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The map label for this gene is alkK [H]

Identifier: 73542966

GI number: 73542966

Start: 3590397

End: 3592058

Strand: Direct

Name: alkK [H]

Synonym: Reut_A3283

Alternate gene names: 73542966

Gene position: 3590397-3592058 (Clockwise)

Preceding gene: 73542965

Following gene: 73542967

Centisome position: 94.32

GC content: 64.32

Gene sequence:

>1662_bases
ATGCTGGGCCTCATGCAAGACCGTCCGTTGTTGATTTCATCGCTGATCGAGTACGCAGCGGCGTACCACCCGCAACAGGA
AATCATCTCGCGCACCGTGGAAGGCGGCACGGTGCGCACCAATTACGACCAGGTGCACCGCCGCGCGAAGCGGCTCGCCA
ACGCGCTCGCGAAGCTGGGTCTCAGCGAAGGCGACCGCATCGGCACGCTCGCGTGGAATACGCACCGGCACCTGGAGATG
TACTTCGGCGTATCGGGCTCGGGCGCCGTGCTGCATACAGTCAACCCGCGGCTCTTTCCCGAGCAGATCGAATACATCAT
CAACCACGCCGAAGACAGCGTGCTCTGCTTCGACAGCTGCTTCGCACCACTGGTGGCCAAGCTGGCGCCGCAGTTGAAGA
CCGTGCGGCACTACGTGGCGATGACCGATCCCGAACACATGGCCGCGCTGGCCAGTGCCGGCATTCCCAATCTGGTCTGC
TACGAAGACCTGCTTGCCGACAGCAGCGAGGACTACGAATGGCCGCAGTTCGACGAGCGCACCGCGTCGTCGCTGTGCTA
CACGTCAGGGACCACGGGCAATCCCAAAGGCGTGCTGTATTCGCACCGCTCCACCGTGCTGCATAGCCTCAAGGCGAGCG
GCTCCGACACGTTCGGCGTGAGCCCTGAAAGCAGCATCCTGATGATCGTGCCGCTGTTCCACGCGAACGCCTGGGGCCTG
CCCTATGCGTGCGCGATGAACGGCGCAAAGATGGTGCTGCCTGCCCAGCATCTCGACGGCGAAAGCGTGTACCGCTTGCT
GCGCGACGAGCGCGTGAACTACTCGACCGCCGTGCCCACGGTGTGGCTGATGCTGTTCCAGTATCTCGACGCGCATCCGG
AAATCGATCCGCGCGAGCTGAGCCTGCGCATCGCCGGCGTGGGCGGATCGGCCGCGCCCGCGGCGATGATCGAGCGCTTC
GAAAAGCAGTTCGGCGCGACGCTGATGCAAGGCTGGGGCATGACCGAGACCAGCCCCATCGGCGTGATCAACACGCTGCT
GCCGCAGCACGCGGGCCTTAGCGAAGCCGAGCAGCTCAAGATCAAGCTCAAGCAAGGCCGCGCGCTGTGGGGCGTGGAAC
TGCGTATCGAGGACGATGAAGGCCGGGTGCTGCCGCACGACGGCCAGGCGTTCGGCAAGCTCAAGGTACGCGGGCCGTGG
ATTGCGTCGTCCTACTTCAAGGCCGAACACGATGCGCTCGACAATGACGGCTGGTTCGATACCGGCGACGTGGCCAACAT
CGATCCCGAAGGCTATGTGCAGCTCGTCGACCGCGCCAAGGACGTGATCAAGTCGGGCGGCGAGTGGATTTCGTCGATCG
ACCTCGAGAACGCGACCATGAGCCATCCCGCCGTGGCCGAGGCAGCTGTCATTGGCGTGCCGCATCCCAAGTGGCAGGAG
CGGCCCCTGCTGATTGTTGTGAAACGCCCGGACCACGAAGTCACGTCCGCCGAATTGATCGCCTTCCTGGCCGGCAAGGT
CGTGCGCTGGTGGGTGCCCGACGATGTGGTGTTCGTCGATGCATTGCCGCACACCGCCACCGGCAAGCTGCTCAAGGTGA
AGCTGCGCGACGCGTACCGCAACTACGTCCTGCCGACCGTGCGGGACGAACGCATCGCATAG

Upstream 100 bases:

>100_bases
CCGCCGCAGTTCAAGGGCCGCTGATCGCCCCGTCGCCGTTTGCAGCACCCCCGGGATGGCCGTCCCGGGGCATCACGAGA
ACAACACAGGGAGACGCATC

Downstream 100 bases:

>100_bases
ACCGTGATGCTCGCGCTCCCCGGTGCGGGCATTCATCCCGAACACTGTACGAATCAATAAGGAGCCGCGCATGAGCGCCA
GCAGCACCGACCGCATCATT

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MLGLMQDRPLLISSLIEYAAAYHPQQEIISRTVEGGTVRTNYDQVHRRAKRLANALAKLGLSEGDRIGTLAWNTHRHLEM
YFGVSGSGAVLHTVNPRLFPEQIEYIINHAEDSVLCFDSCFAPLVAKLAPQLKTVRHYVAMTDPEHMAALASAGIPNLVC
YEDLLADSSEDYEWPQFDERTASSLCYTSGTTGNPKGVLYSHRSTVLHSLKASGSDTFGVSPESSILMIVPLFHANAWGL
PYACAMNGAKMVLPAQHLDGESVYRLLRDERVNYSTAVPTVWLMLFQYLDAHPEIDPRELSLRIAGVGGSAAPAAMIERF
EKQFGATLMQGWGMTETSPIGVINTLLPQHAGLSEAEQLKIKLKQGRALWGVELRIEDDEGRVLPHDGQAFGKLKVRGPW
IASSYFKAEHDALDNDGWFDTGDVANIDPEGYVQLVDRAKDVIKSGGEWISSIDLENATMSHPAVAEAAVIGVPHPKWQE
RPLLIVVKRPDHEVTSAELIAFLAGKVVRWWVPDDVVFVDALPHTATGKLLKVKLRDAYRNYVLPTVRDERIA

Sequences:

>Translated_553_residues
MLGLMQDRPLLISSLIEYAAAYHPQQEIISRTVEGGTVRTNYDQVHRRAKRLANALAKLGLSEGDRIGTLAWNTHRHLEM
YFGVSGSGAVLHTVNPRLFPEQIEYIINHAEDSVLCFDSCFAPLVAKLAPQLKTVRHYVAMTDPEHMAALASAGIPNLVC
YEDLLADSSEDYEWPQFDERTASSLCYTSGTTGNPKGVLYSHRSTVLHSLKASGSDTFGVSPESSILMIVPLFHANAWGL
PYACAMNGAKMVLPAQHLDGESVYRLLRDERVNYSTAVPTVWLMLFQYLDAHPEIDPRELSLRIAGVGGSAAPAAMIERF
EKQFGATLMQGWGMTETSPIGVINTLLPQHAGLSEAEQLKIKLKQGRALWGVELRIEDDEGRVLPHDGQAFGKLKVRGPW
IASSYFKAEHDALDNDGWFDTGDVANIDPEGYVQLVDRAKDVIKSGGEWISSIDLENATMSHPAVAEAAVIGVPHPKWQE
RPLLIVVKRPDHEVTSAELIAFLAGKVVRWWVPDDVVFVDALPHTATGKLLKVKLRDAYRNYVLPTVRDERIA
>Mature_553_residues
MLGLMQDRPLLISSLIEYAAAYHPQQEIISRTVEGGTVRTNYDQVHRRAKRLANALAKLGLSEGDRIGTLAWNTHRHLEM
YFGVSGSGAVLHTVNPRLFPEQIEYIINHAEDSVLCFDSCFAPLVAKLAPQLKTVRHYVAMTDPEHMAALASAGIPNLVC
YEDLLADSSEDYEWPQFDERTASSLCYTSGTTGNPKGVLYSHRSTVLHSLKASGSDTFGVSPESSILMIVPLFHANAWGL
PYACAMNGAKMVLPAQHLDGESVYRLLRDERVNYSTAVPTVWLMLFQYLDAHPEIDPRELSLRIAGVGGSAAPAAMIERF
EKQFGATLMQGWGMTETSPIGVINTLLPQHAGLSEAEQLKIKLKQGRALWGVELRIEDDEGRVLPHDGQAFGKLKVRGPW
IASSYFKAEHDALDNDGWFDTGDVANIDPEGYVQLVDRAKDVIKSGGEWISSIDLENATMSHPAVAEAAVIGVPHPKWQE
RPLLIVVKRPDHEVTSAELIAFLAGKVVRWWVPDDVVFVDALPHTATGKLLKVKLRDAYRNYVLPTVRDERIA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=555, Percent_Identity=25.5855855855856, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI187761345, Length=537, Percent_Identity=26.4432029795158, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI187761343, Length=537, Percent_Identity=26.4432029795158, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI38505220, Length=517, Percent_Identity=24.7582205029014, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI28416953, Length=559, Percent_Identity=25.4025044722719, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI115511026, Length=520, Percent_Identity=22.5, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI157311624, Length=522, Percent_Identity=23.9463601532567, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157311622, Length=522, Percent_Identity=23.9463601532567, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI58082049, Length=525, Percent_Identity=24.1904761904762, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI42544132, Length=520, Percent_Identity=23.2692307692308, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI122937307, Length=529, Percent_Identity=24.007561436673, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI13375727, Length=546, Percent_Identity=25.0915750915751, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI8923896, Length=401, Percent_Identity=24.4389027431421, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI253970489, Length=401, Percent_Identity=24.4389027431421, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788107, Length=566, Percent_Identity=26.5017667844523, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI145693145, Length=513, Percent_Identity=26.9005847953216, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI221142682, Length=529, Percent_Identity=23.4404536862004, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1786810, Length=519, Percent_Identity=26.2042389210019, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1790505, Length=536, Percent_Identity=25.5597014925373, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1789201, Length=364, Percent_Identity=25, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=517, Percent_Identity=27.852998065764, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71994703, Length=573, Percent_Identity=26.0034904013962, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71994694, Length=573, Percent_Identity=26.0034904013962, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71994690, Length=573, Percent_Identity=26.0034904013962, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32563687, Length=520, Percent_Identity=26.1538461538462, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17557194, Length=531, Percent_Identity=25.8003766478343, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=517, Percent_Identity=25.1450676982592, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=525, Percent_Identity=23.8095238095238, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17559526, Length=381, Percent_Identity=28.6089238845144, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=536, Percent_Identity=24.8134328358209, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17560140, Length=511, Percent_Identity=23.679060665362, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=380, Percent_Identity=27.8947368421053, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=505, Percent_Identity=22.5742574257426, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=377, Percent_Identity=28.9124668435013, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320852, Length=153, Percent_Identity=32.0261437908497, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323182, Length=389, Percent_Identity=23.1362467866324, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319264, Length=384, Percent_Identity=23.6979166666667, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581924, Length=569, Percent_Identity=27.5922671353251, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI18859661, Length=505, Percent_Identity=26.5346534653465, Blast_Score=137, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21355181, Length=516, Percent_Identity=26.7441860465116, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24648260, Length=517, Percent_Identity=25.3384912959381, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19922652, Length=510, Percent_Identity=23.921568627451, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356947, Length=512, Percent_Identity=24.0234375, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653035, Length=517, Percent_Identity=23.5976789168279, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24656500, Length=510, Percent_Identity=23.5294117647059, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648255, Length=535, Percent_Identity=22.4299065420561, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45550496, Length=574, Percent_Identity=22.4738675958188, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45552801, Length=574, Percent_Identity=22.4738675958188, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648253, Length=535, Percent_Identity=22.2429906542056, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI161076582, Length=525, Percent_Identity=24.1904761904762, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21355225, Length=563, Percent_Identity=24.1563055062167, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130357, Length=533, Percent_Identity=21.9512195121951, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356441, Length=504, Percent_Identity=21.6269841269841, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI62472339, Length=390, Percent_Identity=25.6410256410256, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24667959, Length=395, Percent_Identity=25.3164556962025, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24667955, Length=378, Percent_Identity=25.6613756613757, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI281365686, Length=258, Percent_Identity=20.1550387596899, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 61117; Mature: 61117

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLMQDRPLLISSLIEYAAAYHPQQEIISRTVEGGTVRTNYDQVHRRAKRLANALAKLG
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCC
LSEGDRIGTLAWNTHRHLEMYFGVSGSGAVLHTVNPRLFPEQIEYIINHAEDSVLCFDSC
CCCCCCEEEEEECCCCEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEHHHH
FAPLVAKLAPQLKTVRHYVAMTDPEHMAALASAGIPNLVCYEDLLADSSEDYEWPQFDER
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHH
TASSLCYTSGTTGNPKGVLYSHRSTVLHSLKASGSDTFGVSPESSILMIVPLFHANAWGL
HHHHEEEECCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCC
PYACAMNGAKMVLPAQHLDGESVYRLLRDERVNYSTAVPTVWLMLFQYLDAHPEIDPREL
CHHHHCCCCEEEEEHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
SLRIAGVGGSAAPAAMIERFEKQFGATLMQGWGMTETSPIGVINTLLPQHAGLSEAEQLK
EEEEEECCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCHHHHH
IKLKQGRALWGVELRIEDDEGRVLPHDGQAFGKLKVRGPWIASSYFKAEHDALDNDGWFD
EEEECCCEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCEEEHHHHHHHHCCCCCCCCCC
TGDVANIDPEGYVQLVDRAKDVIKSGGEWISSIDLENATMSHPAVAEAAVIGVPHPKWQE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCCCCCHHHEEEEECCCCCCCC
RPLLIVVKRPDHEVTSAELIAFLAGKVVRWWVPDDVVFVDALPHTATGKLLKVKLRDAYR
CCEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCEEEEEEHHHHH
NYVLPTVRDERIA
HCCCCCCCCCCCC
>Mature Secondary Structure
MLGLMQDRPLLISSLIEYAAAYHPQQEIISRTVEGGTVRTNYDQVHRRAKRLANALAKLG
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCC
LSEGDRIGTLAWNTHRHLEMYFGVSGSGAVLHTVNPRLFPEQIEYIINHAEDSVLCFDSC
CCCCCCEEEEEECCCCEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEHHHH
FAPLVAKLAPQLKTVRHYVAMTDPEHMAALASAGIPNLVCYEDLLADSSEDYEWPQFDER
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHH
TASSLCYTSGTTGNPKGVLYSHRSTVLHSLKASGSDTFGVSPESSILMIVPLFHANAWGL
HHHHEEEECCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCC
PYACAMNGAKMVLPAQHLDGESVYRLLRDERVNYSTAVPTVWLMLFQYLDAHPEIDPREL
CHHHHCCCCEEEEEHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
SLRIAGVGGSAAPAAMIERFEKQFGATLMQGWGMTETSPIGVINTLLPQHAGLSEAEQLK
EEEEEECCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCHHHHH
IKLKQGRALWGVELRIEDDEGRVLPHDGQAFGKLKVRGPWIASSYFKAEHDALDNDGWFD
EEEECCCEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCEEEHHHHHHHHCCCCCCCCCC
TGDVANIDPEGYVQLVDRAKDVIKSGGEWISSIDLENATMSHPAVAEAAVIGVPHPKWQE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCCCCCHHHEEEEECCCCCCCC
RPLLIVVKRPDHEVTSAELIAFLAGKVVRWWVPDDVVFVDALPHTATGKLLKVKLRDAYR
CCEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCEEEEEEHHHHH
NYVLPTVRDERIA
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]