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The map label for this gene is ispB [H]

Identifier: 73542643

GI number: 73542643

Start: 3243927

End: 3244904

Strand: Direct

Name: ispB [H]

Synonym: Reut_A2959

Alternate gene names: 73542643

Gene position: 3243927-3244904 (Clockwise)

Preceding gene: 73542638

Following gene: 73542644

Centisome position: 85.22

GC content: 64.83

Gene sequence:

>978_bases
TTGACCCAGCCTTCCGCCACTGCCTTGCTTGCCCCGGTCGCTGCAGACATGCGCGCGGTCGATGCGGTCATCCGTCAACG
GCTGTCTTCTGAAGTTCCACTGATCGAACAGATCGGCGAATACATCATCAGTGCCGGCGGCAAGCGCCTGCGCCCGGTGA
TCCTGCTGATGTCGGCACGCGCGTTTGGCTACGACGGCAACCGCCACCATGAGCTCGCAGCCGTCGTCGAGTTCATCCAC
ACCGCCACGCTGCTGCATGACGATGTGGTCGATGAATCCGAACTGCGCCGCGGCCGCGAGACCGCCAACGCGGTGTTCGG
CAACGCCGCCAGCGTGCTGGTCGGCGACTTCCTTTATTCGCGCGCATTCCAGATGATGGTCGATGCGGGCAGCATGCGAA
TCATGGAGATTCTGTCCAACGCGACCAACGTGATCGCCGAAGGCGAAGTGCTGCAGCTGCTGAACATGCACGATCCCGAC
GTCACGATCGAACGCTACCTGCAAGTGATCCGCTACAAGACGGCCAAGCTGTTCGAGGCCTCCGCCCAGCTTGGCGCGGT
GCTGGCCGGTGCGGACGCCGCCATGGAAGAAGCCGCCGCCGAGTACGGCCGCCGTATCGGCACCGCGTTCCAGCTCATCG
ATGACATGCTCGACTACACCGCGAGTGCCGAGCAGATGGGCAAGAACGCCGGCGACGACCTTCGCGAAGGCAAGCCGACG
CTGCCGCTGCTGCACCTGCTCGAGTACGGCACGCCGGAGCAGCGCGTGCTGGCGCGCGAAGCGATCGTGCAAGGCGGCAC
CGAGCATTTCGACGCAGTGTTCTCGGCCATCCATGCGAGCGGCGCGCTCGATGTCACGTTCGAAGCGGCACGCCGCGAAG
CCGAGGCCGCCGAGAAGGCTGCGCAGCAGTTCCCGGCGTCCGACTGGAAAGACACGCTGATCGCGCTGTGCGAGTTCTCG
CTGCAGCGGCAATCCTGA

Upstream 100 bases:

>100_bases
TAGTCCGCCGCTACCCGCATCCCCCGAACGGGGACAGCCATTGGCGTGAACCACGTTCGCATCGCATATAATCGTCCGGT
TTTGCGTAACGGTGATCATC

Downstream 100 bases:

>100_bases
AATTGCCGCCAGCCTCTTCCCTTCCGGAAGAAACGCTGTTAAAGTTACGTTCTTTGCTGCTGACGCTAACGTGTTAGCAA
CAAAGCAGAGTATTAGCAAC

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTQPSATALLAPVAADMRAVDAVIRQRLSSEVPLIEQIGEYIISAGGKRLRPVILLMSARAFGYDGNRHHELAAVVEFIH
TATLLHDDVVDESELRRGRETANAVFGNAASVLVGDFLYSRAFQMMVDAGSMRIMEILSNATNVIAEGEVLQLLNMHDPD
VTIERYLQVIRYKTAKLFEASAQLGAVLAGADAAMEEAAAEYGRRIGTAFQLIDDMLDYTASAEQMGKNAGDDLREGKPT
LPLLHLLEYGTPEQRVLAREAIVQGGTEHFDAVFSAIHASGALDVTFEAARREAEAAEKAAQQFPASDWKDTLIALCEFS
LQRQS

Sequences:

>Translated_325_residues
MTQPSATALLAPVAADMRAVDAVIRQRLSSEVPLIEQIGEYIISAGGKRLRPVILLMSARAFGYDGNRHHELAAVVEFIH
TATLLHDDVVDESELRRGRETANAVFGNAASVLVGDFLYSRAFQMMVDAGSMRIMEILSNATNVIAEGEVLQLLNMHDPD
VTIERYLQVIRYKTAKLFEASAQLGAVLAGADAAMEEAAAEYGRRIGTAFQLIDDMLDYTASAEQMGKNAGDDLREGKPT
LPLLHLLEYGTPEQRVLAREAIVQGGTEHFDAVFSAIHASGALDVTFEAARREAEAAEKAAQQFPASDWKDTLIALCEFS
LQRQS
>Mature_324_residues
TQPSATALLAPVAADMRAVDAVIRQRLSSEVPLIEQIGEYIISAGGKRLRPVILLMSARAFGYDGNRHHELAAVVEFIHT
ATLLHDDVVDESELRRGRETANAVFGNAASVLVGDFLYSRAFQMMVDAGSMRIMEILSNATNVIAEGEVLQLLNMHDPDV
TIERYLQVIRYKTAKLFEASAQLGAVLAGADAAMEEAAAEYGRRIGTAFQLIDDMLDYTASAEQMGKNAGDDLREGKPTL
PLLHLLEYGTPEQRVLAREAIVQGGTEHFDAVFSAIHASGALDVTFEAARREAEAAEKAAQQFPASDWKDTLIALCEFSL
QRQS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=315, Percent_Identity=31.4285714285714, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI1789578, Length=311, Percent_Identity=57.556270096463, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1786623, Length=259, Percent_Identity=32.8185328185328, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17505681, Length=242, Percent_Identity=35.5371900826446, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319475, Length=221, Percent_Identity=34.841628959276, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6325188, Length=233, Percent_Identity=24.8927038626609, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24651612, Length=299, Percent_Identity=31.7725752508361, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35408; Mature: 35276

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPSATALLAPVAADMRAVDAVIRQRLSSEVPLIEQIGEYIISAGGKRLRPVILLMSAR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
AFGYDGNRHHELAAVVEFIHTATLLHDDVVDESELRRGRETANAVFGNAASVLVGDFLYS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RAFQMMVDAGSMRIMEILSNATNVIAEGEVLQLLNMHDPDVTIERYLQVIRYKTAKLFEA
HHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
SAQLGAVLAGADAAMEEAAAEYGRRIGTAFQLIDDMLDYTASAEQMGKNAGDDLREGKPT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHCCCCC
LPLLHLLEYGTPEQRVLAREAIVQGGTEHFDAVFSAIHASGALDVTFEAARREAEAAEKA
HHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHH
AQQFPASDWKDTLIALCEFSLQRQS
HHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQPSATALLAPVAADMRAVDAVIRQRLSSEVPLIEQIGEYIISAGGKRLRPVILLMSAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
AFGYDGNRHHELAAVVEFIHTATLLHDDVVDESELRRGRETANAVFGNAASVLVGDFLYS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RAFQMMVDAGSMRIMEILSNATNVIAEGEVLQLLNMHDPDVTIERYLQVIRYKTAKLFEA
HHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
SAQLGAVLAGADAAMEEAAAEYGRRIGTAFQLIDDMLDYTASAEQMGKNAGDDLREGKPT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHCCCCC
LPLLHLLEYGTPEQRVLAREAIVQGGTEHFDAVFSAIHASGALDVTFEAARREAEAAEKA
HHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHH
AQQFPASDWKDTLIALCEFSLQRQS
HHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]