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The map label for this gene is braD [H]

Identifier: 73537615

GI number: 73537615

Start: 381211

End: 382095

Strand: Reverse

Name: braD [H]

Synonym: Reut_B3780

Alternate gene names: 73537615

Gene position: 382095-381211 (Counterclockwise)

Preceding gene: 73537616

Following gene: 73537614

Centisome position: 14.02

GC content: 63.5

Gene sequence:

>885_bases
ATGGAATTGTTCGGAGTGCCCCTGCCGGCGCTGCTTAGTCAGCTCTTGCTGGGACTGGTGAACGGGTCGTTCTACGCGAT
CCTGAGTCTGGGCCTGGCCGTGATCTTCGGCCTGCTCAACGTGATCAACTTTGCCCATGGTGCGCTGTTCATGCTCGGCG
CAGTGCTGGCGTGGATGGGCATGAACTACCTCGGCCTCAACTATTGGCTGATGCTGCTGCTCGCGCCGCTGGCGACCGGC
GCCATCGGCGTGGTGCTGGAGCGCACCATGTTGCGCCACCTGTACCGGTTCGACCATCTCTACGGACTGCTGCTGACGCT
GGGCATCTGCCTGCTACTGGAAGGATTGCTGCGTTCCGCCTACGGCGTTTCGGGCCTGCCTTACCCAACACCGGAAGCGC
TCACGGGCGCGACGTCGCTGGGTTTCATGGTGCTGCCCAACTACCGCGCGTGGGTCGTACTGGCGTCGCTGGCCGTGTGC
TTTGCGACCTGGTACATGATCGAGAAGACACGGCTCGGTTCCTACCTGCGTGCCGGCACCGAGAATCCGCGCATTGTCGA
AGCGTTCGGCATCAACGTACCGCTGCTGGTGACACTGACCTATGCGTTCGGCGTGGGGCTGGCCGCGCTGGCCGGCGTGC
TCGCCGCCCCGGTAATGCAGGTGTCCCCGCTGATGGGCCAGAACCTGATCATCACCGTCTTTGCGGTCGTGGTGATCGGC
GGCATGGGCTCCATCCTCGGATCGATCTTCACGGGCCTTGGCCTCGGCGTGTTCGAAGGCCTCACCAAGGTGTTCTACCC
CGAGGCTTCGGCCACGGTCGTGTTCGTGGCCATGGTTTGCGTGCTGCTGGTGCGCCCGGCCGGTCTCTTTGGAAAGGAAG
CATGA

Upstream 100 bases:

>100_bases
GATGGAGCACGGCGCCATTGTGGAGGCATTCGAATCGGCGGAGCTGGAACGCAAGATGCCGACGCTTAACGCACTGCTCG
GCGTGTAAGGAGATTTGCCA

Downstream 100 bases:

>100_bases
TGACGGCACGCGACAACCGCGCCACGTTCGCTTACGGGCTGCTGCTCGCGGTCCTGGTGGCAGCCCCGTTCCTGGGCGCC
TATCCGGTGCTGGTGATGAA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MELFGVPLPALLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGAVLAWMGMNYLGLNYWLMLLLAPLATG
AIGVVLERTMLRHLYRFDHLYGLLLTLGICLLLEGLLRSAYGVSGLPYPTPEALTGATSLGFMVLPNYRAWVVLASLAVC
FATWYMIEKTRLGSYLRAGTENPRIVEAFGINVPLLVTLTYAFGVGLAALAGVLAAPVMQVSPLMGQNLIITVFAVVVIG
GMGSILGSIFTGLGLGVFEGLTKVFYPEASATVVFVAMVCVLLVRPAGLFGKEA

Sequences:

>Translated_294_residues
MELFGVPLPALLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGAVLAWMGMNYLGLNYWLMLLLAPLATG
AIGVVLERTMLRHLYRFDHLYGLLLTLGICLLLEGLLRSAYGVSGLPYPTPEALTGATSLGFMVLPNYRAWVVLASLAVC
FATWYMIEKTRLGSYLRAGTENPRIVEAFGINVPLLVTLTYAFGVGLAALAGVLAAPVMQVSPLMGQNLIITVFAVVVIG
GMGSILGSIFTGLGLGVFEGLTKVFYPEASATVVFVAMVCVLLVRPAGLFGKEA
>Mature_294_residues
MELFGVPLPALLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGAVLAWMGMNYLGLNYWLMLLLAPLATG
AIGVVLERTMLRHLYRFDHLYGLLLTLGICLLLEGLLRSAYGVSGLPYPTPEALTGATSLGFMVLPNYRAWVVLASLAVC
FATWYMIEKTRLGSYLRAGTENPRIVEAFGINVPLLVTLTYAFGVGLAALAGVLAAPVMQVSPLMGQNLIITVFAVVVIG
GMGSILGSIFTGLGLGVFEGLTKVFYPEASATVVFVAMVCVLLVRPAGLFGKEA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=295, Percent_Identity=27.4576271186441, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31224; Mature: 31224

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFGVPLPALLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGAVLAWMG
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MNYLGLNYWLMLLLAPLATGAIGVVLERTMLRHLYRFDHLYGLLLTLGICLLLEGLLRSA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGVSGLPYPTPEALTGATSLGFMVLPNYRAWVVLASLAVCFATWYMIEKTRLGSYLRAGT
CCCCCCCCCCCHHHHCHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENPRIVEAFGINVPLLVTLTYAFGVGLAALAGVLAAPVMQVSPLMGQNLIITVFAVVVIG
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GMGSILGSIFTGLGLGVFEGLTKVFYPEASATVVFVAMVCVLLVRPAGLFGKEA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MELFGVPLPALLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGAVLAWMG
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MNYLGLNYWLMLLLAPLATGAIGVVLERTMLRHLYRFDHLYGLLLTLGICLLLEGLLRSA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGVSGLPYPTPEALTGATSLGFMVLPNYRAWVVLASLAVCFATWYMIEKTRLGSYLRAGT
CCCCCCCCCCCHHHHCHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENPRIVEAFGINVPLLVTLTYAFGVGLAALAGVLAAPVMQVSPLMGQNLIITVFAVVVIG
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GMGSILGSIFTGLGLGVFEGLTKVFYPEASATVVFVAMVCVLLVRPAGLFGKEA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]