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The map label for this gene is ddh [H]

Identifier: 73537971

GI number: 73537971

Start: 778206

End: 779198

Strand: Reverse

Name: ddh [H]

Synonym: Reut_B4140

Alternate gene names: 73537971

Gene position: 779198-778206 (Counterclockwise)

Preceding gene: 73537972

Following gene: 73537962

Centisome position: 28.58

GC content: 62.24

Gene sequence:

>993_bases
ATGAAAGCTGCCGTATTCAGCGCCAAGCCTTACGACCGAGAGTATCTTGACGCCGCCAATGTTGCCGAAGGCCATCAACT
CGACTTCTTCGACGTCCCGCTCGACATCAACACGGCCGGCCTGGCCGCCGGCCATGGCGCGGTCTGTATCTTCGTCAACG
ATGTGGCGGATGCCGCCGTGCTCGATGCGCTCAGGCGTGGCGGCACGTCGCTGATCGCGCTGCGCTGCACCGGATTCAAC
AATGTCGATCTCGGCGCCGCTGCAAGTCTCGGCATGAAGGTGGTACGCGTCGTCACGTACTCCCCCAATTCGGTGGCTGA
GCATGCCGTAGCCTTGCTGCTGGCCATCAACCGGAAGATCCACCGCGCCTACAACCGTACGCGAGACTTCAATTTCTCGC
TTGATGGCCTGATGGGGTTCGACTTCTGCGGCAAGACCGTGGCCGTGGTCGGCACAGGCAAGATCGGGCGGGTATTTGCC
CGGATCATGCTCGGGTTTGGCTGCGAAGTGATCGGCTATGATGCATATCCCTCACCGGAATTCGAGGCCTTGGGCGGCCG
CTACGCCCAGCCTGGCGAAATCGGCGCCAGTGCCGACATCATTTCCCTGCATTGCCCGCTGACACCGCAGACGCACCACA
TCATCAATGCCGACGCTCTGGCGCGCGCGAAGCCGGGGGCACTGCTTGTCAACACCAGCCGCGGCGGCCTGCTGGATACC
GCGGCCGCCATCGATGCACTGCAGAGCCGCCAGCTGGGTGGGCTGGCGCTGGATGTCTACGAGCAGGAAGCGGATCTTTT
CTTCCGTGATCTCTCCAGCACCATCATTGCCGACGACGTGATGCAGCGGCTGGTCTCGTTTCCCAACGTCATCGTGACCG
GTCACCAGGCCTTTCTCACGCGGGAAGCCGTGTCCACGATCTGCCAGACGACTCTGCGCAGCATGACGGAATTCGAGTCC
GGGCTGGCGCTGACGAATGAGATCAAGGCCTGA

Upstream 100 bases:

>100_bases
ATCCTGTTTGTGGAGATTGTCATGGTCCTGGCTGGCGGGTAGGGCAATCTGGCATGCCTGCCCGAAGCCGGGGGACCTCG
TTCCGATCCGGAGAGAAGCG

Downstream 100 bases:

>100_bases
GGGGTTCGAATCGCTCGATGTTGCCGTTCATGGGCGTAGCGGCGAACAGCCGGATATTGTCGACCAGTTCCTTACTCACT
TTGCAGCGCCTCGCCGTGGT

Product: D-lactate dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKAAVFSAKPYDREYLDAANVAEGHQLDFFDVPLDINTAGLAAGHGAVCIFVNDVADAAVLDALRRGGTSLIALRCTGFN
NVDLGAAASLGMKVVRVVTYSPNSVAEHAVALLLAINRKIHRAYNRTRDFNFSLDGLMGFDFCGKTVAVVGTGKIGRVFA
RIMLGFGCEVIGYDAYPSPEFEALGGRYAQPGEIGASADIISLHCPLTPQTHHIINADALARAKPGALLVNTSRGGLLDT
AAAIDALQSRQLGGLALDVYEQEADLFFRDLSSTIIADDVMQRLVSFPNVIVTGHQAFLTREAVSTICQTTLRSMTEFES
GLALTNEIKA

Sequences:

>Translated_330_residues
MKAAVFSAKPYDREYLDAANVAEGHQLDFFDVPLDINTAGLAAGHGAVCIFVNDVADAAVLDALRRGGTSLIALRCTGFN
NVDLGAAASLGMKVVRVVTYSPNSVAEHAVALLLAINRKIHRAYNRTRDFNFSLDGLMGFDFCGKTVAVVGTGKIGRVFA
RIMLGFGCEVIGYDAYPSPEFEALGGRYAQPGEIGASADIISLHCPLTPQTHHIINADALARAKPGALLVNTSRGGLLDT
AAAIDALQSRQLGGLALDVYEQEADLFFRDLSSTIIADDVMQRLVSFPNVIVTGHQAFLTREAVSTICQTTLRSMTEFES
GLALTNEIKA
>Mature_330_residues
MKAAVFSAKPYDREYLDAANVAEGHQLDFFDVPLDINTAGLAAGHGAVCIFVNDVADAAVLDALRRGGTSLIALRCTGFN
NVDLGAAASLGMKVVRVVTYSPNSVAEHAVALLLAINRKIHRAYNRTRDFNFSLDGLMGFDFCGKTVAVVGTGKIGRVFA
RIMLGFGCEVIGYDAYPSPEFEALGGRYAQPGEIGASADIISLHCPLTPQTHHIINADALARAKPGALLVNTSRGGLLDT
AAAIDALQSRQLGGLALDVYEQEADLFFRDLSSTIIADDVMQRLVSFPNVIVTGHQAFLTREAVSTICQTTLRSMTEFES
GLALTNEIKA

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=255, Percent_Identity=34.5098039215686, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI61743967, Length=236, Percent_Identity=31.3559322033898, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI4557497, Length=236, Percent_Identity=31.3559322033898, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI145580578, Length=236, Percent_Identity=30.0847457627119, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4557499, Length=236, Percent_Identity=30.0847457627119, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI145580575, Length=236, Percent_Identity=30.0847457627119, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI6912396, Length=231, Percent_Identity=29.004329004329, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=51.8292682926829, Blast_Score=354, Evalue=4e-99,
Organism=Escherichia coli, GI1789279, Length=235, Percent_Identity=31.063829787234, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082289, Length=218, Percent_Identity=29.3577981651376, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17532191, Length=259, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI25147481, Length=232, Percent_Identity=29.7413793103448, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322116, Length=238, Percent_Identity=30.2521008403361, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320925, Length=238, Percent_Identity=30.2521008403361, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=195, Percent_Identity=32.3076923076923, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324964, Length=205, Percent_Identity=26.3414634146341, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321253, Length=194, Percent_Identity=29.8969072164948, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921140, Length=227, Percent_Identity=32.5991189427313, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24646446, Length=261, Percent_Identity=32.5670498084291, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24646448, Length=261, Percent_Identity=32.5670498084291, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24646452, Length=261, Percent_Identity=32.5670498084291, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24646450, Length=261, Percent_Identity=32.5670498084291, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI62472511, Length=261, Percent_Identity=32.5670498084291, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28571528, Length=302, Percent_Identity=25.4966887417219, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28574286, Length=266, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585514, Length=259, Percent_Identity=26.6409266409266, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28574282, Length=259, Percent_Identity=26.6409266409266, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45552429, Length=259, Percent_Identity=26.6409266409266, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574284, Length=259, Percent_Identity=26.6409266409266, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551003, Length=259, Percent_Identity=26.6409266409266, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 35154; Mature: 35154

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAAVFSAKPYDREYLDAANVAEGHQLDFFDVPLDINTAGLAAGHGAVCIFVNDVADAAV
CCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCCCCCEECCCCEEEEEECCHHHHHH
LDALRRGGTSLIALRCTGFNNVDLGAAASLGMKVVRVVTYSPNSVAEHAVALLLAINRKI
HHHHHCCCCEEEEEEECCCCCCCCCHHHHHCEEEEEEEEECCCHHHHHHHHHHHHHHHHH
HRAYNRTRDFNFSLDGLMGFDFCGKTVAVVGTGKIGRVFARIMLGFGCEVIGYDAYPSPE
HHHHHCCCCCCCEECCCCCCHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEECCCCCCCC
FEALGGRYAQPGEIGASADIISLHCPLTPQTHHIINADALARAKPGALLVNTSRGGLLDT
HHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEECCHHHHCCCCCEEEEECCCCCCHHH
AAAIDALQSRQLGGLALDVYEQEADLFFRDLSSTIIADDVMQRLVSFPNVIVTGHQAFLT
HHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHH
REAVSTICQTTLRSMTEFESGLALTNEIKA
HHHHHHHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure
MKAAVFSAKPYDREYLDAANVAEGHQLDFFDVPLDINTAGLAAGHGAVCIFVNDVADAAV
CCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCCCCCEECCCCEEEEEECCHHHHHH
LDALRRGGTSLIALRCTGFNNVDLGAAASLGMKVVRVVTYSPNSVAEHAVALLLAINRKI
HHHHHCCCCEEEEEEECCCCCCCCCHHHHHCEEEEEEEEECCCHHHHHHHHHHHHHHHHH
HRAYNRTRDFNFSLDGLMGFDFCGKTVAVVGTGKIGRVFARIMLGFGCEVIGYDAYPSPE
HHHHHCCCCCCCEECCCCCCHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEECCCCCCCC
FEALGGRYAQPGEIGASADIISLHCPLTPQTHHIINADALARAKPGALLVNTSRGGLLDT
HHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEECCHHHHCCCCCEEEEECCCCCCHHH
AAAIDALQSRQLGGLALDVYEQEADLFFRDLSSTIIADDVMQRLVSFPNVIVTGHQAFLT
HHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHH
REAVSTICQTTLRSMTEFESGLALTNEIKA
HHHHHHHHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]