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The map label for this gene is livM [H]

Identifier: 73537614

GI number: 73537614

Start: 380162

End: 381211

Strand: Reverse

Name: livM [H]

Synonym: Reut_B3779

Alternate gene names: 73537614

Gene position: 381211-380162 (Counterclockwise)

Preceding gene: 73537615

Following gene: 73537613

Centisome position: 13.98

GC content: 64.86

Gene sequence:

>1050_bases
ATGACGGCACGCGACAACCGCGCCACGTTCGCTTACGGGCTGCTGCTCGCGGTCCTGGTGGCAGCCCCGTTCCTGGGCGC
CTATCCGGTGCTGGTGATGAAGCTGCTGTGCTTCGCGCTGTTCGCCTGCGCCTTCAACCTGCTGCTGGGCTTCACCGGGC
TGTTGTCGTTCGGCCATGCGGCCTTCTTCGGCGGCGCGGCCTATGCCTGCGGCTATGCGATGAAGTCGCTGCATGTCACG
CCCGAACTCGGCTTGCTGTTGGGCACAGCATTCGGCGCGCTGCTCGGCCTGGCCTTTGGCGCACTCGCTATACGCAGACA
AGGCATCTACTTCGCGATGATCACGCTGGCGCTCGCGCAGATGTTCTACTTCTTCTGCCTGCAGGCGCCCGTCACCGGCG
GCGAAGACGGCTTGCAGGCAGTGCCGCGCGGCAGCCTGTTCGGCGTGGTCTCGCTGGAGCCGGACATGACCACGTATTAC
CTGGTACTGGCCATCACGGCGGCGGCGTTCCTCGGCATCATGCGCATCGTCAATTCGCCGTTCGGGCAGATCCTGCGCGC
GATCAAGGAGAACGAGCCGCGGGCAATCTCGCTTGGCTATGATGCGGATCGTTTCAAGCTGCTGGCGTTCGTGCTGTCAT
CAGCGCTTGCAGGCCTGGCCGGCGCACTCAAGACGCTGGTGCTCGGCTTTGCGACGCTGACGGATGTCCACTGGATGATG
TCGGGCGCCGTGATCCTGATGACGCTCGTTGGGGGCATGGGAACGCTTTCGGGCCCCATCGTCGGCGCTCTCGTCATCGT
TGCACTGGAGAACAAGCTTGGCGATGCCGGCACCGCGCTCGCTTCGCTGACCGGCATCCCCTGGTTCGACTCGCTCGGCG
AATCCGTCAGCATGGTCACCGGGCTGATCTTCGTGGTTTGCGTGCTCACGTTCCGGCGCGGCGTCATGGGCGAAATCGAG
GCGCGGTGGAAAAACCGTCCTGCCTCACGCCCCATCGTTCCGACCACCCGCACAGTCCAATCCGGCGCCGCCGGCACCGG
ACAGGGTTAA

Upstream 100 bases:

>100_bases
CCAAGGTGTTCTACCCCGAGGCTTCGGCCACGGTCGTGTTCGTGGCCATGGTTTGCGTGCTGCTGGTGCGCCCGGCCGGT
CTCTTTGGAAAGGAAGCATG

Downstream 100 bases:

>100_bases
CGCGGATAGTTCCCACATAAAACATCAGTACACTGACCGTTATAGTCAGTGTACTGACAGATAAGTAACGGAGAACCTCC
ATGGCATGGATTGAAATCGA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTARDNRATFAYGLLLAVLVAAPFLGAYPVLVMKLLCFALFACAFNLLLGFTGLLSFGHAAFFGGAAYACGYAMKSLHVT
PELGLLLGTAFGALLGLAFGALAIRRQGIYFAMITLALAQMFYFFCLQAPVTGGEDGLQAVPRGSLFGVVSLEPDMTTYY
LVLAITAAAFLGIMRIVNSPFGQILRAIKENEPRAISLGYDADRFKLLAFVLSSALAGLAGALKTLVLGFATLTDVHWMM
SGAVILMTLVGGMGTLSGPIVGALVIVALENKLGDAGTALASLTGIPWFDSLGESVSMVTGLIFVVCVLTFRRGVMGEIE
ARWKNRPASRPIVPTTRTVQSGAAGTGQG

Sequences:

>Translated_349_residues
MTARDNRATFAYGLLLAVLVAAPFLGAYPVLVMKLLCFALFACAFNLLLGFTGLLSFGHAAFFGGAAYACGYAMKSLHVT
PELGLLLGTAFGALLGLAFGALAIRRQGIYFAMITLALAQMFYFFCLQAPVTGGEDGLQAVPRGSLFGVVSLEPDMTTYY
LVLAITAAAFLGIMRIVNSPFGQILRAIKENEPRAISLGYDADRFKLLAFVLSSALAGLAGALKTLVLGFATLTDVHWMM
SGAVILMTLVGGMGTLSGPIVGALVIVALENKLGDAGTALASLTGIPWFDSLGESVSMVTGLIFVVCVLTFRRGVMGEIE
ARWKNRPASRPIVPTTRTVQSGAAGTGQG
>Mature_348_residues
TARDNRATFAYGLLLAVLVAAPFLGAYPVLVMKLLCFALFACAFNLLLGFTGLLSFGHAAFFGGAAYACGYAMKSLHVTP
ELGLLLGTAFGALLGLAFGALAIRRQGIYFAMITLALAQMFYFFCLQAPVTGGEDGLQAVPRGSLFGVVSLEPDMTTYYL
VLAITAAAFLGIMRIVNSPFGQILRAIKENEPRAISLGYDADRFKLLAFVLSSALAGLAGALKTLVLGFATLTDVHWMMS
GAVILMTLVGGMGTLSGPIVGALVIVALENKLGDAGTALASLTGIPWFDSLGESVSMVTGLIFVVCVLTFRRGVMGEIEA
RWKNRPASRPIVPTTRTVQSGAAGTGQG

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=268, Percent_Identity=26.4925373134328, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 36573; Mature: 36442

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARDNRATFAYGLLLAVLVAAPFLGAYPVLVMKLLCFALFACAFNLLLGFTGLLSFGHA
CCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFFGGAAYACGYAMKSLHVTPELGLLLGTAFGALLGLAFGALAIRRQGIYFAMITLALAQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MFYFFCLQAPVTGGEDGLQAVPRGSLFGVVSLEPDMTTYYLVLAITAAAFLGIMRIVNSP
HHHHHHHHCCCCCCCHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
FGQILRAIKENEPRAISLGYDADRFKLLAFVLSSALAGLAGALKTLVLGFATLTDVHWMM
HHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVILMTLVGGMGTLSGPIVGALVIVALENKLGDAGTALASLTGIPWFDSLGESVSMVT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHH
GLIFVVCVLTFRRGVMGEIEARWKNRPASRPIVPTTRTVQSGAAGTGQG
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure 
TARDNRATFAYGLLLAVLVAAPFLGAYPVLVMKLLCFALFACAFNLLLGFTGLLSFGHA
CCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFFGGAAYACGYAMKSLHVTPELGLLLGTAFGALLGLAFGALAIRRQGIYFAMITLALAQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MFYFFCLQAPVTGGEDGLQAVPRGSLFGVVSLEPDMTTYYLVLAITAAAFLGIMRIVNSP
HHHHHHHHCCCCCCCHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
FGQILRAIKENEPRAISLGYDADRFKLLAFVLSSALAGLAGALKTLVLGFATLTDVHWMM
HHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVILMTLVGGMGTLSGPIVGALVIVALENKLGDAGTALASLTGIPWFDSLGESVSMVT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHH
GLIFVVCVLTFRRGVMGEIEARWKNRPASRPIVPTTRTVQSGAAGTGQG
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]