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The map label for this gene is leuD

Identifier: 73541998

GI number: 73541998

Start: 2551423

End: 2552073

Strand: Reverse

Name: leuD

Synonym: Reut_A2312

Alternate gene names: 73541998

Gene position: 2552073-2551423 (Counterclockwise)

Preceding gene: 73541999

Following gene: 73541997

Centisome position: 67.04

GC content: 62.37

Gene sequence:

>651_bases
ATGGACAAGTTCACTGTACACAGCGGCCTCGTGGCGCCGCTGGACCGCGAGAACGTCGATACCGACGCCATCATCCCGAA
GCAGTTCCTGAAGTCGATCAAGCGCACCGGCTTCGGCCCGAACCTGTTCGACGAATGGCGCTACAAGGACGTCGGGGAGC
CCGGCATGGACAACAGCAAGCGTCCGCTGAACCCGGACTTCGTGCTGAACCAGCCGCGCTACCAGGGTGCGTCGATCCTG
CTCGCGCGCCGCAACTTCGGCTGCGGCAGCTCGCGCGAACATGCGCCGTGGGCGCTCTCGCAATACGGCTTCCGCGCCGT
GATCGCGCCGAGCTTCGCCGACATCTTCTTCAACAACTGCTACAAGAACGGCCTGCTGCCGGTGGTGCTGAGCGAGCAGC
AAATCGACCACCTGTTCAACGAGACCAACGCGTTCAACGGCTACAAGCTGACCATCGACCTGGACAAGCAGGTCGTGCTC
ACGCCGTCGGGCCAGGGCTATGAGTTCGACATCGCCCCGTTCCGCAAGTACTGCATGCTGAATGGCTTTGACGATATCGG
CCTGACCCTGCGCCATGCCGACAAGATCAAGAGCTATGAAGCCGAGCGCGTGGCGAAGATGCCGTGGCTGAACAACCGCG
TGGTCGGCTGA

Upstream 100 bases:

>100_bases
CTGCAACTGGCCGGCTGCAACACCATGGCCGGTCTGGGCAAGGACACGCAGGCCGCCGGCAGCTCGCTCGAGCGCGCGGC
CAAGAAGTAAGGAAAGAGAC

Downstream 100 bases:

>100_bases
ACGCCACAGCGGAAATCAAACAAGGAAAACACAGCATGAAGATCGCAGTCCTGCCAGGTGACGGCATCGGTCCGGAAATC
GTTGCCGAGGCCGTCAAGGT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MDKFTVHSGLVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNLFDEWRYKDVGEPGMDNSKRPLNPDFVLNQPRYQGASIL
LARRNFGCGSSREHAPWALSQYGFRAVIAPSFADIFFNNCYKNGLLPVVLSEQQIDHLFNETNAFNGYKLTIDLDKQVVL
TPSGQGYEFDIAPFRKYCMLNGFDDIGLTLRHADKIKSYEAERVAKMPWLNNRVVG

Sequences:

>Translated_216_residues
MDKFTVHSGLVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNLFDEWRYKDVGEPGMDNSKRPLNPDFVLNQPRYQGASIL
LARRNFGCGSSREHAPWALSQYGFRAVIAPSFADIFFNNCYKNGLLPVVLSEQQIDHLFNETNAFNGYKLTIDLDKQVVL
TPSGQGYEFDIAPFRKYCMLNGFDDIGLTLRHADKIKSYEAERVAKMPWLNNRVVG
>Mature_216_residues
MDKFTVHSGLVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNLFDEWRYKDVGEPGMDNSKRPLNPDFVLNQPRYQGASIL
LARRNFGCGSSREHAPWALSQYGFRAVIAPSFADIFFNNCYKNGLLPVVLSEQQIDHLFNETNAFNGYKLTIDLDKQVVL
TPSGQGYEFDIAPFRKYCMLNGFDDIGLTLRHADKIKSYEAERVAKMPWLNNRVVG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=206, Percent_Identity=57.7669902912621, Blast_Score=239, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6321429, Length=215, Percent_Identity=49.7674418604651, Blast_Score=198, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_CUPPJ (Q46YV9)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296518.1
- ProteinModelPortal:   Q46YV9
- SMR:   Q46YV9
- GeneID:   3609018
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2312
- NMPDR:   fig|264198.3.peg.3042
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   REUT264198:REUT_A2312-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 24542; Mature: 24542

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFTVHSGLVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNLFDEWRYKDVGEPGMDNSK
CCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCC
RPLNPDFVLNQPRYQGASILLARRNFGCGSSREHAPWALSQYGFRAVIAPSFADIFFNNC
CCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCEEHHHCCCEEEECCHHHHHHHHHH
YKNGLLPVVLSEQQIDHLFNETNAFNGYKLTIDLDKQVVLTPSGQGYEFDIAPFRKYCML
HCCCCEEEEECHHHHHHHHHHCCCCCCEEEEEEECCEEEECCCCCCEEEECHHHHHHHHH
NGFDDIGLTLRHADKIKSYEAERVAKMPWLNNRVVG
CCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDKFTVHSGLVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNLFDEWRYKDVGEPGMDNSK
CCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCC
RPLNPDFVLNQPRYQGASILLARRNFGCGSSREHAPWALSQYGFRAVIAPSFADIFFNNC
CCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCEEHHHCCCEEEECCHHHHHHHHHH
YKNGLLPVVLSEQQIDHLFNETNAFNGYKLTIDLDKQVVLTPSGQGYEFDIAPFRKYCML
HCCCCEEEEECHHHHHHHHHHCCCCCCEEEEEEECCEEEECCCCCCEEEECHHHHHHHHH
NGFDDIGLTLRHADKIKSYEAERVAKMPWLNNRVVG
CCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA