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The map label for this gene is sdaA [H]

Identifier: 73543015

GI number: 73543015

Start: 3644110

End: 3645486

Strand: Direct

Name: sdaA [H]

Synonym: Reut_A3333

Alternate gene names: 73543015

Gene position: 3644110-3645486 (Clockwise)

Preceding gene: 73543014

Following gene: 73543016

Centisome position: 95.73

GC content: 67.61

Gene sequence:

>1377_bases
GTGGCAGTCAGCGTCTTCGATCTGTTCAAGGTGGGCATCGGCCCGTCGAGCTCACACACCGTGGGCCCGATGCGCGCGGC
CCTGATGTTTGCCCAGGGCCTGGAGCGCGACGCCCTGCTGCCGCAGGTCGCCAGTGTGCGCGTGGAGTTGTACGGTTCGC
TCGGCGCGACCGGCAAGGGCCACGGCACCGACAAGGGCGTGATGCTGGGCCTGATGGGCGAAGCGCCCGATACCATCGAT
CCCGATTCCGTCGACGGCCGGTTGCATGCGTTGCGCGCGAGCCGCACGCTGTCGCTGCTCGGCCGGCATCCGATCCCGTT
CGTCGAGAAGGAGCACATTGCCTTCTACCGGCGCGAGGCCATGGCCGAACACCCGAACGGCATGAAGTTCCACGCGTATG
ACGCGCAGGGGACGTCGCTGCGCGAGGCACGCTATCTGTCGGTCGGCGGCGGCTTCGTCGTGACCGCGGGCGCGCCGAAC
ACGCAGGTACTCAACGCGGCGCAGCAACTGCCGCATCCGTTCCGCAGCGGCAAGGACATGCTGGAGATGGCGAACGAAAG
CGGCAAGTCCATCGCGCGGCTGATGATGGAGAACGAACTCGTCTGGCGCAGCGAGGAAGACGTCACCGCCGGGCTGCTGC
ATATCTGGGACGTGATGCAGGCCTGCGTCGCGCGTGGCTGCCGCACCGATGGCGAACTGCCGGGCCCGTTCAAGGTCCGA
CGTCGCGCGCCCGAGCTGTACCGCAACCTGACGGAGCGCGCCGAGCGCACGCTGTCCGATCCGCTGTCCGTGATGGACTG
GGTCAACCTTTACGCCATGGCCGTCAACGAAGAGAACGCCGCGGGCGGCCGCGTAGTGACGGCGCCGACCAACGGCGCAG
CCGGCATCATCCCGGCCGTGCTCCATTATTACGACCGCTTCGTGCCCGGCGCCGACCGCAAGGGCGTGGTCGATTTCCTG
CTGACCGCCGGCGCCATCGGCCTGCTCTACAAGCTCAATGCGTCGATCTCGGGGGCCGAGGTCGGCTGCCAGGGCGAAGT
CGGCGTGGCGTGCTCGATGGCCGCCGGCGCGCTCGCGGCTGTGCTCGGCGGCACGCCTGAGCAGGTCGAGAACGCTGCCG
AGATCGGCATGGAGCACAACCTGGGCCTGACCTGCGATCCGGTCGGCGGACTGGTACAGATTCCGTGCATCGAACGCAAT
GCCATGGCCTCGGTCAAGGCCGTGAACGCAGCGCGCATGGCGCTGCGCGGCGACGGCACGCACTACGTGTCGCTCGATTC
GGTCATCAAGACCATGCGCGAGACCGGCGCCGACATGAAGACCAAGTACAAGGAAACGGCGCGTGGCGGCCTTGCGGTGA
ATATCGTGGAGTGCTGA

Upstream 100 bases:

>100_bases
CGCCGGGGACCTCGATCTGGAACCAGTCGAACAAGGACGAGGCGGTCTGACCTTTCGTCACCCTTCCCACATCACCTGCA
GTCGCTTTCAGGAGACTTTC

Downstream 100 bases:

>100_bases
GGCCTTAGCGGCGTATCATGGCGCCAGTCTCCCCGGGCGCCACCTCAGGCGCCTGCAGAAACAGAGCAAAAAGCGAGGCG
CCATGCAAACCTTCTATCAG

Product: L-serine ammonia-lyase

Products: NA

Alternate protein names: SDH; L-serine deaminase; L-SD [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MAVSVFDLFKVGIGPSSSHTVGPMRAALMFAQGLERDALLPQVASVRVELYGSLGATGKGHGTDKGVMLGLMGEAPDTID
PDSVDGRLHALRASRTLSLLGRHPIPFVEKEHIAFYRREAMAEHPNGMKFHAYDAQGTSLREARYLSVGGGFVVTAGAPN
TQVLNAAQQLPHPFRSGKDMLEMANESGKSIARLMMENELVWRSEEDVTAGLLHIWDVMQACVARGCRTDGELPGPFKVR
RRAPELYRNLTERAERTLSDPLSVMDWVNLYAMAVNEENAAGGRVVTAPTNGAAGIIPAVLHYYDRFVPGADRKGVVDFL
LTAGAIGLLYKLNASISGAEVGCQGEVGVACSMAAGALAAVLGGTPEQVENAAEIGMEHNLGLTCDPVGGLVQIPCIERN
AMASVKAVNAARMALRGDGTHYVSLDSVIKTMRETGADMKTKYKETARGGLAVNIVEC

Sequences:

>Translated_458_residues
MAVSVFDLFKVGIGPSSSHTVGPMRAALMFAQGLERDALLPQVASVRVELYGSLGATGKGHGTDKGVMLGLMGEAPDTID
PDSVDGRLHALRASRTLSLLGRHPIPFVEKEHIAFYRREAMAEHPNGMKFHAYDAQGTSLREARYLSVGGGFVVTAGAPN
TQVLNAAQQLPHPFRSGKDMLEMANESGKSIARLMMENELVWRSEEDVTAGLLHIWDVMQACVARGCRTDGELPGPFKVR
RRAPELYRNLTERAERTLSDPLSVMDWVNLYAMAVNEENAAGGRVVTAPTNGAAGIIPAVLHYYDRFVPGADRKGVVDFL
LTAGAIGLLYKLNASISGAEVGCQGEVGVACSMAAGALAAVLGGTPEQVENAAEIGMEHNLGLTCDPVGGLVQIPCIERN
AMASVKAVNAARMALRGDGTHYVSLDSVIKTMRETGADMKTKYKETARGGLAVNIVEC
>Mature_457_residues
AVSVFDLFKVGIGPSSSHTVGPMRAALMFAQGLERDALLPQVASVRVELYGSLGATGKGHGTDKGVMLGLMGEAPDTIDP
DSVDGRLHALRASRTLSLLGRHPIPFVEKEHIAFYRREAMAEHPNGMKFHAYDAQGTSLREARYLSVGGGFVVTAGAPNT
QVLNAAQQLPHPFRSGKDMLEMANESGKSIARLMMENELVWRSEEDVTAGLLHIWDVMQACVARGCRTDGELPGPFKVRR
RAPELYRNLTERAERTLSDPLSVMDWVNLYAMAVNEENAAGGRVVTAPTNGAAGIIPAVLHYYDRFVPGADRKGVVDFLL
TAGAIGLLYKLNASISGAEVGCQGEVGVACSMAAGALAAVLGGTPEQVENAAEIGMEHNLGLTCDPVGGLVQIPCIERNA
MASVKAVNAARMALRGDGTHYVSLDSVIKTMRETGADMKTKYKETARGGLAVNIVEC

Specific function: Deaminates Also Threonine, Particularly When It Is Present In High Concentration. [C]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI1788116, Length=457, Percent_Identity=54.0481400437637, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1789161, Length=457, Percent_Identity=52.9540481400438, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI48994925, Length=455, Percent_Identity=52.7472527472528, Blast_Score=459, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 48787; Mature: 48655

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSVFDLFKVGIGPSSSHTVGPMRAALMFAQGLERDALLPQVASVRVELYGSLGATGKG
CEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCCC
HGTDKGVMLGLMGEAPDTIDPDSVDGRLHALRASRTLSLLGRHPIPFVEKEHIAFYRREA
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MAEHPNGMKFHAYDAQGTSLREARYLSVGGGFVVTAGAPNTQVLNAAQQLPHPFRSGKDM
HHCCCCCCEEEEECCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHCCCCHHCCHHH
LEMANESGKSIARLMMENELVWRSEEDVTAGLLHIWDVMQACVARGCRTDGELPGPFKVR
HHHHHCCHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
RRAPELYRNLTERAERTLSDPLSVMDWVNLYAMAVNEENAAGGRVVTAPTNGAAGIIPAV
HCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHHHHHH
LHYYDRFVPGADRKGVVDFLLTAGAIGLLYKLNASISGAEVGCQGEVGVACSMAAGALAA
HHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
VLGGTPEQVENAAEIGMEHNLGLTCDPVGGLVQIPCIERNAMASVKAVNAARMALRGDGT
HHCCCHHHHHHHHHCCCCCCCCEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHEECCCC
HYVSLDSVIKTMRETGADMKTKYKETARGGLAVNIVEC
EEEEHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
AVSVFDLFKVGIGPSSSHTVGPMRAALMFAQGLERDALLPQVASVRVELYGSLGATGKG
EEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCCC
HGTDKGVMLGLMGEAPDTIDPDSVDGRLHALRASRTLSLLGRHPIPFVEKEHIAFYRREA
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
MAEHPNGMKFHAYDAQGTSLREARYLSVGGGFVVTAGAPNTQVLNAAQQLPHPFRSGKDM
HHCCCCCCEEEEECCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHCCCCHHCCHHH
LEMANESGKSIARLMMENELVWRSEEDVTAGLLHIWDVMQACVARGCRTDGELPGPFKVR
HHHHHCCHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
RRAPELYRNLTERAERTLSDPLSVMDWVNLYAMAVNEENAAGGRVVTAPTNGAAGIIPAV
HCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHHHHHH
LHYYDRFVPGADRKGVVDFLLTAGAIGLLYKLNASISGAEVGCQGEVGVACSMAAGALAA
HHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
VLGGTPEQVENAAEIGMEHNLGLTCDPVGGLVQIPCIERNAMASVKAVNAARMALRGDGT
HHCCCHHHHHHHHHCCCCCCCCEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHEECCCC
HYVSLDSVIKTMRETGADMKTKYKETARGGLAVNIVEC
EEEEHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]