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The map label for this gene is livK [C]

Identifier: 73542682

GI number: 73542682

Start: 3292137

End: 3293429

Strand: Direct

Name: livK [C]

Synonym: Reut_A2998

Alternate gene names: 73542682

Gene position: 3292137-3293429 (Clockwise)

Preceding gene: 73542681

Following gene: 73542685

Centisome position: 86.49

GC content: 65.27

Gene sequence:

>1293_bases
ATGCAGTCCACCCCGTCAACGCACAGATCAACCGGCGCGCGCAAGCGTCCCCTGGCCCGCCTGCTGGCCACCACGGCCTT
GGCCCTGCTGCCAGCATTGGCCGCGGCTCAGGACGCGCCAATCAAAGTCGCGTTTATCGACCAGCTCTCCGGTCCGCTGG
CCAATGTCGGCGAGATCTTCCAGGCCAACCTGAAGTTCGCGATCGACGATGCCAATGCGCAGGGCGGCGTACTCGGCCGC
AAGTATGAGCTGACGAGCTACGACAACAAGCTGTCCGCGCAGGATAGTCTGTCGGCGCTGCAAAGTGCGATCGATGCCGG
GGTAAAGGTCGTGTTCACGGGCGGATCGGGTTCTTCGGTGGTCACGGCACTGGTCGAAGCCGCCACGCGCCACAACGAGC
GCAACCCCGATCGACGCATCCTGATCGTCAACTACGCGTCGATCGATCCGGACCTGACCGGCGCGCGCTGCAGCTTCTGG
CATTTCGCGACTGAGGCCAACACGTCCATGAAGATGCGCGCGCTGGCGGACTTCACGCGCGAGCAGAAGGACATCCACAA
GCTTTACTTGCTGAACCAGGACTATGCGCACGGGCGGGTATGGGCGGCGCTGGGCCAGCAGATGATCGCCACTGCGCGTC
CGGACACGCAGTTCGTCGGCGCCACGCTGCACCCGCTCGGCAAGGTCAAGGACTTCGCACCCTATGTCAGCAAGATCAAG
GCGAGTGGCGCCGACACCATCGTCACGGGCAACTGGGGCCAGGACCTGAGCCTGCTGATCAAGGCCGCCGGCGACATCGG
CTATGACCCGCGCTACCTGAACCACAGCGGCGGCGGCGCGCCCGGTACGGTGCTGGCGGTGTCGCAGGCGAAAAGCGGCA
AGCTCACATGGGTGTCGGAATGGCTGCCCAATACGGAAGATCCGGCACTGTCCGCGCTCGCGGCGCGCGTGAAGCAGACC
GCCGCCGGCGAATACTTTACGCCGCGCGTGCTGGTGTCGGTGCAGCTCGTGACCGAGGCGATACGCCGCGCTGGCACCGA
TGAGCCGACGAAAATCGCGATGGCACTCGAAGGCATGAAGATGAAGACGGCGCTGGGCGAGGTCACGATGCGCAAGAGCG
ATCATCAGCTACTGCTGCCGCAGGTGGTGTCGACCGTGGCGCCTGTGGACGGCAAGACCGTCAAGGTCGGCGCCGAAGGC
ACGCAGTACGGCTTGCGCCTGAACAGCGTGACGCCGGCACAGGCACTCGACTTGCCGTCCGCCTGCCACATGAAGCGGCC
TACCGGCGTCTGA

Upstream 100 bases:

>100_bases
GGCGCACTGGCGAGGACCGCCGCTTCATCGACACCTGGTAATAACGCGCCGGGAACACGGCGCCACCCGGATTCTTCATC
GACACAGGAGGAGACACCTG

Downstream 100 bases:

>100_bases
TTTGGCCCGTGTCCGGTCAGTGGCCCAACTTGCCCACGCCCGATGAGTGATCTTCGGCGTTTAGCGTACCGGTGTTCACC
GCCGGCGGGGCCAGCTTGCT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MQSTPSTHRSTGARKRPLARLLATTALALLPALAAAQDAPIKVAFIDQLSGPLANVGEIFQANLKFAIDDANAQGGVLGR
KYELTSYDNKLSAQDSLSALQSAIDAGVKVVFTGGSGSSVVTALVEAATRHNERNPDRRILIVNYASIDPDLTGARCSFW
HFATEANTSMKMRALADFTREQKDIHKLYLLNQDYAHGRVWAALGQQMIATARPDTQFVGATLHPLGKVKDFAPYVSKIK
ASGADTIVTGNWGQDLSLLIKAAGDIGYDPRYLNHSGGGAPGTVLAVSQAKSGKLTWVSEWLPNTEDPALSALAARVKQT
AAGEYFTPRVLVSVQLVTEAIRRAGTDEPTKIAMALEGMKMKTALGEVTMRKSDHQLLLPQVVSTVAPVDGKTVKVGAEG
TQYGLRLNSVTPAQALDLPSACHMKRPTGV

Sequences:

>Translated_430_residues
MQSTPSTHRSTGARKRPLARLLATTALALLPALAAAQDAPIKVAFIDQLSGPLANVGEIFQANLKFAIDDANAQGGVLGR
KYELTSYDNKLSAQDSLSALQSAIDAGVKVVFTGGSGSSVVTALVEAATRHNERNPDRRILIVNYASIDPDLTGARCSFW
HFATEANTSMKMRALADFTREQKDIHKLYLLNQDYAHGRVWAALGQQMIATARPDTQFVGATLHPLGKVKDFAPYVSKIK
ASGADTIVTGNWGQDLSLLIKAAGDIGYDPRYLNHSGGGAPGTVLAVSQAKSGKLTWVSEWLPNTEDPALSALAARVKQT
AAGEYFTPRVLVSVQLVTEAIRRAGTDEPTKIAMALEGMKMKTALGEVTMRKSDHQLLLPQVVSTVAPVDGKTVKVGAEG
TQYGLRLNSVTPAQALDLPSACHMKRPTGV
>Mature_430_residues
MQSTPSTHRSTGARKRPLARLLATTALALLPALAAAQDAPIKVAFIDQLSGPLANVGEIFQANLKFAIDDANAQGGVLGR
KYELTSYDNKLSAQDSLSALQSAIDAGVKVVFTGGSGSSVVTALVEAATRHNERNPDRRILIVNYASIDPDLTGARCSFW
HFATEANTSMKMRALADFTREQKDIHKLYLLNQDYAHGRVWAALGQQMIATARPDTQFVGATLHPLGKVKDFAPYVSKIK
ASGADTIVTGNWGQDLSLLIKAAGDIGYDPRYLNHSGGGAPGTVLAVSQAKSGKLTWVSEWLPNTEDPALSALAARVKQT
AAGEYFTPRVLVSVQLVTEAIRRAGTDEPTKIAMALEGMKMKTALGEVTMRKSDHQLLLPQVVSTVAPVDGKTVKVGAEG
TQYGLRLNSVTPAQALDLPSACHMKRPTGV

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=353, Percent_Identity=23.7960339943343, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI48994941, Length=359, Percent_Identity=22.5626740947075, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45825; Mature: 45825

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTPSTHRSTGARKRPLARLLATTALALLPALAAAQDAPIKVAFIDQLSGPLANVGEIF
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCHHHHHHHH
QANLKFAIDDANAQGGVLGRKYELTSYDNKLSAQDSLSALQSAIDAGVKVVFTGGSGSSV
HCCCEEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHH
VTALVEAATRHNERNPDRRILIVNYASIDPDLTGARCSFWHFATEANTSMKMRALADFTR
HHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
EQKDIHKLYLLNQDYAHGRVWAALGQQMIATARPDTQFVGATLHPLGKVKDFAPYVSKIK
HHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHCCHHCHHHHHHHHHHHHHHHH
ASGADTIVTGNWGQDLSLLIKAAGDIGYDPRYLNHSGGGAPGTVLAVSQAKSGKLTWVSE
CCCCCEEEECCCCCCHHHHHHHHCCCCCCCCEECCCCCCCCCEEEEEECCCCCCEEEHHH
WLPNTEDPALSALAARVKQTAAGEYFTPRVLVSVQLVTEAIRRAGTDEPTKIAMALEGMK
HCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHHCCCH
MKTALGEVTMRKSDHQLLLPQVVSTVAPVDGKTVKVGAEGTQYGLRLNSVTPAQALDLPS
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCEECEEECCCCCHHHHCCCH
ACHMKRPTGV
HHCCCCCCCC
>Mature Secondary Structure
MQSTPSTHRSTGARKRPLARLLATTALALLPALAAAQDAPIKVAFIDQLSGPLANVGEIF
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCHHHHHHHH
QANLKFAIDDANAQGGVLGRKYELTSYDNKLSAQDSLSALQSAIDAGVKVVFTGGSGSSV
HCCCEEEEECCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHH
VTALVEAATRHNERNPDRRILIVNYASIDPDLTGARCSFWHFATEANTSMKMRALADFTR
HHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
EQKDIHKLYLLNQDYAHGRVWAALGQQMIATARPDTQFVGATLHPLGKVKDFAPYVSKIK
HHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHCCHHCHHHHHHHHHHHHHHHH
ASGADTIVTGNWGQDLSLLIKAAGDIGYDPRYLNHSGGGAPGTVLAVSQAKSGKLTWVSE
CCCCCEEEECCCCCCHHHHHHHHCCCCCCCCEECCCCCCCCCEEEEEECCCCCCEEEHHH
WLPNTEDPALSALAARVKQTAAGEYFTPRVLVSVQLVTEAIRRAGTDEPTKIAMALEGMK
HCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHHCCCH
MKTALGEVTMRKSDHQLLLPQVVSTVAPVDGKTVKVGAEGTQYGLRLNSVTPAQALDLPS
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCEECEEECCCCCHHHHCCCH
ACHMKRPTGV
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA