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The map label for this gene is azoR2

Identifier: 73542956

GI number: 73542956

Start: 3580922

End: 3581542

Strand: Reverse

Name: azoR2

Synonym: Reut_A3273

Alternate gene names: 73542956

Gene position: 3581542-3580922 (Counterclockwise)

Preceding gene: 73542957

Following gene: 73542954

Centisome position: 94.09

GC content: 66.34

Gene sequence:

>621_bases
ATGCCCAAGCTCCTTTACCTGAGCGTGAGTCCGCGCGCCGAAAATTCCTATAGCCGGCAGGCCGGCGCTCGCATGATCGC
GTGGCTGGAACGCCGGCACGGGCCGCTGACCGTGATCGATCGCGACCTTGCCGCCGACCCGGTGCCTCATATCGACGGCG
CCATGGCCCGCGCAAGCCTGATGCCGGCTGCGGACCGCGGTCCCGCCGAGCATGCTGCGCTGGCGTTGTCCGAGACGCTG
ATTGGTGAACTGGAGGCGGCAGATATCGTGCTGATCTCCACGCCGATGCACAACTTCACGGTGCCGTCCGCGCTCAAGGC
CTGGATCGACCATGTGGTGCGATCCAACCGGACGTTTCGCAGTACGCCTGCCGGCAAGGTCGGCCTGCTGGCGGACCGCC
CGGTGCTGGCCGTGGTGTCCTGCGGCGGACCGTTCCATGACGGGCCGGGCAGCCAGCGCGACATGATGACGCCTTACCTG
CAGTATGTATTTGGCTCGGTCGGCATCACGCAGGTCGAAGTGGTGCGCATGGAGAATATGGCGCGCGGCGAGGATTTCGT
CGCACGCGGCTTTGAGCGGCTCAATGCGTGGACGGGGAGCCTGGCAATCCGGGCAGCCTGA

Upstream 100 bases:

>100_bases
TGCTCGATACGCCGCTCACCAACGTGCTTGGCCACCGCTTCTATTACCGCAACGGCCTGCTCGCCTCGGCGCTGCGTTTC
AACATCGTTCTGGATTGACC

Downstream 100 bases:

>100_bases
ACGGCTACTCGGCCAGCGTCCCGTTGCCAGCGCCAAGCGGCCGCTGCATCAGCACCGTATCGATCCACTGCCCGTGCTTG
AAGCCGACCGCTTCGAGCCG

Product: (Acyl-carrier protein) phosphodiesterase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 2; FMN-dependent NADH-azo compound oxidoreductase 2

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MPKLLYLSVSPRAENSYSRQAGARMIAWLERRHGPLTVIDRDLAADPVPHIDGAMARASLMPAADRGPAEHAALALSETL
IGELEAADIVLISTPMHNFTVPSALKAWIDHVVRSNRTFRSTPAGKVGLLADRPVLAVVSCGGPFHDGPGSQRDMMTPYL
QYVFGSVGITQVEVVRMENMARGEDFVARGFERLNAWTGSLAIRAA

Sequences:

>Translated_206_residues
MPKLLYLSVSPRAENSYSRQAGARMIAWLERRHGPLTVIDRDLAADPVPHIDGAMARASLMPAADRGPAEHAALALSETL
IGELEAADIVLISTPMHNFTVPSALKAWIDHVVRSNRTFRSTPAGKVGLLADRPVLAVVSCGGPFHDGPGSQRDMMTPYL
QYVFGSVGITQVEVVRMENMARGEDFVARGFERLNAWTGSLAIRAA
>Mature_205_residues
PKLLYLSVSPRAENSYSRQAGARMIAWLERRHGPLTVIDRDLAADPVPHIDGAMARASLMPAADRGPAEHAALALSETLI
GELEAADIVLISTPMHNFTVPSALKAWIDHVVRSNRTFRSTPAGKVGLLADRPVLAVVSCGGPFHDGPGSQRDMMTPYLQ
YVFGSVGITQVEVVRMENMARGEDFVARGFERLNAWTGSLAIRAA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=189, Percent_Identity=37.5661375661376, Blast_Score=124, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR2_CUPPJ (Q46W52)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_297476.1
- ProteinModelPortal:   Q46W52
- SMR:   Q46W52
- GeneID:   3609074
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A3273
- NMPDR:   fig|264198.3.peg.3761
- HOGENOM:   HBG565834
- OMA:   IGTPMNN
- ProtClustDB:   CLSK939675
- BioCyc:   REUT264198:REUT_A3273-MONOMER
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 22301; Mature: 22170

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLLYLSVSPRAENSYSRQAGARMIAWLERRHGPLTVIDRDLAADPVPHIDGAMARASL
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHC
MPAADRGPAEHAALALSETLIGELEAADIVLISTPMHNFTVPSALKAWIDHVVRSNRTFR
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCC
STPAGKVGLLADRPVLAVVSCGGPFHDGPGSQRDMMTPYLQYVFGSVGITQVEVVRMENM
CCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHEEHHHHHHH
ARGEDFVARGFERLNAWTGSLAIRAA
CCCHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
PKLLYLSVSPRAENSYSRQAGARMIAWLERRHGPLTVIDRDLAADPVPHIDGAMARASL
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHC
MPAADRGPAEHAALALSETLIGELEAADIVLISTPMHNFTVPSALKAWIDHVVRSNRTFR
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCC
STPAGKVGLLADRPVLAVVSCGGPFHDGPGSQRDMMTPYLQYVFGSVGITQVEVVRMENM
CCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHEEHHHHHHH
ARGEDFVARGFERLNAWTGSLAIRAA
CCCHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA