Click here to switch to the map view.

The map label for this gene is livM [H]

Identifier: 73543049

GI number: 73543049

Start: 3686439

End: 3687467

Strand: Reverse

Name: livM [H]

Synonym: Reut_A3367

Alternate gene names: 73543049

Gene position: 3687467-3686439 (Counterclockwise)

Preceding gene: 73543050

Following gene: 73543048

Centisome position: 96.87

GC content: 64.53

Gene sequence:

>1029_bases
ATGCGTGCAGAATCCACTGTGACGATCAAGTCCGGACAGGCAAAAGAGGGCACGGGCGTGCAAAAGAAACTGTTGTACGG
ACTGCTGCTGCTCGCGCTGGTGGCAGCGCCGATGGCCGGCGCCTATCCGGTGTTCGTGCTCAAGGTGTTGTGCTTTGCGC
TGTTCGCGTGCGCGTTCAACCTGCTGATCGGCTATACCGGGCTGCTGTCGTTCGGGCACGCGGCGTTCTTCGGCGGCGCG
GGCTATATCTCGGGCGAAGCGATGAAGGTCTGGGGCGTGACGCCCGAGATCGGCCTGATCTTGGGTACTGCCGGCGGCGC
GGCGATTGGCTACGTGGTCGGCTCGCTGGCCATCCGCCGCCAGGGCATCTACTTCTCGATGATCACGCTGGCGCTGGCGC
AGATGCTGTTCTTCATCTGCCTGCAGGCGCCGTTCACGGGCGGTGAGGACGGCCTGCAGGGCATCCCGCGCGGCAAGCTG
TTCGGCGTGCTGTCGCTGTCGGACGACCTGACGCTGTACTACGTGGCGCTGGTGATCATCGTGGCGGCGTTCGCGCTCAT
TGTGCGCACCGTGCATTCGCCGTTCGGCCAGATTCTCAAGGCGATCAAGGAGAACGAGCCGCGCGCGATTTCGCTCGGCT
ACGATGTGGACCGCTTCAAGCTGACCGCCTTCGTGCTGTCTGCCGCGCTGTCCGGCCTGGCCGGTTCGATCAAGGCGCTG
GTGCTCGGCTTCGAGACGCTGACCGACGTGCACTGGTCGATGTCGGGTTCCGTGATCCTGATGACGCTGGTCGGCGGCCT
CGGCACATTGTCGGGCCCGATTGTCGGCGCCTTCGTGGTGGTGGCGCTGGAGAACAAGCTCGGCGATATCGGTGCGTTCC
TGGCTTCGGCCACTGGCTTCCAGTGGTTCAACTCGCTGGGCGAGTCGGTGACCATGGTGATCGGCGCGATCTTCGTGATC
TGCGTGCTGACCTTCCGCCGCGGCATCATGGGCGAGCTGATCGCGCTCTTCGGCCGCAAGCAGGAGTGA

Upstream 100 bases:

>100_bases
CCAAGGTGTTCTATCCTGAAGCATCGTCGACCGTCGTGTTCTTCATCATGGTGATCGTGCTGCTGCTGCGTCCGGCCGGA
CTGTTCGGGAGGGAGAAGTG

Downstream 100 bases:

>100_bases
TCACCGGTCGACATGCCCGGAATTGGGGCTAGGGTTTCTTCTAGCTTGTCCCATTTGCACCGGTTCGTTACATTTCACAC
ACGGTTTTCGCAGGTTAAAT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRAESTVTIKSGQAKEGTGVQKKLLYGLLLLALVAAPMAGAYPVFVLKVLCFALFACAFNLLIGYTGLLSFGHAAFFGGA
GYISGEAMKVWGVTPEIGLILGTAGGAAIGYVVGSLAIRRQGIYFSMITLALAQMLFFICLQAPFTGGEDGLQGIPRGKL
FGVLSLSDDLTLYYVALVIIVAAFALIVRTVHSPFGQILKAIKENEPRAISLGYDVDRFKLTAFVLSAALSGLAGSIKAL
VLGFETLTDVHWSMSGSVILMTLVGGLGTLSGPIVGAFVVVALENKLGDIGAFLASATGFQWFNSLGESVTMVIGAIFVI
CVLTFRRGIMGELIALFGRKQE

Sequences:

>Translated_342_residues
MRAESTVTIKSGQAKEGTGVQKKLLYGLLLLALVAAPMAGAYPVFVLKVLCFALFACAFNLLIGYTGLLSFGHAAFFGGA
GYISGEAMKVWGVTPEIGLILGTAGGAAIGYVVGSLAIRRQGIYFSMITLALAQMLFFICLQAPFTGGEDGLQGIPRGKL
FGVLSLSDDLTLYYVALVIIVAAFALIVRTVHSPFGQILKAIKENEPRAISLGYDVDRFKLTAFVLSAALSGLAGSIKAL
VLGFETLTDVHWSMSGSVILMTLVGGLGTLSGPIVGAFVVVALENKLGDIGAFLASATGFQWFNSLGESVTMVIGAIFVI
CVLTFRRGIMGELIALFGRKQE
>Mature_342_residues
MRAESTVTIKSGQAKEGTGVQKKLLYGLLLLALVAAPMAGAYPVFVLKVLCFALFACAFNLLIGYTGLLSFGHAAFFGGA
GYISGEAMKVWGVTPEIGLILGTAGGAAIGYVVGSLAIRRQGIYFSMITLALAQMLFFICLQAPFTGGEDGLQGIPRGKL
FGVLSLSDDLTLYYVALVIIVAAFALIVRTVHSPFGQILKAIKENEPRAISLGYDVDRFKLTAFVLSAALSGLAGSIKAL
VLGFETLTDVHWSMSGSVILMTLVGGLGTLSGPIVGAFVVVALENKLGDIGAFLASATGFQWFNSLGESVTMVIGAIFVI
CVLTFRRGIMGELIALFGRKQE

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=272, Percent_Identity=25, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35914; Mature: 35914

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAESTVTIKSGQAKEGTGVQKKLLYGLLLLALVAAPMAGAYPVFVLKVLCFALFACAFN
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LLIGYTGLLSFGHAAFFGGAGYISGEAMKVWGVTPEIGLILGTAGGAAIGYVVGSLAIRR
HHHHHHHHHHHHHHHHHCCCCEECCCCEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHH
QGIYFSMITLALAQMLFFICLQAPFTGGEDGLQGIPRGKLFGVLSLSDDLTLYYVALVII
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHHH
VAAFALIVRTVHSPFGQILKAIKENEPRAISLGYDVDRFKLTAFVLSAALSGLAGSIKAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLGFETLTDVHWSMSGSVILMTLVGGLGTLSGPIVGAFVVVALENKLGDIGAFLASATGF
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QWFNSLGESVTMVIGAIFVICVLTFRRGIMGELIALFGRKQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRAESTVTIKSGQAKEGTGVQKKLLYGLLLLALVAAPMAGAYPVFVLKVLCFALFACAFN
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LLIGYTGLLSFGHAAFFGGAGYISGEAMKVWGVTPEIGLILGTAGGAAIGYVVGSLAIRR
HHHHHHHHHHHHHHHHHCCCCEECCCCEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHH
QGIYFSMITLALAQMLFFICLQAPFTGGEDGLQGIPRGKLFGVLSLSDDLTLYYVALVII
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHHHH
VAAFALIVRTVHSPFGQILKAIKENEPRAISLGYDVDRFKLTAFVLSAALSGLAGSIKAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLGFETLTDVHWSMSGSVILMTLVGGLGTLSGPIVGAFVVVALENKLGDIGAFLASATGF
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QWFNSLGESVTMVIGAIFVICVLTFRRGIMGELIALFGRKQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]