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The map label for this gene is gcvA [C]

Identifier: 73538599

GI number: 73538599

Start: 1474048

End: 1474938

Strand: Direct

Name: gcvA [C]

Synonym: Reut_B4774

Alternate gene names: 73538599

Gene position: 1474048-1474938 (Clockwise)

Preceding gene: 73538598

Following gene: 73538600

Centisome position: 54.07

GC content: 65.21

Gene sequence:

>891_bases
ATGCGCATTCCACCGATGAAGGCCGTCATCGCCTTCGAAAGCGTTGCACGAACCAAGAGCGTCAGCCGCGCGGCGGAAGA
GCTGGGCCTGACGGCCTCCGCCGTAAGCCATCAGATCAGCAACCTGGAATCGGTGATCGGGCGACCGCTGTTCTTCCGAC
AGGGACGCGGACTGGTGCTGACGCCGGTGGGCGAACAGTACCTGCGCGAGGTGACTGGCGCGCTGGCCGACCTGAATCGT
GCGACCGAACGCGCTTCGAGCCAGCCCGACGTGGAAATCCTCCGCGTGCATTCGAGTCCGAGCTTCGGGCTTATGTGGCT
GCTGCCGCGGCTGGCGTCGTTCCAGGCCGAGCACGGCGACATCCGGCTGAACCTGTCGTGCTCGTATGAGGACATATCGT
TCACGAGCGGCTTCTATGATGTCGACATCCGGCACGGCTACGCGCACTGGCGCGACCTCCAGGTCAAGACGCTGCGCAAG
GAATTCATCGCGCCGCTGGCCTCCGCGGATTACCTGCGCCAGCACGGCATCCAGACGCCGCAAGACCTGCTCGGGCAGCG
CCTGATCTGCTCCGAAACCCCGCTGGTTCAATGGCAGCAATGGTTCGCGCGGTTTGGCATCAACCATGTGCGCAAGACCT
TCGACTTCTCGTTCGACCGGTCCTACATGTCGCTCGAAACTGCGGCGCTTGGCCTTGGCGTGGCCCTCGAAAGCACGCTG
CTGGCTTCGGTGCTGCTGCGCAGCGGCCGGCTGGTGCCCGTGCTGGACCCGACCCACGCAGTGGAGGTCGGCGCGCATCA
CGTCGTCTATCCCGCGCAGAACGGCGACCTGCCCCGCGTCGCGCGGTTCCTTGCCTGGATCGACCGCGAACTGCTCGCCG
ACGACTTGTGA

Upstream 100 bases:

>100_bases
CACGTTGATGCAGCCGTCGCGCGCCGCCTGACGAGCCGCGGAAGTAGAATGCCGCAATCCTCAGGAAACCATCGCCGCCT
TCCGGGCCTGTACTGCAGAC

Downstream 100 bases:

>100_bases
CGGTACCGGCTCGGGCAGGTGCACCAGTGCGCCTGATGAGCCACCTCCCATGCCCGCCGGATCAGCCGATAACTGAAAAT
TTCTCAGGCATAGTTGAGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRIPPMKAVIAFESVARTKSVSRAAEELGLTASAVSHQISNLESVIGRPLFFRQGRGLVLTPVGEQYLREVTGALADLNR
ATERASSQPDVEILRVHSSPSFGLMWLLPRLASFQAEHGDIRLNLSCSYEDISFTSGFYDVDIRHGYAHWRDLQVKTLRK
EFIAPLASADYLRQHGIQTPQDLLGQRLICSETPLVQWQQWFARFGINHVRKTFDFSFDRSYMSLETAALGLGVALESTL
LASVLLRSGRLVPVLDPTHAVEVGAHHVVYPAQNGDLPRVARFLAWIDRELLADDL

Sequences:

>Translated_296_residues
MRIPPMKAVIAFESVARTKSVSRAAEELGLTASAVSHQISNLESVIGRPLFFRQGRGLVLTPVGEQYLREVTGALADLNR
ATERASSQPDVEILRVHSSPSFGLMWLLPRLASFQAEHGDIRLNLSCSYEDISFTSGFYDVDIRHGYAHWRDLQVKTLRK
EFIAPLASADYLRQHGIQTPQDLLGQRLICSETPLVQWQQWFARFGINHVRKTFDFSFDRSYMSLETAALGLGVALESTL
LASVLLRSGRLVPVLDPTHAVEVGAHHVVYPAQNGDLPRVARFLAWIDRELLADDL
>Mature_296_residues
MRIPPMKAVIAFESVARTKSVSRAAEELGLTASAVSHQISNLESVIGRPLFFRQGRGLVLTPVGEQYLREVTGALADLNR
ATERASSQPDVEILRVHSSPSFGLMWLLPRLASFQAEHGDIRLNLSCSYEDISFTSGFYDVDIRHGYAHWRDLQVKTLRK
EFIAPLASADYLRQHGIQTPQDLLGQRLICSETPLVQWQQWFARFGINHVRKTFDFSFDRSYMSLETAALGLGVALESTL
LASVLLRSGRLVPVLDPTHAVEVGAHHVVYPAQNGDLPRVARFLAWIDRELLADDL

Specific function: Regulatory Protein For The Glycine Cleavage System Operon (Gcv). Mediates Activation Of Gcv By Glycine And Repression By Purines. Gcva Is Negatively Autoregulated. Bind To Three Sites Upstream Of The Gcv Promoter. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=287, Percent_Identity=32.7526132404181, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=33.6805555555556, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=28.0276816608997, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=28.956228956229, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1786401, Length=281, Percent_Identity=27.7580071174377, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33104; Mature: 33104

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPPMKAVIAFESVARTKSVSRAAEELGLTASAVSHQISNLESVIGRPLFFRQGRGLVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEE
TPVGEQYLREVTGALADLNRATERASSQPDVEILRVHSSPSFGLMWLLPRLASFQAEHGD
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCC
IRLNLSCSYEDISFTSGFYDVDIRHGYAHWRDLQVKTLRKEFIAPLASADYLRQHGIQTP
EEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
QDLLGQRLICSETPLVQWQQWFARFGINHVRKTFDFSFDRSYMSLETAALGLGVALESTL
HHHHCCCHHCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LASVLLRSGRLVPVLDPTHAVEVGAHHVVYPAQNGDLPRVARFLAWIDRELLADDL
HHHHHHHCCCEEEEECCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIPPMKAVIAFESVARTKSVSRAAEELGLTASAVSHQISNLESVIGRPLFFRQGRGLVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEE
TPVGEQYLREVTGALADLNRATERASSQPDVEILRVHSSPSFGLMWLLPRLASFQAEHGD
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCC
IRLNLSCSYEDISFTSGFYDVDIRHGYAHWRDLQVKTLRKEFIAPLASADYLRQHGIQTP
EEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
QDLLGQRLICSETPLVQWQQWFARFGINHVRKTFDFSFDRSYMSLETAALGLGVALESTL
HHHHCCCHHCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LASVLLRSGRLVPVLDPTHAVEVGAHHVVYPAQNGDLPRVARFLAWIDRELLADDL
HHHHHHHCCCEEEEECCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]