Click here to switch to the map view.

The map label for this gene is tag [H]

Identifier: 73542926

GI number: 73542926

Start: 3556911

End: 3557537

Strand: Direct

Name: tag [H]

Synonym: Reut_A3242

Alternate gene names: 73542926

Gene position: 3556911-3557537 (Clockwise)

Preceding gene: 73542925

Following gene: 73542930

Centisome position: 93.44

GC content: 66.35

Gene sequence:

>627_bases
ATGACACGAAAACAGGAAGCCGCCGCCGAACGCGTGCGCTGCGGCTGGTGCGGCACGCTGGAAGACTATTGCCACTACCA
CGATAACGAATGGGGCTTTCCGGTCGACGACGACCGGCGCCTGTTCGAGAAACTCTGCCTCGAAGGCTTCCAGGCCGGCC
TGAGCTGGCTGACCATCCTGCGCAAGCGCGAGGCCTTCCGCAAGGCGTTCGCGAACTTTGACATCGAGAAGGTCGCTCGC
TTTGGCGAGCGCGACATCCAGCGCCTGCTGGCCGATGCCGGCATCGTGCGCCACCGCGGCAAGATCGAGGCGGCTATCAA
CAATGCGCAGCGCGCTGCCGAGTTGCTGGAAACGGAGTCGTCGCTGGCGGCCTACTTCTGGCGCTACGAGCCCGACCCGG
CGAGCCGGCCCACGGTACTGACGCCCGAGGTACTGCGGACCATGGCGACGTCGGCGGAATCGGCGGCGCTGTCCAAGGAC
TTGAAGAAGCGCGGCTGGCGCTTCGTCGGACCGACCACGATGTACGCGCTGATGCAGGCGATGGGCCTCGTCAACGACCA
CCAGGACGGCTGCTGGACCCGGACCGAAGCACTGCGCGCGCGCAAGGCGTTCAAGGTGCCGCGCTAG

Upstream 100 bases:

>100_bases
GCCTTCCGCAAACCGCGCGCCCGGTGCATGATCACCGGCGGACGCCGGTGCCACGCAAGTATGGCCGGCCATCACCAACC
CGGGCCATGGAAAGGCACGC

Downstream 100 bases:

>100_bases
GCGGCGCGCTCAAGGCAGGCAGAACGTCACCGACGGCACGTCGTCCACTGTCACACGCGCGGGCAGCGTGTACACGCGTT
CCATGTTCTGCGGCGTCAGC

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTRKQEAAAERVRCGWCGTLEDYCHYHDNEWGFPVDDDRRLFEKLCLEGFQAGLSWLTILRKREAFRKAFANFDIEKVAR
FGERDIQRLLADAGIVRHRGKIEAAINNAQRAAELLETESSLAAYFWRYEPDPASRPTVLTPEVLRTMATSAESAALSKD
LKKRGWRFVGPTTMYALMQAMGLVNDHQDGCWTRTEALRARKAFKVPR

Sequences:

>Translated_208_residues
MTRKQEAAAERVRCGWCGTLEDYCHYHDNEWGFPVDDDRRLFEKLCLEGFQAGLSWLTILRKREAFRKAFANFDIEKVAR
FGERDIQRLLADAGIVRHRGKIEAAINNAQRAAELLETESSLAAYFWRYEPDPASRPTVLTPEVLRTMATSAESAALSKD
LKKRGWRFVGPTTMYALMQAMGLVNDHQDGCWTRTEALRARKAFKVPR
>Mature_207_residues
TRKQEAAAERVRCGWCGTLEDYCHYHDNEWGFPVDDDRRLFEKLCLEGFQAGLSWLTILRKREAFRKAFANFDIEKVARF
GERDIQRLLADAGIVRHRGKIEAAINNAQRAAELLETESSLAAYFWRYEPDPASRPTVLTPEVLRTMATSAESAALSKDL
KKRGWRFVGPTTMYALMQAMGLVNDHQDGCWTRTEALRARKAFKVPR

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=180, Percent_Identity=49.4444444444444, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 23888; Mature: 23757

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKQEAAAERVRCGWCGTLEDYCHYHDNEWGFPVDDDRRLFEKLCLEGFQAGLSWLTIL
CCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RKREAFRKAFANFDIEKVARFGERDIQRLLADAGIVRHRGKIEAAINNAQRAAELLETES
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SLAAYFWRYEPDPASRPTVLTPEVLRTMATSAESAALSKDLKKRGWRFVGPTTMYALMQA
HHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MGLVNDHQDGCWTRTEALRARKAFKVPR
HHHHCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRKQEAAAERVRCGWCGTLEDYCHYHDNEWGFPVDDDRRLFEKLCLEGFQAGLSWLTIL
CCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RKREAFRKAFANFDIEKVARFGERDIQRLLADAGIVRHRGKIEAAINNAQRAAELLETES
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SLAAYFWRYEPDPASRPTVLTPEVLRTMATSAESAALSKDLKKRGWRFVGPTTMYALMQA
HHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
MGLVNDHQDGCWTRTEALRARKAFKVPR
HHHHCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]