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The map label for this gene is hcaD [H]

Identifier: 73537612

GI number: 73537612

Start: 378472

End: 379764

Strand: Reverse

Name: hcaD [H]

Synonym: Reut_B3777

Alternate gene names: 73537612

Gene position: 379764-378472 (Counterclockwise)

Preceding gene: 73537613

Following gene: 73537611

Centisome position: 13.93

GC content: 67.75

Gene sequence:

>1293_bases
ATGTCAACGCAAACCCCCATCCTTATCATCGGCGCTGGCCAGGCCGGTGCCATGGCCGCAGCGGAGTTGCGCAAGCTGGG
CCATGCCGAGCGCATTGTCATGGTCGGCGGCGAGCGGCATGCACCGTACGAGCGGCCCCCGCTCTCCAAGGCGGTGCTGG
CCGATGCCAGCCAGCAAGGCCAGGTTGGCGTGCATCCCGCCGGCTTCTATGCGGAGCGCGACATCGAACTGCGCCTATGC
ACCACCGTGAGCGCCATCGACGCCGCGCGCAGCGTTGCCACCTGCACGGACGGCAGCGAGATCCGCTTCGACGCCTGCCT
GCTCGCCACCGGCGGCCGCGCGCGCACGCTGCCCCACTTGCCAGCCGGCACGCCTAACGTCCATTACCTGCGCACGCTGG
ACGACGCAGCTCGCTTGCGCGAAGCGATGCAGGGCGCCGATGAGGTCCTCGTGATCGGCGGCGGTTTCCTGGGGCTGGAA
ACGGCGTCCACTGCAGTCGACCTTGGATTGAAGGTAACCATTCTTGAGAGCGCCGACAGGTTGCTCGCGCGCGCGGCGCC
GGCCGAGTTGAGCGACTGGCTAGCACAGCGCGTCCGCGCACGGGGCGTGGACCTCAGGCTTGGCTGCAGGATTACCGCGA
TCGAGCCGCAGGCGCAAGGCGTGCAGGTACGGCTGGCGGATGGCACCACGTGGCACATACCGGTGCTCGTCGTAGCCATC
GGGCTGACCCCCAACACTGAACTTGCCGCAATCGCAGGGCTTGCACTGCATCCCGGCAACGGCGGCATCCAGATCGATGA
ACAAGGCCGCACCAGCGTGCCGAACATCTATGCCGCCGGCGATTGCGCCAGCCAGTTCCAGCCGCTGTTTGGCGCCGAGA
TGCGCATGGAATCGTGGCAATCGGCCAATGAGCAGGCCCGCATTGCCGCCACGTCCATGCTCGGCTTGCAGGCCGCACTG
GCTGCCACCCCGTGGTTCTGGACCGACCAGTTCGGCTGCAACGTGCAGATGCTCGGCGCCGCCCTTCCCGGCATGCGCTA
CGTATGGCGCGACATGCCGGCCAGCGGCGCGGCAAGCCCGAAGTCCATGCTGCTTGGCACGCTGGACGGCTGCCTGCGCC
ATGCCATCGCCGTCAATGCGGGCGGAGATCTGCGCCAGCTACGCTCGCTCTTCGACTTGCCCGTCAACGAACACCTGGCC
CGCCTGTGCGACGCCGCCCTGCCCCTGCGCCAGATCGTACGCGAGGTACAGGCGGAGATCTCCAGCCGGACCGCGAGCCT
CACCACAAACTGA

Upstream 100 bases:

>100_bases
CCGGCAAGGCCATGTGCGCGCCAGCCAGCACCGACATCCGCACCTACCCGGTCAAGTTCGAAGACAGGCGGGTGTGGGTC
GAGCTGTGACGAGGCAGTAC

Downstream 100 bases:

>100_bases
AAGGAACACCCATGTACCAGACACAAGCGGTGATCCAGCACAAGCTGGGACGCAAGGACACCGAGCTGGACGACTGCCGC
TGGCCCGAAGATGGCCTGCA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: Digoxigenin system ferredoxin--NAD(+) reductase component [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSTQTPILIIGAGQAGAMAAAELRKLGHAERIVMVGGERHAPYERPPLSKAVLADASQQGQVGVHPAGFYAERDIELRLC
TTVSAIDAARSVATCTDGSEIRFDACLLATGGRARTLPHLPAGTPNVHYLRTLDDAARLREAMQGADEVLVIGGGFLGLE
TASTAVDLGLKVTILESADRLLARAAPAELSDWLAQRVRARGVDLRLGCRITAIEPQAQGVQVRLADGTTWHIPVLVVAI
GLTPNTELAAIAGLALHPGNGGIQIDEQGRTSVPNIYAAGDCASQFQPLFGAEMRMESWQSANEQARIAATSMLGLQAAL
AATPWFWTDQFGCNVQMLGAALPGMRYVWRDMPASGAASPKSMLLGTLDGCLRHAIAVNAGGDLRQLRSLFDLPVNEHLA
RLCDAALPLRQIVREVQAEISSRTASLTTN

Sequences:

>Translated_430_residues
MSTQTPILIIGAGQAGAMAAAELRKLGHAERIVMVGGERHAPYERPPLSKAVLADASQQGQVGVHPAGFYAERDIELRLC
TTVSAIDAARSVATCTDGSEIRFDACLLATGGRARTLPHLPAGTPNVHYLRTLDDAARLREAMQGADEVLVIGGGFLGLE
TASTAVDLGLKVTILESADRLLARAAPAELSDWLAQRVRARGVDLRLGCRITAIEPQAQGVQVRLADGTTWHIPVLVVAI
GLTPNTELAAIAGLALHPGNGGIQIDEQGRTSVPNIYAAGDCASQFQPLFGAEMRMESWQSANEQARIAATSMLGLQAAL
AATPWFWTDQFGCNVQMLGAALPGMRYVWRDMPASGAASPKSMLLGTLDGCLRHAIAVNAGGDLRQLRSLFDLPVNEHLA
RLCDAALPLRQIVREVQAEISSRTASLTTN
>Mature_429_residues
STQTPILIIGAGQAGAMAAAELRKLGHAERIVMVGGERHAPYERPPLSKAVLADASQQGQVGVHPAGFYAERDIELRLCT
TVSAIDAARSVATCTDGSEIRFDACLLATGGRARTLPHLPAGTPNVHYLRTLDDAARLREAMQGADEVLVIGGGFLGLET
ASTAVDLGLKVTILESADRLLARAAPAELSDWLAQRVRARGVDLRLGCRITAIEPQAQGVQVRLADGTTWHIPVLVVAIG
LTPNTELAAIAGLALHPGNGGIQIDEQGRTSVPNIYAAGDCASQFQPLFGAEMRMESWQSANEQARIAATSMLGLQAALA
ATPWFWTDQFGCNVQMLGAALPGMRYVWRDMPASGAASPKSMLLGTLDGCLRHAIAVNAGGDLRQLRSLFDLPVNEHLAR
LCDAALPLRQIVREVQAEISSRTASLTTN

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP- dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=342, Percent_Identity=29.8245614035088, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI65787454, Length=342, Percent_Identity=29.8245614035088, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI226437568, Length=342, Percent_Identity=29.8245614035088, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI4757732, Length=360, Percent_Identity=27.2222222222222, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI22202629, Length=360, Percent_Identity=27.2222222222222, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI22202631, Length=164, Percent_Identity=28.6585365853659, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788892, Length=407, Percent_Identity=37.5921375921376, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789065, Length=282, Percent_Identity=30.1418439716312, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786307, Length=199, Percent_Identity=30.1507537688442, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789765, Length=295, Percent_Identity=26.7796610169492, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082354, Length=254, Percent_Identity=26.7716535433071, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=340, Percent_Identity=26.7647058823529, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32564386, Length=342, Percent_Identity=25.7309941520468, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24581020, Length=363, Percent_Identity=27.5482093663912, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28573993, Length=363, Percent_Identity=27.5482093663912, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24585130, Length=337, Percent_Identity=26.7062314540059, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24639257, Length=339, Percent_Identity=27.7286135693215, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281359715, Length=339, Percent_Identity=27.7286135693215, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281359713, Length=339, Percent_Identity=27.7286135693215, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24639250, Length=339, Percent_Identity=27.7286135693215, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI18543267, Length=339, Percent_Identity=27.7286135693215, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24639252, Length=339, Percent_Identity=27.7286135693215, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.18.1.3 [H]

Molecular weight: Translated: 45539; Mature: 45408

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQTPILIIGAGQAGAMAAAELRKLGHAERIVMVGGERHAPYERPPLSKAVLADASQQG
CCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCC
QVGVHPAGFYAERDIELRLCTTVSAIDAARSVATCTDGSEIRFDACLLATGGRARTLPHL
CCCCCCCCCEECCCCEEEEEHHHHHHHHHHHHEECCCCCCEEEEEEEEECCCCCCCCCCC
PAGTPNVHYLRTLDDAARLREAMQGADEVLVIGGGFLGLETASTAVDLGLKVTILESADR
CCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEECCEEECCHHHHHHHCCEEEEEEHHHHH
LLARAAPAELSDWLAQRVRARGVDLRLGCRITAIEPQAQGVQVRLADGTTWHIPVLVVAI
HHHHCCCHHHHHHHHHHHHHCCCCEEECEEEEEECCCCCCEEEEEECCCEEEEEEEEEEE
GLTPNTELAAIAGLALHPGNGGIQIDEQGRTSVPNIYAAGDCASQFQPLFGAEMRMESWQ
CCCCCCCHHHEEEEEEECCCCCEEECCCCCCCCCCEEECCCCHHHCCHHHHHHHHHHHHH
SANEQARIAATSMLGLQAALAATPWFWTDQFGCNVQMLGAALPGMRYVWRDMPASGAASP
CCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEECCCCCCCHHHHHHCCCCCCCCCC
KSMLLGTLDGCLRHAIAVNAGGDLRQLRSLFDLPVNEHLARLCDAALPLRQIVREVQAEI
HHHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SSRTASLTTN
HHCCEEECCC
>Mature Secondary Structure 
STQTPILIIGAGQAGAMAAAELRKLGHAERIVMVGGERHAPYERPPLSKAVLADASQQG
CCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCC
QVGVHPAGFYAERDIELRLCTTVSAIDAARSVATCTDGSEIRFDACLLATGGRARTLPHL
CCCCCCCCCEECCCCEEEEEHHHHHHHHHHHHEECCCCCCEEEEEEEEECCCCCCCCCCC
PAGTPNVHYLRTLDDAARLREAMQGADEVLVIGGGFLGLETASTAVDLGLKVTILESADR
CCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEECCEEECCHHHHHHHCCEEEEEEHHHHH
LLARAAPAELSDWLAQRVRARGVDLRLGCRITAIEPQAQGVQVRLADGTTWHIPVLVVAI
HHHHCCCHHHHHHHHHHHHHCCCCEEECEEEEEECCCCCCEEEEEECCCEEEEEEEEEEE
GLTPNTELAAIAGLALHPGNGGIQIDEQGRTSVPNIYAAGDCASQFQPLFGAEMRMESWQ
CCCCCCCHHHEEEEEEECCCCCEEECCCCCCCCCCEEECCCCHHHCCHHHHHHHHHHHHH
SANEQARIAATSMLGLQAALAATPWFWTDQFGCNVQMLGAALPGMRYVWRDMPASGAASP
CCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEECCCCCCCHHHHHHCCCCCCCCCC
KSMLLGTLDGCLRHAIAVNAGGDLRQLRSLFDLPVNEHLARLCDAALPLRQIVREVQAEI
HHHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SSRTASLTTN
HHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]