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The map label for this gene is ispH [H]

Identifier: 73538723

GI number: 73538723

Start: 1622016

End: 1622987

Strand: Direct

Name: ispH [H]

Synonym: Reut_B4898

Alternate gene names: 73538723

Gene position: 1622016-1622987 (Clockwise)

Preceding gene: 73538721

Following gene: 73538724

Centisome position: 59.5

GC content: 66.26

Gene sequence:

>972_bases
ATGCAGGTGATTCTTGCTCAACCCCGAGGTTTCTGTGCCGGCGTCGTGCGCGCGATCGAAATTGTCGACCGCGCACTCGT
CAAGCATGGAGCTCCGGTGTTCGTGCGGCACGAGATCGTGCATAACAAGCATGTCGTGGAGGGGTTGAAGAACAAGGGCG
CGCGCTTTGTTGAAGAACTCGACGAGGTCCCTGCGGGCGCCGTCACCATCTTCAGCGCGCACGGCGTATCGCGCGCCGTG
GTGGCCGACGCCGGCCAGCGGCAGCTGCACGCCATCGACGCCACCTGCCCGCTGGTGATCAAGGTGCATAACCAGGGCCG
CCAGTACGCGGCCAGCGGCCGGACCGTGATCCTGATCGGCCATGCCGGCCATCCCGAGGTCGAAGGCACCATGGGCCAGA
TTCCCGGCAAGGTGATCCTGGTCCAGAACGAGGCCGAGGTCGAACGGCTCGACCTGCCTGCGGACACCCCGGTCGCCTAC
GTCACCCAGACCACGCTCAGCGTCGACGACACCCGCAATATCATCGCCGCGCTGGCCCGCCGTTTCAGCAACATGGTCGG
CCCCGACACGCGCGACATCTGCTATGCCACGCAGAACCGGCAAAGCGCGGTGCGCGACCTGTGCAAGGTGGCCGACGTGA
TCCTGGTGATCGGCGCCACCAACAGTTCCAATTCCAACCGGCTGCGCGAGATCGGCACTGAAAGCGGCGTGCCCAGCTAC
CTGATCGCCGACGGCAGCGAACTCGATCCGGCGTGGGTGACGGGGGCCAATATCGTCGGCATTACCGCCGGCGCCTCGGC
GCCCGAGGAAATGGTCGAAGACGTGATCAACGCGCTGCGCCACCTGGGCCCGGTGGATGTGTCCACCATGGAAGGCCGCG
AGGAGCATGCCGAATTCCGCCTGCCCGCCGAACTGGCCGATGTCCGGCCTGCCGCAGCCGCGACCGAGCCAGTCGAGAAA
GTCCCCGGCTGA

Upstream 100 bases:

>100_bases
CGGCGCGGCGGGTATATAGTAACCACCATGTGTCGGAAATGTGATTTCTGTACACCATCCCATTCACTGAAACCCATTGA
CGGGTCGGGGGGCCTACCGT

Downstream 100 bases:

>100_bases
ACCAGAATAAAAGGAAGTCCCCTTGCCTATCCCGTTCCTGCAGGTCGCCCGCGTGGGCGCCTACATTGTCGGCAAGCATC
TGGCGCGCCAGAAGCGCTAT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MQVILAQPRGFCAGVVRAIEIVDRALVKHGAPVFVRHEIVHNKHVVEGLKNKGARFVEELDEVPAGAVTIFSAHGVSRAV
VADAGQRQLHAIDATCPLVIKVHNQGRQYAASGRTVILIGHAGHPEVEGTMGQIPGKVILVQNEAEVERLDLPADTPVAY
VTQTTLSVDDTRNIIAALARRFSNMVGPDTRDICYATQNRQSAVRDLCKVADVILVIGATNSSNSNRLREIGTESGVPSY
LIADGSELDPAWVTGANIVGITAGASAPEEMVEDVINALRHLGPVDVSTMEGREEHAEFRLPAELADVRPAAAATEPVEK
VPG

Sequences:

>Translated_323_residues
MQVILAQPRGFCAGVVRAIEIVDRALVKHGAPVFVRHEIVHNKHVVEGLKNKGARFVEELDEVPAGAVTIFSAHGVSRAV
VADAGQRQLHAIDATCPLVIKVHNQGRQYAASGRTVILIGHAGHPEVEGTMGQIPGKVILVQNEAEVERLDLPADTPVAY
VTQTTLSVDDTRNIIAALARRFSNMVGPDTRDICYATQNRQSAVRDLCKVADVILVIGATNSSNSNRLREIGTESGVPSY
LIADGSELDPAWVTGANIVGITAGASAPEEMVEDVINALRHLGPVDVSTMEGREEHAEFRLPAELADVRPAAAATEPVEK
VPG
>Mature_323_residues
MQVILAQPRGFCAGVVRAIEIVDRALVKHGAPVFVRHEIVHNKHVVEGLKNKGARFVEELDEVPAGAVTIFSAHGVSRAV
VADAGQRQLHAIDATCPLVIKVHNQGRQYAASGRTVILIGHAGHPEVEGTMGQIPGKVILVQNEAEVERLDLPADTPVAY
VTQTTLSVDDTRNIIAALARRFSNMVGPDTRDICYATQNRQSAVRDLCKVADVILVIGATNSSNSNRLREIGTESGVPSY
LIADGSELDPAWVTGANIVGITAGASAPEEMVEDVINALRHLGPVDVSTMEGREEHAEFRLPAELADVRPAAAATEPVEK
VPG

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=313, Percent_Identity=52.0766773162939, Blast_Score=324, Evalue=5e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34417; Mature: 34417

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVILAQPRGFCAGVVRAIEIVDRALVKHGAPVFVRHEIVHNKHVVEGLKNKGARFVEEL
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHH
DEVPAGAVTIFSAHGVSRAVVADAGQRQLHAIDATCPLVIKVHNQGRQYAASGRTVILIG
HHCCCCEEEEEECCCCCCHHHHCCCCCEEEEECCCCEEEEEECCCCCEEECCCCEEEEEE
HAGHPEVEGTMGQIPGKVILVQNEAEVERLDLPADTPVAYVTQTTLSVDDTRNIIAALAR
CCCCCCCCCHHHCCCCEEEEEECCCCHHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHHH
RFSNMVGPDTRDICYATQNRQSAVRDLCKVADVILVIGATNSSNSNRLREIGTESGVPSY
HHHHHCCCCCCHHEEECCCHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHCCCCCCCCE
LIADGSELDPAWVTGANIVGITAGASAPEEMVEDVINALRHLGPVDVSTMEGREEHAEFR
EEECCCCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHCCCCHHHCCEE
LPAELADVRPAAAATEPVEKVPG
CCHHHHHCCCCHHCCCCHHHCCC
>Mature Secondary Structure
MQVILAQPRGFCAGVVRAIEIVDRALVKHGAPVFVRHEIVHNKHVVEGLKNKGARFVEEL
CEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHH
DEVPAGAVTIFSAHGVSRAVVADAGQRQLHAIDATCPLVIKVHNQGRQYAASGRTVILIG
HHCCCCEEEEEECCCCCCHHHHCCCCCEEEEECCCCEEEEEECCCCCEEECCCCEEEEEE
HAGHPEVEGTMGQIPGKVILVQNEAEVERLDLPADTPVAYVTQTTLSVDDTRNIIAALAR
CCCCCCCCCHHHCCCCEEEEEECCCCHHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHHH
RFSNMVGPDTRDICYATQNRQSAVRDLCKVADVILVIGATNSSNSNRLREIGTESGVPSY
HHHHHCCCCCCHHEEECCCHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHCCCCCCCCE
LIADGSELDPAWVTGANIVGITAGASAPEEMVEDVINALRHLGPVDVSTMEGREEHAEFR
EEECCCCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHCCCCHHHCCEE
LPAELADVRPAAAATEPVEKVPG
CCHHHHHCCCCHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA