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The map label for this gene is pilF [H]

Identifier: 73542644

GI number: 73542644

Start: 3245367

End: 3247127

Strand: Direct

Name: pilF [H]

Synonym: Reut_A2960

Alternate gene names: 73542644

Gene position: 3245367-3247127 (Clockwise)

Preceding gene: 73542643

Following gene: 73542645

Centisome position: 85.26

GC content: 64.22

Gene sequence:

>1761_bases
ATGCACTACCTGGTCCCTGCCATGACACTTGGTCTCGCCCTTGCACAGAGCCGTCGTATCGCCCCCGCGCTGCTCGCCCA
ACTTGAGCAGTCGGCACGCGAAAAGCAGACGCAGCTGATCGACGAGATCGTTGGCACCGGCACCATGACCGCGCACGACG
TCGCACTGTTCGCCGCCGACAAGTATCAGCTTCCACTGCTCGATCTCAGCCAGTACAACATGAACAAGGTGCCGTCCGCG
CTGGCCGGCAACCGCGAATTCCACGCGCACCGCCTGCTGCCGCTGGGGCGGCGCGAGAACCGCCTTGTCGTGGCCATGTC
GGACCCGTCCAACCAGGCGGGACTCGACGCCATCAAGCAGAAGTACAACCTGCCGCTCGACACCGTGGTGGTGGAGCACG
ACAAGCTGATGAAGCATGTGCGTTCCGCCGGCGAAGCGCTGGGCACGCTCAAGGACGTTGCGCCCGGCACGCCCACTGAG
CGCAAGATGATCGAATACGATCCGGTCGCCGCCGCCGCGCAGCGGCCCCGCACGGGCGCCACCAACGACATCGACGATGC
GCCGGTCGTGCGCTTCCTGCAGAAGCTGCTGACCGAAGCCTTCCACCGCGGCGCGTCGGACTTGCACTTCGAGCCGTTCG
AGACCTTCTACCGCATCCGCTTCCGCGTGGACGGCGTGCTGCACGAGGTCGCGCGGCCGCCGCTCGATATTCGCGACAAG
ATCGCCACGCGCATCAAGGTGCTGTCGCGCCTGGACATTTCTGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCT
GATCGCGCTGCCCAAGGACAAGGACAAGGACGGCAAGGACAAGGAAACCGTCGAGAAGGCGGTGGACTTCCGCGTGTCGA
CGCTGCCCACGCTCTTCGGCGAGAAGATCGTGATGCGTATCCTGGAATCATCGTCCGACAAGCTCGATATCGACCAGCTC
GGCTACGAGCCCGAACAGAAGGCGCTGCTGCTGGAAACCATCAAGCGGCCCTACGGCATGATCCTGGTGACCGGGCCGAC
CGGCAGCGGCAAGACCGTGTCGCTCTATACGTTCCTGAACCTGCTGAACCAGGGCGATATCAATATCTCGACCGCTGAGG
ACCCGGCTGAAATCCAGCTGCCCGGCATCAACCAGGTCAACGTCAACGACAAAGCCGGCCTGACCTTTGCCGCGGCGCTG
CGTTCGTTCCTGCGGCAGGATCCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGATATCTCGATCAA
GGCCGCGCAGACAGGCCACCTGGTGCTGTCGACGCTGCACACCAACGATGCGCCGACCACGCTCACGCGCCTGATGAACA
TGGGCGTGGCGCCGTTCAACATCGCATCGAGCGTGCTGCTGATCACGGCGCAGCGCCTGGCGCGGCGGCTGTGCACCTGC
AAGAAGCCCGGCACGATCCCGCGCGAAGCGCTGCTCGACGCCGGGTTCAAGGAACCGCAGCTCGACGGTAGCTGGCAGCC
GTACCATCCGGTCGGCTGCGAGCGCTGCAATGGCAGCGGCTACAAGGGACGCTGCGGCATCTACCAGGTTATGCCGATCA
CCGAGGCGATGCAGCAGATCATCCTGTCGCACGGTACGGCGCTGCAGATTGCCGAGCAGGCGCGGCGCGACGGCGTGCTA
TCGTTGCGGGAAGCTGGCCTGCTCAAGGTCAAGCAAGGGGTCACGTCACTCGAAGAAGTGCTGGCCACCACCAATTCATA
A

Upstream 100 bases:

>100_bases
AATGCCGACACCGTCCATTTGTCGCCTTTACAAAAAGTGACCCCGGCGGCAAACTGCGCCGATCCGGGGCCCATGGCCCC
GACCGGCGCCTCCGGGCGCA

Downstream 100 bases:

>100_bases
AACTACAGACAAAACATTCATGTCGGGGGTCGATCATGGCAACGCGCGCACCAGCTGCGAGCGCACGGGCGGCAGTGCCG
CCACGCGCGAAGGCAGGACG

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MHYLVPAMTLGLALAQSRRIAPALLAQLEQSAREKQTQLIDEIVGTGTMTAHDVALFAADKYQLPLLDLSQYNMNKVPSA
LAGNREFHAHRLLPLGRRENRLVVAMSDPSNQAGLDAIKQKYNLPLDTVVVEHDKLMKHVRSAGEALGTLKDVAPGTPTE
RKMIEYDPVAAAAQRPRTGATNDIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLHEVARPPLDIRDK
IATRIKVLSRLDISEKRVPQDGRMKLLIALPKDKDKDGKDKETVEKAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQL
GYEPEQKALLLETIKRPYGMILVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAAL
RSFLRQDPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCTC
KKPGTIPREALLDAGFKEPQLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQARRDGVL
SLREAGLLKVKQGVTSLEEVLATTNS

Sequences:

>Translated_586_residues
MHYLVPAMTLGLALAQSRRIAPALLAQLEQSAREKQTQLIDEIVGTGTMTAHDVALFAADKYQLPLLDLSQYNMNKVPSA
LAGNREFHAHRLLPLGRRENRLVVAMSDPSNQAGLDAIKQKYNLPLDTVVVEHDKLMKHVRSAGEALGTLKDVAPGTPTE
RKMIEYDPVAAAAQRPRTGATNDIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLHEVARPPLDIRDK
IATRIKVLSRLDISEKRVPQDGRMKLLIALPKDKDKDGKDKETVEKAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQL
GYEPEQKALLLETIKRPYGMILVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAAL
RSFLRQDPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCTC
KKPGTIPREALLDAGFKEPQLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQARRDGVL
SLREAGLLKVKQGVTSLEEVLATTNS
>Mature_586_residues
MHYLVPAMTLGLALAQSRRIAPALLAQLEQSAREKQTQLIDEIVGTGTMTAHDVALFAADKYQLPLLDLSQYNMNKVPSA
LAGNREFHAHRLLPLGRRENRLVVAMSDPSNQAGLDAIKQKYNLPLDTVVVEHDKLMKHVRSAGEALGTLKDVAPGTPTE
RKMIEYDPVAAAAQRPRTGATNDIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLHEVARPPLDIRDK
IATRIKVLSRLDISEKRVPQDGRMKLLIALPKDKDKDGKDKETVEKAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQL
GYEPEQKALLLETIKRPYGMILVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAAL
RSFLRQDPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCTC
KKPGTIPREALLDAGFKEPQLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQARRDGVL
SLREAGLLKVKQGVTSLEEVLATTNS

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=399, Percent_Identity=44.3609022556391, Blast_Score=325, Evalue=6e-90,
Organism=Escherichia coli, GI1789723, Length=402, Percent_Identity=44.2786069651741, Blast_Score=318, Evalue=9e-88,
Organism=Escherichia coli, GI87082188, Length=269, Percent_Identity=31.9702602230483, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 64576; Mature: 64576

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYLVPAMTLGLALAQSRRIAPALLAQLEQSAREKQTQLIDEIVGTGTMTAHDVALFAAD
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECC
KYQLPLLDLSQYNMNKVPSALAGNREFHAHRLLPLGRRENRLVVAMSDPSNQAGLDAIKQ
CCCCCEECCCCCCHHHCCHHHCCCCCHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHH
KYNLPLDTVVVEHDKLMKHVRSAGEALGTLKDVAPGTPTERKMIEYDPVAAAAQRPRTGA
HHCCCHHHEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHCCCCCCC
TNDIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLHEVARPPLDIRDK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
IATRIKVLSRLDISEKRVPQDGRMKLLIALPKDKDKDGKDKETVEKAVDFRVSTLPTLFG
HHHHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EKIVMRILESSSDKLDIDQLGYEPEQKALLLETIKRPYGMILVTGPTGSGKTVSLYTFLN
HHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHH
LLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALRSFLRQDPDIIMVGEIRDLE
HHCCCCCEEECCCCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCC
TADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCTC
CCCEEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
KKPGTIPREALLDAGFKEPQLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQI
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHH
ILSHGTALQIAEQARRDGVLSLREAGLLKVKQGVTSLEEVLATTNS
HHHCCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHYLVPAMTLGLALAQSRRIAPALLAQLEQSAREKQTQLIDEIVGTGTMTAHDVALFAAD
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECC
KYQLPLLDLSQYNMNKVPSALAGNREFHAHRLLPLGRRENRLVVAMSDPSNQAGLDAIKQ
CCCCCEECCCCCCHHHCCHHHCCCCCHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHH
KYNLPLDTVVVEHDKLMKHVRSAGEALGTLKDVAPGTPTERKMIEYDPVAAAAQRPRTGA
HHCCCHHHEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHCCCCCCC
TNDIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLHEVARPPLDIRDK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
IATRIKVLSRLDISEKRVPQDGRMKLLIALPKDKDKDGKDKETVEKAVDFRVSTLPTLFG
HHHHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EKIVMRILESSSDKLDIDQLGYEPEQKALLLETIKRPYGMILVTGPTGSGKTVSLYTFLN
HHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHH
LLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALRSFLRQDPDIIMVGEIRDLE
HHCCCCCEEECCCCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCC
TADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCTC
CCCEEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
KKPGTIPREALLDAGFKEPQLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQI
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHH
ILSHGTALQIAEQARRDGVLSLREAGLLKVKQGVTSLEEVLATTNS
HHHCCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]