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The map label for this gene is folE [H]

Identifier: 73537506

GI number: 73537506

Start: 251526

End: 252269

Strand: Reverse

Name: folE [H]

Synonym: Reut_B3671

Alternate gene names: 73537506

Gene position: 252269-251526 (Counterclockwise)

Preceding gene: 73537507

Following gene: 73537505

Centisome position: 9.25

GC content: 63.84

Gene sequence:

>744_bases
ATGACAGACAAGGACGCTGCACACCACGGCGCGAAGCACAAGACCAGTGATGCCGCCCCCGGCGAGTCGAGCGTATCGGA
CCGCATCCGCTCGCGCCTGCTGGCGGCGCGAGAGCGCTTTCATGCCAACGACAATATCGCCCGCTACATCGAGCCGGGCG
AACTCGACGCGCTGCAGGAAGAAGTGCAGGCACGGCTGGAAGAGGTACTGCGCGCGCTCGTTATCGACGTGGACCAGGAC
CACAACACGCGCGAAACCGCGCGCCGCGTGGCCAAGATGTACCTGAAGGAAATCTTCGCGGGACGCTACGCCAAGGCGCC
GGCAGTGACAGAGTTTCCGAACGTCGAGCAACTCAGCGAACTGATGATCGTCGGGCCGCTCCGCGTGCGCAGCGCCTGTT
CGCACCACCTGTGCCCGATCATCGGCAAGCTGTGGGTGGGCGTGATGCCGAACCAGCATTCCAACCTGATCGGCCTGTCC
AAGTACGCGCGGCTGGCCGAGTGGATCATGTGCCGGCCGCAGATCCAAGAAGAAGCGGTGGCCCAGGTAGCCGACCTGCT
GCAGGAAAAGATGAACCCGGACGGCCTGGCCATCGTCATGGAAGCCGAGCACTTCTGCATGCACTGGCGCGGCGTGCGCG
ATACCGATGCCAAGATGACCAACAGCGTGATGCGCGGCTCCTTCCTGAAGGACGGCAGCCTGCGCCGCGAATTCCTCGCC
CTGCTCAATCGCAACGGCAAATAG

Upstream 100 bases:

>100_bases
GCGACGCTCGCCAGGGAGGGGCGCAGGTTGTGAGAGCCTGACATCTGCCATATAGTGAAGCACGGTGCACCATCCCTCTC
CTACCTGAAAGCGGCTCTCC

Downstream 100 bases:

>100_bases
CCATCCCGGAGCTGCCAGCATGCTCGTCCGCCTGCTCTATGCCAGCCGCGCCCGCCAGGCCATCGACGCCGCGACGCTCG
ACGCGATCCTGACCACCAGC

Product: GTP cyclohydrolase

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTDKDAAHHGAKHKTSDAAPGESSVSDRIRSRLLAARERFHANDNIARYIEPGELDALQEEVQARLEEVLRALVIDVDQD
HNTRETARRVAKMYLKEIFAGRYAKAPAVTEFPNVEQLSELMIVGPLRVRSACSHHLCPIIGKLWVGVMPNQHSNLIGLS
KYARLAEWIMCRPQIQEEAVAQVADLLQEKMNPDGLAIVMEAEHFCMHWRGVRDTDAKMTNSVMRGSFLKDGSLRREFLA
LLNRNGK

Sequences:

>Translated_247_residues
MTDKDAAHHGAKHKTSDAAPGESSVSDRIRSRLLAARERFHANDNIARYIEPGELDALQEEVQARLEEVLRALVIDVDQD
HNTRETARRVAKMYLKEIFAGRYAKAPAVTEFPNVEQLSELMIVGPLRVRSACSHHLCPIIGKLWVGVMPNQHSNLIGLS
KYARLAEWIMCRPQIQEEAVAQVADLLQEKMNPDGLAIVMEAEHFCMHWRGVRDTDAKMTNSVMRGSFLKDGSLRREFLA
LLNRNGK
>Mature_246_residues
TDKDAAHHGAKHKTSDAAPGESSVSDRIRSRLLAARERFHANDNIARYIEPGELDALQEEVQARLEEVLRALVIDVDQDH
NTRETARRVAKMYLKEIFAGRYAKAPAVTEFPNVEQLSELMIVGPLRVRSACSHHLCPIIGKLWVGVMPNQHSNLIGLSK
YARLAEWIMCRPQIQEEAVAQVADLLQEKMNPDGLAIVMEAEHFCMHWRGVRDTDAKMTNSVMRGSFLKDGSLRREFLAL
LNRNGK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=127, Percent_Identity=40.1574803149606, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI4503949, Length=127, Percent_Identity=40.1574803149606, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI66932970, Length=97, Percent_Identity=36.0824742268041, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI66932972, Length=87, Percent_Identity=39.0804597701149, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788476, Length=179, Percent_Identity=33.5195530726257, Blast_Score=110, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=129, Percent_Identity=41.0852713178295, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321706, Length=125, Percent_Identity=44, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24656796, Length=128, Percent_Identity=38.28125, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24656791, Length=128, Percent_Identity=38.28125, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24656782, Length=124, Percent_Identity=38.7096774193548, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 27875; Mature: 27744

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKDAAHHGAKHKTSDAAPGESSVSDRIRSRLLAARERFHANDNIARYIEPGELDALQE
CCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHH
EVQARLEEVLRALVIDVDQDHNTRETARRVAKMYLKEIFAGRYAKAPAVTEFPNVEQLSE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
LMIVGPLRVRSACSHHLCPIIGKLWVGVMPNQHSNLIGLSKYARLAEWIMCRPQIQEEAV
HHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
AQVADLLQEKMNPDGLAIVMEAEHFCMHWRGVRDTDAKMTNSVMRGSFLKDGSLRREFLA
HHHHHHHHHHCCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
LLNRNGK
HHHCCCC
>Mature Secondary Structure 
TDKDAAHHGAKHKTSDAAPGESSVSDRIRSRLLAARERFHANDNIARYIEPGELDALQE
CCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHH
EVQARLEEVLRALVIDVDQDHNTRETARRVAKMYLKEIFAGRYAKAPAVTEFPNVEQLSE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
LMIVGPLRVRSACSHHLCPIIGKLWVGVMPNQHSNLIGLSKYARLAEWIMCRPQIQEEAV
HHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
AQVADLLQEKMNPDGLAIVMEAEHFCMHWRGVRDTDAKMTNSVMRGSFLKDGSLRREFLA
HHHHHHHHHHCCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
LLNRNGK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA