Click here to switch to the map view.

The map label for this gene is fadJ [H]

Identifier: 73538624

GI number: 73538624

Start: 1498686

End: 1500026

Strand: Direct

Name: fadJ [H]

Synonym: Reut_B4799

Alternate gene names: 73538624

Gene position: 1498686-1500026 (Clockwise)

Preceding gene: 73538623

Following gene: 73538625

Centisome position: 54.97

GC content: 65.92

Gene sequence:

>1341_bases
ATGACTACAACTCCAACCGACGCTGAAGCACAGGCTACGGCAGACTACGTCCGCTTCGCGCAGGCGGAAGCAGCCCATAT
ACCCGACTTCCCAAAGGACTTGCCGCTGCGTCCCGTGAACCATGTTGCGGTAATCGGCGCCGGCACCATGGGCGGTGGCA
TTGCGATGGCACTGGCCAATATCGGCATTCCGGTCACGCTGATCGACAGCAGCGAGAAAGGCCTGGAGACCGGCTTGCGC
CGCGTGCGGGACAACTACGCCGGCAGCGTCTCCCGCGGCAAGCTCGAACCGGCAGCCATGGAAACGAGGCTGTCGCTGAT
CAAAGGCTCCGGTGCCCTCGCAGATGCCGCTTCCGCCGACATGGTGATTGAAGCGGTATTCGAGGACATGGCGCTCAAGC
AGCAGGTGTTCCGCGATCTCGATGCGCGGTGCAAACCCGGGGCGATCCTGGCCACCAACACTTCGGGACTCGATGTGGAT
GCCATCGCGGCCGTCACGAAGCGCCCGCAGGACGTGGTCGGGGCGCACTTCTTCAGTCCGGCCCATGTGATGCGCCTGCT
CGAAGTCGTGCGTGCGCGGGACACCGCGCCGGACGTGATCGCCACGCTGATGGACCTCGGCCGGCGCATGGGCAAGACCG
CCGTGCTCGCTCGCGTCTACCCCGGCTTCATCGGCAATGCGCTGTTCCGCAACTACACGCGGGAGGCGCATTTCCTGGTC
GAGGAAGGCGCGCTGCCGCACGAGGTGGACCAGGCGCTGACCGACTTCGGCTACGCGATGGGCATCTTCGCCGTGCACGA
CATGGCCGGCAACGACGTGGGGTACCAGACACGCAAGGCGCAGATCGCCACCCGGCCGCAGGACCGGCGCTGGAACGACC
TGATCCTGAAACTGACCGAACTTGGGCGGCTGGGACAGAAAAGCGGCAAGGGCTGGTACCGCTATGAACCGGGCAGCCGC
CAGCCGCTGCGCGATCCGGAGGTGGAAACGTTCATCGAACAGGAATCGGCGCGGCTCGGCATGGTGCGGCGGCCCATCGA
CGCCGAGGAAATCATCAAGCGCTGCGTCTACGGCATGGTCAACGAGGGCGCGAAGCTGCTGGAGGCCGGCGTAGCGCTGC
GGCCCAGCGATATCGACACGGTCTACCTGACCGGCTACGGCTTTCCCGCGCGCCATGGCGGGCCGATGTACTACGCCGAT
CGCATCGGCCTGCGCGAGGTGTACGCCGACATCGAACGCTTCCACGCCGAGCACGGCTACTGGTGGGAACCGGCCCCGCT
GCTGGTCCGGCTGGCGCGCGATGGCAAGACCTTTGCGGACTACCAGCGCGGCAACGCTTAA

Upstream 100 bases:

>100_bases
GCGCCCGCCCGAAGCGGCGGAACGCCTGCGCGATTTCGTCGAAGGCCGCGCCAAACCGCTCGCCGTTCCGGGCGAGAAGT
CCGAATAAGCGAGCAATGCC

Downstream 100 bases:

>100_bases
GGCTCGCGGCACCCGTTTGCATTTTTGATACGCACTAACGTATACCACTATTTTTTATATACATACCAGTAAGCGATAGT
TCTGCCACTTCCCGGTGCGG

Product: 3-hydroxyacyl-CoA dehydrogenase

Products: NA

Alternate protein names: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTTTPTDAEAQATADYVRFAQAEAAHIPDFPKDLPLRPVNHVAVIGAGTMGGGIAMALANIGIPVTLIDSSEKGLETGLR
RVRDNYAGSVSRGKLEPAAMETRLSLIKGSGALADAASADMVIEAVFEDMALKQQVFRDLDARCKPGAILATNTSGLDVD
AIAAVTKRPQDVVGAHFFSPAHVMRLLEVVRARDTAPDVIATLMDLGRRMGKTAVLARVYPGFIGNALFRNYTREAHFLV
EEGALPHEVDQALTDFGYAMGIFAVHDMAGNDVGYQTRKAQIATRPQDRRWNDLILKLTELGRLGQKSGKGWYRYEPGSR
QPLRDPEVETFIEQESARLGMVRRPIDAEEIIKRCVYGMVNEGAKLLEAGVALRPSDIDTVYLTGYGFPARHGGPMYYAD
RIGLREVYADIERFHAEHGYWWEPAPLLVRLARDGKTFADYQRGNA

Sequences:

>Translated_446_residues
MTTTPTDAEAQATADYVRFAQAEAAHIPDFPKDLPLRPVNHVAVIGAGTMGGGIAMALANIGIPVTLIDSSEKGLETGLR
RVRDNYAGSVSRGKLEPAAMETRLSLIKGSGALADAASADMVIEAVFEDMALKQQVFRDLDARCKPGAILATNTSGLDVD
AIAAVTKRPQDVVGAHFFSPAHVMRLLEVVRARDTAPDVIATLMDLGRRMGKTAVLARVYPGFIGNALFRNYTREAHFLV
EEGALPHEVDQALTDFGYAMGIFAVHDMAGNDVGYQTRKAQIATRPQDRRWNDLILKLTELGRLGQKSGKGWYRYEPGSR
QPLRDPEVETFIEQESARLGMVRRPIDAEEIIKRCVYGMVNEGAKLLEAGVALRPSDIDTVYLTGYGFPARHGGPMYYAD
RIGLREVYADIERFHAEHGYWWEPAPLLVRLARDGKTFADYQRGNA
>Mature_445_residues
TTTPTDAEAQATADYVRFAQAEAAHIPDFPKDLPLRPVNHVAVIGAGTMGGGIAMALANIGIPVTLIDSSEKGLETGLRR
VRDNYAGSVSRGKLEPAAMETRLSLIKGSGALADAASADMVIEAVFEDMALKQQVFRDLDARCKPGAILATNTSGLDVDA
IAAVTKRPQDVVGAHFFSPAHVMRLLEVVRARDTAPDVIATLMDLGRRMGKTAVLARVYPGFIGNALFRNYTREAHFLVE
EGALPHEVDQALTDFGYAMGIFAVHDMAGNDVGYQTRKAQIATRPQDRRWNDLILKLTELGRLGQKSGKGWYRYEPGSRQ
PLRDPEVETFIEQESARLGMVRRPIDAEEIIKRCVYGMVNEGAKLLEAGVALRPSDIDTVYLTGYGFPARHGGPMYYADR
IGLREVYADIERFHAEHGYWWEPAPLLVRLARDGKTFADYQRGNA

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI261878539, Length=416, Percent_Identity=41.5865384615385, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI68989263, Length=416, Percent_Identity=41.5865384615385, Blast_Score=322, Evalue=6e-88,
Organism=Homo sapiens, GI20127408, Length=398, Percent_Identity=30.9045226130653, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI296179429, Length=295, Percent_Identity=34.2372881355932, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI296179427, Length=312, Percent_Identity=31.7307692307692, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI115430219, Length=222, Percent_Identity=29.7297297297297, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=454, Percent_Identity=32.3788546255507, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1790281, Length=387, Percent_Identity=30.749354005168, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1787661, Length=281, Percent_Identity=38.7900355871886, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71985923, Length=414, Percent_Identity=32.3671497584541, Blast_Score=228, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI71985930, Length=371, Percent_Identity=32.6145552560647, Blast_Score=215, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17508953, Length=408, Percent_Identity=33.578431372549, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17508951, Length=408, Percent_Identity=33.578431372549, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17558304, Length=437, Percent_Identity=33.1807780320366, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI25144276, Length=287, Percent_Identity=34.8432055749129, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17549919, Length=291, Percent_Identity=34.7079037800687, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17563036, Length=293, Percent_Identity=35.4948805460751, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17553560, Length=296, Percent_Identity=33.1081081081081, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24583077, Length=410, Percent_Identity=31.9512195121951, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24583079, Length=410, Percent_Identity=31.9512195121951, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI19921000, Length=410, Percent_Identity=31.9512195121951, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45549573, Length=222, Percent_Identity=27.9279279279279, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR012802
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =1.1.1.35 [H]

Molecular weight: Translated: 48930; Mature: 48799

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00732 RIBOSOMAL_S16

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPTDAEAQATADYVRFAQAEAAHIPDFPKDLPLRPVNHVAVIGAGTMGGGIAMALAN
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
IGIPVTLIDSSEKGLETGLRRVRDNYAGSVSRGKLEPAAMETRLSLIKGSGALADAASAD
CCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
MVIEAVFEDMALKQQVFRDLDARCKPGAILATNTSGLDVDAIAAVTKRPQDVVGAHFFSP
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHCCH
AHVMRLLEVVRARDTAPDVIATLMDLGRRMGKTAVLARVYPGFIGNALFRNYTREAHFLV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
EEGALPHEVDQALTDFGYAMGIFAVHDMAGNDVGYQTRKAQIATRPQDRRWNDLILKLTE
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHH
LGRLGQKSGKGWYRYEPGSRQPLRDPEVETFIEQESARLGMVRRPIDAEEIIKRCVYGMV
HHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
NEGAKLLEAGVALRPSDIDTVYLTGYGFPARHGGPMYYADRIGLREVYADIERFHAEHGY
HCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHCCCC
WWEPAPLLVRLARDGKTFADYQRGNA
CCCCHHHHHHHHHCCCHHHHHCCCCC
>Mature Secondary Structure 
TTTPTDAEAQATADYVRFAQAEAAHIPDFPKDLPLRPVNHVAVIGAGTMGGGIAMALAN
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
IGIPVTLIDSSEKGLETGLRRVRDNYAGSVSRGKLEPAAMETRLSLIKGSGALADAASAD
CCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
MVIEAVFEDMALKQQVFRDLDARCKPGAILATNTSGLDVDAIAAVTKRPQDVVGAHFFSP
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHCCH
AHVMRLLEVVRARDTAPDVIATLMDLGRRMGKTAVLARVYPGFIGNALFRNYTREAHFLV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
EEGALPHEVDQALTDFGYAMGIFAVHDMAGNDVGYQTRKAQIATRPQDRRWNDLILKLTE
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHH
LGRLGQKSGKGWYRYEPGSRQPLRDPEVETFIEQESARLGMVRRPIDAEEIIKRCVYGMV
HHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
NEGAKLLEAGVALRPSDIDTVYLTGYGFPARHGGPMYYADRIGLREVYADIERFHAEHGY
HCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHCCCC
WWEPAPLLVRLARDGKTFADYQRGNA
CCCCHHHHHHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA