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The map label for this gene is braC [H]

Identifier: 73542414

GI number: 73542414

Start: 2995715

End: 2996917

Strand: Reverse

Name: braC [H]

Synonym: Reut_A2729

Alternate gene names: 73542414

Gene position: 2996917-2995715 (Counterclockwise)

Preceding gene: 73542415

Following gene: 73542413

Centisome position: 78.73

GC content: 62.84

Gene sequence:

>1203_bases
ATGCAAATCACGTTTGCCAAGATTCTGCCGATCGCGGCCGCAGTGGCGCTCGTTACAGCTTGCGGCAAGAAAGAAGAAAA
GCCGGCTGAACCGTCGGCGTCGGCTCCGGCAGCGGCTGCGCCGGCCGCAGGCGGCGGTGAAGTCGTCGTCAAGATCGGCC
ACGCGGCTCCGCTGACCGGCGGTATCGCCCACCTTGGCAAGGACAACGAAAACGGCGCCCGCCTGGCCGTGGAAGAAGTC
AACAAGAACGGTCTGGAGATCGGCGGCAAGAAGGTCAAGCTGGAACTGATCGGCGAAGACGACGCCGCGGATCCGAAGAC
CGGCACCGCCGTTGCCCAGAAGCTGGTCGACCAGAAGGTCGTGGCCGTGGTCGGCCACCTGAACTCGGGCGTGTCGATCC
CGGCCTCGAAGATCTACAGCGATGCCGGCATTGTCCAGATCTCGCCGTCGTCGACCAACCCGGACTACACCAAGCAAGGC
TTCAAGACGACCTACCGCGTGGTCGCCACCGATGCCCAGCAAGGTCCGGCACTGGCCAACTACGCCGCCAAGACCCTGCA
TGCCAAGAGCGTGGCGATCGTCGACGACGCTACCGCCTACGGCAAGGGCCTGGCCGACGAGTTCGAGAAGACCGCGAAGG
CGGCCGGCGTGAACGTGGTGGCGCGTGAAGCCACCAACGACAAGGCCACCGACTTCAAGGCCATTCTGACCAAGATCAAG
GGCAAGAAGCCGGACGTCATCATGTACGGCGGCATGGACGCCACCGGCGGCCCGTTCGCCAAGCAGGCCAAGGAACTGGG
CATCTCGTCCAAGATCGTTGGCGGCGACGGCGTCTGTACCGACAAGGTGGCCGAGCTGGCCGGCGATGCCGTGCAGAACA
TCATCTGCTCCGAGGCAGGCCTGGCCCTGTCGAAGATGGAGAACGGCGCCGACTTCGAGAAGCGCTACCAGGCTCGTTTC
AACGCGCCGGTGCAGATCTATGCACCGTTCACGTATGACGCCGTGATGGTCATCGTCGACGCCATGAAGCGCGCCAACTC
GACCGATCCGGCAGCCATCCTGGCCGAAATGCCCAAGACCAACTACAAGGGCGTGATCGGCAACATCGCCTTCGACGAGA
AGGGCGACATGAAGGAAGGCACCATCACGCTGTACGAGTACAAGGACAAGAAGAAGTCCGTTCTCGACGTCGTGAAGATG
TAA

Upstream 100 bases:

>100_bases
ACAATGCGCGCGTTTCAAATTGAAACTAAACAAAGGCGGTATGGGGACGCGCCCGGGAGGCGTTGCAAATCCCCATTTTG
TGAATCCTAGGAGATCACTC

Downstream 100 bases:

>100_bases
TTCGGGCTTACCGGCCCATCCGAACGGCACCGTGAGTATTTTGCGGTGCCGTTCTTTTTAGCCTCGCCTAATGTAGAAGC
ATCCTTACCATGGATGGAGC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MQITFAKILPIAAAVALVTACGKKEEKPAEPSASAPAAAAPAAGGGEVVVKIGHAAPLTGGIAHLGKDNENGARLAVEEV
NKNGLEIGGKKVKLELIGEDDAADPKTGTAVAQKLVDQKVVAVVGHLNSGVSIPASKIYSDAGIVQISPSSTNPDYTKQG
FKTTYRVVATDAQQGPALANYAAKTLHAKSVAIVDDATAYGKGLADEFEKTAKAAGVNVVAREATNDKATDFKAILTKIK
GKKPDVIMYGGMDATGGPFAKQAKELGISSKIVGGDGVCTDKVAELAGDAVQNIICSEAGLALSKMENGADFEKRYQARF
NAPVQIYAPFTYDAVMVIVDAMKRANSTDPAAILAEMPKTNYKGVIGNIAFDEKGDMKEGTITLYEYKDKKKSVLDVVKM

Sequences:

>Translated_400_residues
MQITFAKILPIAAAVALVTACGKKEEKPAEPSASAPAAAAPAAGGGEVVVKIGHAAPLTGGIAHLGKDNENGARLAVEEV
NKNGLEIGGKKVKLELIGEDDAADPKTGTAVAQKLVDQKVVAVVGHLNSGVSIPASKIYSDAGIVQISPSSTNPDYTKQG
FKTTYRVVATDAQQGPALANYAAKTLHAKSVAIVDDATAYGKGLADEFEKTAKAAGVNVVAREATNDKATDFKAILTKIK
GKKPDVIMYGGMDATGGPFAKQAKELGISSKIVGGDGVCTDKVAELAGDAVQNIICSEAGLALSKMENGADFEKRYQARF
NAPVQIYAPFTYDAVMVIVDAMKRANSTDPAAILAEMPKTNYKGVIGNIAFDEKGDMKEGTITLYEYKDKKKSVLDVVKM
>Mature_400_residues
MQITFAKILPIAAAVALVTACGKKEEKPAEPSASAPAAAAPAAGGGEVVVKIGHAAPLTGGIAHLGKDNENGARLAVEEV
NKNGLEIGGKKVKLELIGEDDAADPKTGTAVAQKLVDQKVVAVVGHLNSGVSIPASKIYSDAGIVQISPSSTNPDYTKQG
FKTTYRVVATDAQQGPALANYAAKTLHAKSVAIVDDATAYGKGLADEFEKTAKAAGVNVVAREATNDKATDFKAILTKIK
GKKPDVIMYGGMDATGGPFAKQAKELGISSKIVGGDGVCTDKVAELAGDAVQNIICSEAGLALSKMENGADFEKRYQARF
NAPVQIYAPFTYDAVMVIVDAMKRANSTDPAAILAEMPKTNYKGVIGNIAFDEKGDMKEGTITLYEYKDKKKSVLDVVKM

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=345, Percent_Identity=32.7536231884058, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI48994941, Length=338, Percent_Identity=28.9940828402367, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41603; Mature: 41603

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITFAKILPIAAAVALVTACGKKEEKPAEPSASAPAAAAPAAGGGEVVVKIGHAAPLTG
CEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
GIAHLGKDNENGARLAVEEVNKNGLEIGGKKVKLELIGEDDAADPKTGTAVAQKLVDQKV
CHHHCCCCCCCCCEEEEEHHCCCCCEECCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
VAVVGHLNSGVSIPASKIYSDAGIVQISPSSTNPDYTKQGFKTTYRVVATDAQQGPALAN
HHHHHHCCCCCCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHEEEEEEEECCCCCCHHHH
YAAKTLHAKSVAIVDDATAYGKGLADEFEKTAKAAGVNVVAREATNDKATDFKAILTKIK
HHHHHHHCCEEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHC
GKKPDVIMYGGMDATGGPFAKQAKELGISSKIVGGDGVCTDKVAELAGDAVQNIICSEAG
CCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
LALSKMENGADFEKRYQARFNAPVQIYAPFTYDAVMVIVDAMKRANSTDPAAILAEMPKT
CHHHHHCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
NYKGVIGNIAFDEKGDMKEGTITLYEYKDKKKSVLDVVKM
CCCEEEEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHCC
>Mature Secondary Structure
MQITFAKILPIAAAVALVTACGKKEEKPAEPSASAPAAAAPAAGGGEVVVKIGHAAPLTG
CEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
GIAHLGKDNENGARLAVEEVNKNGLEIGGKKVKLELIGEDDAADPKTGTAVAQKLVDQKV
CHHHCCCCCCCCCEEEEEHHCCCCCEECCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
VAVVGHLNSGVSIPASKIYSDAGIVQISPSSTNPDYTKQGFKTTYRVVATDAQQGPALAN
HHHHHHCCCCCCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHEEEEEEEECCCCCCHHHH
YAAKTLHAKSVAIVDDATAYGKGLADEFEKTAKAAGVNVVAREATNDKATDFKAILTKIK
HHHHHHHCCEEEEEECCHHHCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHC
GKKPDVIMYGGMDATGGPFAKQAKELGISSKIVGGDGVCTDKVAELAGDAVQNIICSEAG
CCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
LALSKMENGADFEKRYQARFNAPVQIYAPFTYDAVMVIVDAMKRANSTDPAAILAEMPKT
CHHHHHCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
NYKGVIGNIAFDEKGDMKEGTITLYEYKDKKKSVLDVVKM
CCCEEEEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]