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The map label for this gene is napB [H]

Identifier: 73538589

GI number: 73538589

Start: 1465815

End: 1466321

Strand: Direct

Name: napB [H]

Synonym: Reut_B4764

Alternate gene names: 73538589

Gene position: 1465815-1466321 (Clockwise)

Preceding gene: 73538588

Following gene: 73538590

Centisome position: 53.77

GC content: 63.12

Gene sequence:

>507_bases
ATGAAGCCGAGCCGATCCTGGGCACCGCTGCTGGCCTTGTGTACCTGGCTGCTGGCGGCATTTGCATTTGCCATGTTCGC
GGCCGCGCCCGCCGGCGCGCAGGGCGTGGTCGACGCCATGCGTGGTCCTACGCCCGTGGCCAACGAGACCAAGGCCCCGA
TCCTGTACCCGACCGAGAACAAGGACATCCGGCGCACGCGCAATTACACCATGCAGCCGCCGACGATCCCCCACAAGATC
GACGGCTACCAGCTTGACAAGGACTTCAACCGCTGCATGTTCTGCCATGCACGCACGCGGACCGAGGAGACGCAGGCCAT
TCCGGTCAGCATCACGCATTACATGGATCGCGACAACAATGTGCTGGCCGATGTCTCGCCACGGCGCTATTTCTGCACCC
AATGCCATGTACCGCAGGCCGACGCGAAGCCGCTGGTCGGCAATAACTTCGTCGATGTGGAAACGATGCTCAAGCGCAAG
CCCGGCGCCAAGAGCGCGGGCAAGTAA

Upstream 100 bases:

>100_bases
GACGCGACCTGTCCGATTTCGCTGCAGACCGACTACAAGAAGTGCGCGGTGAAGATCGTCAAGGTGTAGCCGCCCACGCC
AATCCACAAAAGGAGACGCC

Downstream 100 bases:

>100_bases
CCAACAAGACAGGTGCGCGCCATGCTGGATCTCATCAAGCGCTATTGGCGGACCATCAACCGGCCCAGCGCCTATTTCAG
CCTGGGCTTCCTGACGCTGG

Product: periplasmic nitrate reductase subunit NapB

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MKPSRSWAPLLALCTWLLAAFAFAMFAAAPAGAQGVVDAMRGPTPVANETKAPILYPTENKDIRRTRNYTMQPPTIPHKI
DGYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNNVLADVSPRRYFCTQCHVPQADAKPLVGNNFVDVETMLKRK
PGAKSAGK

Sequences:

>Translated_168_residues
MKPSRSWAPLLALCTWLLAAFAFAMFAAAPAGAQGVVDAMRGPTPVANETKAPILYPTENKDIRRTRNYTMQPPTIPHKI
DGYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNNVLADVSPRRYFCTQCHVPQADAKPLVGNNFVDVETMLKRK
PGAKSAGK
>Mature_168_residues
MKPSRSWAPLLALCTWLLAAFAFAMFAAAPAGAQGVVDAMRGPTPVANETKAPILYPTENKDIRRTRNYTMQPPTIPHKI
DGYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNNVLADVSPRRYFCTQCHVPQADAKPLVGNNFVDVETMLKRK
PGAKSAGK

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=139, Percent_Identity=43.1654676258993, Blast_Score=114, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 18754; Mature: 18754

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSRSWAPLLALCTWLLAAFAFAMFAAAPAGAQGVVDAMRGPTPVANETKAPILYPTEN
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEEECCCC
KDIRRTRNYTMQPPTIPHKIDGYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNN
CHHHHHCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCC
VLADVSPRRYFCTQCHVPQADAKPLVGNNFVDVETMLKRKPGAKSAGK
EEEECCCCCEEEEECCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKPSRSWAPLLALCTWLLAAFAFAMFAAAPAGAQGVVDAMRGPTPVANETKAPILYPTEN
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEEECCCC
KDIRRTRNYTMQPPTIPHKIDGYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNN
CHHHHHCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCC
VLADVSPRRYFCTQCHVPQADAKPLVGNNFVDVETMLKRKPGAKSAGK
EEEECCCCCEEEEECCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]