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The map label for this gene is amaB [H]

Identifier: 73542961

GI number: 73542961

Start: 3584426

End: 3585679

Strand: Reverse

Name: amaB [H]

Synonym: Reut_A3278

Alternate gene names: 73542961

Gene position: 3585679-3584426 (Counterclockwise)

Preceding gene: 73542968

Following gene: 73542960

Centisome position: 94.2

GC content: 68.02

Gene sequence:

>1254_bases
ATGGCAGCAAACCTGTCGCCGTCCGCAGACATCGGCACGCGCATCATGACCTGGGCCGACGCCCTGGCCGTTCACACCGA
GCAGCCCGGCTTGCTCACCCGCACCTATCTGACCGAGGCGCACCACGGCGCGGCCGCACAGCTGACCGAATGGATGCAGG
CCGCCGGCATGACCGTGCGCCGTGACGCCGCCGGCAACGTGATCGGCCGCTATGAAGGCACAGACCCGGGCGCCCCGGCG
CTGCTGACAGGTTCGCATTTCGACACCGTGCGCGATGCCGGGCGCTTTGACGGCAACCTCGGCGTGATCCTGCCGGTCGC
ATGCGTGGCGGAATGGAATCGCCAGGGCAAGCGCTTCCCGTTCGCGATCGAGGTGGTCGGCTTCGCCGAAGAAGAGGGCG
TACGTTTCAAGGCGACGCTGCTCGGTAGCCGCGCCATTGCCGGCACCTTCGACAACAACGTCCTCGACAACGTCGACGAC
AGCGGCAAGACCATGCGCGAAGTGATGGCCGCGGCCGGTTTCGACCCGGCCGGCCTGCCCGCCGCCGCGCACGACCCGAA
GAAAGTGCTGGCCTTTATCGAAGTCCATATCGAACAGGGCCCTGTACTGCTCAATGAAGGCCTGCCAGTCGGCGTGGTGA
CCGCGATTTCGGGTGCGACCCGCTTCGTCATCGAACTCGAAGGCCTCGCCGGCCACGCCGGCACGGTGCCGATGGACATG
CGCCGCGATGCGGCCATGGCGGGTGCCGAAATCGGCCTTTACATCGAAAAGCGCTGCGGCGGCAAGCCGGGCCTGGTGGG
CACCGTGGGCCAGTTCAACGTGCCGAACGGCGCGACCAACACGGTGCCGGGCCGCGCGGTGTTCTCGATCGACATCCGCG
CCGGCGAGGACGCCGAGCGCGAAGCCGCCGTGAACGACGTACTGGCCGAGATCGAACGCGTGTGCGCACGCCGCAACGTG
CGTGCGCAGGTGCGCAAGACGCACGAAGCCAAGAGTGTTCCCTGCGCCTCGTGGCTGCAGGAGCAATGGGCCGCCGCCGT
CGCCCGCCAGGGCGTGCCGGTGCGCCACCTTCCCTCCGGCGCCGGCCATGACTCCATGGCCATTGCCGCGATTGCCGACG
TCGCGATGCTGTTCGTGCGCTGCGGCAACGGCGGCATCAGCCACCACCCGACCGAAACCATGACCGCCGAGGACGCCGAA
CTGTCCGCGCGCGTGTTCAGCGATTTCGTCGAGCACTTCCAGCGGCAGCAATAA

Upstream 100 bases:

>100_bases
AGCTTCGGCCCATGGGGCGCCTGTACCAGGCATAGCCCCGACTGGCACCTCGCCAGCCAGGCGTGCCGCGGCTTCAAGCC
CTTCCAGCAGGAGACAACGC

Downstream 100 bases:

>100_bases
GGCTGTCCCCCGAACGCTTCCTGTTACTAAGATTCCCAGAAAACAAGCCATGACCGCACGAGACAATATGAACCCGATCG
AAACCACGCTGACGACGTTC

Product: allantoate amidohydrolase

Products: NA

Alternate protein names: L-carbamoylase [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MAANLSPSADIGTRIMTWADALAVHTEQPGLLTRTYLTEAHHGAAAQLTEWMQAAGMTVRRDAAGNVIGRYEGTDPGAPA
LLTGSHFDTVRDAGRFDGNLGVILPVACVAEWNRQGKRFPFAIEVVGFAEEEGVRFKATLLGSRAIAGTFDNNVLDNVDD
SGKTMREVMAAAGFDPAGLPAAAHDPKKVLAFIEVHIEQGPVLLNEGLPVGVVTAISGATRFVIELEGLAGHAGTVPMDM
RRDAAMAGAEIGLYIEKRCGGKPGLVGTVGQFNVPNGATNTVPGRAVFSIDIRAGEDAEREAAVNDVLAEIERVCARRNV
RAQVRKTHEAKSVPCASWLQEQWAAAVARQGVPVRHLPSGAGHDSMAIAAIADVAMLFVRCGNGGISHHPTETMTAEDAE
LSARVFSDFVEHFQRQQ

Sequences:

>Translated_417_residues
MAANLSPSADIGTRIMTWADALAVHTEQPGLLTRTYLTEAHHGAAAQLTEWMQAAGMTVRRDAAGNVIGRYEGTDPGAPA
LLTGSHFDTVRDAGRFDGNLGVILPVACVAEWNRQGKRFPFAIEVVGFAEEEGVRFKATLLGSRAIAGTFDNNVLDNVDD
SGKTMREVMAAAGFDPAGLPAAAHDPKKVLAFIEVHIEQGPVLLNEGLPVGVVTAISGATRFVIELEGLAGHAGTVPMDM
RRDAAMAGAEIGLYIEKRCGGKPGLVGTVGQFNVPNGATNTVPGRAVFSIDIRAGEDAEREAAVNDVLAEIERVCARRNV
RAQVRKTHEAKSVPCASWLQEQWAAAVARQGVPVRHLPSGAGHDSMAIAAIADVAMLFVRCGNGGISHHPTETMTAEDAE
LSARVFSDFVEHFQRQQ
>Mature_416_residues
AANLSPSADIGTRIMTWADALAVHTEQPGLLTRTYLTEAHHGAAAQLTEWMQAAGMTVRRDAAGNVIGRYEGTDPGAPAL
LTGSHFDTVRDAGRFDGNLGVILPVACVAEWNRQGKRFPFAIEVVGFAEEEGVRFKATLLGSRAIAGTFDNNVLDNVDDS
GKTMREVMAAAGFDPAGLPAAAHDPKKVLAFIEVHIEQGPVLLNEGLPVGVVTAISGATRFVIELEGLAGHAGTVPMDMR
RDAAMAGAEIGLYIEKRCGGKPGLVGTVGQFNVPNGATNTVPGRAVFSIDIRAGEDAEREAAVNDVLAEIERVCARRNVR
AQVRKTHEAKSVPCASWLQEQWAAAVARQGVPVRHLPSGAGHDSMAIAAIADVAMLFVRCGNGGISHHPTETMTAEDAEL
SARVFSDFVEHFQRQQ

Specific function: Involved In The Anaerobic Utilization Of Allantoin. Converts Allantoate To (S)-Ureidoglycolate And Ammonia. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI1786726, Length=375, Percent_Identity=33.0666666666667, Blast_Score=177, Evalue=9e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010158
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.87 [H]

Molecular weight: Translated: 44212; Mature: 44080

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANLSPSADIGTRIMTWADALAVHTEQPGLLTRTYLTEAHHGAAAQLTEWMQAAGMTVR
CCCCCCCCCCHHHHHHHHHHHHHEECCCCCCEEHHHHHHCCCCHHHHHHHHHHHCCCEEE
RDAAGNVIGRYEGTDPGAPALLTGSHFDTVRDAGRFDGNLGVILPVACVAEWNRQGKRFP
ECCCCCEEEECCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
FAIEVVGFAEEEGVRFKATLLGSRAIAGTFDNNVLDNVDDSGKTMREVMAAAGFDPAGLP
EEEEEEEECCCCCCEEEEEEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
AAAHDPKKVLAFIEVHIEQGPVLLNEGLPVGVVTAISGATRFVIELEGLAGHAGTVPMDM
CCCCCHHHHEEEHEEEECCCCEEEECCCCEEHHHHHCCCEEEEEEEECCCCCCCCCCHHH
RRDAAMAGAEIGLYIEKRCGGKPGLVGTVGQFNVPNGATNTVPGRAVFSIDIRAGEDAER
HHHHHHCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHH
EAAVNDVLAEIERVCARRNVRAQVRKTHEAKSVPCASWLQEQWAAAVARQGVPVRHLPSG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEECCCC
AGHDSMAIAAIADVAMLFVRCGNGGISHHPTETMTAEDAELSARVFSDFVEHFQRQQ
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AANLSPSADIGTRIMTWADALAVHTEQPGLLTRTYLTEAHHGAAAQLTEWMQAAGMTVR
CCCCCCCCCHHHHHHHHHHHHHEECCCCCCEEHHHHHHCCCCHHHHHHHHHHHCCCEEE
RDAAGNVIGRYEGTDPGAPALLTGSHFDTVRDAGRFDGNLGVILPVACVAEWNRQGKRFP
ECCCCCEEEECCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
FAIEVVGFAEEEGVRFKATLLGSRAIAGTFDNNVLDNVDDSGKTMREVMAAAGFDPAGLP
EEEEEEEECCCCCCEEEEEEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
AAAHDPKKVLAFIEVHIEQGPVLLNEGLPVGVVTAISGATRFVIELEGLAGHAGTVPMDM
CCCCCHHHHEEEHEEEECCCCEEEECCCCEEHHHHHCCCEEEEEEEECCCCCCCCCCHHH
RRDAAMAGAEIGLYIEKRCGGKPGLVGTVGQFNVPNGATNTVPGRAVFSIDIRAGEDAER
HHHHHHCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHH
EAAVNDVLAEIERVCARRNVRAQVRKTHEAKSVPCASWLQEQWAAAVARQGVPVRHLPSG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEECCCC
AGHDSMAIAAIADVAMLFVRCGNGGISHHPTETMTAEDAELSARVFSDFVEHFQRQQ
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9023955; 8285691 [H]