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The map label for this gene is pilT [H]

Identifier: 73542485

GI number: 73542485

Start: 3069272

End: 3070315

Strand: Reverse

Name: pilT [H]

Synonym: Reut_A2800

Alternate gene names: 73542485

Gene position: 3070315-3069272 (Counterclockwise)

Preceding gene: 73542488

Following gene: 73542484

Centisome position: 80.66

GC content: 62.16

Gene sequence:

>1044_bases
ATGGACATCGCGCAGCTATTGGCTTTCGCAGTCAAGAACAAGGCGTCCGATTTGCATCTCTCGGCGGACATGCCGCCGAT
GGTACGTATCCACGGCGACATGCGGCGCATCAATGTCGCGTCCATGGCGCACAAGGACGTCCACGCCATGGTGTACGACA
TCATGAGCGACTCCCAGCGCAAGGTTTACGAAGAACGGCTCGAGATCGATTTCTCGTTCGAGATCGGCGGCCTGTCGCGT
TTCCGGGTCAACGCCTACAACACGCAACGCGGCGCGGCTGCGGTGTTCCGTACGATTCCCTCGAAGGTTCTCACGCTTGA
AGAGCTGCGCGCGCCGGCGGTGTTCGCCGACCTGTGCATGAAGCCGCGTGGCCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCGGCAATGGTCGATCACCGCAACGAAAACGACATGGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACCAGTCGAAAAAGAGCCTGATCAACCAGCGCGAACTCGGTCCGCACACGCATTCGTTTGCCAA
CGCGCTGCGTTCGGCACTGCGTGAAGACCCGGACGTGATCCTCGTCGGCGAATTGCGCGATCTTGAAACCATCCGCCTCG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTTTTCGGCACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGTGTG
GTAGACGTGTTCCCGCCCGAAGAGAAGGACATGGTGCGCACAATGCTGTCCGAATCGCTCGAGGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCAATGGCCGTACCGCGGCGCACGAGATCATGATCGCCACGCCCGCGATCCGCCATCTGA
TCCGCGAGAACAAGATTGCACAGATGTACTCGATGATGCAGACCAGCAGCGGCCTGGGCATGCAGACGCTGGACCAGTGC
CTGTCGGACCTGATCAAGCGCAGCCTGATCAGCTACAGCGACGCGCGCGCCATCGCCAAGAACCCGGACGCCTTCATGGG
CTGA

Upstream 100 bases:

>100_bases
AGTTGGCGGCAATTACAGACATAATCCGGCGGACATACAGAAGAAGTAAAAAGAAGTACTAAAAGTACTGCATACAATTA
ATCGGGTGGGGTCATTATAG

Downstream 100 bases:

>100_bases
GTCCCGCGCCGTGCCGTGCCGCGCCATGCGCGCGGCCGCCGTTCCGCTCCATCCGCCCAATTCGTCCATTCAGCAGGACC
ACGCCATGCTCGACCGCGAA

Product: pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVASMAHKDVHAMVYDIMSDSQRKVYEERLEIDFSFEIGGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNENDMGHILTVED
PIEFVHQSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRSLISYSDARAIAKNPDAFMG

Sequences:

>Translated_347_residues
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVASMAHKDVHAMVYDIMSDSQRKVYEERLEIDFSFEIGGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNENDMGHILTVED
PIEFVHQSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRSLISYSDARAIAKNPDAFMG
>Mature_347_residues
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVASMAHKDVHAMVYDIMSDSQRKVYEERLEIDFSFEIGGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNENDMGHILTVED
PIEFVHQSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRSLISYSDARAIAKNPDAFMG

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=319, Percent_Identity=47.6489028213166, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1786296, Length=378, Percent_Identity=30.4232804232804, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789723, Length=349, Percent_Identity=33.5243553008596, Blast_Score=130, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38393; Mature: 38393

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVASMAHKDVHAMVYDIMSDSQR
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
KVYEERLEIDFSFEIGGLSRFRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCM
HHHHHHHCEEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
KPRGLVLVTGPTGSGKSTTLAAMVDHRNENDMGHILTVEDPIEFVHQSKKSLINQRELGP
CCCCEEEEECCCCCCCCCEEEEEHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCC
HTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
CHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHEEEEECCCCCEEEEEEECCHHHHHHHHH
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEECHHHHHHHHHHHHH
QMYSMMQTSSGLGMQTLDQCLSDLIKRSLISYSDARAIAKNPDAFMG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVASMAHKDVHAMVYDIMSDSQR
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHH
KVYEERLEIDFSFEIGGLSRFRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCM
HHHHHHHCEEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
KPRGLVLVTGPTGSGKSTTLAAMVDHRNENDMGHILTVEDPIEFVHQSKKSLINQRELGP
CCCCEEEEECCCCCCCCCEEEEEHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCC
HTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
CHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHEEEEECCCCCEEEEEEECCHHHHHHHHH
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEECHHHHHHHHHHHHH
QMYSMMQTSSGLGMQTLDQCLSDLIKRSLISYSDARAIAKNPDAFMG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]