Click here to switch to the map view.

The map label for this gene is tilS/hprT [H]

Identifier: 73542632

GI number: 73542632

Start: 3234309

End: 3234851

Strand: Direct

Name: tilS/hprT [H]

Synonym: Reut_A2948

Alternate gene names: 73542632

Gene position: 3234309-3234851 (Clockwise)

Preceding gene: 73542631

Following gene: 73542637

Centisome position: 84.97

GC content: 61.88

Gene sequence:

>543_bases
ATGATGACCGCTGAACAGGCCCGCGAACTGTGGGCCAACTCCGAGGAAATCGTCAGCGCCCAGGAAGTCCAGGCATCGCT
GGACCGCATGGCGAAAGACATCACCGCGAAGATGGGCGATGCCTTCCCGCTGGTGCTGTCTGTGATGGGCGGCGCGGTGG
TGTTCACCGGCATGCTGCTGCCCAAGCTGCAGTTCCCGCTGGAATTCGACTACATCCACCTGTCCCGCTACAACAACAAG
ACCGTGGGCGGCGAGATGCAATGGCGCGTGGCGCCGCGTGAATCCGTCAGGGATCGCGTGGTGCTGGTGCTCGACGATAT
CCTCGACGAAGGCGAGACCATGGCGGCCATCCGCCAGCGCATCATGGATATGGGCGCCAAGGAATTCCACGCCGCGGTGC
TGTGCGAGAAAACGCTGACCAAGCTCAAGCCGATGCATCCGGATTTCTGCGGCTTCCAGGTGCCGGACCGGTATGTGTTT
GGTTGCGGGATGGACGCGAAGGGATACTGGCGGAATCTGGGGACGATTCGGGCGCTGATGTAA

Upstream 100 bases:

>100_bases
GGTGCAATGAAGGGCGGCATGAAGGGCCTTTTCAACCGCTGATCGGCGCCGCGCTCCCTACCTGCCCTACCCCGCAAGAG
ACAAGAACAGGAAAGACATC

Downstream 100 bases:

>100_bases
GCGCCCGTCAGCCGGCGTGGTATGCCCCCAGAATCGGCCACAGCGACGCCAGCAGCAGCAACGCCATCCCCACATTGAAC
ACGCGCAGCACGCGCGGCTG

Product: hypoxanthine-guanine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MMTAEQARELWANSEEIVSAQEVQASLDRMAKDITAKMGDAFPLVLSVMGGAVVFTGMLLPKLQFPLEFDYIHLSRYNNK
TVGGEMQWRVAPRESVRDRVVLVLDDILDEGETMAAIRQRIMDMGAKEFHAAVLCEKTLTKLKPMHPDFCGFQVPDRYVF
GCGMDAKGYWRNLGTIRALM

Sequences:

>Translated_180_residues
MMTAEQARELWANSEEIVSAQEVQASLDRMAKDITAKMGDAFPLVLSVMGGAVVFTGMLLPKLQFPLEFDYIHLSRYNNK
TVGGEMQWRVAPRESVRDRVVLVLDDILDEGETMAAIRQRIMDMGAKEFHAAVLCEKTLTKLKPMHPDFCGFQVPDRYVF
GCGMDAKGYWRNLGTIRALM
>Mature_180_residues
MMTAEQARELWANSEEIVSAQEVQASLDRMAKDITAKMGDAFPLVLSVMGGAVVFTGMLLPKLQFPLEFDYIHLSRYNNK
TVGGEMQWRVAPRESVRDRVVLVLDDILDEGETMAAIRQRIMDMGAKEFHAAVLCEKTLTKLKPMHPDFCGFQVPDRYVF
GCGMDAKGYWRNLGTIRALM

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=159, Percent_Identity=27.0440251572327, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI9910262, Length=160, Percent_Identity=28.125, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI87081689, Length=165, Percent_Identity=23.6363636363636, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17510817, Length=162, Percent_Identity=28.3950617283951, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20309; Mature: 20309

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
7.2 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
7.2 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTAEQARELWANSEEIVSAQEVQASLDRMAKDITAKMGDAFPLVLSVMGGAVVFTGMLL
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
PKLQFPLEFDYIHLSRYNNKTVGGEMQWRVAPRESVRDRVVLVLDDILDEGETMAAIRQR
HHHCCCEEECEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IMDMGAKEFHAAVLCEKTLTKLKPMHPDFCGFQVPDRYVFGCGMDAKGYWRNLGTIRALM
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MMTAEQARELWANSEEIVSAQEVQASLDRMAKDITAKMGDAFPLVLSVMGGAVVFTGMLL
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
PKLQFPLEFDYIHLSRYNNKTVGGEMQWRVAPRESVRDRVVLVLDDILDEGETMAAIRQR
HHHCCCEEECEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IMDMGAKEFHAAVLCEKTLTKLKPMHPDFCGFQVPDRYVFGCGMDAKGYWRNLGTIRALM
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]