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The map label for this gene is arsB [H]

Identifier: 73537507

GI number: 73537507

Start: 252711

End: 253970

Strand: Reverse

Name: arsB [H]

Synonym: Reut_B3672

Alternate gene names: 73537507

Gene position: 253970-252711 (Counterclockwise)

Preceding gene: 73537508

Following gene: 73537506

Centisome position: 9.32

GC content: 68.65

Gene sequence:

>1260_bases
ATGTCGAGCGTCAGCGCGGTATTGATCTGGGCAATTGCTGCCCTCACCACCGCTGGCGTGCTGCTGCGGCCGTTTCGCCT
TTCGGAGGCCTTCTGGGCGGTCGCGGGCGCGGTGCTGCTGTGCGTGGGCGGACTGCTGGCAGTGCCGGACGCGCTCGCCG
CGGTCATGCGCGGCCTGGATGTCTACCTGTTCCTGGCCGGCATGATGGTGAGTTCCGAACTGGCCCGCAAGACCGGGCTG
TTCGACCACGTCGCGGCCCTGGCCGTGCGGCGCGCAGGCGGTTCCGCCCGAGCGCTGTTCCTGCTGGTCTATGGCTTTGG
CACGCTGGTCACGGCGTTCATGTCCAACGACGCGACCGCCGTGGTGCTGACGCCTGCCGTGCTGGCCGCCACGCGCGCGG
CAAAGGTGCAGAACCCGCTGCCGCACCTGTTTGCCTGCGCCTTCATCGCCAATGCGGCCAGCTTCGTGTTGCCGATTTCC
AATCCAGCCAACCTCGTGATCTTCGGGACGCGCATGCCGCCGCTGGCCGGATGGCTCGCGCATTTTGCGCTGCCATCGCT
GGTTGCCGTGGTGACCACGTTGGCCGCGCTGTACTGGACGCAGCGCGAGGCACTGGCCGAGCGAATCGCCAGCGACGTGC
CAGTGCCGCCGCTGAGCCTGCAGGCGTGGCTGACGGCGGTTGGCATCGTGCTGACCGGGGTGGCCTTGCTGGCGGCATCG
CTGCGCGGCAGCGACCTGGGTTGGCCAACATGCGCGGCGGGCGCGGCTACGCTGCTGATCGTGTGCGCGACCAAGCGCGA
CTTGCTCGCGCCGGCCCTGCGCGAGATTTCGTGGGGCGTGTTGCCAATGGTGGCGGGGTTGTTCGTGCTGGTGGCGGCGC
TCGAGCATACCGGCGTGATCCACTGGCTTGCCGACATCATCCAGGCGGCGTCCCGCCAGGACAGCCTTGCGCGGCTTTGG
GGTATCGGGGCGGCTGTGGCGCTGATCGGCAACGTTGCCAACAATCTCCCTGCAGGGCTGATCGCGTCCTCGGCACTGGC
TGCGGGTCATGCGTCCAACGCCGTGACAGGTGCCGTGCTGGTGGGCATCGACCTGGGCCCTAACCTTTCCGCAACCGGTT
CCCTGGCCACGCTGCTGTGGCTGACCGCGCTACGCCGCGATGGTCATATGGTCACCGCCGGCCAGTTCCTGCGGCTCGGC
GCGGTTGTCATGCCGACAGCCATGGTGCCGACACTGGCCGTACTCGCGCTCGCGCGCTGA

Upstream 100 bases:

>100_bases
CCCGCAGCACCGGCCGCGAGCCGCGACGACAGGGCAAGCCTGTAGTCAAGCATCTGGGCTGCAGCGCCCTCCTTTCAACC
TCTCAACAACAAGAACCACA

Downstream 100 bases:

>100_bases
CTGTCGCGGGCGTCTGCCGCGTAGCGTCAGTCGCGGCTGGCGGACTGCGCGGCGCGCTGCGACAGCACGCCGTGCCAGCG
CAGCGGCGGCGCGCCATGAC

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSSVSAVLIWAIAALTTAGVLLRPFRLSEAFWAVAGAVLLCVGGLLAVPDALAAVMRGLDVYLFLAGMMVSSELARKTGL
FDHVAALAVRRAGGSARALFLLVYGFGTLVTAFMSNDATAVVLTPAVLAATRAAKVQNPLPHLFACAFIANAASFVLPIS
NPANLVIFGTRMPPLAGWLAHFALPSLVAVVTTLAALYWTQREALAERIASDVPVPPLSLQAWLTAVGIVLTGVALLAAS
LRGSDLGWPTCAAGAATLLIVCATKRDLLAPALREISWGVLPMVAGLFVLVAALEHTGVIHWLADIIQAASRQDSLARLW
GIGAAVALIGNVANNLPAGLIASSALAAGHASNAVTGAVLVGIDLGPNLSATGSLATLLWLTALRRDGHMVTAGQFLRLG
AVVMPTAMVPTLAVLALAR

Sequences:

>Translated_419_residues
MSSVSAVLIWAIAALTTAGVLLRPFRLSEAFWAVAGAVLLCVGGLLAVPDALAAVMRGLDVYLFLAGMMVSSELARKTGL
FDHVAALAVRRAGGSARALFLLVYGFGTLVTAFMSNDATAVVLTPAVLAATRAAKVQNPLPHLFACAFIANAASFVLPIS
NPANLVIFGTRMPPLAGWLAHFALPSLVAVVTTLAALYWTQREALAERIASDVPVPPLSLQAWLTAVGIVLTGVALLAAS
LRGSDLGWPTCAAGAATLLIVCATKRDLLAPALREISWGVLPMVAGLFVLVAALEHTGVIHWLADIIQAASRQDSLARLW
GIGAAVALIGNVANNLPAGLIASSALAAGHASNAVTGAVLVGIDLGPNLSATGSLATLLWLTALRRDGHMVTAGQFLRLG
AVVMPTAMVPTLAVLALAR
>Mature_418_residues
SSVSAVLIWAIAALTTAGVLLRPFRLSEAFWAVAGAVLLCVGGLLAVPDALAAVMRGLDVYLFLAGMMVSSELARKTGLF
DHVAALAVRRAGGSARALFLLVYGFGTLVTAFMSNDATAVVLTPAVLAATRAAKVQNPLPHLFACAFIANAASFVLPISN
PANLVIFGTRMPPLAGWLAHFALPSLVAVVTTLAALYWTQREALAERIASDVPVPPLSLQAWLTAVGIVLTGVALLAASL
RGSDLGWPTCAAGAATLLIVCATKRDLLAPALREISWGVLPMVAGLFVLVAALEHTGVIHWLADIIQAASRQDSLARLWG
IGAAVALIGNVANNLPAGLIASSALAAGHASNAVTGAVLVGIDLGPNLSATGSLATLLWLTALRRDGHMVTAGQFLRLGA
VVMPTAMVPTLAVLALAR

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=432, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 42993; Mature: 42862

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVSAVLIWAIAALTTAGVLLRPFRLSEAFWAVAGAVLLCVGGLLAVPDALAAVMRGLD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYLFLAGMMVSSELARKTGLFDHVAALAVRRAGGSARALFLLVYGFGTLVTAFMSNDATA
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
VVLTPAVLAATRAAKVQNPLPHLFACAFIANAASFVLPISNPANLVIFGTRMPPLAGWLA
EEECHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHEEEECCCCCCEEEEECCCCHHHHHHH
HFALPSLVAVVTTLAALYWTQREALAERIASDVPVPPLSLQAWLTAVGIVLTGVALLAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRGSDLGWPTCAAGAATLLIVCATKRDLLAPALREISWGVLPMVAGLFVLVAALEHTGVI
HCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
HWLADIIQAASRQDSLARLWGIGAAVALIGNVANNLPAGLIASSALAAGHASNAVTGAVL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEE
VGIDLGPNLSATGSLATLLWLTALRRDGHMVTAGQFLRLGAVVMPTAMVPTLAVLALAR
EEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSVSAVLIWAIAALTTAGVLLRPFRLSEAFWAVAGAVLLCVGGLLAVPDALAAVMRGLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYLFLAGMMVSSELARKTGLFDHVAALAVRRAGGSARALFLLVYGFGTLVTAFMSNDATA
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
VVLTPAVLAATRAAKVQNPLPHLFACAFIANAASFVLPISNPANLVIFGTRMPPLAGWLA
EEECHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHEEEECCCCCCEEEEECCCCHHHHHHH
HFALPSLVAVVTTLAALYWTQREALAERIASDVPVPPLSLQAWLTAVGIVLTGVALLAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRGSDLGWPTCAAGAATLLIVCATKRDLLAPALREISWGVLPMVAGLFVLVAALEHTGVI
HCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
HWLADIIQAASRQDSLARLWGIGAAVALIGNVANNLPAGLIASSALAAGHASNAVTGAVL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEE
VGIDLGPNLSATGSLATLLWLTALRRDGHMVTAGQFLRLGAVVMPTAMVPTLAVLALAR
EEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA