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The map label for this gene is paaF [C]

Identifier: 73538623

GI number: 73538623

Start: 1497876

End: 1498673

Strand: Direct

Name: paaF [C]

Synonym: Reut_B4798

Alternate gene names: 73538623

Gene position: 1497876-1498673 (Clockwise)

Preceding gene: 73538622

Following gene: 73538624

Centisome position: 54.94

GC content: 66.92

Gene sequence:

>798_bases
ATGAGCACCGAACTGGTGACGTATGAACTCGACGGCAATGTCGCGCTGATCGGCCTGAACCGGCCAGAGAAGCGCAATGC
CATCAACGAAGACGTGGTCTGGGCGCTGCACGACGCCGTCGTGCGTGCCGGCGAGGAAGCCGATGTGGGCGTGCTGTTCG
GGCACGGCAGCAACTTCTGCGCAGGACTCGATCTGAAGGAAGCGCTGGCGCGAGCGTCCGGTGAGCAACCACGGCCCCGC
AAGCGCACGCGCCATTCTTGGCACACCGTGTTCGACGGTATCGCGCGCGGGCCCATTCCGTTCGTCGCCGCGTTGCATGG
TGCGGTCGTGGGCGGTGGACTGGAACTGGCGACCGCTGCGCACATCCGCGTCGCCGACGAATCGGCGCTGTTCGGCCTGC
CCGAAGGACAGCGCGGCATCTTTGTCGGCGGTGGCGGCACCGTGCGCATCCAGCGCGTGGTCGGCTACACCGTGATGGCC
GACATGATGCTGACCGGGCGCCTGCTCAATGCACAGGAAGGCGAGCGCGAACACATCGTGCGCTACGTGGTGCCGCAGGG
CCAGGCGCTCGCCAAGGCGCGCGAGCTGGCCGCGCTGATCGCGCGCAATACCGCCGATACGAACTGGCGCATCACCAACG
TACTGCCGCGCATCAACGACCTGTCCCACGACGACGGCCTGTTCGTCGAGTACCTGACCTCGAACATCCCGCGCCCGCCC
GAAGCGGCGGAACGCCTGCGCGATTTCGTCGAAGGCCGCGCCAAACCGCTCGCCGTTCCGGGCGAGAAGTCCGAATAA

Upstream 100 bases:

>100_bases
ACAACCGGATGATGGTGTGCTGCTCCGGCGCGAAGACCCGCAAGCTGGTGCTGGATCTGTAGCCGCACCGTCGAACCACG
TAATGAACAAGGAGCATGAG

Downstream 100 bases:

>100_bases
GCGAGCAATGCCATGACTACAACTCCAACCGACGCTGAAGCACAGGCTACGGCAGACTACGTCCGCTTCGCGCAGGCGGA
AGCAGCCCATATACCCGACT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSTELVTYELDGNVALIGLNRPEKRNAINEDVVWALHDAVVRAGEEADVGVLFGHGSNFCAGLDLKEALARASGEQPRPR
KRTRHSWHTVFDGIARGPIPFVAALHGAVVGGGLELATAAHIRVADESALFGLPEGQRGIFVGGGGTVRIQRVVGYTVMA
DMMLTGRLLNAQEGEREHIVRYVVPQGQALAKARELAALIARNTADTNWRITNVLPRINDLSHDDGLFVEYLTSNIPRPP
EAAERLRDFVEGRAKPLAVPGEKSE

Sequences:

>Translated_265_residues
MSTELVTYELDGNVALIGLNRPEKRNAINEDVVWALHDAVVRAGEEADVGVLFGHGSNFCAGLDLKEALARASGEQPRPR
KRTRHSWHTVFDGIARGPIPFVAALHGAVVGGGLELATAAHIRVADESALFGLPEGQRGIFVGGGGTVRIQRVVGYTVMA
DMMLTGRLLNAQEGEREHIVRYVVPQGQALAKARELAALIARNTADTNWRITNVLPRINDLSHDDGLFVEYLTSNIPRPP
EAAERLRDFVEGRAKPLAVPGEKSE
>Mature_264_residues
STELVTYELDGNVALIGLNRPEKRNAINEDVVWALHDAVVRAGEEADVGVLFGHGSNFCAGLDLKEALARASGEQPRPRK
RTRHSWHTVFDGIARGPIPFVAALHGAVVGGGLELATAAHIRVADESALFGLPEGQRGIFVGGGGTVRIQRVVGYTVMAD
MMLTGRLLNAQEGEREHIVRYVVPQGQALAKARELAALIARNTADTNWRITNVLPRINDLSHDDGLFVEYLTSNIPRPPE
AAERLRDFVEGRAKPLAVPGEKSE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=196, Percent_Identity=34.1836734693878, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI70995211, Length=187, Percent_Identity=31.0160427807487, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI4502327, Length=223, Percent_Identity=30.9417040358744, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI20127408, Length=265, Percent_Identity=25.6603773584906, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI31542718, Length=198, Percent_Identity=28.2828282828283, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI37594469, Length=204, Percent_Identity=26.9607843137255, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI37594471, Length=204, Percent_Identity=26.9607843137255, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI68989263, Length=227, Percent_Identity=31.7180616740088, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221136756, Length=201, Percent_Identity=23.3830845771144, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=201, Percent_Identity=23.3830845771144, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221307494, Length=201, Percent_Identity=23.3830845771144, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=196, Percent_Identity=34.1836734693878, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=35.1485148514851, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI221142681, Length=190, Percent_Identity=34.2105263157895, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790281, Length=203, Percent_Identity=25.615763546798, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=204, Percent_Identity=37.2549019607843, Blast_Score=113, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=194, Percent_Identity=34.5360824742268, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=240, Percent_Identity=31.25, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=192, Percent_Identity=31.25, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=196, Percent_Identity=29.5918367346939, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=217, Percent_Identity=27.1889400921659, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=194, Percent_Identity=30.4123711340206, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=194, Percent_Identity=30.4123711340206, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=220, Percent_Identity=29.0909090909091, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=194, Percent_Identity=30.4123711340206, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357171, Length=211, Percent_Identity=32.2274881516588, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24650670, Length=205, Percent_Identity=35.609756097561, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20129971, Length=198, Percent_Identity=34.8484848484849, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24653477, Length=198, Percent_Identity=34.8484848484849, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24653139, Length=243, Percent_Identity=30.0411522633745, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI28571730, Length=145, Percent_Identity=28.9655172413793, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28571729, Length=145, Percent_Identity=28.9655172413793, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24583077, Length=148, Percent_Identity=36.4864864864865, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583079, Length=148, Percent_Identity=36.4864864864865, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921000, Length=148, Percent_Identity=36.4864864864865, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28704; Mature: 28573

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTELVTYELDGNVALIGLNRPEKRNAINEDVVWALHDAVVRAGEEADVGVLFGHGSNFC
CCCEEEEEEECCCEEEEECCCCHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCE
AGLDLKEALARASGEQPRPRKRTRHSWHTVFDGIARGPIPFVAALHGAVVGGGLELATAA
ECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEE
HIRVADESALFGLPEGQRGIFVGGGGTVRIQRVVGYTVMADMMLTGRLLNAQEGEREHIV
EEEEECCCCEECCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHEE
RYVVPQGQALAKARELAALIARNTADTNWRITNVLPRINDLSHDDGLFVEYLTSNIPRPP
EEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCH
EAAERLRDFVEGRAKPLAVPGEKSE
HHHHHHHHHHHCCCCEEECCCCCCC
>Mature Secondary Structure 
STELVTYELDGNVALIGLNRPEKRNAINEDVVWALHDAVVRAGEEADVGVLFGHGSNFC
CCEEEEEEECCCEEEEECCCCHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCE
AGLDLKEALARASGEQPRPRKRTRHSWHTVFDGIARGPIPFVAALHGAVVGGGLELATAA
ECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEE
HIRVADESALFGLPEGQRGIFVGGGGTVRIQRVVGYTVMADMMLTGRLLNAQEGEREHIV
EEEEECCCCEECCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHEE
RYVVPQGQALAKARELAALIARNTADTNWRITNVLPRINDLSHDDGLFVEYLTSNIPRPP
EEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCH
EAAERLRDFVEGRAKPLAVPGEKSE
HHHHHHHHHHHCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA