Click here to switch to the map view.

The map label for this gene is emrB [H]

Identifier: 73542930

GI number: 73542930

Start: 3560375

End: 3561910

Strand: Direct

Name: emrB [H]

Synonym: Reut_A3247

Alternate gene names: 73542930

Gene position: 3560375-3561910 (Clockwise)

Preceding gene: 73542926

Following gene: 73542931

Centisome position: 93.53

GC content: 64.45

Gene sequence:

>1536_bases
ATGTCCTCTCTAGCCAAGCCGGCCGGCGCCGACACGCCGCTGAACCGGCCCATGATCACTGTCTCGATCATGCTGGCAAC
GCTGATCCAGACGCTGGACAGCACCATTGCCAACGTCGCGCTGCCGCACATGCAGGGCACGCTCTCCGCATCGCAGGACG
AGATCACGTGGGTGCTGACGTCCTACATCGTCGCCGCGGCCATCGCCACGCCACTGACGGGCTGGTTGTCCGACCGGCTC
GGCGTGAAGCGGCTGTTGCTGATCGCCATCGGTGGCTTCACAGTGGCATCGGCCTTCTGCGGGCTGGCCGAGACGCTGCC
GCAAATGGTCGCCGCGCGCCTGCTGCAAGGCATCTTCGGCGCATCGCTGGTGCCGCTGTCGCAGTCGATCCTGCTCGACA
TCAACCCGCCGTCGCGCCAGGGACAGGCCATGGCGGTGTGGGGCATGGGCGTGATGGTTGGTCCAATCCTCGGGCCGACA
CTGGGCGGATGGCTGACCGACAGCTACAACTGGCGCTGGGTGTTCTTCATCAACGTGCCGATCGGTGCGTTTGCCATGTT
CGGTGTCATGTCGTTCCTGCCCGCCAGGGCGCCGCGAAGGGAGATCAAGTTCGATGCGTTCGGCTTTGCCACGCTGAGCC
TGGCCATCGGTGCGCTGCAGGCCATGCTCGACCGCGGCGAGCAGCTCGACTGGTTCAGCTCGCCGGAAATCCGCATCGAA
GCGCTGCTCGCGGTGATCAGCTTTGCCTATTTCATCGTGCACACGGCGACGGCAGGCGCGAAGTCGTTCTTCCGGTTCGA
ACTGCTGAAGGACCGCAACTTCGCCACGGGCACGTGTTTCATCTTCGTCATCGGGGCGGTCATGTACGCCACGCGTGCAC
TGCTGCCGCCGATGCTGCAAGGGCTGATGCAGTATCCCGTGGCGACCACTGGTCTTGTCACCGCGCCGAGCGGCGCCGGC
ACCATGGTGGCGATGCTGCTCGCCGGCCGTTTGCTGCGCCGCATTGATGCGCGGCTGCTGCTACTGTCGGGCTTCCTGAT
CTCGGCGTTCGCGCTGTGGCAGATGATGCACTACACCATCGTGCTGTCGATGTCCGATATCGTCTGGCCCGGCGTGATCC
AGGGCTTCGGTCTGGGGCTCGTGTTTGTGCCACTGAGCGCGCTGACGTTCTCCACGCTGCGGCCGGATCTGCGCGCCGAT
GGCACCGCGACCTACAGCCTGGTGCGCAATATCGGCAGCAGCATCGGCATCTCGGTCATCCAGGCGCTGATGACACGCAA
CACGCAAGTCGCGCACGCCGACCTGGCCGCGCATGTCAGCGTCTACAACCCGAACCTGCAATCGATGATCGCAAACGGCT
CGCGCACCGACCTGATGGCGCTGAACCATGCAATCACCCAGCAGGCGTCGATGATCGCCTACCTCAACGACTTCAAGGCG
ATGTTCATCGCCACGCTGCTGGTCGTACCGCTGATCCTGCTCGTGCGGCCTGTCAGGCAAGCGCCGACAGACCTGGCCCA
TGCTGCGATGGATTGA

Upstream 100 bases:

>100_bases
CAGTGAAGAGGTCGGCACACCGGGATGACGCGCCCTGTCACACCACCCCACCCGGCCATATAATAAGCACGCTTATCAAT
AAACGGCCCCCGCGCGCTTC

Downstream 100 bases:

>100_bases
CGATGGACCGAGAAGCGGCCGTTGTATGCGCGAGACGACACAGGTGTATGGAGACGTAACAGCACTGGCGAACTTGCTGT
GACGTACTACGGTACAAATT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSSLAKPAGADTPLNRPMITVSIMLATLIQTLDSTIANVALPHMQGTLSASQDEITWVLTSYIVAAAIATPLTGWLSDRL
GVKRLLLIAIGGFTVASAFCGLAETLPQMVAARLLQGIFGASLVPLSQSILLDINPPSRQGQAMAVWGMGVMVGPILGPT
LGGWLTDSYNWRWVFFINVPIGAFAMFGVMSFLPARAPRREIKFDAFGFATLSLAIGALQAMLDRGEQLDWFSSPEIRIE
ALLAVISFAYFIVHTATAGAKSFFRFELLKDRNFATGTCFIFVIGAVMYATRALLPPMLQGLMQYPVATTGLVTAPSGAG
TMVAMLLAGRLLRRIDARLLLLSGFLISAFALWQMMHYTIVLSMSDIVWPGVIQGFGLGLVFVPLSALTFSTLRPDLRAD
GTATYSLVRNIGSSIGISVIQALMTRNTQVAHADLAAHVSVYNPNLQSMIANGSRTDLMALNHAITQQASMIAYLNDFKA
MFIATLLVVPLILLVRPVRQAPTDLAHAAMD

Sequences:

>Translated_511_residues
MSSLAKPAGADTPLNRPMITVSIMLATLIQTLDSTIANVALPHMQGTLSASQDEITWVLTSYIVAAAIATPLTGWLSDRL
GVKRLLLIAIGGFTVASAFCGLAETLPQMVAARLLQGIFGASLVPLSQSILLDINPPSRQGQAMAVWGMGVMVGPILGPT
LGGWLTDSYNWRWVFFINVPIGAFAMFGVMSFLPARAPRREIKFDAFGFATLSLAIGALQAMLDRGEQLDWFSSPEIRIE
ALLAVISFAYFIVHTATAGAKSFFRFELLKDRNFATGTCFIFVIGAVMYATRALLPPMLQGLMQYPVATTGLVTAPSGAG
TMVAMLLAGRLLRRIDARLLLLSGFLISAFALWQMMHYTIVLSMSDIVWPGVIQGFGLGLVFVPLSALTFSTLRPDLRAD
GTATYSLVRNIGSSIGISVIQALMTRNTQVAHADLAAHVSVYNPNLQSMIANGSRTDLMALNHAITQQASMIAYLNDFKA
MFIATLLVVPLILLVRPVRQAPTDLAHAAMD
>Mature_510_residues
SSLAKPAGADTPLNRPMITVSIMLATLIQTLDSTIANVALPHMQGTLSASQDEITWVLTSYIVAAAIATPLTGWLSDRLG
VKRLLLIAIGGFTVASAFCGLAETLPQMVAARLLQGIFGASLVPLSQSILLDINPPSRQGQAMAVWGMGVMVGPILGPTL
GGWLTDSYNWRWVFFINVPIGAFAMFGVMSFLPARAPRREIKFDAFGFATLSLAIGALQAMLDRGEQLDWFSSPEIRIEA
LLAVISFAYFIVHTATAGAKSFFRFELLKDRNFATGTCFIFVIGAVMYATRALLPPMLQGLMQYPVATTGLVTAPSGAGT
MVAMLLAGRLLRRIDARLLLLSGFLISAFALWQMMHYTIVLSMSDIVWPGVIQGFGLGLVFVPLSALTFSTLRPDLRADG
TATYSLVRNIGSSIGISVIQALMTRNTQVAHADLAAHVSVYNPNLQSMIANGSRTDLMALNHAITQQASMIAYLNDFKAM
FIATLLVVPLILLVRPVRQAPTDLAHAAMD

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=437, Percent_Identity=37.9862700228833, Blast_Score=301, Evalue=7e-83,
Organism=Escherichia coli, GI1788710, Length=492, Percent_Identity=35.7723577235772, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1790195, Length=410, Percent_Identity=28.0487804878049, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI87081983, Length=427, Percent_Identity=27.1662763466042, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788392, Length=395, Percent_Identity=29.620253164557, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI87082320, Length=177, Percent_Identity=27.683615819209, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790146, Length=151, Percent_Identity=28.476821192053, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=391, Percent_Identity=26.8542199488491, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=136, Percent_Identity=31.6176470588235, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=148, Percent_Identity=27.027027027027, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=336, Percent_Identity=26.7857142857143, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=307, Percent_Identity=23.7785016286645, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54942; Mature: 54810

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLAKPAGADTPLNRPMITVSIMLATLIQTLDSTIANVALPHMQGTLSASQDEITWVLT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
SYIVAAAIATPLTGWLSDRLGVKRLLLIAIGGFTVASAFCGLAETLPQMVAARLLQGIFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASLVPLSQSILLDINPPSRQGQAMAVWGMGVMVGPILGPTLGGWLTDSYNWRWVFFINVP
CHHHCCCCEEEEECCCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECC
IGAFAMFGVMSFLPARAPRREIKFDAFGFATLSLAIGALQAMLDRGEQLDWFSSPEIRIE
HHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
ALLAVISFAYFIVHTATAGAKSFFRFELLKDRNFATGTCFIFVIGAVMYATRALLPPMLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLMQYPVATTGLVTAPSGAGTMVAMLLAGRLLRRIDARLLLLSGFLISAFALWQMMHYTI
HHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLSMSDIVWPGVIQGFGLGLVFVPLSALTFSTLRPDLRADGTATYSLVRNIGSSIGISVI
HHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHH
QALMTRNTQVAHADLAAHVSVYNPNLQSMIANGSRTDLMALNHAITQQASMIAYLNDFKA
HHHHHCCCCHHHHHHHHEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MFIATLLVVPLILLVRPVRQAPTDLAHAAMD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
SSLAKPAGADTPLNRPMITVSIMLATLIQTLDSTIANVALPHMQGTLSASQDEITWVLT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
SYIVAAAIATPLTGWLSDRLGVKRLLLIAIGGFTVASAFCGLAETLPQMVAARLLQGIFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASLVPLSQSILLDINPPSRQGQAMAVWGMGVMVGPILGPTLGGWLTDSYNWRWVFFINVP
CHHHCCCCEEEEECCCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECC
IGAFAMFGVMSFLPARAPRREIKFDAFGFATLSLAIGALQAMLDRGEQLDWFSSPEIRIE
HHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
ALLAVISFAYFIVHTATAGAKSFFRFELLKDRNFATGTCFIFVIGAVMYATRALLPPMLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLMQYPVATTGLVTAPSGAGTMVAMLLAGRLLRRIDARLLLLSGFLISAFALWQMMHYTI
HHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLSMSDIVWPGVIQGFGLGLVFVPLSALTFSTLRPDLRADGTATYSLVRNIGSSIGISVI
HHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHH
QALMTRNTQVAHADLAAHVSVYNPNLQSMIANGSRTDLMALNHAITQQASMIAYLNDFKA
HHHHHCCCCHHHHHHHHEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MFIATLLVVPLILLVRPVRQAPTDLAHAAMD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]