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The map label for this gene is mdtN [H]

Identifier: 73538181

GI number: 73538181

Start: 1010242

End: 1011291

Strand: Direct

Name: mdtN [H]

Synonym: Reut_B4352

Alternate gene names: 73538181

Gene position: 1010242-1011291 (Clockwise)

Preceding gene: 73538180

Following gene: 73538182

Centisome position: 37.06

GC content: 68.19

Gene sequence:

>1050_bases
ATGAAAATGGCCGGCGTACGCAGCAGCCCGAAGCGCGGCAAACTGATCGCGCCGGTGATCGTCCTGCTGGCTGTCCTTCT
TTCCGTCTACGCCTACCAGCGCACCAGCCGCTATCCGTCTTCCGACGACGCCTCGCTCGACGCCGATATCGTCCATGTGG
CATCGCCCGTCGGCGGACGCATTGCGAAGATCCATGTGGCCGAGAACCAGCTGGTCGCCAAGGGCGACGTGTTGTTCGAG
ATCGATCCCGTGCCCTACCAGCTGGTTGTTGCGCAGGCGCGCGCCGATCTCGAACTGGCGCGGGCCGCGCTGGGCACCAG
GCAGCGCACCATCGCCACCGAGCGCGCCACGGCCTCGATCGCTTCCGAGCAGACCAAGCGCGCCGAGCAGAACTACGCGC
TGACTGCCCGCACCGTCGAACGCTTGCGCCCGCTCGTCGCGGACGGCTACGTACCCGCGCAGCAGCTGGACCAGGCGCAA
GTGGCCCAGCGCGACGCCGGCGTATCGCTCAGGCAGGCCAATACGCAGCGCGCCGCTACGGCAGGCGCTGTTGGCAATGA
AGACGATGCCATCGCCACCGTACACGCGCGCGAGGCCGCGCTGGCCATTGCGCAGCGCGCACTTGACGACACCAAGGTCC
GCGCGCCCCACGCCGGGCGCGTGAGCGGCCTGACCGTGCTGTCGGGCGAGGTCGTGATCCCGAATCAGTCCCTGTTCCTG
CTCGTGCATACCGGCGAGTGGTTCGCGGTCGGCAATTTCCGCGAGACGTCGCTGGCCAACATCCGCATCGGCGACTGCGC
CACGGTGTACTCGATGATCGACCGCAGCCAGGCCTTGCGCGGCAAAGTTGACGGCATCGGCATCGGCGTGACGGACACGG
ACCGCATCAACCTGCCCCGCTCGCTGCCTTATGTGCAGCCGTCGGTGAACTGGGTGCGCGTGGCCAAGCGCTTCCCGGTG
CGAATCCGCCTGGAAGACCCGCCCGAGGCACTGGCCCGCGTGGGCGCGAGCGCAACTGTCGAGATCCGGCATGGCGCAGC
CTGCCGCTGA

Upstream 100 bases:

>100_bases
CAGGCTGGGCGGAAATCGTCCGCGCGCAACTGCTGGTCAACACCGCGATCGGCCTGATCATTGCGGAAATCGTCTGGTTA
CTGGGTTCGGGGCACCTCCT

Downstream 100 bases:

>100_bases
CAAGCTGCGCCTGCGCCTTGCCGATATCGGTGCACTGCTCGCGCCGTTGCCAGGCCGCTATGCGGGCGCGACCCGTATCA
CCGTTGGCTGCACGCTGACG

Product: multidrug resistance protein MdtN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKMAGVRSSPKRGKLIAPVIVLLAVLLSVYAYQRTSRYPSSDDASLDADIVHVASPVGGRIAKIHVAENQLVAKGDVLFE
IDPVPYQLVVAQARADLELARAALGTRQRTIATERATASIASEQTKRAEQNYALTARTVERLRPLVADGYVPAQQLDQAQ
VAQRDAGVSLRQANTQRAATAGAVGNEDDAIATVHAREAALAIAQRALDDTKVRAPHAGRVSGLTVLSGEVVIPNQSLFL
LVHTGEWFAVGNFRETSLANIRIGDCATVYSMIDRSQALRGKVDGIGIGVTDTDRINLPRSLPYVQPSVNWVRVAKRFPV
RIRLEDPPEALARVGASATVEIRHGAACR

Sequences:

>Translated_349_residues
MKMAGVRSSPKRGKLIAPVIVLLAVLLSVYAYQRTSRYPSSDDASLDADIVHVASPVGGRIAKIHVAENQLVAKGDVLFE
IDPVPYQLVVAQARADLELARAALGTRQRTIATERATASIASEQTKRAEQNYALTARTVERLRPLVADGYVPAQQLDQAQ
VAQRDAGVSLRQANTQRAATAGAVGNEDDAIATVHAREAALAIAQRALDDTKVRAPHAGRVSGLTVLSGEVVIPNQSLFL
LVHTGEWFAVGNFRETSLANIRIGDCATVYSMIDRSQALRGKVDGIGIGVTDTDRINLPRSLPYVQPSVNWVRVAKRFPV
RIRLEDPPEALARVGASATVEIRHGAACR
>Mature_349_residues
MKMAGVRSSPKRGKLIAPVIVLLAVLLSVYAYQRTSRYPSSDDASLDADIVHVASPVGGRIAKIHVAENQLVAKGDVLFE
IDPVPYQLVVAQARADLELARAALGTRQRTIATERATASIASEQTKRAEQNYALTARTVERLRPLVADGYVPAQQLDQAQ
VAQRDAGVSLRQANTQRAATAGAVGNEDDAIATVHAREAALAIAQRALDDTKVRAPHAGRVSGLTVLSGEVVIPNQSLFL
LVHTGEWFAVGNFRETSLANIRIGDCATVYSMIDRSQALRGKVDGIGIGVTDTDRINLPRSLPYVQPSVNWVRVAKRFPV
RIRLEDPPEALARVGASATVEIRHGAACR

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790519, Length=340, Percent_Identity=36.7647058823529, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI87081951, Length=321, Percent_Identity=28.3489096573209, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1789637, Length=325, Percent_Identity=26.7692307692308, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789041, Length=339, Percent_Identity=26.5486725663717, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788711, Length=349, Percent_Identity=25.5014326647564, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790024, Length=247, Percent_Identity=25.9109311740891, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37512; Mature: 37512

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMAGVRSSPKRGKLIAPVIVLLAVLLSVYAYQRTSRYPSSDDASLDADIVHVASPVGGR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCE
IAKIHVAENQLVAKGDVLFEIDPVPYQLVVAQARADLELARAALGTRQRTIATERATASI
EEEEEEECCCEEECCCEEEEECCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ASEQTKRAEQNYALTARTVERLRPLVADGYVPAQQLDQAQVAQRDAGVSLRQANTQRAAT
HHHHHHHHHHCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHH
AGAVGNEDDAIATVHAREAALAIAQRALDDTKVRAPHAGRVSGLTVLSGEVVIPNQSLFL
CCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCEEECCCCEEE
LVHTGEWFAVGNFRETSLANIRIGDCATVYSMIDRSQALRGKVDGIGIGVTDTDRINLPR
EEECCCEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
SLPYVQPSVNWVRVAKRFPVRIRLEDPPEALARVGASATVEIRHGAACR
CCCCCCCCCHHHHHHHHCCEEEEECCCHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKMAGVRSSPKRGKLIAPVIVLLAVLLSVYAYQRTSRYPSSDDASLDADIVHVASPVGGR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCE
IAKIHVAENQLVAKGDVLFEIDPVPYQLVVAQARADLELARAALGTRQRTIATERATASI
EEEEEEECCCEEECCCEEEEECCCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ASEQTKRAEQNYALTARTVERLRPLVADGYVPAQQLDQAQVAQRDAGVSLRQANTQRAAT
HHHHHHHHHHCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHH
AGAVGNEDDAIATVHAREAALAIAQRALDDTKVRAPHAGRVSGLTVLSGEVVIPNQSLFL
CCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCEEECCCCEEE
LVHTGEWFAVGNFRETSLANIRIGDCATVYSMIDRSQALRGKVDGIGIGVTDTDRINLPR
EEECCCEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
SLPYVQPSVNWVRVAKRFPVRIRLEDPPEALARVGASATVEIRHGAACR
CCCCCCCCCHHHHHHHHCCEEEEECCCHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]