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The map label for this gene is rhtB [H]

Identifier: 73542038

GI number: 73542038

Start: 2591750

End: 2592397

Strand: Reverse

Name: rhtB [H]

Synonym: Reut_A2352

Alternate gene names: 73542038

Gene position: 2592397-2591750 (Counterclockwise)

Preceding gene: 73542040

Following gene: 73542029

Centisome position: 68.1

GC content: 69.91

Gene sequence:

>648_bases
ATGCCCGAATTCCTTCCTCCCTGGCCACTGCTCGGCGCATTCGTGCTGGCCAGCCTTGCGCTGGCGGTAACGCCGGGACC
CGCCGTGGTCTATATCGTGACGCGCACGCTCGCGCAGGGACGTCGCGCGGGGCTGGCGTCGGTCGGCGCCGTAGCGCTGG
GCAACCTGGGTAACGCGGCCGGCGCGGCGCTCGGGCTGGCGGTCTTGTTTTCGGTCTCGGCGCTGGCCTTTACGGTGGTG
AAGTACGCGGGGGCCGCGTACCTGATCTGGCTTGGCATCCGTACCTTGCGTGCAGCGCCGGCGGTCGCCACGGACACGCC
GCCGCCGGCACGGCCATTGCGCCAGGTGCTCCGCGATGGCTTCGTGGTAGCCTTGCTCAATCCCAAGACGGCCATTTTCT
TTGCCGCTTTCCTGCCGCAGTTCATGAACCCGGCCGGCTCCGCGCTGGCGCAGAGCCTTGCGCTGGGCATCGGCTTCGTG
CTGATCGCGGCCAGTACCGACATCTGCTACGTGACCGCCGCAGCTGCCGTCATGCCGGCACTGCGCCGCGCGGGTCGCGC
GCGTGCGGCGGGCCGCTACCTGACGGCCGCTGCCTTCATCGGACTGGGTGTGTTCACGGCGGCGTCGGGATCGCGCGCCG
CTAAGTGA

Upstream 100 bases:

>100_bases
GAGCCAATGCGCATAATGTTGCTTGGCAACCGTGTACGCGCTGCGTAGTCTGTTTCCCGCATTACCGCGAAAACGACAAA
CAAAGAAGCAGGAGACTCCC

Downstream 100 bases:

>100_bases
GGCAGGCTTGCCGCCGCGTCGTCAACGCAGCGGCAGTTCCAGGATGCCGCCCGCCGCAGGCCGGGCGCGCATGGCAGCCA
GCCAGCGCTCCAGGTCAGGA

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MPEFLPPWPLLGAFVLASLALAVTPGPAVVYIVTRTLAQGRRAGLASVGAVALGNLGNAAGAALGLAVLFSVSALAFTVV
KYAGAAYLIWLGIRTLRAAPAVATDTPPPARPLRQVLRDGFVVALLNPKTAIFFAAFLPQFMNPAGSALAQSLALGIGFV
LIAASTDICYVTAAAAVMPALRRAGRARAAGRYLTAAAFIGLGVFTAASGSRAAK

Sequences:

>Translated_215_residues
MPEFLPPWPLLGAFVLASLALAVTPGPAVVYIVTRTLAQGRRAGLASVGAVALGNLGNAAGAALGLAVLFSVSALAFTVV
KYAGAAYLIWLGIRTLRAAPAVATDTPPPARPLRQVLRDGFVVALLNPKTAIFFAAFLPQFMNPAGSALAQSLALGIGFV
LIAASTDICYVTAAAAVMPALRRAGRARAAGRYLTAAAFIGLGVFTAASGSRAAK
>Mature_214_residues
PEFLPPWPLLGAFVLASLALAVTPGPAVVYIVTRTLAQGRRAGLASVGAVALGNLGNAAGAALGLAVLFSVSALAFTVVK
YAGAAYLIWLGIRTLRAAPAVATDTPPPARPLRQVLRDGFVVALLNPKTAIFFAAFLPQFMNPAGSALAQSLALGIGFVL
IAASTDICYVTAAAAVMPALRRAGRARAAGRYLTAAAFIGLGVFTAASGSRAAK

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=180, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786522, Length=186, Percent_Identity=32.258064516129, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788099, Length=197, Percent_Identity=22.8426395939086, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21774; Mature: 21643

Theoretical pI: Translated: 11.98; Mature: 11.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEFLPPWPLLGAFVLASLALAVTPGPAVVYIVTRTLAQGRRAGLASVGAVALGNLGNAA
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHCCCCCHH
GAALGLAVLFSVSALAFTVVKYAGAAYLIWLGIRTLRAAPAVATDTPPPARPLRQVLRDG
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
FVVALLNPKTAIFFAAFLPQFMNPAGSALAQSLALGIGFVLIAASTDICYVTAAAAVMPA
EEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LRRAGRARAAGRYLTAAAFIGLGVFTAASGSRAAK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PEFLPPWPLLGAFVLASLALAVTPGPAVVYIVTRTLAQGRRAGLASVGAVALGNLGNAA
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHCCCCCHH
GAALGLAVLFSVSALAFTVVKYAGAAYLIWLGIRTLRAAPAVATDTPPPARPLRQVLRDG
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
FVVALLNPKTAIFFAAFLPQFMNPAGSALAQSLALGIGFVLIAASTDICYVTAAAAVMPA
EEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LRRAGRARAAGRYLTAAAFIGLGVFTAASGSRAAK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]