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The map label for this gene is nifA [H]

Identifier: 73537562

GI number: 73537562

Start: 319082

End: 320593

Strand: Reverse

Name: nifA [H]

Synonym: Reut_B3727

Alternate gene names: 73537562

Gene position: 320593-319082 (Counterclockwise)

Preceding gene: 73537565

Following gene: 73537558

Centisome position: 11.76

GC content: 68.19

Gene sequence:

>1512_bases
ATGGAAACACTCAGTGACGCAGGAGACAACGTGCTGGGCAACTACGACTCCGTGGCGCGCCGCGCGATGGAATCGCTGTT
CCGCACGTTCGAGAATTTCAGCGAGGGCACGATCGTCGTCGACGTCAACGGCCGCGTCGTGTGGATCAACAAGCGTTATG
CGGCGCGCTTCGGCTTTGCCGATCCGCAGGAAGCGGTCGGCCTCGATTGCGAGCGGGTGATTCCCAACAGCCTGATGCGC
GAGGTGGTGGACACCGGCAAGCCGATCCTGCTCGACATCATGGAGGCCGGGCGCGAGCCGATGATCGTGATGCGGCTGCC
GATCAAGGACGACGACGGCAATACCGTGGGCGCAGTGGGCTTCGCGCTATTCGACGAACTCAAGGCGCTGACGCCACTGC
TGTCGCACTACACGCGGGCCCGCGACGAACTGGCCGCCACGCGCCGCACGCTGGAGCAGACGCGCCGCGCCAAGTACACC
TTCGCGAGCTTCGTCGGCAACTCGCCCGCGACGCTGGAGGTCAAGCGCCTGGCGCGCCGGGCCGCGCAGGTCGATGCACC
GGTGCTGCTGCTCGGCGAGACTGGCACGGGCAAGGAACTGCTCGCGCATGCCATCCATGCCGGGTCATTGCGTGCCGACC
GTCCGCTGGTCACCGTCAACGTCGCGGCAATTCCCGAAGCGCTGCTCGAAGTAGAGTTCTTCGGCGCCGCGCCGGGCGCC
TACACCGGCGCGGAACGCAAGGGACGCGTCGGCAAGTTCGAGCTGGCCGATGGCGGCACGCTGTTTCTCGACGAGATCGG
CGACATGCCGCTTGCGCTGCAGAGCAAACTGCTGCGCGCGTTGCAGGACAAGGAGATCGAGCCGATCGGCTCGAACCGCA
TCATCCGCTCGGATGTGCGCATCATTGCCGCGACCTCGGCCGACCTGCCTGCGCTGGTGGAAGCCGGCAAGTTCCGTGCG
GACCTCTACTACCGGCTCAATGTCGTGCCGATCTGCCTGCCGCCGCTGCGCCAGCGCCAGGCGGACCTGCTGCCGCTCGC
CTACGCGATCCTCGAAGAAATCTGCCGGCGCACGGAGCGCGACGCGCCCGTGCTGTCCGACTGTGCGCTGAGCCTCATGG
GCGAACACGACTGGCCGGGCAATGTGCGCGAACTGCGCAACACGCTGGAGCGCCTGGTGATGCTGCACGATGGCGGCGCC
GTCGATGCATCGATGCTGGCCCCGCTGCTCGGCCGGCGAGCCGTGACGGCGCCGGCTGCCGTGGCCGCTTCGGCGCCATC
CCCCGACGCGATGACCGCTACAGAGCCGGTGCTGCCCGCGGATCCACCCGCCTACGCGGACGCCATGGCGCAATACGAGG
CCGCCTACCTGTCACAAGCGCTGGCTGCAAGCGGAAGCAATGTGCCGCGTGCGGCCAGGGCCATCGGCATCAGCCGCGCC
GCGTTCTACAAGAAGCTCGTGGCGCACCGCGCGGCCGGGCGCATCGCACCGGCCGGCACGGCGGAGTCCTGA

Upstream 100 bases:

>100_bases
GTCTACCTGGGGACGTCTTAATTCGGAGACACGAGCGCAAACCCATGCACCATACGCGCTCGATTGTGAGACTATCCGTC
TCCAAATCGAGAATAATCAA

Downstream 100 bases:

>100_bases
GCGCGGTCAGTGGACGGGATGGCTTGTTGCGATGGTCTGGCCGATCATCGTCGCCGTCGCGGCCGACAGCGTCCAGCCCA
GATGGCCGTGCCCTGTGTTG

Product: Fis-type helix-turn-helix domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
METLSDAGDNVLGNYDSVARRAMESLFRTFENFSEGTIVVDVNGRVVWINKRYAARFGFADPQEAVGLDCERVIPNSLMR
EVVDTGKPILLDIMEAGREPMIVMRLPIKDDDGNTVGAVGFALFDELKALTPLLSHYTRARDELAATRRTLEQTRRAKYT
FASFVGNSPATLEVKRLARRAAQVDAPVLLLGETGTGKELLAHAIHAGSLRADRPLVTVNVAAIPEALLEVEFFGAAPGA
YTGAERKGRVGKFELADGGTLFLDEIGDMPLALQSKLLRALQDKEIEPIGSNRIIRSDVRIIAATSADLPALVEAGKFRA
DLYYRLNVVPICLPPLRQRQADLLPLAYAILEEICRRTERDAPVLSDCALSLMGEHDWPGNVRELRNTLERLVMLHDGGA
VDASMLAPLLGRRAVTAPAAVAASAPSPDAMTATEPVLPADPPAYADAMAQYEAAYLSQALAASGSNVPRAARAIGISRA
AFYKKLVAHRAAGRIAPAGTAES

Sequences:

>Translated_503_residues
METLSDAGDNVLGNYDSVARRAMESLFRTFENFSEGTIVVDVNGRVVWINKRYAARFGFADPQEAVGLDCERVIPNSLMR
EVVDTGKPILLDIMEAGREPMIVMRLPIKDDDGNTVGAVGFALFDELKALTPLLSHYTRARDELAATRRTLEQTRRAKYT
FASFVGNSPATLEVKRLARRAAQVDAPVLLLGETGTGKELLAHAIHAGSLRADRPLVTVNVAAIPEALLEVEFFGAAPGA
YTGAERKGRVGKFELADGGTLFLDEIGDMPLALQSKLLRALQDKEIEPIGSNRIIRSDVRIIAATSADLPALVEAGKFRA
DLYYRLNVVPICLPPLRQRQADLLPLAYAILEEICRRTERDAPVLSDCALSLMGEHDWPGNVRELRNTLERLVMLHDGGA
VDASMLAPLLGRRAVTAPAAVAASAPSPDAMTATEPVLPADPPAYADAMAQYEAAYLSQALAASGSNVPRAARAIGISRA
AFYKKLVAHRAAGRIAPAGTAES
>Mature_503_residues
METLSDAGDNVLGNYDSVARRAMESLFRTFENFSEGTIVVDVNGRVVWINKRYAARFGFADPQEAVGLDCERVIPNSLMR
EVVDTGKPILLDIMEAGREPMIVMRLPIKDDDGNTVGAVGFALFDELKALTPLLSHYTRARDELAATRRTLEQTRRAKYT
FASFVGNSPATLEVKRLARRAAQVDAPVLLLGETGTGKELLAHAIHAGSLRADRPLVTVNVAAIPEALLEVEFFGAAPGA
YTGAERKGRVGKFELADGGTLFLDEIGDMPLALQSKLLRALQDKEIEPIGSNRIIRSDVRIIAATSADLPALVEAGKFRA
DLYYRLNVVPICLPPLRQRQADLLPLAYAILEEICRRTERDAPVLSDCALSLMGEHDWPGNVRELRNTLERLVMLHDGGA
VDASMLAPLLGRRAVTAPAAVAASAPSPDAMTATEPVLPADPPAYADAMAQYEAAYLSQALAASGSNVPRAARAIGISRA
AFYKKLVAHRAAGRIAPAGTAES

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=338, Percent_Identity=40.8284023668639, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789087, Length=254, Percent_Identity=50.3937007874016, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=41.5384615384615, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1790437, Length=323, Percent_Identity=43.9628482972136, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1788905, Length=327, Percent_Identity=41.8960244648318, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI87082117, Length=268, Percent_Identity=46.2686567164179, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1786524, Length=356, Percent_Identity=38.2022471910112, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1790299, Length=332, Percent_Identity=40.0602409638554, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=49.7854077253219, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI87081872, Length=332, Percent_Identity=36.7469879518072, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1787583, Length=238, Percent_Identity=40.7563025210084, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87081858, Length=337, Percent_Identity=32.9376854599407, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789828, Length=255, Percent_Identity=34.9019607843137, Blast_Score=132, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54189; Mature: 54189

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLSDAGDNVLGNYDSVARRAMESLFRTFENFSEGTIVVDVNGRVVWINKRYAARFGFA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECHHHHHCCCC
DPQEAVGLDCERVIPNSLMREVVDTGKPILLDIMEAGREPMIVMRLPIKDDDGNTVGAVG
CCHHHHCCCHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCEEEEEECEECCCCCCHHHHH
FALFDELKALTPLLSHYTRARDELAATRRTLEQTRRAKYTFASFVGNSPATLEVKRLARR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AAQVDAPVLLLGETGTGKELLAHAIHAGSLRADRPLVTVNVAAIPEALLEVEFFGAAPGA
HHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHEECCCCCC
YTGAERKGRVGKFELADGGTLFLDEIGDMPLALQSKLLRALQDKEIEPIGSNRIIRSDVR
CCCCCCCCCCCEEEECCCCEEEEHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCE
IIAATSADLPALVEAGKFRADLYYRLNVVPICLPPLRQRQADLLPLAYAILEEICRRTER
EEEECCCCCHHHHHCCCHHEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
DAPVLSDCALSLMGEHDWPGNVRELRNTLERLVMLHDGGAVDASMLAPLLGRRAVTAPAA
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHH
VAASAPSPDAMTATEPVLPADPPAYADAMAQYEAAYLSQALAASGSNVPRAARAIGISRA
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AFYKKLVAHRAAGRIAPAGTAES
HHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
METLSDAGDNVLGNYDSVARRAMESLFRTFENFSEGTIVVDVNGRVVWINKRYAARFGFA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECHHHHHCCCC
DPQEAVGLDCERVIPNSLMREVVDTGKPILLDIMEAGREPMIVMRLPIKDDDGNTVGAVG
CCHHHHCCCHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCEEEEEECEECCCCCCHHHHH
FALFDELKALTPLLSHYTRARDELAATRRTLEQTRRAKYTFASFVGNSPATLEVKRLARR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AAQVDAPVLLLGETGTGKELLAHAIHAGSLRADRPLVTVNVAAIPEALLEVEFFGAAPGA
HHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHEECCCCCC
YTGAERKGRVGKFELADGGTLFLDEIGDMPLALQSKLLRALQDKEIEPIGSNRIIRSDVR
CCCCCCCCCCCEEEECCCCEEEEHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCE
IIAATSADLPALVEAGKFRADLYYRLNVVPICLPPLRQRQADLLPLAYAILEEICRRTER
EEEECCCCCHHHHHCCCHHEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
DAPVLSDCALSLMGEHDWPGNVRELRNTLERLVMLHDGGAVDASMLAPLLGRRAVTAPAA
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHH
VAASAPSPDAMTATEPVLPADPPAYADAMAQYEAAYLSQALAASGSNVPRAARAIGISRA
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AFYKKLVAHRAAGRIAPAGTAES
HHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2840552; 2450865 [H]