Click here to switch to the map view.

The map label for this gene is rutR [H]

Identifier: 73537821

GI number: 73537821

Start: 626392

End: 627093

Strand: Direct

Name: rutR [H]

Synonym: Reut_B3988

Alternate gene names: 73537821

Gene position: 626392-627093 (Clockwise)

Preceding gene: 73537820

Following gene: 73537822

Centisome position: 22.98

GC content: 67.24

Gene sequence:

>702_bases
ATGGACGGCCCCGCGGTTGCCGCGGCGCGCCGCCTGCCGCTGGCGCGCGACGCCAGCGTCGGGCGCATCCGGCAGGAGAA
CGAGGCCATGATCCTGCGCGCGGCCGAGCACGTCTTTGCACGCGCCGGCTTCGCAGGCGCCACCATGGCCGAGATCGCGG
TGCGCGCCGGGGTGCCCAAATCCAACCTCCACTACTACTTCAGAACGAAGCAGGCGCTGTACCGTGCGGTGCTCGCGCAT
ACGCTGCAGCTCTGGCTGTCAGAGACCGAGGCAATCCGCGCCGAGCTGCCTCCGGAGGTCGCGCTCGAACAGTACATCCG
GGCCAAGATGCGCCTGTCGGCGAGCCATCCTGATGCCTCGCGCGTATTCGCCAACGAACTCCTGCACGGCGCACCGGAGA
TCGGCGAAGTCCTGCGCCATGCGCTGCGCGAACTGGTCGAGCACAAGTCGGATGTGATCCGCCACTGGATCGCCACGGGC
CAGATGGCGAGCGTGGATCCGCAACACCTGTTCTTCACGATCTGGGCGGCGACCCAGACCTACGCCGATTTCGAATCGCA
GATCTGCGCTGTGCTCGGTGTGAGCCGACTGGGCCGGCGTGATTTCGAGCTGGCCACCGAACACCTGGTGGCGCTGCTGC
TGCGAGGCTGCGGACTGCTGCCCGTGAGCGCACAGTTGGCGCAGGACATAACAACACCGTAG

Upstream 100 bases:

>100_bases
TTGCGATCGTCACGGCGGCCATCGACGAGGAGCTGAACGAGCACGGCTACATCGTGCCCGGGCTCGGCGATGCCGGGGAC
CGCCTCTACGGCACACGGTG

Downstream 100 bases:

>100_bases
TACTCATCACAGTAGTACTCAAACCAGGGAGGGAGAACTATGAATGCTGGACGCAATCGCAGGACCTGGCTGAAGCTGGC
CGCCGCAGGCGCCGCCGTGG

Product: regulatory protein TetR

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MDGPAVAAARRLPLARDASVGRIRQENEAMILRAAEHVFARAGFAGATMAEIAVRAGVPKSNLHYYFRTKQALYRAVLAH
TLQLWLSETEAIRAELPPEVALEQYIRAKMRLSASHPDASRVFANELLHGAPEIGEVLRHALRELVEHKSDVIRHWIATG
QMASVDPQHLFFTIWAATQTYADFESQICAVLGVSRLGRRDFELATEHLVALLLRGCGLLPVSAQLAQDITTP

Sequences:

>Translated_233_residues
MDGPAVAAARRLPLARDASVGRIRQENEAMILRAAEHVFARAGFAGATMAEIAVRAGVPKSNLHYYFRTKQALYRAVLAH
TLQLWLSETEAIRAELPPEVALEQYIRAKMRLSASHPDASRVFANELLHGAPEIGEVLRHALRELVEHKSDVIRHWIATG
QMASVDPQHLFFTIWAATQTYADFESQICAVLGVSRLGRRDFELATEHLVALLLRGCGLLPVSAQLAQDITTP
>Mature_233_residues
MDGPAVAAARRLPLARDASVGRIRQENEAMILRAAEHVFARAGFAGATMAEIAVRAGVPKSNLHYYFRTKQALYRAVLAH
TLQLWLSETEAIRAELPPEVALEQYIRAKMRLSASHPDASRVFANELLHGAPEIGEVLRHALRELVEHKSDVIRHWIATG
QMASVDPQHLFFTIWAATQTYADFESQICAVLGVSRLGRRDFELATEHLVALLLRGCGLLPVSAQLAQDITTP

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=187, Percent_Identity=42.2459893048128, Blast_Score=158, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 25762; Mature: 25762

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGPAVAAARRLPLARDASVGRIRQENEAMILRAAEHVFARAGFAGATMAEIAVRAGVPK
CCCHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
SNLHYYFRTKQALYRAVLAHTLQLWLSETEAIRAELPPEVALEQYIRAKMRLSASHPDAS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHH
RVFANELLHGAPEIGEVLRHALRELVEHKSDVIRHWIATGQMASVDPQHLFFTIWAATQT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
YADFESQICAVLGVSRLGRRDFELATEHLVALLLRGCGLLPVSAQLAQDITTP
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MDGPAVAAARRLPLARDASVGRIRQENEAMILRAAEHVFARAGFAGATMAEIAVRAGVPK
CCCHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
SNLHYYFRTKQALYRAVLAHTLQLWLSETEAIRAELPPEVALEQYIRAKMRLSASHPDAS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHH
RVFANELLHGAPEIGEVLRHALRELVEHKSDVIRHWIATGQMASVDPQHLFFTIWAATQT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
YADFESQICAVLGVSRLGRRDFELATEHLVALLLRGCGLLPVSAQLAQDITTP
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]