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The map label for this gene is dctA2 [H]

Identifier: 73542962

GI number: 73542962

Start: 3586083

End: 3587474

Strand: Direct

Name: dctA2 [H]

Synonym: Reut_A3279

Alternate gene names: 73542962

Gene position: 3586083-3587474 (Clockwise)

Preceding gene: 73542959

Following gene: 73542963

Centisome position: 94.21

GC content: 65.23

Gene sequence:

>1392_bases
ATGCAGCACGCAGTCCAGTCGCGCCCCGCCCCTGGTGGCCGCCTCCACACCCGCTTCACCCGGTCGCTGTTCGGCCAGGT
GCTGATCGCCCTGGTGATCGGCACGGCGCTCGGCATGCTGTTCCCCGAATTCGCCGCCAAGCTCAAGCCGCTCGGCGATG
CGTTTATCAAGTTGGTCAAGATGCTGATCGGCCCCATCGTGTTCTGCGTGGTCGTGGCCGGCATCTGCGGCGCGGGCGAG
CTCAAGAAGGTCGGCCGTGTCGGCATCAAGGCGGTGGTGTACTTCGAGATCGTCACGACCATCGCGCTGGCGCTCGGCAT
CCTGCTGGCCTATGTCTTTCACCCGGGCACGGGCATGAACGTGGACCCCCGCTCGCTCGATGCGTCGGCCATCTCCGGCT
ATATCGAGAACGCCACCAAGGTGAAGAGCGAGGGCACCGCCGACTTCCTGCTCAAGCTGATCCCGAACACGATCATCGGC
GCCTTCTCCAGCGGCGACGTGCTGCAGGTGCTGCTGGTCTCGGTGCTGTTCGGTTGTGCGCTGTCGCTGGTCGGAGAAAA
GGCCAAGCCGCTCGTGAGCCTGATCGACACGTTCTCGCACACGCTGTTCAAGATGATGGGCTTCATCATCAAGCTGGCCC
CGCTGGGCGTGCTGGGCGCTGTGGCCTTCACCGTCGGCAAGTACGGCATTGGCTCGCTCAAGCAGCTCGGCTTCCTGGTG
GTGCTGTTCTACGGCGCGGTGACCATCTTCGTGCTGGTGGTGCTGGGCACCATCATGCGGCTGTGCGGCTTCTCTGTGAT
CAAGCTGATCCGCTACCTGCGCGCCGAACTGCTCGTGGTGCTGGGCACTGCTTCGTCGGACAGCGTTTTGCCGCAGGTGA
TGAAGAAGCTCGAGTTCATGGGCATCAAGAAATCCGTGGTCGGCCTCGTAATCCCGACTGGCTATTCTTTCAACCTTGAC
GCGTTCTCGATCTACCTGACGCTGGCCGCCGTGTTCATCGCGCAAGCTACCAACACGCCGCTGGCGATGGGCGACCTGCT
CGGCATCCTGGCGGTGGCGCTGGTCACCTCCAAGGGGGCCCACGGCATCCCGGGCTCGGCCATCGTGATTCTGGCGGCGA
CGCTGTCGGCGCACCCGGCTATCCCGGCTATCGGCCTGGTGCTGGTGCTGTCGGTGGACTGGTTCATCGGCATTGCGCGT
GCGCTCGGCAACCTGATCGGCAACTGCGTCGCCACCGTCGTCGTGGCGGCTTGGGAGAAGGACATCGACCGTGACCGCGC
GCGGGACGTGCTCAACGGTGTGGTGCCGGCCACCGAGCTGGACGAAGGCCTGCCGATGGCCGACGCGGCCCACAATGCAG
TGCCGGTGCCGTCCCCCGCGGCGGGGCGCTAG

Upstream 100 bases:

>100_bases
GGCAGCCCATTGGCCAACGAAGCACCGGGCTGCAGGCGTTCCTGAATCAAAACGGGCAGCCCGGCGACAGGCCAGGCAAC
GACCCGGTACGGAGACAACC

Downstream 100 bases:

>100_bases
AATCTCGGCATTTCCCCAAACGCGCAGGCCCTGCGCCCAAGCAAGCCATGCCCGAGCATATCGATCCCGACATGACCGCC
GAAGCGATTGCCGATGACAT

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MQHAVQSRPAPGGRLHTRFTRSLFGQVLIALVIGTALGMLFPEFAAKLKPLGDAFIKLVKMLIGPIVFCVVVAGICGAGE
LKKVGRVGIKAVVYFEIVTTIALALGILLAYVFHPGTGMNVDPRSLDASAISGYIENATKVKSEGTADFLLKLIPNTIIG
AFSSGDVLQVLLVSVLFGCALSLVGEKAKPLVSLIDTFSHTLFKMMGFIIKLAPLGVLGAVAFTVGKYGIGSLKQLGFLV
VLFYGAVTIFVLVVLGTIMRLCGFSVIKLIRYLRAELLVVLGTASSDSVLPQVMKKLEFMGIKKSVVGLVIPTGYSFNLD
AFSIYLTLAAVFIAQATNTPLAMGDLLGILAVALVTSKGAHGIPGSAIVILAATLSAHPAIPAIGLVLVLSVDWFIGIAR
ALGNLIGNCVATVVVAAWEKDIDRDRARDVLNGVVPATELDEGLPMADAAHNAVPVPSPAAGR

Sequences:

>Translated_463_residues
MQHAVQSRPAPGGRLHTRFTRSLFGQVLIALVIGTALGMLFPEFAAKLKPLGDAFIKLVKMLIGPIVFCVVVAGICGAGE
LKKVGRVGIKAVVYFEIVTTIALALGILLAYVFHPGTGMNVDPRSLDASAISGYIENATKVKSEGTADFLLKLIPNTIIG
AFSSGDVLQVLLVSVLFGCALSLVGEKAKPLVSLIDTFSHTLFKMMGFIIKLAPLGVLGAVAFTVGKYGIGSLKQLGFLV
VLFYGAVTIFVLVVLGTIMRLCGFSVIKLIRYLRAELLVVLGTASSDSVLPQVMKKLEFMGIKKSVVGLVIPTGYSFNLD
AFSIYLTLAAVFIAQATNTPLAMGDLLGILAVALVTSKGAHGIPGSAIVILAATLSAHPAIPAIGLVLVLSVDWFIGIAR
ALGNLIGNCVATVVVAAWEKDIDRDRARDVLNGVVPATELDEGLPMADAAHNAVPVPSPAAGR
>Mature_463_residues
MQHAVQSRPAPGGRLHTRFTRSLFGQVLIALVIGTALGMLFPEFAAKLKPLGDAFIKLVKMLIGPIVFCVVVAGICGAGE
LKKVGRVGIKAVVYFEIVTTIALALGILLAYVFHPGTGMNVDPRSLDASAISGYIENATKVKSEGTADFLLKLIPNTIIG
AFSSGDVLQVLLVSVLFGCALSLVGEKAKPLVSLIDTFSHTLFKMMGFIIKLAPLGVLGAVAFTVGKYGIGSLKQLGFLV
VLFYGAVTIFVLVVLGTIMRLCGFSVIKLIRYLRAELLVVLGTASSDSVLPQVMKKLEFMGIKKSVVGLVIPTGYSFNLD
AFSIYLTLAAVFIAQATNTPLAMGDLLGILAVALVTSKGAHGIPGSAIVILAATLSAHPAIPAIGLVLVLSVDWFIGIAR
ALGNLIGNCVATVVVAAWEKDIDRDRARDVLNGVVPATELDEGLPMADAAHNAVPVPSPAAGR

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=491, Percent_Identity=24.0325865580448, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194239697, Length=501, Percent_Identity=25.5489021956088, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI40254478, Length=473, Percent_Identity=25.5813953488372, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI66773030, Length=489, Percent_Identity=25.9713701431493, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI21314632, Length=459, Percent_Identity=25.0544662309368, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI4827012, Length=329, Percent_Identity=26.7477203647416, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI5032093, Length=427, Percent_Identity=25.7611241217799, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI223468564, Length=299, Percent_Identity=27.0903010033445, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI223468566, Length=299, Percent_Identity=27.0903010033445, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI262359914, Length=381, Percent_Identity=24.1469816272966, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI301601644, Length=160, Percent_Identity=31.875, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1789947, Length=428, Percent_Identity=51.6355140186916, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1790514, Length=425, Percent_Identity=41.1764705882353, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1788024, Length=400, Percent_Identity=22.5, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=385, Percent_Identity=25.7142857142857, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=486, Percent_Identity=21.8106995884774, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71983099, Length=468, Percent_Identity=24.7863247863248, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71983106, Length=468, Percent_Identity=24.7863247863248, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71996953, Length=419, Percent_Identity=25.5369928400955, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=418, Percent_Identity=23.444976076555, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17541374, Length=402, Percent_Identity=23.6318407960199, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24583025, Length=420, Percent_Identity=25.4761904761905, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137668, Length=420, Percent_Identity=25.4761904761905, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24583023, Length=420, Percent_Identity=25.4761904761905, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281360481, Length=425, Percent_Identity=24.4705882352941, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281360483, Length=425, Percent_Identity=24.4705882352941, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17137666, Length=425, Percent_Identity=24.4705882352941, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 48411; Mature: 48411

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]