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The map label for this gene is pbuE [H]

Identifier: 73543104

GI number: 73543104

Start: 3756486

End: 3757658

Strand: Direct

Name: pbuE [H]

Synonym: Reut_A3422

Alternate gene names: 73543104

Gene position: 3756486-3757658 (Clockwise)

Preceding gene: 73543103

Following gene: 73543105

Centisome position: 98.69

GC content: 68.63

Gene sequence:

>1173_bases
ATGGACCCCAGGCTAGTGACGCTCTGCGTCGGCAATTTCATCATTGGCACCGGCGCGATGATCGTTACCGGCATGTTGAA
CGACATTGCCGCCGATTTCGGCCTGGGCGTGGCCAGCGCCGGCCAGTTGATCTCGGTGTTCGCGCTGGCCACCTGTGTGG
GGGCGCCGCTGTTCGCCACGCTGGGCTCGCGTATCGACCGCCGGCTGTTGCTGGCCGGTTCGCTGCTGATCTATGCGGTG
ATGCATCTGGCCGCGGCGCTGGCGCCGAGCTTCGCGACGCTGATGGCGATCCGCTTCCTGACGGCGATCGGCGCCGCCAT
CTATACGCCGCAGACCGCGGCCACCCTGCCCTTGCTGGTCAACGCGCAGACGCGCGGCCGCGCCATCAGCTTCGTGTTCC
TCGGCTGGAGTATCGCCAGCGTGGTCGGCATGCCGCTTGGCACCTGGATTGCCAGCACACTCGGCTGGCGCTTCAGCATG
GCGGTCGTCGGCGTGCTGGCGCTGCTGGTGTCGGCGGCGGTGTGGCGTGCGTTGCCGCGCGGGCTGTACGTGCCGGCCGT
GGGGCGTGAAGCCTGGGACGCCGTGCTGCGCCACCGGCCGATCATGCTCGTGGTGGCCGCCACGATGATCCAGTCGGCCG
GCATGTTCACGATGTTTACCTACATTGCGCCGCTGATGCGCGATGCCTACGGCATCACCGGTGCCGCGCTGAGCCTGATG
TTCCTGGCCTATGGCGCCTGCGGGGTACTCGGCAACGCGGTGGCGGCGTCGCGCATGGACCGGATCACGCCGAGCCGGAT
TGTCCAGTTCACGCTGCTGACGTCGACCGCGGCGATGGTGCTGTGGCCGCTCGCGGGCCTGGGCAGCGTCGCGCTGGTGC
TGCTGTTCATGCTGTGGGGCATCGGCGGGTTTGCCACCAACAGCGCACAGCAGGCGCGGCTGGTCATGATCGAGCCCGAC
CGTGCATCGGTGTCGATCTCGCTCAACTCATCGTCGATCTATCTCGGCCAGGCGCTGGGCGCCATGGCTGGTGCGGCCAT
CTATACGGTGGCGGGCACCGGTTCGCTGCACTGGGGTGCAGCGGCGCTGACGCTGGTGGCGCTGGCGGTTTCGCAGTGGG
CGCGCGCGCTGGGCCGCCAGTGGCCGCGTCGGCAAGCGCCACAACAGGCCTGA

Upstream 100 bases:

>100_bases
GCTACACTCGGGACTTCCCTGACCGCAGGCCGCCCATGGCCCGCGGCGCATCCCCGACAACCACAAGGCGCCTGCGCGCC
GCCCCTCCAGCAAGGCCGGA

Downstream 100 bases:

>100_bases
ACTGGGCCGGGCCGTTGCCCGCAATCCTCTGAGCGGCGTCTCGCTCCACAGCTTTTCCACAGTGTTGTTCAACGCACTGT
CCACCACCTGTTCCCTGCGT

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MDPRLVTLCVGNFIIGTGAMIVTGMLNDIAADFGLGVASAGQLISVFALATCVGAPLFATLGSRIDRRLLLAGSLLIYAV
MHLAAALAPSFATLMAIRFLTAIGAAIYTPQTAATLPLLVNAQTRGRAISFVFLGWSIASVVGMPLGTWIASTLGWRFSM
AVVGVLALLVSAAVWRALPRGLYVPAVGREAWDAVLRHRPIMLVVAATMIQSAGMFTMFTYIAPLMRDAYGITGAALSLM
FLAYGACGVLGNAVAASRMDRITPSRIVQFTLLTSTAAMVLWPLAGLGSVALVLLFMLWGIGGFATNSAQQARLVMIEPD
RASVSISLNSSSIYLGQALGAMAGAAIYTVAGTGSLHWGAAALTLVALAVSQWARALGRQWPRRQAPQQA

Sequences:

>Translated_390_residues
MDPRLVTLCVGNFIIGTGAMIVTGMLNDIAADFGLGVASAGQLISVFALATCVGAPLFATLGSRIDRRLLLAGSLLIYAV
MHLAAALAPSFATLMAIRFLTAIGAAIYTPQTAATLPLLVNAQTRGRAISFVFLGWSIASVVGMPLGTWIASTLGWRFSM
AVVGVLALLVSAAVWRALPRGLYVPAVGREAWDAVLRHRPIMLVVAATMIQSAGMFTMFTYIAPLMRDAYGITGAALSLM
FLAYGACGVLGNAVAASRMDRITPSRIVQFTLLTSTAAMVLWPLAGLGSVALVLLFMLWGIGGFATNSAQQARLVMIEPD
RASVSISLNSSSIYLGQALGAMAGAAIYTVAGTGSLHWGAAALTLVALAVSQWARALGRQWPRRQAPQQA
>Mature_390_residues
MDPRLVTLCVGNFIIGTGAMIVTGMLNDIAADFGLGVASAGQLISVFALATCVGAPLFATLGSRIDRRLLLAGSLLIYAV
MHLAAALAPSFATLMAIRFLTAIGAAIYTPQTAATLPLLVNAQTRGRAISFVFLGWSIASVVGMPLGTWIASTLGWRFSM
AVVGVLALLVSAAVWRALPRGLYVPAVGREAWDAVLRHRPIMLVVAATMIQSAGMFTMFTYIAPLMRDAYGITGAALSLM
FLAYGACGVLGNAVAASRMDRITPSRIVQFTLLTSTAAMVLWPLAGLGSVALVLLFMLWGIGGFATNSAQQARLVMIEPD
RASVSISLNSSSIYLGQALGAMAGAAIYTVAGTGSLHWGAAALTLVALAVSQWARALGRQWPRRQAPQQA

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=327, Percent_Identity=27.5229357798165, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786595, Length=395, Percent_Identity=25.3164556962025, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787808, Length=389, Percent_Identity=24.4215938303342, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI145693198, Length=276, Percent_Identity=24.2753623188406, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24654044, Length=163, Percent_Identity=26.9938650306748, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40747; Mature: 40747

Theoretical pI: Translated: 11.33; Mature: 11.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPRLVTLCVGNFIIGTGAMIVTGMLNDIAADFGLGVASAGQLISVFALATCVGAPLFAT
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHH
LGSRIDRRLLLAGSLLIYAVMHLAAALAPSFATLMAIRFLTAIGAAIYTPQTAATLPLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEE
NAQTRGRAISFVFLGWSIASVVGMPLGTWIASTLGWRFSMAVVGVLALLVSAAVWRALPR
ECCCCCCEEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLYVPAVGREAWDAVLRHRPIMLVVAATMIQSAGMFTMFTYIAPLMRDAYGITGAALSLM
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHH
FLAYGACGVLGNAVAASRMDRITPSRIVQFTLLTSTAAMVLWPLAGLGSVALVLLFMLWG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
IGGFATNSAQQARLVMIEPDRASVSISLNSSSIYLGQALGAMAGAAIYTVAGTGSLHWGA
CCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEECCCCCCHHH
AALTLVALAVSQWARALGRQWPRRQAPQQA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDPRLVTLCVGNFIIGTGAMIVTGMLNDIAADFGLGVASAGQLISVFALATCVGAPLFAT
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHH
LGSRIDRRLLLAGSLLIYAVMHLAAALAPSFATLMAIRFLTAIGAAIYTPQTAATLPLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEE
NAQTRGRAISFVFLGWSIASVVGMPLGTWIASTLGWRFSMAVVGVLALLVSAAVWRALPR
ECCCCCCEEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLYVPAVGREAWDAVLRHRPIMLVVAATMIQSAGMFTMFTYIAPLMRDAYGITGAALSLM
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHH
FLAYGACGVLGNAVAASRMDRITPSRIVQFTLLTSTAAMVLWPLAGLGSVALVLLFMLWG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
IGGFATNSAQQARLVMIEPDRASVSISLNSSSIYLGQALGAMAGAAIYTVAGTGSLHWGA
CCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEECCCCCCHHH
AALTLVALAVSQWARALGRQWPRRQAPQQA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]