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The map label for this gene is yafC [C]

Identifier: 73537962

GI number: 73537962

Start: 769548

End: 770462

Strand: Reverse

Name: yafC [C]

Synonym: Reut_B4131

Alternate gene names: 73537962

Gene position: 770462-769548 (Counterclockwise)

Preceding gene: 73537971

Following gene: 73537956

Centisome position: 28.26

GC content: 62.73

Gene sequence:

>915_bases
ATGGATCGCTGGACCGAAATCGAGTTGTTCGTACAGACCGCGGAGTTCGGCAGCATCTCGCGCGCCGCCGAAAGCCTCGA
GATGTCCAATGCCGCGGCCAGCCGCTACCTGAGTTCGCTGGAACGGCGGCTGTCGGCGCGCCTGATCGAGCGCAACACGC
GCCGCCTGTACCTGACCCAGGTCGGCGAGCAGTACTTCCGGCGTTGCAAGGAATTGCTGGCGGAGATGAAGGAGGCGGAA
GCCGACGTCAGCGCCGCCGTGCTGGAACCAAGCGGGACACTGACGATCACTGCGTCGCTGTCGTTCGCCATGAAGCATCT
GGCACCGTTGGTGCCGGAGTTCACGACGCGCTATCCGAAGGTGAATGTCAATGTGCTGGCGGCCAATCGCTACTACGACA
TCATGGACAGCGGCACGGACCTGGCCATCCGCACGCGCGAGTTCGAGCCGGACTCTAACCTGACGGTGCGCCGCCTTGCG
CAGACGCGCCGGGTGCTGGCCGCCACGCCGCGCTACCTGGACACGCACGGTATTCCACGCAGCGTCGAAGCGCTGGCGGA
TCATTCCATGCTGATCTATTCGTACGCCAATCGCCCCAATGAACTCTCGTTCACACGCGGCGACGAAAAGCAGGTCGTCA
CGGTGCGCAGCAAGATGGAATCCAACGATGGCCAGGTACTACGCGCCGCGGCGCTGGAGGGCATGGGCATCCTCGTCCAG
CCCAAGTACATCCTTTATGACGATATCGTCGCCGGGCGGCTGATCCCGGTGCTCGATGACTGGGACTTGCCCAGGCTCAC
GATCAATATCGCGTTCCAGAACCGGACGCACCTTCCGGCGCGGACACGCCTGTTTATCGAGCTACTGGTGTCGCACTTCG
AGCGTATGGACTACGAACGCAAGTGGACGTCGTGA

Upstream 100 bases:

>100_bases
AGTCTATACTCCCTCCGAGCGAGAAAAAACGCGGCCCGAGGAAATGTTTCTTCCACCTGGAGAAAAACTCGGCCGGCGTA
GCAGGCGGTGGAGACAACGC

Downstream 100 bases:

>100_bases
CGCGCCCGCTCTGCAAATGCGTGGCGAGCGGGCCGGAGGCTATGGTTGACGTTGACCCGGCTGCATGCCCGCCGCCAGCT
CAGCGGATTTGATGATGGCG

Product: regulatory protein LysR

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDRWTEIELFVQTAEFGSISRAAESLEMSNAAASRYLSSLERRLSARLIERNTRRLYLTQVGEQYFRRCKELLAEMKEAE
ADVSAAVLEPSGTLTITASLSFAMKHLAPLVPEFTTRYPKVNVNVLAANRYYDIMDSGTDLAIRTREFEPDSNLTVRRLA
QTRRVLAATPRYLDTHGIPRSVEALADHSMLIYSYANRPNELSFTRGDEKQVVTVRSKMESNDGQVLRAAALEGMGILVQ
PKYILYDDIVAGRLIPVLDDWDLPRLTINIAFQNRTHLPARTRLFIELLVSHFERMDYERKWTS

Sequences:

>Translated_304_residues
MDRWTEIELFVQTAEFGSISRAAESLEMSNAAASRYLSSLERRLSARLIERNTRRLYLTQVGEQYFRRCKELLAEMKEAE
ADVSAAVLEPSGTLTITASLSFAMKHLAPLVPEFTTRYPKVNVNVLAANRYYDIMDSGTDLAIRTREFEPDSNLTVRRLA
QTRRVLAATPRYLDTHGIPRSVEALADHSMLIYSYANRPNELSFTRGDEKQVVTVRSKMESNDGQVLRAAALEGMGILVQ
PKYILYDDIVAGRLIPVLDDWDLPRLTINIAFQNRTHLPARTRLFIELLVSHFERMDYERKWTS
>Mature_304_residues
MDRWTEIELFVQTAEFGSISRAAESLEMSNAAASRYLSSLERRLSARLIERNTRRLYLTQVGEQYFRRCKELLAEMKEAE
ADVSAAVLEPSGTLTITASLSFAMKHLAPLVPEFTTRYPKVNVNVLAANRYYDIMDSGTDLAIRTREFEPDSNLTVRRLA
QTRRVLAATPRYLDTHGIPRSVEALADHSMLIYSYANRPNELSFTRGDEKQVVTVRSKMESNDGQVLRAAALEGMGILVQ
PKYILYDDIVAGRLIPVLDDWDLPRLTINIAFQNRTHLPARTRLFIELLVSHFERMDYERKWTS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=32.4137931034483, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=29.3515358361775, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789440, Length=257, Percent_Identity=27.6264591439689, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=29.7297297297297, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=25.8620689655172, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=28.125, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1789173, Length=258, Percent_Identity=29.8449612403101, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=27.0833333333333, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788706, Length=261, Percent_Identity=25.2873563218391, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787601, Length=188, Percent_Identity=25, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790011, Length=296, Percent_Identity=22.972972972973, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI157672245, Length=123, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34782; Mature: 34782

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRWTEIELFVQTAEFGSISRAAESLEMSNAAASRYLSSLERRLSARLIERNTRRLYLTQ
CCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
VGEQYFRRCKELLAEMKEAEADVSAAVLEPSGTLTITASLSFAMKHLAPLVPEFTTRYPK
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCHHHHCCCCC
VNVNVLAANRYYDIMDSGTDLAIRTREFEPDSNLTVRRLAQTRRVLAATPRYLDTHGIPR
EEEEEEEECCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCH
SVEALADHSMLIYSYANRPNELSFTRGDEKQVVTVRSKMESNDGQVLRAAALEGMGILVQ
HHHHHHCCCEEEEEECCCCCCCEECCCCCCEEEEEEHHCCCCCCCEEEEHHHCCCEEEEC
PKYILYDDIVAGRLIPVLDDWDLPRLTINIAFQNRTHLPARTRLFIELLVSHFERMDYER
CCEEEEHHHHHCCEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHH
KWTS
CCCC
>Mature Secondary Structure
MDRWTEIELFVQTAEFGSISRAAESLEMSNAAASRYLSSLERRLSARLIERNTRRLYLTQ
CCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
VGEQYFRRCKELLAEMKEAEADVSAAVLEPSGTLTITASLSFAMKHLAPLVPEFTTRYPK
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCHHHHCCCCC
VNVNVLAANRYYDIMDSGTDLAIRTREFEPDSNLTVRRLAQTRRVLAATPRYLDTHGIPR
EEEEEEEECCEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCH
SVEALADHSMLIYSYANRPNELSFTRGDEKQVVTVRSKMESNDGQVLRAAALEGMGILVQ
HHHHHHCCCEEEEEECCCCCCCEECCCCCCEEEEEEHHCCCCCCCEEEEHHHCCCEEEEC
PKYILYDDIVAGRLIPVLDDWDLPRLTINIAFQNRTHLPARTRLFIELLVSHFERMDYER
CCEEEEHHHHHCCEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHH
KWTS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]