Click here to switch to the map view.

The map label for this gene is braD [H]

Identifier: 73543050

GI number: 73543050

Start: 3687467

End: 3688357

Strand: Reverse

Name: braD [H]

Synonym: Reut_A3368

Alternate gene names: 73543050

Gene position: 3688357-3687467 (Counterclockwise)

Preceding gene: 73543051

Following gene: 73543049

Centisome position: 96.9

GC content: 62.4

Gene sequence:

>891_bases
ATGCCTATGGACATCTTCGGAATTCCTCTTCCCGCCATGCTGTCCCAGCTGCTGCTGGGCCTCGTCAATGGCGCCTTCTA
TGCGATGCTGAGCCTGGGCCTGGCGGTGATCTTCGGCCTGCTCAACGTCATCAATTTCGCGCACGGCGCGCTGTTCATGC
TCGGGGCCGTGCTGGCCTGGATGGGCATGGAGTACGCGGGGCTCAACTACTGGGTCATGCTGATCCTGGCACCGCTCGCT
GTGGCGGTGATAGGCGTGGTCATCGAAAAGACCATGCTGCGCTGGATCTACAAGCTCGACCATATCTACGGCCTGCTGCT
CACGCTCGGCATCACGCTGGTGATCGAAGGGATCTTCCGCTCGATCTACGGCGTATCGGGCCTGCCGTACCAGACGCCGG
ATGCGCTGCAGGGCGCGACCGACCTTGGCTTCATGATCCTGCCGAACTATCGCGCGTGGGTCGTGGTGGCGTCGCTGGTG
GTGTGCTTTGCCACATGGTACGTGATCGAGAAGACCAAGCTCGGCGCCTACCTGCGCGCCGGTACCGAGAACCCGAAGAT
GGTGGAAGCCTTCGGCGTCAACGTGCCGCTGATGGTGACGCTGACCTACGGCTTCGGCGTGGCGCTGGCGGCGTTTGCGG
GGGTGCTCGCGGCGCCGGTGATCCAGATTTCGCCGCTGATGGGGCAGAACCTGATCATCATCGTGTTCGCGGTGGTAGTG
ATCGGCGGCATGGGGTCGATCATGGGTTCGATCCTGACCGGCCTGGGCCTCGGCGTGATCGAAGGGCTGACCAAGGTGTT
CTATCCTGAAGCATCGTCGACCGTCGTGTTCTTCATCATGGTGATCGTGCTGCTGCTGCGTCCGGCCGGACTGTTCGGGA
GGGAGAAGTGA

Upstream 100 bases:

>100_bases
TAAGGAAGCAGGCAGGCTCGGGCAATCCGCGAGCCCGCCGGTTTGCTCCCCTTTCCCGCTTCTTCCGCTAGCGGGAGAGG
GGAGATACCACCAGCAACGG

Downstream 100 bases:

>100_bases
TGCGTGCAGAATCCACTGTGACGATCAAGTCCGGACAGGCAAAAGAGGGCACGGGCGTGCAAAAGAAACTGTTGTACGGA
CTGCTGCTGCTCGCGCTGGT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MPMDIFGIPLPAMLSQLLLGLVNGAFYAMLSLGLAVIFGLLNVINFAHGALFMLGAVLAWMGMEYAGLNYWVMLILAPLA
VAVIGVVIEKTMLRWIYKLDHIYGLLLTLGITLVIEGIFRSIYGVSGLPYQTPDALQGATDLGFMILPNYRAWVVVASLV
VCFATWYVIEKTKLGAYLRAGTENPKMVEAFGVNVPLMVTLTYGFGVALAAFAGVLAAPVIQISPLMGQNLIIIVFAVVV
IGGMGSIMGSILTGLGLGVIEGLTKVFYPEASSTVVFFIMVIVLLLRPAGLFGREK

Sequences:

>Translated_296_residues
MPMDIFGIPLPAMLSQLLLGLVNGAFYAMLSLGLAVIFGLLNVINFAHGALFMLGAVLAWMGMEYAGLNYWVMLILAPLA
VAVIGVVIEKTMLRWIYKLDHIYGLLLTLGITLVIEGIFRSIYGVSGLPYQTPDALQGATDLGFMILPNYRAWVVVASLV
VCFATWYVIEKTKLGAYLRAGTENPKMVEAFGVNVPLMVTLTYGFGVALAAFAGVLAAPVIQISPLMGQNLIIIVFAVVV
IGGMGSIMGSILTGLGLGVIEGLTKVFYPEASSTVVFFIMVIVLLLRPAGLFGREK
>Mature_295_residues
PMDIFGIPLPAMLSQLLLGLVNGAFYAMLSLGLAVIFGLLNVINFAHGALFMLGAVLAWMGMEYAGLNYWVMLILAPLAV
AVIGVVIEKTMLRWIYKLDHIYGLLLTLGITLVIEGIFRSIYGVSGLPYQTPDALQGATDLGFMILPNYRAWVVVASLVV
CFATWYVIEKTKLGAYLRAGTENPKMVEAFGVNVPLMVTLTYGFGVALAAFAGVLAAPVIQISPLMGQNLIIIVFAVVVI
GGMGSIMGSILTGLGLGVIEGLTKVFYPEASSTVVFFIMVIVLLLRPAGLFGREK

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=307, Percent_Identity=25.7328990228013, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31716; Mature: 31585

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMDIFGIPLPAMLSQLLLGLVNGAFYAMLSLGLAVIFGLLNVINFAHGALFMLGAVLAW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGMEYAGLNYWVMLILAPLAVAVIGVVIEKTMLRWIYKLDHIYGLLLTLGITLVIEGIFR
HCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIYGVSGLPYQTPDALQGATDLGFMILPNYRAWVVVASLVVCFATWYVIEKTKLGAYLRA
HHHCCCCCCCCCCHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTENPKMVEAFGVNVPLMVTLTYGFGVALAAFAGVLAAPVIQISPLMGQNLIIIVFAVVV
CCCCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHH
IGGMGSIMGSILTGLGLGVIEGLTKVFYPEASSTVVFFIMVIVLLLRPAGLFGREK
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PMDIFGIPLPAMLSQLLLGLVNGAFYAMLSLGLAVIFGLLNVINFAHGALFMLGAVLAW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGMEYAGLNYWVMLILAPLAVAVIGVVIEKTMLRWIYKLDHIYGLLLTLGITLVIEGIFR
HCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIYGVSGLPYQTPDALQGATDLGFMILPNYRAWVVVASLVVCFATWYVIEKTKLGAYLRA
HHHCCCCCCCCCCHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTENPKMVEAFGVNVPLMVTLTYGFGVALAAFAGVLAAPVIQISPLMGQNLIIIVFAVVV
CCCCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHH
IGGMGSIMGSILTGLGLGVIEGLTKVFYPEASSTVVFFIMVIVLLLRPAGLFGREK
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]