-122 BINDING 126-126 BINDING 126-126 BINDING 127-127 BINDING 130-130 BINDING 182-182 BINDING 191-191 BINDING 267-267 BINDING 279-279

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEELEPARQKALQFGIKPE
CCCHHEEHHEECCCCCHHHHHHHHHHCEEEEEEEEEECCCCCCCCCHHHHHHHHCCCCCC
QIYIDDLREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIAKRLIEIVNATGADAICHG
EEEHHHHHHHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHHHHHHCCCCCCCEEEC
ATGKGNDQVRFELGAYALKPDVKVIAPWREWDLLSREKLLAYAESHGIPIDMKHRQGGSP
CCCCCCCEEEEEECCEEECCCCEEEECCHHHCHHHHHHHHHHHHHCCCCEEECCCCCCCC
YSMDANLLHISYEGRHLEDPSAEAEEDMWRWTVSPEKAPDAAEYIELTYAKGDVVAIDGQ
CCCCCEEEEEEECCCCCCCCCCCCCHHHEEEECCCCCCCCHHHEEEEEEECCCEEEECCC
QMPAHEVLAKLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTILLKAHRAIESITLD
CCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHCC
REVAHLKDDLMPRYASLIYNGYWWAPERRALQVLIDHTQAHVNGTVRLKLYKGNVIVVGR
HHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEEC
DSKNDSLFDSTIATFEDDAGAYDQKDAGGFIKLNALRMRIAAKLDARRK
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDIKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEELEPARQKALQFGIKPE
CCHHEEHHEECCCCCHHHHHHHHHHCEEEEEEEEEECCCCCCCCCHHHHHHHHCCCCCC
QIYIDDLREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIAKRLIEIVNATGADAICHG
EEEHHHHHHHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHHHHHHCCCCCCCEEEC
ATGKGNDQVRFELGAYALKPDVKVIAPWREWDLLSREKLLAYAESHGIPIDMKHRQGGSP
CCCCCCCEEEEEECCEEECCCCEEEECCHHHCHHHHHHHHHHHHHCCCCEEECCCCCCCC
YSMDANLLHISYEGRHLEDPSAEAEEDMWRWTVSPEKAPDAAEYIELTYAKGDVVAIDGQ
CCCCCEEEEEEECCCCCCCCCCCCCHHHEEEECCCCCCCCHHHEEEEEEECCCEEEECCC
QMPAHEVLAKLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTILLKAHRAIESITLD
CCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHCC
REVAHLKDDLMPRYASLIYNGYWWAPERRALQVLIDHTQAHVNGTVRLKLYKGNVIVVGR
HHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEEC
DSKNDSLFDSTIATFEDDAGAYDQKDAGGFIKLNALRMRIAAKLDARRK
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA