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The map label for this gene is xylR [H]

Identifier: 73539493

GI number: 73539493

Start: 2502258

End: 2503952

Strand: Reverse

Name: xylR [H]

Synonym: Reut_B5671

Alternate gene names: 73539493

Gene position: 2503952-2502258 (Counterclockwise)

Preceding gene: 73539495

Following gene: 73539492

Centisome position: 91.85

GC content: 66.49

Gene sequence:

>1695_bases
GTGGAAGCCAAGCTCAGTTACCCTCCGAATGCCGATCTGCGGCACCTCCTGCGCTTCGATCCCGAGAGCGGCGCCATCTG
GCTGGGCGAACGCCGCATGCTGCTCCTGCATACTGCCGCACTGAGCGGCCTGAGGCAGGACCTGATCCATTCGGTGGGCC
CCGTGCACGCGCGCAGACTGCTCACGCGCATGGGATATGCCTCTGGCCGGCGCGACGCTGAATTTGCACGCACGACGCGC
GGTGCACTCTCGCTCGACGCCGCATTCCTGGTCGGGCCGCAACTGCACATGCTCGAAGGCGCTGCGTGTGTGACGCCGAT
CCAACTCGATTTCGACGCCGCCACAGGCCGCTTCTCCGGAGAATTCCGTTGGGACAACTCGTGGGAAGCCTATGCCCACC
GGCACGCCTTCGGGCTCGCGGAAACGCCTGCCTGCTGGGTTCTGCTCGGATACGCCAGCGGCTACACGAGTGCCTTCATG
GGCCGCCTCATCGTCTTCAAGGAAACATGCTGCGCGGCCTGCGGCGCCGACCACTGCCAGATCGTTGGCCGGCCTGTCGA
CGAATGGCCCGACGCCGCCGAACAGCGCCAGTACTTCGAGGACGATTCCCTTCTCGACACCATCGCCGCGCTCAACGAGC
AGGTGCAGGCGCTGGTGACGACCATCGAGTCAGCAGGCGAGCCGGGGATCTCCGCCGGCATGCTTGTCGGCGCCTCGCCC
GCATTCCAGGAAGCCCACGCGTTGTTGAACCGCGCCGCCGCCACGCAGGTGACTGTATTGCTGACCGGCGAGACGGGCGT
GGGCAAGGAACGCTTCGCCCGCGCCTTGCACCAAGGATCGGCCCGCGCCGGCGGTCCCTTCATCGCCGTCAACTGCGCCG
CGCTGCCCGCTGACCTGATCGAGGCCGAATTGTTTGGCGTCGAGCGGGGCGCCTACACCGGCGCGCACGTCAGCCGCAGC
GGCCGCTTCGAGCGGGCAGACGGCGGCACGCTCTTCCTCGATGAAGTCGGCGACCTGCCACTGCCGTTACAGGCCAAGCT
GCTACGCGTGCTGCAGGACGGCGAGATCGAGCGCCTCGGCGCGGAGACCGCGCGCAAGGTCAATGTGCGCCTCGTCACGG
CAACGAATGTCGACCTTGAGGCAGCAGTGTCGCAGGGACGGTTCCGGCGCGACCTGTTGTACCGACTAAACGTCTATCCG
ATCTGCATTCCGCCACTGCGCGAGCGAACGGCCGATATCGAGCCACTGGCGAACCACCTGCTGGCGCGCTTCAGCGCGCT
CCACCAGAAGCGCCTTTCCGGGATTAGCGAACGCGCTATGCAGGCGCTCGTGCACCACGATTGGCCCGGCAACGTGCGTG
AACTCGAGAACCTGATCGAGCGCGGTGTCATCCTTTCGTCGCAAGGTGGAACCATCGAACTGGAACATCTCTTTCCGCAC
CGGCCACCGGGGATACAGGCAACGGTAAGCGCGAGAGGCCGGCTGGCGCCGGTGCGGCCGGACAGCGAGCAGGCCCTCTG
CGTGCGCATTCTCGATAGCGGGCTCAGCTTGGAATCGCTGGAGAGCCAGCTTCTCGAGCTTGCCGTCGAGCGCGCCGGCG
GCAACCTGTCAGGCGCGGCGCGGCACCTCGGCCTGACCCGCCCGCAACTGGCCTATCGCCTCAAGCGCCAAGAAGACAAA
GACAGCGAAGCATGA

Upstream 100 bases:

>100_bases
TCGAATCCGGTGTGCGCGTGGGACGGTCCTTGCATCGCCCGGTAGGATCCACCAGAATATCGAGATTCCACCCACCACCT
AACAACGGGGGACGCACGCC

Downstream 100 bases:

>100_bases
CAGCCGCCAAACGCCGCAGCGCCCGCGACAGCGTGCCAGACGACCCTCGGCCTCCCGAGCCCTTCGCGCCGCGCACCCTG
TGCGAGCGCGCCTATCTTCA

Product: Fis-type helix-turn-helix domain-containing protein

Products: NA

Alternate protein names: 67 kDa protein [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MEAKLSYPPNADLRHLLRFDPESGAIWLGERRMLLLHTAALSGLRQDLIHSVGPVHARRLLTRMGYASGRRDAEFARTTR
GALSLDAAFLVGPQLHMLEGAACVTPIQLDFDAATGRFSGEFRWDNSWEAYAHRHAFGLAETPACWVLLGYASGYTSAFM
GRLIVFKETCCAACGADHCQIVGRPVDEWPDAAEQRQYFEDDSLLDTIAALNEQVQALVTTIESAGEPGISAGMLVGASP
AFQEAHALLNRAAATQVTVLLTGETGVGKERFARALHQGSARAGGPFIAVNCAALPADLIEAELFGVERGAYTGAHVSRS
GRFERADGGTLFLDEVGDLPLPLQAKLLRVLQDGEIERLGAETARKVNVRLVTATNVDLEAAVSQGRFRRDLLYRLNVYP
ICIPPLRERTADIEPLANHLLARFSALHQKRLSGISERAMQALVHHDWPGNVRELENLIERGVILSSQGGTIELEHLFPH
RPPGIQATVSARGRLAPVRPDSEQALCVRILDSGLSLESLESQLLELAVERAGGNLSGAARHLGLTRPQLAYRLKRQEDK
DSEA

Sequences:

>Translated_564_residues
MEAKLSYPPNADLRHLLRFDPESGAIWLGERRMLLLHTAALSGLRQDLIHSVGPVHARRLLTRMGYASGRRDAEFARTTR
GALSLDAAFLVGPQLHMLEGAACVTPIQLDFDAATGRFSGEFRWDNSWEAYAHRHAFGLAETPACWVLLGYASGYTSAFM
GRLIVFKETCCAACGADHCQIVGRPVDEWPDAAEQRQYFEDDSLLDTIAALNEQVQALVTTIESAGEPGISAGMLVGASP
AFQEAHALLNRAAATQVTVLLTGETGVGKERFARALHQGSARAGGPFIAVNCAALPADLIEAELFGVERGAYTGAHVSRS
GRFERADGGTLFLDEVGDLPLPLQAKLLRVLQDGEIERLGAETARKVNVRLVTATNVDLEAAVSQGRFRRDLLYRLNVYP
ICIPPLRERTADIEPLANHLLARFSALHQKRLSGISERAMQALVHHDWPGNVRELENLIERGVILSSQGGTIELEHLFPH
RPPGIQATVSARGRLAPVRPDSEQALCVRILDSGLSLESLESQLLELAVERAGGNLSGAARHLGLTRPQLAYRLKRQEDK
DSEA
>Mature_564_residues
MEAKLSYPPNADLRHLLRFDPESGAIWLGERRMLLLHTAALSGLRQDLIHSVGPVHARRLLTRMGYASGRRDAEFARTTR
GALSLDAAFLVGPQLHMLEGAACVTPIQLDFDAATGRFSGEFRWDNSWEAYAHRHAFGLAETPACWVLLGYASGYTSAFM
GRLIVFKETCCAACGADHCQIVGRPVDEWPDAAEQRQYFEDDSLLDTIAALNEQVQALVTTIESAGEPGISAGMLVGASP
AFQEAHALLNRAAATQVTVLLTGETGVGKERFARALHQGSARAGGPFIAVNCAALPADLIEAELFGVERGAYTGAHVSRS
GRFERADGGTLFLDEVGDLPLPLQAKLLRVLQDGEIERLGAETARKVNVRLVTATNVDLEAAVSQGRFRRDLLYRLNVYP
ICIPPLRERTADIEPLANHLLARFSALHQKRLSGISERAMQALVHHDWPGNVRELENLIERGVILSSQGGTIELEHLFPH
RPPGIQATVSARGRLAPVRPDSEQALCVRILDSGLSLESLESQLLELAVERAGGNLSGAARHLGLTRPQLAYRLKRQEDK
DSEA

Specific function: Regulatory protein of the TOL plasmid xyl operons. In the presence of m-xylene or m-methylbenzyl alcohol xylR activates both the xylCMABN operon and the regulatory gene xylS; xylS itself activates the xylXYZLTEGFJQKIH operon. XylR interacts with sigma- 54

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=323, Percent_Identity=45.8204334365325, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1790437, Length=354, Percent_Identity=42.6553672316384, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082117, Length=335, Percent_Identity=43.2835820895522, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1790299, Length=334, Percent_Identity=42.814371257485, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1789087, Length=360, Percent_Identity=41.6666666666667, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1788905, Length=215, Percent_Identity=52.5581395348837, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1789233, Length=339, Percent_Identity=40.7079646017699, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87081872, Length=336, Percent_Identity=42.2619047619048, Blast_Score=219, Evalue=5e-58,
Organism=Escherichia coli, GI87082152, Length=238, Percent_Identity=47.0588235294118, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1786524, Length=336, Percent_Identity=39.8809523809524, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI1787583, Length=229, Percent_Identity=40.6113537117904, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI87081858, Length=310, Percent_Identity=35.1612903225806, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=35.3982300884956, Blast_Score=132, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR004096
- InterPro:   IPR010523
- ProDom:   PD485437 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat; PF02830 V4R; PF06505 XylR_N [H]

EC number: NA

Molecular weight: Translated: 61432; Mature: 61432

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKLSYPPNADLRHLLRFDPESGAIWLGERRMLLLHTAALSGLRQDLIHSVGPVHARRL
CCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH
LTRMGYASGRRDAEFARTTRGALSLDAAFLVGPQLHMLEGAACVTPIQLDFDAATGRFSG
HHHHCCCCCCCCHHHHHHHCCCCEECHHHHCCCCHHHHCCCCEEEEEEEEECCCCCCCCC
EFRWDNSWEAYAHRHAFGLAETPACWVLLGYASGYTSAFMGRLIVFKETCCAACGADHCQ
EEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
IVGRPVDEWPDAAEQRQYFEDDSLLDTIAALNEQVQALVTTIESAGEPGISAGMLVGASP
EECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCH
AFQEAHALLNRAAATQVTVLLTGETGVGKERFARALHQGSARAGGPFIAVNCAALPADLI
HHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHCCHHHH
EAELFGVERGAYTGAHVSRSGRFERADGGTLFLDEVGDLPLPLQAKLLRVLQDGEIERLG
HHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHC
AETARKVNVRLVTATNVDLEAAVSQGRFRRDLLYRLNVYPICIPPLRERTADIEPLANHL
HHHHHEEEEEEEEEECCCHHHHHHCCHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHH
LARFSALHQKRLSGISERAMQALVHHDWPGNVRELENLIERGVILSSQGGTIELEHLFPH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCEEEEEECCCC
RPPGIQATVSARGRLAPVRPDSEQALCVRILDSGLSLESLESQLLELAVERAGGNLSGAA
CCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHH
RHLGLTRPQLAYRLKRQEDKDSEA
HHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEAKLSYPPNADLRHLLRFDPESGAIWLGERRMLLLHTAALSGLRQDLIHSVGPVHARRL
CCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH
LTRMGYASGRRDAEFARTTRGALSLDAAFLVGPQLHMLEGAACVTPIQLDFDAATGRFSG
HHHHCCCCCCCCHHHHHHHCCCCEECHHHHCCCCHHHHCCCCEEEEEEEEECCCCCCCCC
EFRWDNSWEAYAHRHAFGLAETPACWVLLGYASGYTSAFMGRLIVFKETCCAACGADHCQ
EEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
IVGRPVDEWPDAAEQRQYFEDDSLLDTIAALNEQVQALVTTIESAGEPGISAGMLVGASP
EECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCH
AFQEAHALLNRAAATQVTVLLTGETGVGKERFARALHQGSARAGGPFIAVNCAALPADLI
HHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHCCHHHH
EAELFGVERGAYTGAHVSRSGRFERADGGTLFLDEVGDLPLPLQAKLLRVLQDGEIERLG
HHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHC
AETARKVNVRLVTATNVDLEAAVSQGRFRRDLLYRLNVYPICIPPLRERTADIEPLANHL
HHHHHEEEEEEEEEECCCHHHHHHCCHHHHHHHHHCCCEEEECCCHHHHCCCHHHHHHHH
LARFSALHQKRLSGISERAMQALVHHDWPGNVRELENLIERGVILSSQGGTIELEHLFPH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCEEEEEECCCC
RPPGIQATVSARGRLAPVRPDSEQALCVRILDSGLSLESLESQLLELAVERAGGNLSGAA
CCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHH
RHLGLTRPQLAYRLKRQEDKDSEA
HHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3169574; 2993247; 2430049 [H]