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The map label for this gene is braC [H]

Identifier: 73542005

GI number: 73542005

Start: 2557771

End: 2558886

Strand: Reverse

Name: braC [H]

Synonym: Reut_A2319

Alternate gene names: 73542005

Gene position: 2558886-2557771 (Counterclockwise)

Preceding gene: 73542006

Following gene: 73542004

Centisome position: 67.22

GC content: 63.44

Gene sequence:

>1116_bases
ATGACGCTTTCCCGCCTTACGTCCATTTCGTTGGCCGCCGTGCTGGCCACCCTTGGCGCTGCCGCCAACGCCGAAACCGT
GAAGATCGCTATTGCCGGCCCGATGAGCGGCGCCGTGGCGCAGTATGGCGATATGGTCAAGGCCGGTGCGCTGACCGCCA
TTGAACAGATCAACGCAGCCGGTGGTGCCGGCGGCAACAAGTTCGAGGCGGTGATGATGGATGATGCCTGCGAGCCGAAG
CAGGCCGTGGCCGTGGCCAACAAGATCGTCAGCCAGGGCATTAAGTACGTCATTGGCCATGTGTGCTCGGGCTCGACCAT
CCCGGCTTCGGATATCTACGAGAACGAAGGCATTGTGATGGTCACGCCGTCGGCCACCGCACCGCAACTGACCGAAGCGA
AAAAGCGCAAGTTCATCTTCCGCACCATCGGCCGCGATGACCAGCAGGGCCCGGCTGCCGCCCAGTACATCATCAGCAAG
GTCAAGCCGAAGAAGGTCGCCGTGCTGCACGACAAGCAGTCCTACGGCCAGGGCATCGCTACCTCGGTGAAGAGGGACCT
GGAAGCCGCCAAGATCCCGGTGGCGCTGTTTGAAGGCATCAACGCCGGCGATTCGGATTACTCCGCCGTGATCACGAAGC
TGAAGTCGCAGGGCGTGGATTTCGTCTACTTCGGCGGTTACCACCCGGAAATGGGCCTGCTGATGCGCCAGGCGCGCGAG
CAGGGCGTGAAGGCCACGTTCATGGGCCCCGAGGGCGTGGGCAACAAGGACGTCACCGCGATTGCGGGCCCGGCTTCCGA
AGGCATGCTGGTGACGCTGCCGGCCGACTTCTCGGCCGACCCTGCCAACGCAGGCCTGGTGAAGGCGTTCGCCGACAAGA
AGCGTGATGCCAACGGCCCGTTCCAGATGCCGTCCTACGCCGCCGTCAAGATCCTCGGCGACGCCATCGCCGGCGCCAAG
AGCACCGATCCGACCAAGGTCGCGGCGTACATGCACAAGAACGCCTTCCAGACCCCGATCGGCAAGGTCGAGTACGACGA
CAAGGGCGACCTGAAGTCCTTCAAGTTCGTGGTCTACACCTGGCACAAGGACGCCAGCAAGACCGCCGCCAACTGA

Upstream 100 bases:

>100_bases
CTCGGACCTGGTTTCTCTGTCGTCCGCGGGGTGTAGTTCGATTGCGCAGGTCATGCCGTCTCTTGCCCGTATGACCACAA
TACCAATCGAGGAGCTCCTG

Downstream 100 bases:

>100_bases
TCCCGGCCTTTCCTTCGTAGCCATCCCGCGCCTCCACAAGGGGCCGCGGGATGGTCCGCTATCGGGCTTCGGCGCGGGCT
TTCAACCGGGCTTCGACTTC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTLSRLTSISLAAVLATLGAAANAETVKIAIAGPMSGAVAQYGDMVKAGALTAIEQINAAGGAGGNKFEAVMMDDACEPK
QAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGIVMVTPSATAPQLTEAKKRKFIFRTIGRDDQQGPAAAQYIISK
VKPKKVAVLHDKQSYGQGIATSVKRDLEAAKIPVALFEGINAGDSDYSAVITKLKSQGVDFVYFGGYHPEMGLLMRQARE
QGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAGLVKAFADKKRDANGPFQMPSYAAVKILGDAIAGAK
STDPTKVAAYMHKNAFQTPIGKVEYDDKGDLKSFKFVVYTWHKDASKTAAN

Sequences:

>Translated_371_residues
MTLSRLTSISLAAVLATLGAAANAETVKIAIAGPMSGAVAQYGDMVKAGALTAIEQINAAGGAGGNKFEAVMMDDACEPK
QAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGIVMVTPSATAPQLTEAKKRKFIFRTIGRDDQQGPAAAQYIISK
VKPKKVAVLHDKQSYGQGIATSVKRDLEAAKIPVALFEGINAGDSDYSAVITKLKSQGVDFVYFGGYHPEMGLLMRQARE
QGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAGLVKAFADKKRDANGPFQMPSYAAVKILGDAIAGAK
STDPTKVAAYMHKNAFQTPIGKVEYDDKGDLKSFKFVVYTWHKDASKTAAN
>Mature_370_residues
TLSRLTSISLAAVLATLGAAANAETVKIAIAGPMSGAVAQYGDMVKAGALTAIEQINAAGGAGGNKFEAVMMDDACEPKQ
AVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGIVMVTPSATAPQLTEAKKRKFIFRTIGRDDQQGPAAAQYIISKV
KPKKVAVLHDKQSYGQGIATSVKRDLEAAKIPVALFEGINAGDSDYSAVITKLKSQGVDFVYFGGYHPEMGLLMRQAREQ
GVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAGLVKAFADKKRDANGPFQMPSYAAVKILGDAIAGAKS
TDPTKVAAYMHKNAFQTPIGKVEYDDKGDLKSFKFVVYTWHKDASKTAAN

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=349, Percent_Identity=53.5816618911175, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI48994941, Length=347, Percent_Identity=51.8731988472622, Blast_Score=376, Evalue=1e-105,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 38844; Mature: 38713

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSRLTSISLAAVLATLGAAANAETVKIAIAGPMSGAVAQYGDMVKAGALTAIEQINAA
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
GGAGGNKFEAVMMDDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGIVM
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEE
VTPSATAPQLTEAKKRKFIFRTIGRDDQQGPAAAQYIISKVKPKKVAVLHDKQSYGQGIA
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHCCHHH
TSVKRDLEAAKIPVALFEGINAGDSDYSAVITKLKSQGVDFVYFGGYHPEMGLLMRQARE
HHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHH
QGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAGLVKAFADKKRDANGP
CCCEEEEECCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCC
FQMPSYAAVKILGDAIAGAKSTDPTKVAAYMHKNAFQTPIGKVEYDDKGDLKSFKFVVYT
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEE
WHKDASKTAAN
EECCCCCCCCC
>Mature Secondary Structure 
TLSRLTSISLAAVLATLGAAANAETVKIAIAGPMSGAVAQYGDMVKAGALTAIEQINAA
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
GGAGGNKFEAVMMDDACEPKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGIVM
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEE
VTPSATAPQLTEAKKRKFIFRTIGRDDQQGPAAAQYIISKVKPKKVAVLHDKQSYGQGIA
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHCCHHH
TSVKRDLEAAKIPVALFEGINAGDSDYSAVITKLKSQGVDFVYFGGYHPEMGLLMRQARE
HHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHH
QGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAGLVKAFADKKRDANGP
CCCEEEEECCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCC
FQMPSYAAVKILGDAIAGAKSTDPTKVAAYMHKNAFQTPIGKVEYDDKGDLKSFKFVVYT
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEE
WHKDASKTAAN
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]