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The map label for this gene is pilT [H]

Identifier: 73542484

GI number: 73542484

Start: 3068047

End: 3069186

Strand: Reverse

Name: pilT [H]

Synonym: Reut_A2799

Alternate gene names: 73542484

Gene position: 3069186-3068047 (Counterclockwise)

Preceding gene: 73542485

Following gene: 73542483

Centisome position: 80.63

GC content: 61.58

Gene sequence:

>1140_bases
ATGCTCGACCGCGAATCCGCCGCCAAGTACATCAACGACCTGCTCGAGCTTATGGTGAGCAACCGCGGGTCGGACCTGTT
CATCACCTCCGACTTTCCGCCGGCTATCAAGGTTGATGGCAAGATCACGCCCGTCTCGCAGCAGCCGCTGAATCCTACGC
AGGCGCTGGGCCTGGTGCGTTCGATCATGAACGAGCGCCAGGTCAAGGAGTTCGACGACAGCCGTGAATGCAACTTCGCC
ATCTCGGCGCCCAAGGCCGGCCGCTTCCGCGTATCGGCCTTTATCCAGCAGGGCAAGGCCGGCATGGTGGTGCGGACCAT
CAACACGCGCATTCCGTCGGTGTCGGACCTTGACTTGCCGCCCACGCTTCACGAGATCGTCATGTCCAAGCGCGGCCTGG
TGATCGTGACCGGCGCCACGGGCTCGGGCAAGTCGACCACGCTCGCGGCGATGCTCGATCACCGCAATGCGCATTCGTAC
GGTCACATCATCACCATCGAGGACCCGATCGAATACGTGCATGCGCACCAGAACTGCGTCGTCACACAGCGCGAGGTGGG
CATCGATACCGAGTCCTGGCATGTGGCGCTGAAGAACACCCTGCGCCAGGCGCCCGATGTGATCCTGATCGGCGAAATCC
GCGATCGCGAGACCATGGAGTACGCGATGCAGTACGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCCAACCAGGCGATCGACCGTATCGTCAACTTCTTCCCGGAAGAGAAGCGCCAGCAGCTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCCATGGTGTCGCAGCGGCTGCTGCCGCGAGCGGGCCGCAAGGGGCGTGTGCCGGCCGTCGAAATCATGATCG
GCACGCCGCTGGTGGCCGACCTTATCTTCAAGGGCGAGATCCACGAACTCAAGGAAGTGATCAAGAAATCGCGCGAGCAG
GGCATGATTTCGTTCGACCAGGCGTTGTTCGACCTGCACGAAAGCGGCAAGATCACGTATGAGGACGCGCTGCGCAATGC
CGATTCGCTCAATGACCTTCGGCTGATGATCAAGCTGCACAGTGCGACCAGGAAGGACAGCGATTTGGGTGCCGGCACCG
AGCACTTGAACGTTATCTGA

Upstream 100 bases:

>100_bases
GCCTTCATGGGCTGAGTCCCGCGCCGTGCCGTGCCGCGCCATGCGCGCGGCCGCCGTTCCGCTCCATCCGCCCAATTCGT
CCATTCAGCAGGACCACGCC

Downstream 100 bases:

>100_bases
TCCGCGCGTCCGTTCCAGGAGCCAAGTCCGCATGAGCAATGTCTACCAGTTCGAAGCCAAGTCCCTCGCGGGGCAGCCCG
TGCCGCTGTCGCAGTTCCGC

Product: pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKITPVSQQPLNPTQALGLVRSIMNERQVKEFDDSRECNFA
ISAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVSDLDLPPTLHEIVMSKRGLVIVTGATGSGKSTTLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCVVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFDLHESGKITYEDALRNADSLNDLRLMIKLHSATRKDSDLGAGTEHLNVI

Sequences:

>Translated_379_residues
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKITPVSQQPLNPTQALGLVRSIMNERQVKEFDDSRECNFA
ISAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVSDLDLPPTLHEIVMSKRGLVIVTGATGSGKSTTLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCVVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFDLHESGKITYEDALRNADSLNDLRLMIKLHSATRKDSDLGAGTEHLNVI
>Mature_379_residues
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKITPVSQQPLNPTQALGLVRSIMNERQVKEFDDSRECNFA
ISAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVSDLDLPPTLHEIVMSKRGLVIVTGATGSGKSTTLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCVVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFDLHESGKITYEDALRNADSLNDLRLMIKLHSATRKDSDLGAGTEHLNVI

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=318, Percent_Identity=38.3647798742138, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1789723, Length=157, Percent_Identity=40.7643312101911, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786296, Length=384, Percent_Identity=25.5208333333333, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42188; Mature: 42188

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKITPVSQQPLNPTQALGLVR
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCEEECCCCCCCCHHHHHHHHH
SIMNERQVKEFDDSRECNFAISAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVSDLDLP
HHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
PTLHEIVMSKRGLVIVTGATGSGKSTTLAAMLDHRNAHSYGHIITIEDPIEYVHAHQNCV
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHCCCEE
VTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
EEHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCC
ANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRAGRKGRVPAVEIMIGTPLVAD
HHHHHHHHHHHCCCCCHHEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHH
LIFKGEIHELKEVIKKSREQGMISFDQALFDLHESGKITYEDALRNADSLNDLRLMIKLH
HHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEHHHHHCCCCCHHHHEEEEEEH
SATRKDSDLGAGTEHLNVI
HHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKITPVSQQPLNPTQALGLVR
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCEEECCCCCCCCHHHHHHHHH
SIMNERQVKEFDDSRECNFAISAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVSDLDLP
HHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCC
PTLHEIVMSKRGLVIVTGATGSGKSTTLAAMLDHRNAHSYGHIITIEDPIEYVHAHQNCV
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHCCCEE
VTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
EEHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCC
ANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRAGRKGRVPAVEIMIGTPLVAD
HHHHHHHHHHHCCCCCHHEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHH
LIFKGEIHELKEVIKKSREQGMISFDQALFDLHESGKITYEDALRNADSLNDLRLMIKLH
HHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEHHHHHCCCCCHHHHEEEEEEH
SATRKDSDLGAGTEHLNVI
HHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]