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The map label for this gene is bphA1 [H]

Identifier: 73537611

GI number: 73537611

Start: 377171

End: 378460

Strand: Reverse

Name: bphA1 [H]

Synonym: Reut_B3776

Alternate gene names: 73537611

Gene position: 378460-377171 (Counterclockwise)

Preceding gene: 73537612

Following gene: 73537610

Centisome position: 13.88

GC content: 61.47

Gene sequence:

>1290_bases
ATGTACCAGACACAAGCGGTGATCCAGCACAAGCTGGGACGCAAGGACACCGAGCTGGACGACTGCCGCTGGCCCGAAGA
TGGCCTGCACTTCATCCCGGACTGGGTCTACACGAGCCAGTCCGTGTATGACCTCGAACTCGAAAAGATCTTCCGCGGCC
GCTCGTGGAACTACGTCGCGCTCGAAGCGGAAATCCCCAATGCGGGCGACTACAAGCGCTCCTATGTCGGCGCCACGCCG
GTTGTGGTGTCGCGCGCCGAAGATGGCTCGATCAATGTGTTCGAAAACCGCTGCGCGCACCGCGGCGCGGAGTTCTGCCG
CCACAGCCAGGGCAATACGAAGGAGTTCGTCTGCCCCTATCACCAATGGTCCTATGACCTCAAAGGCAACCTTCAGGGCG
TGCCGTTCAAGCGTGGCGTGAACCGCGCGGGCGGCATGCCGAAGGACTTCCGCAACGAGGACCACGGGCTCGTAAAGCTG
AACGTGGCCACGCGCAACGGCGTGATCTTCGCCTCCTATGCGGACAACATGGAAAGCCTGGAGGAGTACATGACGCCGGA
GATCCTCAAGGACTTCGACGTCGTGTTCAACGGCAAGCCGCTCAAGGTGCTCGGGTACTACAAGAATGAGCTGCCGTGCA
ACTGGAAGATGTACCACGAGAACCTGAAGGATCCCTACCACGCCACGCTGCTGCACTCGTTCCTCGTGGTGTTCGGCCTG
CTCGTCGCGGGCAACGAGTCCGCGATGATCGCCGACTCCGTGCATGGCCGCCACGGCACCATGGCATCGGCCAAGAAGGA
AGACAAGTACGCGGCCGTCAGCGACGAAAACAAGAAGGAGATGCGCTCTTACCACGAAGGCATGCGGCTCGAAGACGAGC
GTTTCCTGGAGTACGTGAAGGAATTCGACTCGCCGTGGTCCGTCACCATGCAGACCATCTGGCCGAACCTGATCGTGCAG
CGCGAGATGAACACGCTGGGCGTGCGCCACATCGTCCCCAACGGCCCCGACAGCATGCTGATGCTGTGGACCATGTTTGG
CTACGAAGACGACACCGAGGAAATGACCCAGCACCGCCTGCGCCAGGGCAACCTGATGGGGCCGGCCGGCTTCCTCGGGC
TGGAGGACAACGAGGCGATGAAGTTCGTGCAGGAAGGCGTGCGCCGCTCGACCACGGACGTCAACGTGCTCAAGCTCGAT
GCCAACCATGTGGGCACGTCGAATTCGCTGATCTCGGAATCGGCGATCCGGGCCATGTACCAGTACTACCGCGGCGTGAT
GGGGTTCTGA

Upstream 100 bases:

>100_bases
TGTGCGACGCCGCCCTGCCCCTGCGCCAGATCGTACGCGAGGTACAGGCGGAGATCTCCAGCCGGACCGCGAGCCTCACC
ACAAACTGAAAGGAACACCC

Downstream 100 bases:

>100_bases
ACGATGAAACCGATGTACTACGCTGTCTATCGCGAAAGCAAGCTTGGCGCGGCGCGCGCCATCGAACTGCGGCTCGAGAT
CGAGAATTTCCACGCGGAAT

Product: ring hydroxylating dioxygenase subunit alpha

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MYQTQAVIQHKLGRKDTELDDCRWPEDGLHFIPDWVYTSQSVYDLELEKIFRGRSWNYVALEAEIPNAGDYKRSYVGATP
VVVSRAEDGSINVFENRCAHRGAEFCRHSQGNTKEFVCPYHQWSYDLKGNLQGVPFKRGVNRAGGMPKDFRNEDHGLVKL
NVATRNGVIFASYADNMESLEEYMTPEILKDFDVVFNGKPLKVLGYYKNELPCNWKMYHENLKDPYHATLLHSFLVVFGL
LVAGNESAMIADSVHGRHGTMASAKKEDKYAAVSDENKKEMRSYHEGMRLEDERFLEYVKEFDSPWSVTMQTIWPNLIVQ
REMNTLGVRHIVPNGPDSMLMLWTMFGYEDDTEEMTQHRLRQGNLMGPAGFLGLEDNEAMKFVQEGVRRSTTDVNVLKLD
ANHVGTSNSLISESAIRAMYQYYRGVMGF

Sequences:

>Translated_429_residues
MYQTQAVIQHKLGRKDTELDDCRWPEDGLHFIPDWVYTSQSVYDLELEKIFRGRSWNYVALEAEIPNAGDYKRSYVGATP
VVVSRAEDGSINVFENRCAHRGAEFCRHSQGNTKEFVCPYHQWSYDLKGNLQGVPFKRGVNRAGGMPKDFRNEDHGLVKL
NVATRNGVIFASYADNMESLEEYMTPEILKDFDVVFNGKPLKVLGYYKNELPCNWKMYHENLKDPYHATLLHSFLVVFGL
LVAGNESAMIADSVHGRHGTMASAKKEDKYAAVSDENKKEMRSYHEGMRLEDERFLEYVKEFDSPWSVTMQTIWPNLIVQ
REMNTLGVRHIVPNGPDSMLMLWTMFGYEDDTEEMTQHRLRQGNLMGPAGFLGLEDNEAMKFVQEGVRRSTTDVNVLKLD
ANHVGTSNSLISESAIRAMYQYYRGVMGF
>Mature_429_residues
MYQTQAVIQHKLGRKDTELDDCRWPEDGLHFIPDWVYTSQSVYDLELEKIFRGRSWNYVALEAEIPNAGDYKRSYVGATP
VVVSRAEDGSINVFENRCAHRGAEFCRHSQGNTKEFVCPYHQWSYDLKGNLQGVPFKRGVNRAGGMPKDFRNEDHGLVKL
NVATRNGVIFASYADNMESLEEYMTPEILKDFDVVFNGKPLKVLGYYKNELPCNWKMYHENLKDPYHATLLHSFLVVFGL
LVAGNESAMIADSVHGRHGTMASAKKEDKYAAVSDENKKEMRSYHEGMRLEDERFLEYVKEFDSPWSVTMQTIWPNLIVQ
REMNTLGVRHIVPNGPDSMLMLWTMFGYEDDTEEMTQHRLRQGNLMGPAGFLGLEDNEAMKFVQEGVRRSTTDVNVLKLD
ANHVGTSNSLISESAIRAMYQYYRGVMGF

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=427, Percent_Identity=29.2740046838407, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788103, Length=202, Percent_Identity=28.2178217821782, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 49215; Mature: 49215

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQTQAVIQHKLGRKDTELDDCRWPEDGLHFIPDWVYTSQSVYDLELEKIFRGRSWNYVA
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCHHCCCCCEEHHHHHHHHCCCCCCEEE
LEAEIPNAGDYKRSYVGATPVVVSRAEDGSINVFENRCAHRGAEFCRHSQGNTKEFVCPY
EEEECCCCCCCHHHHCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEECH
HQWSYDLKGNLQGVPFKRGVNRAGGMPKDFRNEDHGLVKLNVATRNGVIFASYADNMESL
HHCCCCCCCCCCCCCHHHCCCCCCCCCHHHCCCCCCEEEEEEEECCCEEEEECCCCHHHH
EEYMTPEILKDFDVVFNGKPLKVLGYYKNELPCNWKMYHENLKDPYHATLLHSFLVVFGL
HHHCCHHHHHHHHHEECCCCEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
LVAGNESAMIADSVHGRHGTMASAKKEDKYAAVSDENKKEMRSYHEGMRLEDERFLEYVK
HHCCCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCHHHHHHHHH
EFDSPWSVTMQTIWPNLIVQREMNTLGVRHIVPNGPDSMLMLWTMFGYEDDTEEMTQHRL
HHCCCCEEEHHHHCCHHHHHHHHHHCCEEEECCCCCCHHEEEEHHHCCCCCHHHHHHHHH
RQGNLMGPAGFLGLEDNEAMKFVQEGVRRSTTDVNVLKLDANHVGTSNSLISESAIRAMY
HCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
QYYRGVMGF
HHHHHHCCC
>Mature Secondary Structure
MYQTQAVIQHKLGRKDTELDDCRWPEDGLHFIPDWVYTSQSVYDLELEKIFRGRSWNYVA
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCHHCCCCCEEHHHHHHHHCCCCCCEEE
LEAEIPNAGDYKRSYVGATPVVVSRAEDGSINVFENRCAHRGAEFCRHSQGNTKEFVCPY
EEEECCCCCCCHHHHCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEECH
HQWSYDLKGNLQGVPFKRGVNRAGGMPKDFRNEDHGLVKLNVATRNGVIFASYADNMESL
HHCCCCCCCCCCCCCHHHCCCCCCCCCHHHCCCCCCEEEEEEEECCCEEEEECCCCHHHH
EEYMTPEILKDFDVVFNGKPLKVLGYYKNELPCNWKMYHENLKDPYHATLLHSFLVVFGL
HHHCCHHHHHHHHHEECCCCEEEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
LVAGNESAMIADSVHGRHGTMASAKKEDKYAAVSDENKKEMRSYHEGMRLEDERFLEYVK
HHCCCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCHHHHHHHHH
EFDSPWSVTMQTIWPNLIVQREMNTLGVRHIVPNGPDSMLMLWTMFGYEDDTEEMTQHRL
HHCCCCEEEHHHHCCHHHHHHHHHHCCEEEECCCCCCHHEEEEHHHCCCCCHHHHHHHHH
RQGNLMGPAGFLGLEDNEAMKFVQEGVRRSTTDVNVLKLDANHVGTSNSLISESAIRAMY
HCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
QYYRGVMGF
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8048958 [H]