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The map label for this gene is proP [H]

Identifier: 73537462

GI number: 73537462

Start: 196774

End: 198084

Strand: Reverse

Name: proP [H]

Synonym: Reut_B3627

Alternate gene names: 73537462

Gene position: 198084-196774 (Counterclockwise)

Preceding gene: 73537465

Following gene: 73537460

Centisome position: 7.27

GC content: 65.98

Gene sequence:

>1311_bases
ATGTCCACCACCAGCAGTACCGCCCCGATCACGGGTGCGCCGGCCCAGCCCGCGCGATCTCTGACGGGCCAGGATTACAA
GACCCTTTCCCTCGCCGCACTGGGCGGCGCCCTCGAGTTCTACGACTTCATCATCTTCGTGTTCTTCGCGACGGTGATCG
GCCAGCTGTTCTTCCCGCCGTCCGTACCAGACTGGCTGCGCCAGCTCCAGACCTTCGGCATCTTTGCCGCGGGCTACCTG
GCTCGCCCGCTCGGCGGCATCATCATGGCTCACTTCGGCGACCTGCTGGGCCGCAAGAAGATGTTCACGCTGTCGATCCT
GCTGATGTCGGTCCCCACGCTGCTGATGGGCCTGATGCCGACCTACCAGACCATCGGCCTGCTGGCTCCCATGCTGCTAC
TGGTGCTGCGTATCCTGCAAGGGGCCGCCGTAGGTGGCGAAGTGCCGGGCGCCTGGGTGTTCGTCTCGGAGCACGTGCCG
GAGCGCCACGTCGGCTATGCGTGCGGCACGCTGACCTGCGGCCTGACGGCCGGGATCCTGCTCGGCTCGCTCGTGGCTAC
CGGCATCAATTCGGTCTTTACGCCTGCCGAACTCGCGGACTGGGGTTGGCGCGTGCCGTTCCTGATCGGCGGCGTGTTCG
GCATTACGTCGATGTACCTGCGCCGCTGGCTGCACGAAACGCCGGTCTTCGCCGAATTGCAGAAGCGCAAGGCTCTGGCG
GCGGAAATGCCGCTGAAGTCAGTGGTGCGCGACCATCGCGGCGCGGTCGTCGTGTCGATGCTGCTGACGTGGATGCTGTC
GGCGGGCATTGTCGTGGTGATCCTGATGACCCCGACCTTCCTGCAGAAGCTGTACGGCTTTGACGCGCGCACGGCGCTGA
TCGCCAACAGCGTGGCAACGCTGTGCCTGACCTTCGGCTGCGTGATATCAGGAGCGCTGGCCGACCGCATCGGCGCGCGC
CGCACGCTGTTCTTCGGCGGCCTGCTGCTGGCCGCCAGCGCCTACGCGCTCTACACCACCGTCCAAACCCGGCCCGACCT
GCTGGTACCGCTGTATGCGCTCGCCGGCTTCCTGGTCGGCACCATCGGCGCCGTGCCGTTCGTGCTGGTCAACGCGTTCC
CTGCCCAGGTGCGTTTTTCGGGCCTGTCGTTCTCGTACAACCTCTCGTATGCGATCTTCGGCGGCCTGACTCCGATGGTC
GTCACGTTGATGCTCAAGAACGATCCGCTGGCGCCGGCGCACTATGTGGTCGCCATCTGCGCGCTCGGCATGGTGACGGC
GTTGTTCGTCAAGGATCGCCCGCGCGGCTGA

Upstream 100 bases:

>100_bases
ACGTAGTGCCTGCTTCTCCCGCGCTGTACCCTCCGGCCACACCCTCCACGCAATCCGTGTTGTGCGCTTCGCATGAGATG
CCATTCATGCGAGTCAACTC

Downstream 100 bases:

>100_bases
TGCGCAGCGGCGAGTTGCCAGGCACTGCGGCAAGTTGCCACAACATCAAGGAGGGCTGCCTGCCCTCCTTTTTCATGGTC
GCCGGCTACCGGCTGTGTTC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSTTSSTAPITGAPAQPARSLTGQDYKTLSLAALGGALEFYDFIIFVFFATVIGQLFFPPSVPDWLRQLQTFGIFAAGYL
ARPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLMPTYQTIGLLAPMLLLVLRILQGAAVGGEVPGAWVFVSEHVP
ERHVGYACGTLTCGLTAGILLGSLVATGINSVFTPAELADWGWRVPFLIGGVFGITSMYLRRWLHETPVFAELQKRKALA
AEMPLKSVVRDHRGAVVVSMLLTWMLSAGIVVVILMTPTFLQKLYGFDARTALIANSVATLCLTFGCVISGALADRIGAR
RTLFFGGLLLAASAYALYTTVQTRPDLLVPLYALAGFLVGTIGAVPFVLVNAFPAQVRFSGLSFSYNLSYAIFGGLTPMV
VTLMLKNDPLAPAHYVVAICALGMVTALFVKDRPRG

Sequences:

>Translated_436_residues
MSTTSSTAPITGAPAQPARSLTGQDYKTLSLAALGGALEFYDFIIFVFFATVIGQLFFPPSVPDWLRQLQTFGIFAAGYL
ARPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLMPTYQTIGLLAPMLLLVLRILQGAAVGGEVPGAWVFVSEHVP
ERHVGYACGTLTCGLTAGILLGSLVATGINSVFTPAELADWGWRVPFLIGGVFGITSMYLRRWLHETPVFAELQKRKALA
AEMPLKSVVRDHRGAVVVSMLLTWMLSAGIVVVILMTPTFLQKLYGFDARTALIANSVATLCLTFGCVISGALADRIGAR
RTLFFGGLLLAASAYALYTTVQTRPDLLVPLYALAGFLVGTIGAVPFVLVNAFPAQVRFSGLSFSYNLSYAIFGGLTPMV
VTLMLKNDPLAPAHYVVAICALGMVTALFVKDRPRG
>Mature_435_residues
STTSSTAPITGAPAQPARSLTGQDYKTLSLAALGGALEFYDFIIFVFFATVIGQLFFPPSVPDWLRQLQTFGIFAAGYLA
RPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLMPTYQTIGLLAPMLLLVLRILQGAAVGGEVPGAWVFVSEHVPE
RHVGYACGTLTCGLTAGILLGSLVATGINSVFTPAELADWGWRVPFLIGGVFGITSMYLRRWLHETPVFAELQKRKALAA
EMPLKSVVRDHRGAVVVSMLLTWMLSAGIVVVILMTPTFLQKLYGFDARTALIANSVATLCLTFGCVISGALADRIGARR
TLFFGGLLLAASAYALYTTVQTRPDLLVPLYALAGFLVGTIGAVPFVLVNAFPAQVRFSGLSFSYNLSYAIFGGLTPMVV
TLMLKNDPLAPAHYVVAICALGMVTALFVKDRPRG

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=422, Percent_Identity=29.8578199052133, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788292, Length=449, Percent_Identity=29.8440979955457, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788942, Length=414, Percent_Identity=32.1256038647343, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789941, Length=424, Percent_Identity=29.4811320754717, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082231, Length=484, Percent_Identity=23.3471074380165, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46681; Mature: 46550

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STTSSTAPITGAPAQPARSLTGQDYKTLSLAALGGALEFYDFIIFVFFATVIGQLFFPP
CCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SVPDWLRQLQTFGIFAAGYLARPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLMP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TYQTIGLLAPMLLLVLRILQGAAVGGEVPGAWVFVSEHVPERHVGYACGTLTCGLTAGIL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHCCHHHHHHHHHHHHHHH
LGSLVATGINSVFTPAELADWGWRVPFLIGGVFGITSMYLRRWLHETPVFAELQKRKALA
HHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AEMPLKSVVRDHRGAVVVSMLLTWMLSAGIVVVILMTPTFLQKLYGFDARTALIANSVAT
HHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHEEHHHCHHHHHHHHCCCHHHHHHHHHHHH
LCLTFGCVISGALADRIGARRTLFFGGLLLAASAYALYTTVQTRPDLLVPLYALAGFLVG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TIGAVPFVLVNAFPAQVRFSGLSFSYNLSYAIFGGLTPMVVTLMLKNDPLAPAHYVVAIC
HHHHHHHHHHHCCCHHEEECCCEEEEECHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHH
ALGMVTALFVKDRPRG
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]