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The map label for this gene is ispH [H]

Identifier: 73542415

GI number: 73542415

Start: 2997538

End: 2998512

Strand: Reverse

Name: ispH [H]

Synonym: Reut_A2730

Alternate gene names: 73542415

Gene position: 2998512-2997538 (Counterclockwise)

Preceding gene: 73542416

Following gene: 73542414

Centisome position: 78.77

GC content: 66.97

Gene sequence:

>975_bases
ATGCCTGACCTGACTACCGCACCCGATGCCGAGATCCTGATGGCGCAGCCGCGCGGCTTTTGCGCCGGCGTCGACCGCGC
CATCGAGATCGTCGAGCGCGCGCTTACGCGTTTCGGGGCGCCCATCTATGTGCGTCACGAGATTGTCCACAACGCCTATG
TCGTCGCCGACCTGCGCCGCAAGGGCGCGGTGTTCGTGCGCGAGCTCGATGAAGTGCCGGCTGGCGCTACTGTCATCTTC
AGCGCCCACGGCGTGTCGAAGGACGTTCGCGCCGAAGCTGCCTCACGCGGCCTCAACGTGTTCGACGCGACTTGCCCGCT
CGTGACCAAGGTCCATGTCGAAGTCGGCAAGATGCGCGCGGAGGGCTGCGAGATCGTGATGATCGGCCACCGCGGGCACC
CGGAAGTCGAAGGCACCATGGGCCAGGCCGAAGGCGGCATGCTGCTGGTGGAGTCGGTGGCGGATGTCGCAGGGCTGGTG
GTTGCCGATCCCGCGCGCCTGGCGTACGTGACGCAGACCACGCTGTCGGTCGATGAAACCCTCGAGATCGTGGCCGCGCT
CAAGGCCCGTTTCCCGCTGATCCGCGAGCCAAAGAAACAGGACATCTGCTACGCCACGCAGAACCGCCAGGATGCCGTTA
AGTTCATGGCGCCCCAGGTCGATGTGGTGATCGTGGTGGGCAGCCCCAACAGTTCGAACTCGAACCGGTTGCGCGAACTG
GCCGCACGACTGGGCGTGCCCGCCTATATGGTGGATGCACCCGATCAGGTGCACCCGGAATGGGTGGCGGGCAAGCGGCG
CATCGGCCTGACCGCCGGCGCATCGGCGCCCGAGGAACTGGCCCAGTCCATCGTTGCACGACTGCGCGAGCTCGGTGCCG
GCCACGTGCGTGCCCTTGCCGGCATCGAAGAAAACATGGCGTTCCCGCTACCGCGCGGCCTCCAGGCCGCTGCCACGGTC
GGCGTCACGGCCTGA

Upstream 100 bases:

>100_bases
CTGTTCGATTTCAACCATCCGCTGGCCGGGCAGACGATCCTGTTCGACGTGCAGCTGATCGGGATCCTCTGATTGCGCTC
ACTTGTCCTGACATTGCAGC

Downstream 100 bases:

>100_bases
CCTGCTGACCTGTCTTGCCGGCGCCGCGTTCTGCGGCGCCTCTCTCCTGCCTTTTCGTCAGACTGCTTTCGCGCGATGCG
TGCGTGATCGCGCGCGCATT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MPDLTTAPDAEILMAQPRGFCAGVDRAIEIVERALTRFGAPIYVRHEIVHNAYVVADLRRKGAVFVRELDEVPAGATVIF
SAHGVSKDVRAEAASRGLNVFDATCPLVTKVHVEVGKMRAEGCEIVMIGHRGHPEVEGTMGQAEGGMLLVESVADVAGLV
VADPARLAYVTQTTLSVDETLEIVAALKARFPLIREPKKQDICYATQNRQDAVKFMAPQVDVVIVVGSPNSSNSNRLREL
AARLGVPAYMVDAPDQVHPEWVAGKRRIGLTAGASAPEELAQSIVARLRELGAGHVRALAGIEENMAFPLPRGLQAAATV
GVTA

Sequences:

>Translated_324_residues
MPDLTTAPDAEILMAQPRGFCAGVDRAIEIVERALTRFGAPIYVRHEIVHNAYVVADLRRKGAVFVRELDEVPAGATVIF
SAHGVSKDVRAEAASRGLNVFDATCPLVTKVHVEVGKMRAEGCEIVMIGHRGHPEVEGTMGQAEGGMLLVESVADVAGLV
VADPARLAYVTQTTLSVDETLEIVAALKARFPLIREPKKQDICYATQNRQDAVKFMAPQVDVVIVVGSPNSSNSNRLREL
AARLGVPAYMVDAPDQVHPEWVAGKRRIGLTAGASAPEELAQSIVARLRELGAGHVRALAGIEENMAFPLPRGLQAAATV
GVTA
>Mature_323_residues
PDLTTAPDAEILMAQPRGFCAGVDRAIEIVERALTRFGAPIYVRHEIVHNAYVVADLRRKGAVFVRELDEVPAGATVIFS
AHGVSKDVRAEAASRGLNVFDATCPLVTKVHVEVGKMRAEGCEIVMIGHRGHPEVEGTMGQAEGGMLLVESVADVAGLVV
ADPARLAYVTQTTLSVDETLEIVAALKARFPLIREPKKQDICYATQNRQDAVKFMAPQVDVVIVVGSPNSSNSNRLRELA
ARLGVPAYMVDAPDQVHPEWVAGKRRIGLTAGASAPEELAQSIVARLRELGAGHVRALAGIEENMAFPLPRGLQAAATVG
VTA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=308, Percent_Identity=59.4155844155844, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34560; Mature: 34429

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLTTAPDAEILMAQPRGFCAGVDRAIEIVERALTRFGAPIYVRHEIVHNAYVVADLRR
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHEEEEHHH
KGAVFVRELDEVPAGATVIFSAHGVSKDVRAEAASRGLNVFDATCPLVTKVHVEVGKMRA
CCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCHHEEEHHHHHHHHC
EGCEIVMIGHRGHPEVEGTMGQAEGGMLLVESVADVAGLVVADPARLAYVTQTTLSVDET
CCCCEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHCEEEECCCCEEEEEEHHHCHHHH
LEIVAALKARFPLIREPKKQDICYATQNRQDAVKFMAPQVDVVIVVGSPNSSNSNRLREL
HHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHH
AARLGVPAYMVDAPDQVHPEWVAGKRRIGLTAGASAPEELAQSIVARLRELGAGHVRALA
HHHHCCCEEEECCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHH
GIEENMAFPLPRGLQAAATVGVTA
CCHHCCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure 
PDLTTAPDAEILMAQPRGFCAGVDRAIEIVERALTRFGAPIYVRHEIVHNAYVVADLRR
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHEEEEHHH
KGAVFVRELDEVPAGATVIFSAHGVSKDVRAEAASRGLNVFDATCPLVTKVHVEVGKMRA
CCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCHHEEEHHHHHHHHC
EGCEIVMIGHRGHPEVEGTMGQAEGGMLLVESVADVAGLVVADPARLAYVTQTTLSVDET
CCCCEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHHCEEEECCCCEEEEEEHHHCHHHH
LEIVAALKARFPLIREPKKQDICYATQNRQDAVKFMAPQVDVVIVVGSPNSSNSNRLREL
HHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHH
AARLGVPAYMVDAPDQVHPEWVAGKRRIGLTAGASAPEELAQSIVARLRELGAGHVRALA
HHHHCCCEEEECCCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHH
GIEENMAFPLPRGLQAAATVGVTA
CCHHCCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]