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The map label for this gene is gltA [H]

Identifier: 73542006

GI number: 73542006

Start: 2559414

End: 2560715

Strand: Reverse

Name: gltA [H]

Synonym: Reut_A2320

Alternate gene names: 73542006

Gene position: 2560715-2559414 (Counterclockwise)

Preceding gene: 73542007

Following gene: 73542005

Centisome position: 67.27

GC content: 61.98

Gene sequence:

>1302_bases
ATGACGCCGTCCGATGTGAAAGCCACGCTATCGTTCTCCGATGGTTCCCCCAGCGTTGAGCTGCCGATCTACAAGGGCAC
CGTAGGCCCGGACGTGATCGACATTCGCAAGCTGTACGGCCAGACCGGCAAGTTCACCTACGACCCCGGTTTCATGTCGA
CGGCTTCGTGCAATTCGAAGATCACCTACATCGACGGCGACAAGGGCGAGCTGCTGTACCGCGGCTACCCGATCGAGCAA
CTGGCGCAGAAGTGCGACCACCTCGAAACCTGCTACCTGTTGCTGAAGGGCGAACTGCCCAACGCCAAGCAGAAGGAAGA
ATTCACCGGCCACGTCATGAACCACACCATGGTTCACGAGCAGCTGCAGTTCTTCCTGCGCGGTTTCCGCCGCGATGCGC
ACCCGATGGCCGTGCTGACCGGCCTGGTCGGTGCCATGAGCGCGTTCTACCACGACGCGATGGACATCGATGATCCGCAC
CAGCGCGAGATCTCGGCCATCCGCCTGATCGCCAAGATGCCGACCATGGTCGCCATGGCGTACAAGTACAACATCGGCCA
GCCGTACATCTACCCGCAGAACGACCTGTCCTACTCGGGCAACTTCCTGCGCATGCTGTTCGGCACGCCGTGCGCCCCGT
ACACCGTGAACCCGGTGCTGGAGCGTGCGCTGGACCGAATCTTCATCCTGCACGCCGACCACGAGCAGAACGCTTCGACC
TCGACCGTGCGTCTGGCCGGTTCGTCGGGTACGAACCCCTTCGCAGCGATCGCCGCTGGCGTGGCCTGCCTGTGGGGTCC
GGCTCACGGCGGCGCCAACGAAGCCGCGCTGAAGATGCTGGAAGAGATCGGCAGCGTCGACAACATCGGCGAGTTCATCA
AGCAGGTCAAGGACAAGAACTCGGGCGTGCGCCTGATGGGCTTTGGCCACCGCGTGTACAAGAACTACGATCCGCGCGCC
AAGCTGATGCGCGAAACCTGCCATGAAGTGCTGAACGAACTGGGCCTGCACAACGACCCGCTGTTCAAGCTCGCCATGGA
GCTGGAAAAGATCGCGCTGGAAGACGAATACTTCGTCAGCCGCAAGCTGTACCCGAACGTCGACTTCTACTCGGGCATCG
TCCAGCGCGCGCTGGGTATCCCGACGTCGCTGTTCACCTGCATCTTCGCGCTGGCTCGTACCCCGGGCTGGATCTCGCAG
TGGGAAGAGATGATCACCGATCCGGAATACAAGATCGGCCGTCCGCGCCAGCTGTTCAACGGCGCCGCTTCGCGCGACGT
GCCGGACATTTCCAAGCGCTAA

Upstream 100 bases:

>100_bases
GTGAGCTCGACACGCCCCCGATGCAGCGCATCATTGGCCTGCTGCGTTCGGTCTGAGTATTTGGTCAATATTATTATTCA
CAGCATTTGAAGGATCCGAC

Downstream 100 bases:

>100_bases
GCGCGGGAATCGCTCTGGCTGCGCACCGCAGCAATCCCAAACGCCCCGGCTCGCCGGGGCGTTTGTCTTTCCGGCGTCAC
GCGTCTTGTGCGATGCAGCG

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTPSDVKATLSFSDGSPSVELPIYKGTVGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSKITYIDGDKGELLYRGYPIEQ
LAQKCDHLETCYLLLKGELPNAKQKEEFTGHVMNHTMVHEQLQFFLRGFRRDAHPMAVLTGLVGAMSAFYHDAMDIDDPH
QREISAIRLIAKMPTMVAMAYKYNIGQPYIYPQNDLSYSGNFLRMLFGTPCAPYTVNPVLERALDRIFILHADHEQNAST
STVRLAGSSGTNPFAAIAAGVACLWGPAHGGANEAALKMLEEIGSVDNIGEFIKQVKDKNSGVRLMGFGHRVYKNYDPRA
KLMRETCHEVLNELGLHNDPLFKLAMELEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPTSLFTCIFALARTPGWISQ
WEEMITDPEYKIGRPRQLFNGAASRDVPDISKR

Sequences:

>Translated_433_residues
MTPSDVKATLSFSDGSPSVELPIYKGTVGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSKITYIDGDKGELLYRGYPIEQ
LAQKCDHLETCYLLLKGELPNAKQKEEFTGHVMNHTMVHEQLQFFLRGFRRDAHPMAVLTGLVGAMSAFYHDAMDIDDPH
QREISAIRLIAKMPTMVAMAYKYNIGQPYIYPQNDLSYSGNFLRMLFGTPCAPYTVNPVLERALDRIFILHADHEQNAST
STVRLAGSSGTNPFAAIAAGVACLWGPAHGGANEAALKMLEEIGSVDNIGEFIKQVKDKNSGVRLMGFGHRVYKNYDPRA
KLMRETCHEVLNELGLHNDPLFKLAMELEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPTSLFTCIFALARTPGWISQ
WEEMITDPEYKIGRPRQLFNGAASRDVPDISKR
>Mature_432_residues
TPSDVKATLSFSDGSPSVELPIYKGTVGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSKITYIDGDKGELLYRGYPIEQL
AQKCDHLETCYLLLKGELPNAKQKEEFTGHVMNHTMVHEQLQFFLRGFRRDAHPMAVLTGLVGAMSAFYHDAMDIDDPHQ
REISAIRLIAKMPTMVAMAYKYNIGQPYIYPQNDLSYSGNFLRMLFGTPCAPYTVNPVLERALDRIFILHADHEQNASTS
TVRLAGSSGTNPFAAIAAGVACLWGPAHGGANEAALKMLEEIGSVDNIGEFIKQVKDKNSGVRLMGFGHRVYKNYDPRAK
LMRETCHEVLNELGLHNDPLFKLAMELEKIALEDEYFVSRKLYPNVDFYSGIVQRALGIPTSLFTCIFALARTPGWISQW
EEMITDPEYKIGRPRQLFNGAASRDVPDISKR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=423, Percent_Identity=28.1323877068558, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786939, Length=430, Percent_Identity=65.3488372093023, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1786527, Length=384, Percent_Identity=28.3854166666667, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17555174, Length=412, Percent_Identity=27.6699029126214, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319850, Length=340, Percent_Identity=27.9411764705882, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=373, Percent_Identity=25.7372654155496, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6325257, Length=369, Percent_Identity=27.3712737127371, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640124, Length=427, Percent_Identity=27.4004683840749, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24640126, Length=425, Percent_Identity=27.5294117647059, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356863, Length=426, Percent_Identity=27.4647887323944, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48430; Mature: 48299

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSDVKATLSFSDGSPSVELPIYKGTVGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSK
CCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCEECCCCCE
ITYIDGDKGELLYRGYPIEQLAQKCDHLETCYLLLKGELPNAKQKEEFTGHVMNHTMVHE
EEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHH
QLQFFLRGFRRDAHPMAVLTGLVGAMSAFYHDAMDIDDPHQREISAIRLIAKMPTMVAMA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YKYNIGQPYIYPQNDLSYSGNFLRMLFGTPCAPYTVNPVLERALDRIFILHADHEQNAST
HHCCCCCCEECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCC
STVRLAGSSGTNPFAAIAAGVACLWGPAHGGANEAALKMLEEIGSVDNIGEFIKQVKDKN
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCC
SGVRLMGFGHRVYKNYDPRAKLMRETCHEVLNELGLHNDPLFKLAMELEKIALEDEYFVS
CCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
RKLYPNVDFYSGIVQRALGIPTSLFTCIFALARTPGWISQWEEMITDPEYKIGRPRQLFN
HCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHC
GAASRDVPDISKR
CCCCCCCCCCCCC
>Mature Secondary Structure 
TPSDVKATLSFSDGSPSVELPIYKGTVGPDVIDIRKLYGQTGKFTYDPGFMSTASCNSK
CCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCEECCCCCE
ITYIDGDKGELLYRGYPIEQLAQKCDHLETCYLLLKGELPNAKQKEEFTGHVMNHTMVHE
EEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHH
QLQFFLRGFRRDAHPMAVLTGLVGAMSAFYHDAMDIDDPHQREISAIRLIAKMPTMVAMA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YKYNIGQPYIYPQNDLSYSGNFLRMLFGTPCAPYTVNPVLERALDRIFILHADHEQNAST
HHCCCCCCEECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCC
STVRLAGSSGTNPFAAIAAGVACLWGPAHGGANEAALKMLEEIGSVDNIGEFIKQVKDKN
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCC
SGVRLMGFGHRVYKNYDPRAKLMRETCHEVLNELGLHNDPLFKLAMELEKIALEDEYFVS
CCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
RKLYPNVDFYSGIVQRALGIPTSLFTCIFALARTPGWISQWEEMITDPEYKIGRPRQLFN
HCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHC
GAASRDVPDISKR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]