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The map label for this gene is gbpR [H]

Identifier: 73539685

GI number: 73539685

Start: 2703536

End: 2704477

Strand: Direct

Name: gbpR [H]

Synonym: Reut_B5865

Alternate gene names: 73539685

Gene position: 2703536-2704477 (Clockwise)

Preceding gene: 73539679

Following gene: 73539686

Centisome position: 99.17

GC content: 68.58

Gene sequence:

>942_bases
ATGGACTGGACCCAGCGCCTGCGTCTGCGCAACCTGCAGATGCTCCTCAGCCTCGCCGAAACCGGCAACATGAGCCAGTC
GGCGGCGCTGCTCAACACCACGCAGCCGGGGCTATCGAAATGGCTAAAGGACCTGGAAGACGACATCGGGCTGCCGCTGT
TCGAGCGGCAGGCGCGCGGACTGCAGCCGACCCCCTACGGCGAGTCGCTGATCGAGCACGCGCGCCGCATTACCGCGCAG
CTCGACACGGCGCGCGATGACCTGGCAGCGATGCGGGAGGGCGGTAGCGGGCTGGTGGTGGTCGGCACGTCGGGCGCGTC
GGCTGCCGACACGGTGCCGCTGGCCGTGCTGCAACTGCTGCAGCGCATGCCGCGCGCGCAGGTTCGGCTCGTCGAAACGA
CCATGGACCGGCTCATGAACCAGCTTGCGCACAATGAGCTGGACATTGTGGTCGGGCGGTCCGCGCCCGAGCTCCAGGAC
GCGCAGACCATTGGCGAATCGCTGTACCTCGAACCGATCCATTTCGTGGCACGGCCACGCCACCCGCTGGCCACGCCGAA
CACGGTGACCTGGGAGGCGCTGCAAAGCTACCGCTGGGTCGTCTGGCCGCGCGGCACGCCGATCCGCAATGCGCTCGAGG
CGGCGCTGGCCAGCGCCGGGCGGCCGTTGCCGGCGGACTGCGTGGAATCGAACTCCACGGTTCTCAACCTCACGCTACTC
AACAGCAGCGACATGATCGGCCTGGCCTCGCACCGGACCGCGCGCCGGCTGGAAGAACTTGGCGCGCTGCGCATTGTGCC
GCTGCGCCTCGCCGGGTTTGGCTCGGTGTCGATGTACTGGCGCGAAGATTCGGTCAATCGTTCCGCCGTCGCCGCAATGC
TCGACTGCCTCCGGCGCGCCGCCGACCGAGAAGTGCCGGACGCTGTTGTCCCCACGCCTTGA

Upstream 100 bases:

>100_bases
GGCTGTTGCTTGGAGTTATACGTCCCTCGATATAAGATTTGAAATGAATAAATCCTCTTATCTATACGATTCATCGTATA
GATGAACCGAGCGATCACAC

Downstream 100 bases:

>100_bases
GCGGTTTGGCTTTGGCGCCTACATTGGATGCTCCACGCGCCGGCGCTCCGGCGCGCCGCACATCCACCATGGAGGGGCGA
CATGGAATTCCTGAATTCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Galactose-binding protein regulator; GBP regulator [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MDWTQRLRLRNLQMLLSLAETGNMSQSAALLNTTQPGLSKWLKDLEDDIGLPLFERQARGLQPTPYGESLIEHARRITAQ
LDTARDDLAAMREGGSGLVVVGTSGASAADTVPLAVLQLLQRMPRAQVRLVETTMDRLMNQLAHNELDIVVGRSAPELQD
AQTIGESLYLEPIHFVARPRHPLATPNTVTWEALQSYRWVVWPRGTPIRNALEAALASAGRPLPADCVESNSTVLNLTLL
NSSDMIGLASHRTARRLEELGALRIVPLRLAGFGSVSMYWREDSVNRSAVAAMLDCLRRAADREVPDAVVPTP

Sequences:

>Translated_313_residues
MDWTQRLRLRNLQMLLSLAETGNMSQSAALLNTTQPGLSKWLKDLEDDIGLPLFERQARGLQPTPYGESLIEHARRITAQ
LDTARDDLAAMREGGSGLVVVGTSGASAADTVPLAVLQLLQRMPRAQVRLVETTMDRLMNQLAHNELDIVVGRSAPELQD
AQTIGESLYLEPIHFVARPRHPLATPNTVTWEALQSYRWVVWPRGTPIRNALEAALASAGRPLPADCVESNSTVLNLTLL
NSSDMIGLASHRTARRLEELGALRIVPLRLAGFGSVSMYWREDSVNRSAVAAMLDCLRRAADREVPDAVVPTP
>Mature_313_residues
MDWTQRLRLRNLQMLLSLAETGNMSQSAALLNTTQPGLSKWLKDLEDDIGLPLFERQARGLQPTPYGESLIEHARRITAQ
LDTARDDLAAMREGGSGLVVVGTSGASAADTVPLAVLQLLQRMPRAQVRLVETTMDRLMNQLAHNELDIVVGRSAPELQD
AQTIGESLYLEPIHFVARPRHPLATPNTVTWEALQSYRWVVWPRGTPIRNALEAALASAGRPLPADCVESNSTVLNLTLL
NSSDMIGLASHRTARRLEELGALRIVPLRLAGFGSVSMYWREDSVNRSAVAAMLDCLRRAADREVPDAVVPTP

Specific function: Does not seem to be required for sbpA expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081904, Length=302, Percent_Identity=30.1324503311258, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI145693105, Length=192, Percent_Identity=27.6041666666667, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787601, Length=234, Percent_Identity=23.9316239316239, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787530, Length=221, Percent_Identity=25.7918552036199, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI157672245, Length=264, Percent_Identity=26.1363636363636, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788297, Length=146, Percent_Identity=28.7671232876712, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367136, Length=207, Percent_Identity=29.4685990338164, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789506, Length=244, Percent_Identity=22.9508196721311, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790399, Length=214, Percent_Identity=25.2336448598131, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789440, Length=159, Percent_Identity=26.4150943396226, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788887, Length=197, Percent_Identity=25.8883248730964, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788296, Length=226, Percent_Identity=25.2212389380531, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788748, Length=212, Percent_Identity=24.5283018867925, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787806, Length=124, Percent_Identity=29.0322580645161, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34392; Mature: 34392

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWTQRLRLRNLQMLLSLAETGNMSQSAALLNTTQPGLSKWLKDLEDDIGLPLFERQARG
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCC
LQPTPYGESLIEHARRITAQLDTARDDLAAMREGGSGLVVVGTSGASAADTVPLAVLQLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
QRMPRAQVRLVETTMDRLMNQLAHNELDIVVGRSAPELQDAQTIGESLYLEPIHFVARPR
HHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
HPLATPNTVTWEALQSYRWVVWPRGTPIRNALEAALASAGRPLPADCVESNSTVLNLTLL
CCCCCCCCEEHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHCCCCEEEEEEEE
NSSDMIGLASHRTARRLEELGALRIVPLRLAGFGSVSMYWREDSVNRSAVAAMLDCLRRA
CCCCCEEHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
ADREVPDAVVPTP
HCCCCCCCCCCCC
>Mature Secondary Structure
MDWTQRLRLRNLQMLLSLAETGNMSQSAALLNTTQPGLSKWLKDLEDDIGLPLFERQARG
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCC
LQPTPYGESLIEHARRITAQLDTARDDLAAMREGGSGLVVVGTSGASAADTVPLAVLQLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHH
QRMPRAQVRLVETTMDRLMNQLAHNELDIVVGRSAPELQDAQTIGESLYLEPIHFVARPR
HHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
HPLATPNTVTWEALQSYRWVVWPRGTPIRNALEAALASAGRPLPADCVESNSTVLNLTLL
CCCCCCCCEEHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHCCCCEEEEEEEE
NSSDMIGLASHRTARRLEELGALRIVPLRLAGFGSVSMYWREDSVNRSAVAAMLDCLRRA
CCCCCEEHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
ADREVPDAVVPTP
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10361275 [H]