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The map label for this gene is cyaE [H]

Identifier: 73538183

GI number: 73538183

Start: 1013256

End: 1014809

Strand: Direct

Name: cyaE [H]

Synonym: Reut_B4354

Alternate gene names: 73538183

Gene position: 1013256-1014809 (Clockwise)

Preceding gene: 73538182

Following gene: 73538186

Centisome position: 37.17

GC content: 67.95

Gene sequence:

>1554_bases
ATGCGCAAACCTGAGTCCCTGGCCGCGCTGACGCTGTGCGCGCTGACCCTGGCGGGCTGCGCCACGTCGTCGCTCGACCT
CGCGCCGGAGCAGCCGGACCGCCCGTGGCATCCCATCACCACGGCATCGGGCGAGATCGTGCCGGGTGCCGCCGGCCAAC
CCGTGCCAGCGCCGTCCGACTACACGCTGCCGGCAAACCCGGCGCTGGCGGCGGTTTCGCCAGCGGCCACGCTCGACCCC
GCGCATGCCTACACGCTGCCCGAGCTGATCGACCTGGCCGAATCCACCAACCCGCTCACGCGCATTGCGTGGAACGATGC
GCGCAACGCAGCGCTGGCCGCCGGCATTGCCAAGGCGAGCTATTTGCCGTACATCTCGGCCGCCGCCATGGGCGGCTACC
AGAACGGCCACAGCTCCACGTCGACGGTGCTGGGCCCGGTCGGCGCGAATTCATCGAACCACGGTGCGATCTCGGTCCTG
TCGCTGCAATGGCTGCTGTTTGATTTCGGCGAACGTGCGGGCATTGTCGATGCCACGGAGCAGCTATCGGTCGCGGCCAA
TATCGCGTTCAATGCCGTCCACCAGAAGATCATCCACGACGTCAGCGTCGCGTTCTATCGCTACCAGGCGGCGCGCTCGC
GTGCGCGCACCGCGCAGCAGGCGATGGAGAATGCCGATGCAGTGCAGGCCGCGGCCAAGGCGCGCTTTCAGCGAGGCATC
GGCACCGTCGTGGAAGTCGCCCAGGCCACGCAGAACCGCGCGCAGACCAACCTGGCCATGGTCCAGGCGAAGGGTGCGGA
GACCGATGCCTATCTGGCACTCGTGACGGCGGTCGGCATTTCGCCATTGTCCCGGCCGCAGGTCGCGGAGATGCCGTTGC
GGCCGCTCTCCCCCACGCTGCGCAGCTCGGTCGAACAGATCGTCGAAACCGCGATCGCGCGGCGACCCGATGTACTCGCC
GCCTATGCAGTGGAGCGCGCAAGCCAGGCACGCGTGAAATCAGCCGAGGCCGGCTTCATGCCGAAGGTCTTCCTGTCGGC
GTTCACATCGTATGCATCGGGCGGATCGGCCATTACCGCAATTCCGCCCATCGGCCAGCAGCCGCCGACTGTCAACCTGA
ACGGCTACCGCTATGGTTCCAGCGTCTTTCTTGGCGTCACGGTGCCGATCTACGACGGCGGCCTGCGCTCCGCGGTGCTC
GCGCAGGCGCGCAACGACGCCGACAGCGCCTCGACGAAACTGAGCCGCGCGAAAGAAGAGTCCGTACGGCAAGTGGTGGT
CAGCCAGAGCGCGCTGGAATCGAGCCTCGCCGCCTATGACGCGGCCACGACGCTGGCTGATGCGGCCCGGATCACGTATG
ACGCCGCGTTCGCCGCCTACCGCAAGGGCGTTGGGTCGGTGACGGAGGCCAACCTCGCGCAGAATCAGCTGCTGCTTGCG
CAGAATGCCTCGACCGATGCCTACAGCGGCGCGCTGGCCGCCGCGGCGGCGCTGGCGTTGGCCACCGGGTCGATCGGTTC
GATTCGCGACGGTACGACGTGGCCTGCGCGATAG

Upstream 100 bases:

>100_bases
GACGATGTCCGGGCCCGCCAGGCACTGCTCGCCATCGTCGACCAACAACTCGAAACGTCCCATGCCGACGCGTCGGACAC
GGGCTCCCCTTCATCGATTC

Downstream 100 bases:

>100_bases
GCGACATGGGCAGCGCTACGGCCAACGCGCGTTACCGCAGGCCCAGCGTGAACGGCAGCAGAAGCGCATTCAGAAGCAGC
AAGCCATACGCGACCATCAT

Product: Outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MRKPESLAALTLCALTLAGCATSSLDLAPEQPDRPWHPITTASGEIVPGAAGQPVPAPSDYTLPANPALAAVSPAATLDP
AHAYTLPELIDLAESTNPLTRIAWNDARNAALAAGIAKASYLPYISAAAMGGYQNGHSSTSTVLGPVGANSSNHGAISVL
SLQWLLFDFGERAGIVDATEQLSVAANIAFNAVHQKIIHDVSVAFYRYQAARSRARTAQQAMENADAVQAAAKARFQRGI
GTVVEVAQATQNRAQTNLAMVQAKGAETDAYLALVTAVGISPLSRPQVAEMPLRPLSPTLRSSVEQIVETAIARRPDVLA
AYAVERASQARVKSAEAGFMPKVFLSAFTSYASGGSAITAIPPIGQQPPTVNLNGYRYGSSVFLGVTVPIYDGGLRSAVL
AQARNDADSASTKLSRAKEESVRQVVVSQSALESSLAAYDAATTLADAARITYDAAFAAYRKGVGSVTEANLAQNQLLLA
QNASTDAYSGALAAAAALALATGSIGSIRDGTTWPAR

Sequences:

>Translated_517_residues
MRKPESLAALTLCALTLAGCATSSLDLAPEQPDRPWHPITTASGEIVPGAAGQPVPAPSDYTLPANPALAAVSPAATLDP
AHAYTLPELIDLAESTNPLTRIAWNDARNAALAAGIAKASYLPYISAAAMGGYQNGHSSTSTVLGPVGANSSNHGAISVL
SLQWLLFDFGERAGIVDATEQLSVAANIAFNAVHQKIIHDVSVAFYRYQAARSRARTAQQAMENADAVQAAAKARFQRGI
GTVVEVAQATQNRAQTNLAMVQAKGAETDAYLALVTAVGISPLSRPQVAEMPLRPLSPTLRSSVEQIVETAIARRPDVLA
AYAVERASQARVKSAEAGFMPKVFLSAFTSYASGGSAITAIPPIGQQPPTVNLNGYRYGSSVFLGVTVPIYDGGLRSAVL
AQARNDADSASTKLSRAKEESVRQVVVSQSALESSLAAYDAATTLADAARITYDAAFAAYRKGVGSVTEANLAQNQLLLA
QNASTDAYSGALAAAAALALATGSIGSIRDGTTWPAR
>Mature_517_residues
MRKPESLAALTLCALTLAGCATSSLDLAPEQPDRPWHPITTASGEIVPGAAGQPVPAPSDYTLPANPALAAVSPAATLDP
AHAYTLPELIDLAESTNPLTRIAWNDARNAALAAGIAKASYLPYISAAAMGGYQNGHSSTSTVLGPVGANSSNHGAISVL
SLQWLLFDFGERAGIVDATEQLSVAANIAFNAVHQKIIHDVSVAFYRYQAARSRARTAQQAMENADAVQAAAKARFQRGI
GTVVEVAQATQNRAQTNLAMVQAKGAETDAYLALVTAVGISPLSRPQVAEMPLRPLSPTLRSSVEQIVETAIARRPDVLA
AYAVERASQARVKSAEAGFMPKVFLSAFTSYASGGSAITAIPPIGQQPPTVNLNGYRYGSSVFLGVTVPIYDGGLRSAVL
AQARNDADSASTKLSRAKEESVRQVVVSQSALESSLAAYDAATTLADAARITYDAAFAAYRKGVGSVTEANLAQNQLLLA
QNASTDAYSGALAAAAALALATGSIGSIRDGTTWPAR

Specific function: CyaE is necessary for transport of calmodulin-sensitive adenylate cyclase-hemolysin (cyclolysin) [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

None

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53530; Mature: 53530

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPESLAALTLCALTLAGCATSSLDLAPEQPDRPWHPITTASGEIVPGAAGQPVPAPSD
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YTLPANPALAAVSPAATLDPAHAYTLPELIDLAESTNPLTRIAWNDARNAALAAGIAKAS
CCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
YLPYISAAAMGGYQNGHSSTSTVLGPVGANSSNHGAISVLSLQWLLFDFGERAGIVDATE
HCCHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHH
QLSVAANIAFNAVHQKIIHDVSVAFYRYQAARSRARTAQQAMENADAVQAAAKARFQRGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTVVEVAQATQNRAQTNLAMVQAKGAETDAYLALVTAVGISPLSRPQVAEMPLRPLSPTL
HHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHH
RSSVEQIVETAIARRPDVLAAYAVERASQARVKSAEAGFMPKVFLSAFTSYASGGSAITA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEE
IPPIGQQPPTVNLNGYRYGSSVFLGVTVPIYDGGLRSAVLAQARNDADSASTKLSRAKEE
CCCCCCCCCEEECCCEEECCEEEEEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHH
SVRQVVVSQSALESSLAAYDAATTLADAARITYDAAFAAYRKGVGSVTEANLAQNQLLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCEEEEE
QNASTDAYSGALAAAAALALATGSIGSIRDGTTWPAR
ECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKPESLAALTLCALTLAGCATSSLDLAPEQPDRPWHPITTASGEIVPGAAGQPVPAPSD
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YTLPANPALAAVSPAATLDPAHAYTLPELIDLAESTNPLTRIAWNDARNAALAAGIAKAS
CCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
YLPYISAAAMGGYQNGHSSTSTVLGPVGANSSNHGAISVLSLQWLLFDFGERAGIVDATE
HCCHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHH
QLSVAANIAFNAVHQKIIHDVSVAFYRYQAARSRARTAQQAMENADAVQAAAKARFQRGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTVVEVAQATQNRAQTNLAMVQAKGAETDAYLALVTAVGISPLSRPQVAEMPLRPLSPTL
HHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCHHH
RSSVEQIVETAIARRPDVLAAYAVERASQARVKSAEAGFMPKVFLSAFTSYASGGSAITA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEE
IPPIGQQPPTVNLNGYRYGSSVFLGVTVPIYDGGLRSAVLAQARNDADSASTKLSRAKEE
CCCCCCCCCEEECCCEEECCEEEEEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHH
SVRQVVVSQSALESSLAAYDAATTLADAARITYDAAFAAYRKGVGSVTEANLAQNQLLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCEEEEE
QNASTDAYSGALAAAAALALATGSIGSIRDGTTWPAR
ECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2905265; 12910271 [H]