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The map label for this gene is glnH [H]

Identifier: 73537534

GI number: 73537534

Start: 284013

End: 284759

Strand: Reverse

Name: glnH [H]

Synonym: Reut_B3699

Alternate gene names: 73537534

Gene position: 284759-284013 (Counterclockwise)

Preceding gene: 73537535

Following gene: 73537533

Centisome position: 10.45

GC content: 58.37

Gene sequence:

>747_bases
ATGAAGCTCTTTGCCAAGTTCGCCTCGGCTGTGTGCCTTGCCGCCAGTCTTGTCGCCCACGCCGCACATGCCGAGCAGCT
GCTCGTGGCCGTAGACACCGCCTTCGTCCCGTTCGAGTTCAAGCAAGGCGACAAGTATGTCGGCTTCGACATTGATCTAT
GGGATGCCATCGCCAGGGATCTGAAGCTCGACTACAAGCTGCAGCCAATGGATTTCAATGGCATCCTGCCGGCGCTCCAG
ACCCGCAATGTCGACGTGGCGCTGGCTGGCATCACCATCAAGGAAGACCGCAAGAAAGTCATCGATTTCTCCGATGGCTA
CTATGACAGCGGCTTCATGCTGATGGTGCCGAGTTCCAGCACGATCAAGGGCCCGAACGAGCTGAAGGGCAAATCCCTGG
CGCTCAAGACCGGTACTTCCGCTGCCGACTACGCCAAATCGAACTTCGCCGGAACCGATCTGCGGCTCTTCCCGAATATT
GACAACGCTTACCTTGAACTGAGGACGGGACGCGTGGATGCGGCCATGCACGACACACCGAATGTCCTGTACTACATCCA
CACCGCCGGCAAAGGACAAGTCAAGGCAGTCGGGCCGCAGATGATGGCGCAGAAGTACGGCATCGGATTTCCGAAGGGCA
GCCCACTGGTTCCCAAGGTCAACGAGGCGCTGGCAAAGATTCGGGCCGACGGCCGGTACGCCGCTATCTACAAGAAGTGG
TTCGGTGTCGAACCGGCAAACATGTGA

Upstream 100 bases:

>100_bases
ATTTGCTTTACGAGCACTTACGCAAATGTAGTTGCCTGAAAATGTGCGATACGCACCTTGCCTGATTCATGTTGGTGAAC
ACGTCTGAAAGGATTTCCGT

Downstream 100 bases:

>100_bases
TCCATCACCGAAGCGGAGAACCTAGATGGAGTTCGATTTTTCGGTGATTACCGACGCCCTCCCGGCCCTGCTCCAGGGCG
CGAGGCTGACGGTCGCCATT

Product: glutamine ABC transporter periplasmic protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKLFAKFASAVCLAASLVAHAAHAEQLLVAVDTAFVPFEFKQGDKYVGFDIDLWDAIARDLKLDYKLQPMDFNGILPALQ
TRNVDVALAGITIKEDRKKVIDFSDGYYDSGFMLMVPSSSTIKGPNELKGKSLALKTGTSAADYAKSNFAGTDLRLFPNI
DNAYLELRTGRVDAAMHDTPNVLYYIHTAGKGQVKAVGPQMMAQKYGIGFPKGSPLVPKVNEALAKIRADGRYAAIYKKW
FGVEPANM

Sequences:

>Translated_248_residues
MKLFAKFASAVCLAASLVAHAAHAEQLLVAVDTAFVPFEFKQGDKYVGFDIDLWDAIARDLKLDYKLQPMDFNGILPALQ
TRNVDVALAGITIKEDRKKVIDFSDGYYDSGFMLMVPSSSTIKGPNELKGKSLALKTGTSAADYAKSNFAGTDLRLFPNI
DNAYLELRTGRVDAAMHDTPNVLYYIHTAGKGQVKAVGPQMMAQKYGIGFPKGSPLVPKVNEALAKIRADGRYAAIYKKW
FGVEPANM
>Mature_248_residues
MKLFAKFASAVCLAASLVAHAAHAEQLLVAVDTAFVPFEFKQGDKYVGFDIDLWDAIARDLKLDYKLQPMDFNGILPALQ
TRNVDVALAGITIKEDRKKVIDFSDGYYDSGFMLMVPSSSTIKGPNELKGKSLALKTGTSAADYAKSNFAGTDLRLFPNI
DNAYLELRTGRVDAAMHDTPNVLYYIHTAGKGQVKAVGPQMMAQKYGIGFPKGSPLVPKVNEALAKIRADGRYAAIYKKW
FGVEPANM

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=239, Percent_Identity=63.1799163179916, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1788228, Length=223, Percent_Identity=32.2869955156951, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787085, Length=234, Percent_Identity=29.9145299145299, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787088, Length=240, Percent_Identity=30, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788649, Length=244, Percent_Identity=29.9180327868852, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788648, Length=230, Percent_Identity=29.5652173913043, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27094; Mature: 27094

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFAKFASAVCLAASLVAHAAHAEQLLVAVDTAFVPFEFKQGDKYVGFDIDLWDAIARD
CCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECEEEEEEECCCCEEEEEEEHHHHHHHHH
LKLDYKLQPMDFNGILPALQTRNVDVALAGITIKEDRKKVIDFSDGYYDSGFMLMVPSSS
CCCCEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEECCCCEECCCEEEEECCCC
TIKGPNELKGKSLALKTGTSAADYAKSNFAGTDLRLFPNIDNAYLELRTGRVDAAMHDTP
CCCCCHHCCCCEEEEECCCCHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCEEECCCC
NVLYYIHTAGKGQVKAVGPQMMAQKYGIGFPKGSPLVPKVNEALAKIRADGRYAAIYKKW
CEEEEEEECCCCEEEEECHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHH
FGVEPANM
CCCCCCCC
>Mature Secondary Structure
MKLFAKFASAVCLAASLVAHAAHAEQLLVAVDTAFVPFEFKQGDKYVGFDIDLWDAIARD
CCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECEEEEEEECCCCEEEEEEEHHHHHHHHH
LKLDYKLQPMDFNGILPALQTRNVDVALAGITIKEDRKKVIDFSDGYYDSGFMLMVPSSS
CCCCEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEECCCCEECCCEEEEECCCC
TIKGPNELKGKSLALKTGTSAADYAKSNFAGTDLRLFPNIDNAYLELRTGRVDAAMHDTP
CCCCCHHCCCCEEEEECCCCHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCEEECCCC
NVLYYIHTAGKGQVKAVGPQMMAQKYGIGFPKGSPLVPKVNEALAKIRADGRYAAIYKKW
CEEEEEEECCCCEEEEECHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHH
FGVEPANM
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]