Click here to switch to the map view.

The map label for this gene is dapE [H]

Identifier: 73542960

GI number: 73542960

Start: 3583108

End: 3584376

Strand: Reverse

Name: dapE [H]

Synonym: Reut_A3277

Alternate gene names: 73542960

Gene position: 3584376-3583108 (Counterclockwise)

Preceding gene: 73542961

Following gene: 73542958

Centisome position: 94.16

GC content: 65.88

Gene sequence:

>1269_bases
ATGACCGCACGAGACAATATGAACCCGATCGAAACCACGCTGACGACGTTCATCGACCAGCATCGCCCGCAACAGGAAGC
CTTCCTGGCAGAGCTGGTCAAGGTCCCCTCCGACAACCCGGCGGGCGACTGCGATGCCCACGGCAAGCGCGCCAAGGAAC
TGCTGGAAGGTCTGGGCTTCACCGTCGAAGCGCACAAGGTGCCTGAAGCGCAAGTGAAGGCCGCCGGCATGATCAGCGCG
ACCAACCTGGTCGTGCGCAAGCAATTCGGCACGGGCGGCCCGGTCATCGCCATGAACGCCCACGGCGACGTGGTGCCTCC
GGGCCTGGGCTGGACCAAGGATCCGTACGGCGGCGAGATTGCGGACAGCGAGCACGGCCCGGTCATGTACGGCCGCGGCG
TGGCGGTGTCGAAGTCGGACTTCGCGACCTACACCTACGCCGTGCTGGCGCTGATGGAAGCCGAGAAGCAGGGCGCGAAG
ATCAACGGTGCGGTCGAGCTGCAGTTCACCTATGACGAGGAAACCGGCGGCGACATCGGTCCCAAGTTCCTGCTCGACAA
CAACCTGAGCAAGGCCGACTACGCGATTTCGGCCGGCTTCTCGTACGGCATCACGTCGTCGCACAACGGCTGCCTGCACG
TCGAAGTGACGGTCAAGGGCAAGCAAGGCCACGCCGCCATGCCGCACACCGGCGTCGACGCCATCGAAGCCGCCACGCAC
ATCCTGCAAGCCATCTACGGCTTGCGCGCGGAGCTGGCCACGCGCAAGTCCAAGGTGCCGGGCATCGACACCGCTACGCT
GAACGTGGGCCTGATCAAGGGCGGCATCAACACCAACGTGGTGCCGGACCTGGTGACCTTCCGCGTCGACCGCCGCATGA
TCCCCGAGGAAATCGGCTTTGACGCCGAAGGCGAAATCCGCGCCGTGGTGGAAAAGGCCGCGAAGGACCGTCCGGGCATC
GAGGTGAAGGTCGAGCGCATCATCCTGGCCGAGCCGCTGTCGGAACTGCCGGGCGTGGAAAAGCTGATCGGCGCGCTGAA
GAGCCGTGCCGAGTCCGTGTTCGGCGTGGAGATCCCGGTGCAAGGCGTGCCGCTGTACACCGACGCGCGCCACTACACCA
AGCGCGGCATCCCGACGGTGCTGTACGGCGCCGGCCCGCGCACGCTGATGGAAGCACGCGGCCACAACTCGGACGAGAAC
CTGCGCCTCAACGACCTGAACCGCGCGACCAAGGTGGTGGCGCTGGCGCTGTCGGACCTGATGGCGTAA

Upstream 100 bases:

>100_bases
TCCGCGCGCGTGTTCAGCGATTTCGTCGAGCACTTCCAGCGGCAGCAATAAGGCTGTCCCCCGAACGCTTCCTGTTACTA
AGATTCCCAGAAAACAAGCC

Downstream 100 bases:

>100_bases
GGCTTCGGCATTTCATCGCGAAGTCCGGAGGGCCCGCAGCAATGCGGGTCTTTTCTTTTGTAGATATCACTCCGTCTCGA
CGGCCAGCGCCACCAGCCAG

Product: peptidase M20:peptidase dimerisation

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTARDNMNPIETTLTTFIDQHRPQQEAFLAELVKVPSDNPAGDCDAHGKRAKELLEGLGFTVEAHKVPEAQVKAAGMISA
TNLVVRKQFGTGGPVIAMNAHGDVVPPGLGWTKDPYGGEIADSEHGPVMYGRGVAVSKSDFATYTYAVLALMEAEKQGAK
INGAVELQFTYDEETGGDIGPKFLLDNNLSKADYAISAGFSYGITSSHNGCLHVEVTVKGKQGHAAMPHTGVDAIEAATH
ILQAIYGLRAELATRKSKVPGIDTATLNVGLIKGGINTNVVPDLVTFRVDRRMIPEEIGFDAEGEIRAVVEKAAKDRPGI
EVKVERIILAEPLSELPGVEKLIGALKSRAESVFGVEIPVQGVPLYTDARHYTKRGIPTVLYGAGPRTLMEARGHNSDEN
LRLNDLNRATKVVALALSDLMA

Sequences:

>Translated_422_residues
MTARDNMNPIETTLTTFIDQHRPQQEAFLAELVKVPSDNPAGDCDAHGKRAKELLEGLGFTVEAHKVPEAQVKAAGMISA
TNLVVRKQFGTGGPVIAMNAHGDVVPPGLGWTKDPYGGEIADSEHGPVMYGRGVAVSKSDFATYTYAVLALMEAEKQGAK
INGAVELQFTYDEETGGDIGPKFLLDNNLSKADYAISAGFSYGITSSHNGCLHVEVTVKGKQGHAAMPHTGVDAIEAATH
ILQAIYGLRAELATRKSKVPGIDTATLNVGLIKGGINTNVVPDLVTFRVDRRMIPEEIGFDAEGEIRAVVEKAAKDRPGI
EVKVERIILAEPLSELPGVEKLIGALKSRAESVFGVEIPVQGVPLYTDARHYTKRGIPTVLYGAGPRTLMEARGHNSDEN
LRLNDLNRATKVVALALSDLMA
>Mature_421_residues
TARDNMNPIETTLTTFIDQHRPQQEAFLAELVKVPSDNPAGDCDAHGKRAKELLEGLGFTVEAHKVPEAQVKAAGMISAT
NLVVRKQFGTGGPVIAMNAHGDVVPPGLGWTKDPYGGEIADSEHGPVMYGRGVAVSKSDFATYTYAVLALMEAEKQGAKI
NGAVELQFTYDEETGGDIGPKFLLDNNLSKADYAISAGFSYGITSSHNGCLHVEVTVKGKQGHAAMPHTGVDAIEAATHI
LQAIYGLRAELATRKSKVPGIDTATLNVGLIKGGINTNVVPDLVTFRVDRRMIPEEIGFDAEGEIRAVVEKAAKDRPGIE
VKVERIILAEPLSELPGVEKLIGALKSRAESVFGVEIPVQGVPLYTDARHYTKRGIPTVLYGAGPRTLMEARGHNSDENL
RLNDLNRATKVVALALSDLMA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790395, Length=355, Percent_Identity=26.4788732394366, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538642, Length=322, Percent_Identity=27.3291925465839, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356353, Length=418, Percent_Identity=24.6411483253589, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24649206, Length=418, Percent_Identity=25.1196172248804, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24649210, Length=410, Percent_Identity=25.609756097561, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24649202, Length=340, Percent_Identity=28.2352941176471, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24649204, Length=340, Percent_Identity=28.2352941176471, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 45137; Mature: 45006

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARDNMNPIETTLTTFIDQHRPQQEAFLAELVKVPSDNPAGDCDAHGKRAKELLEGLGF
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCC
TVEAHKVPEAQVKAAGMISATNLVVRKQFGTGGPVIAMNAHGDVVPPGLGWTKDPYGGEI
EEEECCCCHHHHHHCCCEEHHHEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
ADSEHGPVMYGRGVAVSKSDFATYTYAVLALMEAEKQGAKINGAVELQFTYDEETGGDIG
CCCCCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHCCCEECCEEEEEEEECCCCCCCCC
PKFLLDNNLSKADYAISAGFSYGITSSHNGCLHVEVTVKGKQGHAAMPHTGVDAIEAATH
CEEEECCCCCCCCEEEECCCEECCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHH
ILQAIYGLRAELATRKSKVPGIDTATLNVGLIKGGINTNVVPDLVTFRVDRRMIPEEIGF
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCHHHHCC
DAEGEIRAVVEKAAKDRPGIEVKVERIILAEPLSELPGVEKLIGALKSRAESVFGVEIPV
CCCCHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHCCCHHHHHHHHHHHHHHEEEEECCC
QGVPLYTDARHYTKRGIPTVLYGAGPRTLMEARGHNSDENLRLNDLNRATKVVALALSDL
CCCCEEECCHHHHHCCCCEEEECCCCHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHH
MA
CC
>Mature Secondary Structure 
TARDNMNPIETTLTTFIDQHRPQQEAFLAELVKVPSDNPAGDCDAHGKRAKELLEGLGF
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCC
TVEAHKVPEAQVKAAGMISATNLVVRKQFGTGGPVIAMNAHGDVVPPGLGWTKDPYGGEI
EEEECCCCHHHHHHCCCEEHHHEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
ADSEHGPVMYGRGVAVSKSDFATYTYAVLALMEAEKQGAKINGAVELQFTYDEETGGDIG
CCCCCCCEEEECCEEECCCCHHHHHHHHHHHHHHHHCCCEECCEEEEEEEECCCCCCCCC
PKFLLDNNLSKADYAISAGFSYGITSSHNGCLHVEVTVKGKQGHAAMPHTGVDAIEAATH
CEEEECCCCCCCCEEEECCCEECCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHH
ILQAIYGLRAELATRKSKVPGIDTATLNVGLIKGGINTNVVPDLVTFRVDRRMIPEEIGF
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCHHHHCC
DAEGEIRAVVEKAAKDRPGIEVKVERIILAEPLSELPGVEKLIGALKSRAESVFGVEIPV
CCCCHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHCCCHHHHHHHHHHHHHHEEEEECCC
QGVPLYTDARHYTKRGIPTVLYGAGPRTLMEARGHNSDENLRLNDLNRATKVVALALSDL
CCCCEEECCHHHHHCCCCEEEECCCCHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHH
MA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA