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The map label for this gene is prpC [H]

Identifier: 73542015

GI number: 73542015

Start: 2568103

End: 2569284

Strand: Direct

Name: prpC [H]

Synonym: Reut_A2329

Alternate gene names: 73542015

Gene position: 2568103-2569284 (Clockwise)

Preceding gene: 73542014

Following gene: 73542016

Centisome position: 67.47

GC content: 63.71

Gene sequence:

>1182_bases
ATGTCCGCAGCCAATCACACCACCCCGGCAGACGCTCCGCAATCGGCGGGACCGAAGCCCAAGAAGTCCGTTGCCCTTTC
GGGCGTGACCGCCGGTAATACCGCGCTATGTACCGTCGGCCGTACCGGCAACGACCTGCACTACCGCGGCTATGACATCC
TGGACGTGGCCGATGCATGCGAGTTCGAGGAAATCGCCCACCTGCTGGTCCATGGCAAGCTGCCGAACAGGGCCGAACTG
GCCGCCTACAAGACCAAGCTGAAGTCACTGCGCGGCCTGCCCGCCAACGTCAAGGCCGCGCTGGAATGGGTGCCTGCCTC
GGCCCACCCGATGGACGTGATGCGCACCGGCGTGTCGGTACTGGGCACCGTGCTGCCGGAGAAGGACGACCACAACGCCC
CGGGCGCTCGCGACATTGCCGACCGCCTGATGGCCAGCCTGGGCTCGATGCTGCTGTACTGGTACCACTACAGCCACAAC
GGTCGCCGCATCGAAGTGGAAACCGACGACGACTCGATCGGCGCCCACTTCCTGCATCTGCTGCATGGCGAAAAACCGTC
AAAGCTGTGGGAACGCGCCATGCACACCTCGATGATCCTGTACGCCGAGCACGAGTTCAACGCATCGACCTTCACCGGCC
GCGTGATCGCCGGCACGGGCTCGGACATGTACTCGGCCATCTGCGGCGCGATCGGCGCGCTGCGCGGCCCGAAGCACGGC
GGGGCCAATGAAGTGGCCTTCGAGATCCAGAAGCGCTACGACAGCCCGGACGAAGCGCAGGCCGACATCGCCCGCCGCGT
CGAGAACAAGGAAGTCGTGATCGGCTTCGGCCATCCGGTGTACACCATTGCCGACCCGCGCAACCAGGTGATCAAGGAGA
CCGCGCGCAACCTGTCGAAGGACGCCGGCTCGATGAAGATGTTCGACATCGCCGAACGCCTGGAAACGGTGATGTGGGAC
ATCAAGAAGATGTTCCCGAACCTGGACTGGTTCAGCGCCGTGAGCTACCACATGATGGGCGTGCCGACCGCAATGTTCAC
GCCGCTGTTCGTGATCGCTCGCACTTCGGGCTGGGCTGCGCATATCATCGAGCAGCGCATCGACAACAAGATCATCCGCC
CGAGCGCCAACTATACGGGCCCCGAAGATCTGAAGTTCGTACCGATCGGCAAGCGCAAGTGA

Upstream 100 bases:

>100_bases
TGCCGAAGAACGTGGCCGAGTTGTTCTTCGACTAGTCCGCGGCCCGCCCTGCACGCGCACAACAAGCATCCGGCATAGAC
CGATTCCAAAGGAGAACAGG

Downstream 100 bases:

>100_bases
TTGGCATGTGAGGCCCAGGCACCTGAGGCGCGGCATGTGAAGCGCGCCTCCGCCACATTCGCGCAGTATCGTCCGGATTA
TGTAACGAAAGGACAGGCTG

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSAANHTTPADAPQSAGPKPKKSVALSGVTAGNTALCTVGRTGNDLHYRGYDILDVADACEFEEIAHLLVHGKLPNRAEL
AAYKTKLKSLRGLPANVKAALEWVPASAHPMDVMRTGVSVLGTVLPEKDDHNAPGARDIADRLMASLGSMLLYWYHYSHN
GRRIEVETDDDSIGAHFLHLLHGEKPSKLWERAMHTSMILYAEHEFNASTFTGRVIAGTGSDMYSAICGAIGALRGPKHG
GANEVAFEIQKRYDSPDEAQADIARRVENKEVVIGFGHPVYTIADPRNQVIKETARNLSKDAGSMKMFDIAERLETVMWD
IKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRIDNKIIRPSANYTGPEDLKFVPIGKRK

Sequences:

>Translated_393_residues
MSAANHTTPADAPQSAGPKPKKSVALSGVTAGNTALCTVGRTGNDLHYRGYDILDVADACEFEEIAHLLVHGKLPNRAEL
AAYKTKLKSLRGLPANVKAALEWVPASAHPMDVMRTGVSVLGTVLPEKDDHNAPGARDIADRLMASLGSMLLYWYHYSHN
GRRIEVETDDDSIGAHFLHLLHGEKPSKLWERAMHTSMILYAEHEFNASTFTGRVIAGTGSDMYSAICGAIGALRGPKHG
GANEVAFEIQKRYDSPDEAQADIARRVENKEVVIGFGHPVYTIADPRNQVIKETARNLSKDAGSMKMFDIAERLETVMWD
IKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRIDNKIIRPSANYTGPEDLKFVPIGKRK
>Mature_392_residues
SAANHTTPADAPQSAGPKPKKSVALSGVTAGNTALCTVGRTGNDLHYRGYDILDVADACEFEEIAHLLVHGKLPNRAELA
AYKTKLKSLRGLPANVKAALEWVPASAHPMDVMRTGVSVLGTVLPEKDDHNAPGARDIADRLMASLGSMLLYWYHYSHNG
RRIEVETDDDSIGAHFLHLLHGEKPSKLWERAMHTSMILYAEHEFNASTFTGRVIAGTGSDMYSAICGAIGALRGPKHGG
ANEVAFEIQKRYDSPDEAQADIARRVENKEVVIGFGHPVYTIADPRNQVIKETARNLSKDAGSMKMFDIAERLETVMWDI
KKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAAHIIEQRIDNKIIRPSANYTGPEDLKFVPIGKRK

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=375, Percent_Identity=79.7333333333333, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI1786939, Length=363, Percent_Identity=31.9559228650138, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17555174, Length=361, Percent_Identity=24.6537396121884, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319850, Length=342, Percent_Identity=25.1461988304094, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324328, Length=342, Percent_Identity=24.5614035087719, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640126, Length=330, Percent_Identity=23.3333333333333, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640124, Length=330, Percent_Identity=23.3333333333333, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 43218; Mature: 43087

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAANHTTPADAPQSAGPKPKKSVALSGVTAGNTALCTVGRTGNDLHYRGYDILDVADAC
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCEECCCCHHHHHHHC
EFEEIAHLLVHGKLPNRAELAAYKTKLKSLRGLPANVKAALEWVPASAHPMDVMRTGVSV
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHH
LGTVLPEKDDHNAPGARDIADRLMASLGSMLLYWYHYSHNGRRIEVETDDDSIGAHFLHL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHH
LHGEKPSKLWERAMHTSMILYAEHEFNASTFTGRVIAGTGSDMYSAICGAIGALRGPKHG
HCCCCHHHHHHHHHHHEEEEEEECCCCCCEECCEEEECCCCHHHHHHHHHHHHCCCCCCC
GANEVAFEIQKRYDSPDEAQADIARRVENKEVVIGFGHPVYTIADPRNQVIKETARNLSK
CCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHH
DAGSMKMFDIAERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHEEEEEECCCHHH
HIIEQRIDNKIIRPSANYTGPEDLKFVPIGKRK
HHHHHHHCCCEECCCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure 
SAANHTTPADAPQSAGPKPKKSVALSGVTAGNTALCTVGRTGNDLHYRGYDILDVADAC
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCEECCCCHHHHHHHC
EFEEIAHLLVHGKLPNRAELAAYKTKLKSLRGLPANVKAALEWVPASAHPMDVMRTGVSV
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHH
LGTVLPEKDDHNAPGARDIADRLMASLGSMLLYWYHYSHNGRRIEVETDDDSIGAHFLHL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHH
LHGEKPSKLWERAMHTSMILYAEHEFNASTFTGRVIAGTGSDMYSAICGAIGALRGPKHG
HCCCCHHHHHHHHHHHEEEEEEECCCCCCEECCEEEECCCCHHHHHHHHHHHHCCCCCCC
GANEVAFEIQKRYDSPDEAQADIARRVENKEVVIGFGHPVYTIADPRNQVIKETARNLSK
CCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHH
DAGSMKMFDIAERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIARTSGWAA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHEEEEEECCCHHH
HIIEQRIDNKIIRPSANYTGPEDLKFVPIGKRK
HHHHHHHCCCEECCCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]